专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20070003944A1 Inference of human geographic origins using Alu insertion polymorphisms 审中-公开
标题翻译：使用Alu插入多态性推测人类地理起源
公开(公告)号：US20070003944A1
公开(公告)日：2007-01-04
申请号：US11202247
申请日：2005-08-12
申请人： Sudhir Sinha , Jaiprakash Shewale , Mark Batzer , Jerilyn Walker , David Ray
发明人： Sudhir Sinha , Jaiprakash Shewale , Mark Batzer , Jerilyn Walker , David Ray
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6888 , C12Q2600/156
摘要： The insertion polymorphisms based on interspersed elements including LINEs and SINEs is used for the inference of an individual's geographic origin. SINE polymorphisms are identical-by-descent, essentially homoplasy-free, and inexpensive to genotype using a variety of approaches. Using a Structure analysis of the Alu insertion polymorphism based genotypes, the geographic affiliation of unknown human individuals can be inferred with high levels of confidence. This technique to infer the geographic affiliation of unknown human DNA samples can be a useful tool in forensic genomics.
摘要翻译：基于散点元素（包括LINE和SINE）的插入多态性被用于个人地理来源的推论。 SINE多态性是同质的，基本上同质的，并且使用各种方法基因型便宜。使用基于Alu插入多态性的基因型的结构分析，可以高度信任地推断未知人类的地理隶属关系。这种用于推断未知人类DNA样本的地理属性的技术可能是法医基因组学中有用的工具。

2. 发明申请

US20060099620A1 Multiplex PCR for simultaneous quantitation of human nuclear, mitochondrial, and male Y-chromosome DNA 有权
标题翻译：用于同时定量人类核，线粒体和雄性Y染色体DNA的多重PCR
公开(公告)号：US20060099620A1
公开(公告)日：2006-05-11
申请号：US11245444
申请日：2005-10-07
申请人： Jerilyn Walker , Dale Hedges , Jaiprakash Shewale , Sudhir Sinha , Mark Batzer
发明人： Jerilyn Walker , Dale Hedges , Jaiprakash Shewale , Sudhir Sinha , Mark Batzer
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6879 , C12Q1/6851 , C12Q1/6888 , C12Q2600/16 , C12Q2537/143
摘要： A comprehensive set of human specific, target specific, multiplex PCR assays for DNA quantitation is provided. Our duplex qPCR for nDNA/mtDNA had a linear quantitation range of 100 ng to 1 pg, and our triplex qPCR assay for nDNA/mtDNA/male Y DNA had a linear range of 100 ng to 0.1 ng. Human-specificity was demonstrated by the accurate detection of 0.05% and 5% human DNA, respectively, from a complex source of starting templates. Target-specificity was confirmed by the lack of cross-amplification among targets. A high throughput alternative for human gender determination was also developed by multiplexing the male Y primer/probe set with an X chromosome based system. Background cross-amplification with DNA templates derived from fourteen other species was negligible aside from the male Y assay which produced spurious amplifications from other non-human primate templates. Mainstream application of these assays will undoubtedly benefit forensic genomics.
摘要翻译：提供了一套全面的人类特异性，靶特异性，多重PCR测定用于DNA定量。我们用于nDNA / mtDNA的双重qPCR线性定量范围为100 ng至1 pg，我们对nDNA / mtDNA /雄性Y DNA的三重qPCR检测线性范围为100 ng至0.1 ng。通过分别从起始模板的复杂来源准确检测0.05％和5％的人类DNA，证明了人的特异性。目标特异性由目标缺乏交叉扩增证实。通过将Y型引物/探针组与基于X染色体的系统进行复用也开发了用于人类性别测定的高通量替代物。来自十四种其他物种的DNA模板的背景交叉扩增除了从其他非人灵长类动物模板产生杂种扩增的雄性Y测定之外，可忽略不计。这些测定的主流应用无疑将有益于法医基因组学。

