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1. 发明授权

US08710207B2 Compositions, organisms, systems, and methods for expressing a gene product in plants using SCBV expression control sequences operable in monocots and dicots 有权
标题翻译：使用在单子叶植物和双子叶植物中可操作的SCBV表达调控序列在植物中表达基因产物的组合物，生物体系，系统和方法
公开(公告)号：US08710207B2
公开(公告)日：2014-04-29
申请号：US13104158
申请日：2011-05-10
申请人： T. Erik Mirkov , Jong Won Park , San-Ji Gao
发明人： T. Erik Mirkov , Jong Won Park , San-Ji Gao
IPC分类号： C07H21/04 , C12N1/20 , C12N15/11 , C12N15/63 , C12N15/79 , C12N15/82 , A01H4/00 , A01H5/00
CPC分类号： C12N15/8216 , C12N9/2462 , C12N15/52 , C12N15/8222 , C12N15/8237 , C12N15/8241
摘要： The present disclosure relates, in some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant using a expression control sequence (ECS) operable in monocots and/or dicots. For example, (i) an isolated nucleic acid may comprise an ECS (e.g., a sugarcane bacilliform virus promoter) and, optionally, an exogenous nucleic acid (ExNA) operably linked to the ECS; (ii) an expression vector may comprise an ECS; an ExNA; and, optionally, a 3′ termination sequence, wherein the ECS has promoter activity sufficient to express the ExNA in at least one monocot and at least one dicot; (iii) a microorganism, plant cell, or plant may comprise an isolated nucleic acid; (iv) a method for constitutively expressing an ExNA in a plant (e.g., a monocot and/or a dicot) may comprise, contacting an expression vector with the cytosol of a cell of the plant, wherein the expression vector comprises the ExNA and an ECS operable to drive expression of the ExNA; and/or (v) a method of directing constitutive expression of a nucleic acid in a plant (e.g., a monocot and/or a dicot) may comprise transforming the plant with an expression nucleic acid comprising an ECS, an ExNA, and a 3′ termination sequence.
摘要翻译：在一些实施方案中，本公开涉及使用在单子叶植物和/或双子叶植物中可操作的表达调控序列（ECS）在植物中表达基因产物的组合物，生物，系统和方法。例如，（i）分离的核酸可以包含可操作地连接到ECS的ECS（例如，甘蔗杆状病毒启动子）和任选的外源核酸（ExNA）; （ii）表达载体可以包含ECS; 一个ExNA; 和任选的3'终止序列，其中ECS具有足以在至少一个单子叶植物和至少一个双子叶植物中表达ExNA的启动子活性; （iii）微生物，植物细胞或植物可以包含分离的核酸; （iv）在植物（例如单子叶植入物和/或双子叶植物）中组成型表达ExNA的方法可以包括使表达载体与植物细胞的细胞溶质接触，其中表达载体包含ExNA和 ECS可以推动ExNA的表达; 和/或（v）指导核酸在植物（例如单子叶植物和/或双子叶植物）中的组成型表达的方法可包括用包含ECS，ExNA和3的表达核酸转化植物 '终止序列。

2. 发明申请

US20110283377A1 COMPOSITIONS, ORGANISMS, SYSTEMS, AND METHODS FOR EXPRESSING A GENE PRODUCT IN PLANTS 有权
标题翻译：用于在植物中表达基因产物的组合物，有机体，系统和方法
公开(公告)号：US20110283377A1
公开(公告)日：2011-11-17
申请号：US13104158
申请日：2011-05-10
申请人： T. Erik Mirkov , Jong Won Park , San-Ji Gao
发明人： T. Erik Mirkov , Jong Won Park , San-Ji Gao
IPC分类号： A01H5/00 , C12N15/29 , A01H1/02 , C12N1/21 , C12N5/10 , C12N15/82 , C12N15/113 , C12N15/63
CPC分类号： C12N15/8216 , C12N9/2462 , C12N15/52 , C12N15/8222 , C12N15/8237 , C12N15/8241
摘要： The present disclosure relates, in some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant using a expression control sequence (ECS) operable in monocots and/or dicots. For example, (i) an isolated nucleic acid may comprise an ECS (e.g., a sugarcane bacilliform virus promoter) and, optionally, an exogenous nucleic acid (ExNA) operably linked to the ECS; (ii) an expression vector may comprise an ECS; an ExNA; and, optionally, a 3′ termination sequence, wherein the ECS has promoter activity sufficient to express the ExNA in at least one monocot and at least one dicot; (iii) a microorganism, plant cell, or plant may comprise an isolated nucleic acid; (iv) a method for constitutively expressing an ExNA in a plant (e.g., a monocot and/or a dicot) may comprise, contacting an expression vector with the cytosol of a cell of the plant, wherein the expression vector comprises the ExNA and an ECS operable to drive expression of the ExNA; and/or (v) a method of directing constitutive expression of a nucleic acid in a plant (e.g., a monocot and/or a dicot) may comprise transforming the plant with an expression nucleic acid comprising an ECS, an ExNA, and a 3′ termination sequence.
摘要翻译：在一些实施方案中，本公开涉及使用在单子叶植物和/或双子叶植物中可操作的表达调控序列（ECS）在植物中表达基因产物的组合物，生物，系统和方法。例如，（i）分离的核酸可以包含可操作地连接到ECS的ECS（例如，甘蔗杆状病毒启动子）和任选的外源核酸（ExNA）; （ii）表达载体可以包含ECS; 一个ExNA; 和任选的3'终止序列，其中ECS具有足以在至少一个单子叶植物和至少一个双子叶植物中表达ExNA的启动子活性; （iii）微生物，植物细胞或植物可以包含分离的核酸; （iv）在植物（例如单子叶植入物和/或双子叶植物）中组成型表达ExNA的方法可以包括使表达载体与植物细胞的细胞溶质接触，其中表达载体包含ExNA和 ECS可以推动ExNA的表达; 和/或（v）指导核酸在植物（例如单子叶植物和/或双子叶植物）中的组成型表达的方法可包括用包含ECS，ExNA和3的表达核酸转化植物 '终止序列。

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