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1. 发明授权

US5663049A Detection of campylobacter 失效
标题翻译：检测弯曲杆菌
公开(公告)号：US5663049A
公开(公告)日：1997-09-02
申请号：US453756
申请日：1995-05-30
申请人： Susan M. Barns , Ray A. McMillian , David J. Lane , Mark L. Collins , James E. Awell , Ayoub Rashtchian
发明人： Susan M. Barns , Ray A. McMillian , David J. Lane , Mark L. Collins , James E. Awell , Ayoub Rashtchian
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N15/00 , C12Q1/04 , C12Q1/68 , C12R1/01 , G01N33/50 , C07H21/02
CPC分类号： C12Q1/6813 , C12Q1/689
摘要： The invention relates to methods of using nucleic acid probes capable of specifically hybridizing to rRNA of Campylobacter jejuni, C. coli and C. laridis and not to rRNA or rRNA genes of Pseudomonas aeuroginosa, E. coli or Salmonella typhimunium for the detection of Campylobacter in clinical, food and other samples.
摘要翻译：本发明涉及使用能够与空肠弯曲杆菌，大肠杆菌和C.laridis的rRNA特异性杂交的核酸探针的方法，而不涉及用于检测弯曲杆菌的弯曲杆菌的假单胞菌（Pseudomonas aeuroginosa），大肠杆菌（Salmonella typhimunium）的rRNA或rRNA基因临床，食品等样品。

2. 发明授权

US5447848A Detection of campylobacter with nucleic acid probes 失效
标题翻译：用核酸探针检测弯曲杆菌
公开(公告)号：US5447848A
公开(公告)日：1995-09-05
申请号：US216679
申请日：1988-07-07
申请人： Susan M. Barns , Ray A. McMillian , David J. Lane , Mark L. Collins , James E. Awell , Ayoub Rashtchian
发明人： Susan M. Barns , Ray A. McMillian , David J. Lane , Mark L. Collins , James E. Awell , Ayoub Rashtchian
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N15/00 , C12Q1/04 , C12Q1/68 , C12R1/01 , G01N33/50 , C07H21/00 , C12Q1/02
CPC分类号： C12Q1/6813 , C12Q1/689
摘要： Nucleic acid probes capable of specifically hybridizing to rRNA of Campylobacter jejuni, C. coli and C. laridis and not to rRNA or rRNA genes of Pseudomonas aeuroginosa, E. coli or Salmonella typhimunium are described along with methods utilizing such probes for the detection of Campylobacter in clinical, food and other samples.
摘要翻译：与能够与空肠弯曲杆菌，大肠杆菌和C.laridis的rRNA特异性杂交的核酸探针以及不属于恶臭假单胞菌（Pseudomonas aeuroginosa），大肠杆菌或鼠伤寒沙门氏菌的rRNA或rRNA基因的核酸探针与使用这种探针用于检测弯曲杆菌在临床，食品等样品中。

3. 发明授权

US5495008A Oligonucleotide probes for detection of salmonella 失效
标题翻译：用于检测沙门氏菌的寡核苷酸探针
公开(公告)号：US5495008A
公开(公告)日：1996-02-27
申请号：US870804
申请日：1992-04-17
申请人： David J. Lane , Ayoub Rashtchian , Kyriaki Parodos
发明人： David J. Lane , Ayoub Rashtchian , Kyriaki Parodos
IPC分类号： C12N15/09 , C12Q1/10 , C12Q1/68 , C12R1/42 , C07H21/04
CPC分类号： C12Q1/689 , C12Q1/6813
摘要： Nucleic acid fragments capable of hybridizing to rRNA of a Salmonella species and not capable of hybridizing to rRNA of Escherichia coli.
摘要翻译：能够与沙门氏菌属物种的rRNA杂交并且不能与大肠杆菌的rRNA杂交的核酸片段。

4. 发明授权

US5792854A Detection of salmonella 失效
标题翻译：检测沙门氏菌
公开(公告)号：US5792854A
公开(公告)日：1998-08-11
申请号：US465987
申请日：1995-06-06
申请人： David J. Lane , Ayoub Rashtchian , Kyriaki Parodos
发明人： David J. Lane , Ayoub Rashtchian , Kyriaki Parodos
IPC分类号： C12N15/09 , C12Q1/10 , C12Q1/68 , C12R1/42 , C07H21/04 , C12N15/10 , C12N15/31
CPC分类号： C12Q1/689 , C12Q1/6813
摘要： Nucleic acid fragments capable of hybridizing to rRNA of a Salmonella species and not capable of hybridizing to rRNA of Escherichia coli.
摘要翻译：能够与沙门氏菌属物种的rRNA杂交并且不能与大肠杆菌的rRNA杂交的核酸片段。

5. 发明申请

US20110151520A1 Compositions and methods for cDNA synthesis 有权
标题翻译： cDNA合成的组合物和方法
公开(公告)号：US20110151520A1
公开(公告)日：2011-06-23
申请号：US12632802
申请日：2009-12-08
申请人： Ayoub Rashtchian , David Schuster
发明人： Ayoub Rashtchian , David Schuster
IPC分类号： C12P19/34 , C12N9/12
CPC分类号： C12P19/34 , C12N15/1096 , C12Q1/6846 , C12Q2527/143 , C12Q2525/179 , C12Q2521/107
摘要： Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided.Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.
摘要翻译：提供了制备cDNA分子，通过PCR扩增RNA和制备cDNA文库的方法。还提供了制备cDNA分子的试剂盒。还提供组合物，其包括试剂的混合物，包括逆转录酶，缓冲液，辅因子和其它组分，适于立即用于将RNA转化成cDNA和RT PCR，而不进行稀释或添加其它组分。这些组合物可单独或以试剂盒的形式用于cDNA合成或核酸扩增（例如，通过聚合酶链式反应）或在各种研究，医学，诊断，法医和农业应用中使用逆转录酶的任何方法。