3. 发明申请

US20050112592A1 Assay for species sources 有权
标题翻译：测定物种来源
公开(公告)号：US20050112592A1
公开(公告)日：2005-05-26
申请号：US10736912
申请日：2003-12-17
申请人： Sudhir Sinha , Jaiprakash Shewale , Jerilyn Walker , Mark Batzer
发明人： Sudhir Sinha , Jaiprakash Shewale , Jerilyn Walker , Mark Batzer
IPC分类号： C07H21/04 , C12Q1/68 , C12P19/34
CPC分类号： C07H21/04 , C12Q1/6888
摘要： A family of PCR assays is disclosed for determining, both qualitatively and quantitatively, presence of material from a predetermined species source and for quantifying the amount of such material. The assays are based respectively on SINEs uniquely characteristic of pig species, cow species, chicken species, and ruminant sub-order, and having a high copy number. The assays disclosed permit rapid, inexpensive evaluation of meat samples to facilitate elimination from their diet of pork or beef by persons desiring to avoid such food sources; as well as the assay of cattle feed to determine presence therein of ruminant-source proteins, which are a potential source of bovine spongiform encephalopathy (BSE), commonly referred to as “mad cow disease.” The assays amplify the predetermined unique SINEs and the resulting amplified mixture is then evaluated qualitatively by electrophoresis on gel containing ethidium bromide or quantitatively by SYBR Green-based detection or TaqMan chemistry. The invention also extends to kits, primers, and other products used in connection with the assays. The amplicons are selected to be from about 100 to 170 bp long.
摘要翻译：公开了一系列PCR测定法，用于确定来自预定物种来源的材料的定性和定量存在以及用于量化此类材料的量。测定分别基于猪种，牛种，鸡种和反刍动物次级的独特特征的SINE，并具有高拷贝数。所公开的测定允许对肉类样品进行快速，便宜的评估，以便于希望避免这种食物来源的人排除猪肉或牛肉的饮食; 以及牛饲料的测定以确定其中的反刍动物源蛋白质，其是牛海绵状脑病（BSE）的潜在来源，通常被称为“疯牛病”。测定法扩增预定的独特SINE，然后通过在含有溴化乙锭的凝胶上电泳定性地定量得到所得扩增的混合物，或定量地通过SYBR Green检测或TaqMan化学。本发明还扩展到试剂盒，引物和与测定结合使用的其它产品。扩增子选择为约100至170bp长。

4. 发明申请

US20050069902A1 Assay for quantitation of human DNA using Alu elements 有权
标题翻译：使用Alu元素测定人类DNA的测定
公开(公告)号：US20050069902A1
公开(公告)日：2005-03-31
申请号：US10673575
申请日：2003-09-30
申请人： Sudhir Sinha , Jerilyn Walker , Mark Batzer
发明人： Sudhir Sinha , Jerilyn Walker , Mark Batzer
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q1/6858 , C12Q2531/113 , C12Q2525/151
摘要： An assay for determining presence of human DNA in a sample in which non-human DNA may also be present and for quantitating such human DNA. One embodiment uses intra-Alu based PCR and another uses inter-Alu based PCR. The assays are performed without unique, expensive equipment. The assays are based on detection of multiple-copy Alu elements recently integrated into the human genome that are largely absent from non-human primates and other mammals.
摘要翻译：用于确定样品中人类DNA存在的测定法，其中也可以存在非人类DNA，并用于定量这样的人类DNA。一个实施方案使用基于Alu的PCR，另一个实施方案使用基于Alu的PCR。无需独特且昂贵的设备进行测定。该测定基于最近整合到人类基因组中的多拷贝Alu元件的检测，其大部分不存在于非人灵长类动物和其他哺乳动物中。

