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    • 6. 发明申请
    • Device for printing biomolecules on substrate using electrohydrodynamic effect
    • 使用电动力学效应在基板上印刷生物分子的装置
    • US20060255267A1
    • 2006-11-16
    • US11418602
    • 2006-05-05
    • Jeong-gun LeeJeong-woo ChoiNam HuhHye-jung Cho
    • Jeong-gun LeeJeong-woo ChoiNam HuhHye-jung Cho
    • G21K7/00
    • B01L3/0244B01J2219/0036B01J2219/00378B01J2219/00659B01J2219/00722B01J2219/00725B01J2219/00729B01L3/0241B01L2300/0819
    • Provided is a device for printing a biomolecule solution onto a substrate using an electrohydrodynamic (EHD) effect. The device can deposit drops of the biomolecule solution with small size and volume and print more of the biomolecule solution onto a substrate, thereby enabling the manufacture of a high density biochip. The device includes a first electric field forming electrode that is shaped like a needle, is made of a conductive material, is disposed vertically, and includes an accommodating area in which the biomolecule solution is accommodated and an outlet formed on a bottom end of the accommodating area through which the biomolecule solution is discharged; a substrate disposed below the first electric field forming electrode, and including a target surface onto which the biomolecule solution discharged from the outlet of the first electric field forming electrode is deposited; a second electric field forming electrode made of a conductive material and disposed below the first electric field forming electrode; and a voltage applying device which is electrically connected to the first and second electric field forming electrodes to apply a voltage between the first and second electric field forming electrodes so that an electric field is generated around the biomolecule solution suspended in the outlet, wherein, due to the interaction between the electric field and a difference between dielectric constants of the biomolecule solution having a free surface and the surrounding atmosphere, the electric force acts inward on the biomolecule solution, thereby causing a predetermined amount of the biomolecule solution to drop onto the target surface of the substrate.
    • 提供了使用电动力学(EHD)效应将生物分子溶液印刷到基底上的装置。 该装置可以沉积小尺寸和体积的生物分子溶液,并将更多的生物分子溶液印刷到基底上,从而能够制造高密度生物芯片。 该装置包括成形为针状的第一电场形成电极,由导电材料制成,垂直设置,并且包括其中容纳有生物分子溶液的容纳区域和形成在容纳物的底端的出口 生物分子溶液排出的区域; 设置在所述第一电场形成电极下方的基板,并且包括从所述第一电场形成用电极的出口排出的生物分子溶液沉积在其上的目标表面; 由导电材料制成并设置在第一电场形成电极下方的第二电场形成电极; 以及电压施加装置,其电连接到第一和第二电场形成电极,以在第一和第二电场形成电极之间施加电压,使得在悬浮在出口中的生物分子溶液周围产生电场,其中,由于 对于电场与具有自由表面和周围大气的生物分子溶液的介电常数之间的差异的相互作用,电力向内作用于生物分子溶液,从而使预定量的生物分子溶液落到目标上 基板的表面。
    • 7. 发明申请
    • Quantitative cell dispensing apparatus using liquid drop manipulation
    • 使用液滴操作的定量细胞分配装置
    • US20070148763A1
    • 2007-06-28
    • US11491825
    • 2006-07-24
    • Nam HuhJeong-gun Lee
    • Nam HuhJeong-gun Lee
    • C12M3/00
    • B01L3/0241B01L2200/0647B01L2200/0673B01L2200/143B01L2300/0819B01L2300/089B01L2400/027B01L2400/0427G01N2035/00237Y10T436/2575
    • Provided is a quantitative cell dispensing apparatus using liquid droplet manipulation, for quantitatively dispensing cells from hydrophilic suspension containing a plurality of cells. The quantitative cell dispensing apparatus includes hydrophilic suspension, a liquid droplet manipulation unit, a dispensing unit, a sensing unit, and a control unit. The hydrophilic suspension includes a plurality of cells and electrolyte, and the liquid droplet manipulation unit. The liquid droplet manipulation unit includes an inlet and a liquid droplet outlet portion. The dispensing unit discharges a liquid droplet received from the liquid droplet outlet portion to a target portion. The sensing unit optically senses liquid droplets distributed on a panel of the liquid droplet manipulation unit. The control unit analyses the number of cells contained in each liquid droplet using a single sensed by the sensing unit, and controls the liquid droplet manipulation unit to transfer a liquid droplet containing a predetermined number of cells to the liquid droplet outlet portion.