6. 发明申请

US20080131938A1 USE OF PREDETERMINED NUCLEOTIDES HAVING ALTERED BASE PAIRING CHARACTERISTICS IN THE AMPLIFICATION OF NUCLEIC ACID MOLECULES 失效
标题翻译：在核酸分子扩增中使用具有改变的碱基配对特性的预测核苷酸
公开(公告)号：US20080131938A1
公开(公告)日：2008-06-05
申请号：US11768865
申请日：2007-06-26
申请人： David M. Schuster , Ayoub Rashtchian
发明人： David M. Schuster , Ayoub Rashtchian
IPC分类号： C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6832 , C12Q1/6853 , C12Q1/686 , C12Q1/6862 , C12Q2600/156 , C12Q2527/107 , C12Q2525/101 , C12Q2521/107
摘要： The present invention provides improved methods for amplifying a nucleic acid molecule. More specifically, the invention provides methods for nucleic acid amplification which use primers having equivalent priming efficiency.
摘要翻译：本发明提供了用于扩增核酸分子的改进方法。更具体地，本发明提供了使用具有等效启动效率的引物的核酸扩增方法。

7. 发明授权

US06495350B1 Compositions comprising a M-MLV reverse transcriptase and a DNA polymerase and use thereof 有权
标题翻译：包含M-MLV逆转录酶和DNA聚合酶的组合物及其用途
公开(公告)号：US06495350B1
公开(公告)日：2002-12-17
申请号：US09472066
申请日：1999-12-23
申请人： Jun E. Lee , Ayoub Rashtchian
发明人： Jun E. Lee , Ayoub Rashtchian
IPC分类号： C12Q168
CPC分类号： C12P19/34 , C12N9/1276 , C12N15/1096 , C12Q1/6846 , C12Q2527/149 , C12Q2527/125 , C12Q2521/107
摘要： The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.
摘要翻译：本发明涉及通过逆转录酶 - 聚合酶链反应（RT-PCR）扩增核酸分子的组合物和方法。具体地，本发明提供了使用逆转录酶和热稳定DNA聚合酶与含硫分子或含乙酸酯分子结合的简化的一步或两步RT-PCR方法扩增核酸分子的组合物和方法（或这种含硫分子和含乙酸酯的分子的组合）和任选的牛血清白蛋白。因此，本发明促进了核酸分子的快速和有效的扩增以及RNA分子的检测和定量。本发明也可用于快速生产和扩增可用于各种工业，医学和法医目的的cDNA。

8. 发明授权

US5578467A Use of deoxyinosine containing primers to balance primer efficiency in the amplification of nucleic acid molecules 失效
标题翻译：使用含有脱氧蛋白的引物平衡引物在核酸分子扩增中的效率
公开(公告)号：US5578467A
公开(公告)日：1996-11-26
申请号：US246921
申请日：1994-05-20
申请人： David M. Schuster , Ayoub Rashtchian
发明人： David M. Schuster , Ayoub Rashtchian
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6832 , C12Q1/6853 , C12Q1/686 , C12Q1/6862 , C12Q2600/156
摘要： The present invention provides improved methods for amplifying a nucleic acid molecule. Specifically, the invention involves replacing at least one nucleotide of an oligonucleotide with a deoxyinosine residue, so as to more equalize the efficiency with which that oligonucloetide and a second oligonucleotide hybridize to a target molecule, and then amplifying the target molecule using, for example, the polymerase chain reaction. Improved amplification results from the improvement in the relative hybridization efficiencies.
摘要翻译：本发明提供了用于扩增核酸分子的改进方法。具体地说，本发明涉及用脱氧肌苷残基替换寡核苷酸的至少一个核苷酸，从而更均衡该寡核苷酸与第二寡核苷酸与目标分子杂交的效率，然后使用例如，聚合酶链反应。改进的扩增来自相对杂交效率的提高。

9. 发明授权

US08372604B2 Compositions and methods for reverse transcriptase-polymerase chain reaction (RT-PCR) 有权
公开(公告)号：US08372604B2
公开(公告)日：2013-02-12
申请号：US12981240
申请日：2010-12-29
申请人： Jun Lee , Ayoub Rashtchian
发明人： Jun Lee , Ayoub Rashtchian
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12N9/1276 , C12N15/1096 , C12Q1/6846 , C12Q2527/149 , C12Q2527/125 , C12Q2521/107
摘要： The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

10. 发明申请

US20090092990A1 PRIMERS AND METHODS FOR THE DETECTION AND DISCRIMINATION OF NUCLEIC ACIDS 审中-公开
标题翻译：用于检测和鉴别核酸的原理和方法
公开(公告)号：US20090092990A1
公开(公告)日：2009-04-09
申请号：US12251378
申请日：2008-10-14
申请人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard M. Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
发明人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard M. Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12P19/34 , C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6869 , G01N21/6486 , C12Q2525/121 , C12Q2525/161 , C12Q2563/107 , C12Q2565/107
摘要： The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.
摘要翻译：本发明提供用于检测特异性核酸序列的新型引物和方法。本发明的引物和方法可用于多种分子生物学应用，并且特别适用于等位基因特异性PCR。

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