5. 发明申请

US20060141512A1 Novel method for separation of human sperm from biological samples for application in human identification 审中-公开
标题翻译：用于将人类精子与生物样品分离以用于人类鉴定的新方法
公开(公告)号：US20060141512A1
公开(公告)日：2006-06-29
申请号：US11292286
申请日：2005-12-02
申请人： Sudhir Sinha , Jaiprakash Shewale
发明人： Sudhir Sinha , Jaiprakash Shewale
IPC分类号： C12Q1/68
CPC分类号： G01N33/689 , C12Q1/6806 , G01N33/56966 , G01N2400/00 , G01N2800/367
摘要： The development of an isolation methodology for separation of human sperm cells from biological samples containing human epithelial cells is provided. Using sperm binding proteins, glycopeptides, lectins, derivatives of N-acetylglucosamine, triazine dyes or inhibitors of glycosyltransferase linked to an insoluble support, our invention enables binding of human sperm cells from biological samples. Bound sperms can be dissociated and used for in vitro analyses or subsequently lysed on the insoluble support for isolation of male specific DNA. Other cell types such as epithelial cells, white blood cells and cell debris present in the biological samples are not bound to the derivatized insoluble support. The sperm cells, thus isolated, can be processed for isolation of nuclear DNA for human identification and forensic DNA analysis.
摘要翻译：提供了将人精子细胞与含有人上皮细胞的生物样品分离的分离方法的开发。使用精子结合蛋白，糖肽，凝集素，N-乙酰葡糖胺的衍生物，三嗪染料或与不溶性支持物连接的糖基转移酶抑制剂，本发明使得人生精细胞与生物样品结合。结合的精子可以解离并用于体外分析，或随后在不溶性支持物上裂解以分离雄性特异性DNA。存在于生物样品中的其它细胞类型如上皮细胞，白细胞和细胞碎片不与衍生的不溶性支持物结合。这样分离的精子细胞可以被加工用于分离核DNA用于人类鉴定和法医DNA分析。

6. 发明申请

US20060199217A1 Assay for human DNA for gender determination 有权
标题翻译：测定人类DNA进行性别测定
公开(公告)号：US20060199217A1
公开(公告)日：2006-09-07
申请号：US11431030
申请日：2006-05-10
申请人： Sudhir Sinha , Dale Hedges , Mark Batzer
发明人： Sudhir Sinha , Dale Hedges , Mark Batzer
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6876 , C12Q1/6879 , C12Q2600/156 , Y10S435/975
摘要： A method for determining gender from a human DNA sample. The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment. The gender assay utilizes AluSTXa for the X chromosome, AluSTYa for the Y chromosome, or both AluSTXa and AluSTYa, to reduce the possibility of error to a negligible quantity. The inserted chromosome yields a large fragment when the homologous region is amplified. The males are distinguished as having two DNA amplicons present, while females have only a single amplicon. The kit adapted for carrying out the method includes a pair of primers to amplify the locus and optionally polymerase chain reaction regents.
摘要翻译：一种从人类DNA样本中确定性别的方法。根据片段的大小选择，扩增和评估Alu元件插入的位点。性别测定使用AluSTXa作为X染色体，AluSTYa为Y染色体，或AluSTXa和AluSTYa两者，以将误差的可能性降低到可忽略的数量。当扩增同源区时，插入的染色体产生大片段。男性的区别在于存在两个DNA扩增子，而女性只有一个扩增子。用于实施该方法的试剂盒包括一对扩增基因座和任选的聚合酶链反应试剂的引物。

7. 发明申请

US20050069903A1 Assay for human DNA for gender determination 失效
标题翻译：测定人类DNA进行性别测定
公开(公告)号：US20050069903A1
公开(公告)日：2005-03-31
申请号：US10673854
申请日：2003-09-30
申请人： Sudhir Sinha , Dale Hedges , Mark Batzer
发明人： Sudhir Sinha , Dale Hedges , Mark Batzer
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6879 , C12Q1/6876 , C12Q2600/156
摘要： A method for determining gender from a human DNA sample. The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment. The gender assay utilizes AluSTXα for the X chromosome, AluSTYα for the Y chromosome, or both AluSTXα and AluSTYα, to reduce the possibility of error to a negligible quantity. The inserted chromosome yields a large fragment when the homologous region is amplified. The males are distinguished as having two DNA amplicons present, while females have only a single amplicon. The kit adapted for carrying out the method includes a pair of primers to amplifiy the locus and optionally polymerase chain reaction regents.
摘要翻译：一种从人类DNA样本中确定性别的方法。根据片段的大小选择，扩增和评估Alu元件插入的位点。性别测定使用AluSTXalpha作为X染色体，AluSTYalpha用于Y染色体，或同时使用AluSTXalpha和AluSTYalpha，以将误差的可能性降低到可忽略的数量。当扩增同源区时，插入的染色体产生大片段。男性的区别在于存在两个DNA扩增子，而女性只有一个扩增子。用于实施该方法的试剂盒包括一对扩增基因座和任选的聚合酶链反应试剂的引物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式