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    • 6. 发明申请
    • SYSTEM FOR BIOMARKER DISCOVERY
    • 生物标记发现系统
    • US20060211009A1
    • 2006-09-21
    • US11277033
    • 2006-03-20
    • Sungwhan AnChiwang YoonTae OhMyungsoon Kim
    • Sungwhan AnChiwang YoonTae OhMyungsoon Kim
    • C12Q1/68C07H21/04
    • C12Q1/6886C12Q2600/112C12Q2600/154
    • The present application discloses a method for discovering a methylation marker gene for the conversion of a cell comprising: (i) comparing converted and unconverted cell gene expression content to identify a gene that is present in greater abundance in the unconverted cell; (ii) treating a converted cell with a demethylating agent and comparing its gene expression content with gene expression content of an untreated converted cell to identify a gene that is present in greater abundance in the cell treated with the demethylating agent; and (iii) identifying a gene that is common to the identified genes in steps (i) and (ii), wherein the common identified gene is the methylation marker gene.
    • 本申请公开了一种用于发现用于转化细胞的甲基化标记基因的方法,包括:(i)比较转化和未转化的细胞基因表达含量,以鉴定未转化细胞中更丰富的基因; (ii)用去甲基化剂处理转化的细胞,并将其基因表达含量与未处理的转化细胞的基因表达含量进行比较,以鉴定在用去甲基化试剂处理的细胞中更丰富的基因; 和(iii)鉴定步骤(i)和(ii)中所鉴定的基因共有的基因,其中常见的鉴定基因是甲基化标记基因。