    • 提供了一种使用液滴操作的定量细胞分配装置,用于从含有多个细胞的亲水性悬浮液定量分配细胞。 定量细胞分配装置包括亲水性悬浮液,液滴操作单元,分配单元,感测单元和控制单元。 亲水性悬浮液包括多个细胞和电解质,以及液滴操作单元。 液滴操作单元包括入口和液滴出口部分。 分配单元将从液滴出口部分接收的液滴排放到目标部分。 感测单元光学地感测分布在液滴操作单元的面板上的液滴。 控制单元使用感测单元检测到的单个分析每个液滴中包含的细胞的数量,并且控制液滴操作单元将含有预定数量的细胞的液滴转移到液滴出口部分。
    • 8. 发明授权
    • Quantitative cell dispensing apparatus using liquid drop manipulation
    • 使用液滴操作的定量细胞分配装置
    • US07901633B2
    • 2011-03-08
    • US11491825
    • 2006-07-24
    • Nam HuhJeong-gun Lee
    • Nam HuhJeong-gun Lee
    • B01L3/02G01N1/10
    • B01L3/0241B01L2200/0647B01L2200/0673B01L2200/143B01L2300/0819B01L2300/089B01L2400/027B01L2400/0427G01N2035/00237Y10T436/2575
    • Provided is a quantitative cell dispensing apparatus using liquid droplet manipulation, for quantitatively dispensing cells from hydrophilic suspension containing a plurality of cells. The quantitative cell dispensing apparatus includes hydrophilic suspension, a liquid droplet manipulation unit, a dispensing unit, a sensing unit, and a control unit. The hydrophilic suspension includes a plurality of cells and electrolyte, and the liquid droplet manipulation unit. The liquid droplet manipulation unit includes an inlet and a liquid droplet outlet portion. The dispensing unit discharges a liquid droplet received from the liquid droplet outlet portion to a target portion. The sensing unit optically senses liquid droplets distributed on a panel of the liquid droplet manipulation unit. The control unit analyses the number of cells contained in each liquid droplet using a single sensed by the sensing unit, and controls the liquid droplet manipulation unit to transfer a liquid droplet containing a predetermined number of cells to the liquid droplet outlet portion.
    • 提供了一种使用液滴操作的定量细胞分配装置,用于从含有多个细胞的亲水性悬浮液定量分配细胞。 定量细胞分配装置包括亲水性悬浮液,液滴操作单元,分配单元,感测单元和控制单元。 亲水性悬浮液包括多个细胞和电解质,以及液滴操作单元。 液滴操作单元包括入口和液滴出口部分。 分配单元将从液滴出口部分接收的液滴排放到目标部分。 感测单元光学地感测分布在液滴操作单元的面板上的液滴。 控制单元使用感测单元检测到的单个分析每个液滴中包含的细胞的数量,并且控制液滴操作单元将含有预定数量的细胞的液滴转移到液滴出口部分。
    • 10. 发明申请
    • Dual Clutch Transmission And Dual Clutch Accuators Thereof
    • 双离合器变速器和双离合器调速器
    • US20120090428A1
    • 2012-04-19
    • US12903617
    • 2010-10-13
    • Thomas C. BowenNam Huh
    • Thomas C. BowenNam Huh
    • F16H37/06F16D21/00
    • F16H3/08F16D21/06F16D23/12F16D2021/0646F16D2021/0692F16D2023/141F16H3/006F16H2003/0931
    • A dual clutch transmission with multiple gears operatively coupled thereto, comprises: first and second input shafts coaxially arranged to each other; a first clutch operatively coupled with the first input shaft to drive the transmission with a gear selected from a first set of non-consecutively numbered gears; a second clutch coaxially arranged with the first clutch and operatively coupled with the second input shaft to drive the transmission with a gear selected from a second set of non-consecutively numbered gears; first and second clutch actuating devices, each including an actuator and a pivot member, each pivot member operatively coupled between the actuator and a clutch applicator of cylindrical shape to move its clutch applicator for clutch operation; and a plurality of first and second clutch levers received in a clutch retaining housing in a circularly and alternately arranged pattern. The first and second clutch levers are configured to move the first and second clutches, respectively. The cylindrically shaped clutch applicator of the first clutch actuating device is disposed coaxially with the cylindrically shaped clutch applicator of the second clutch actuating device, and the first clutch levers and the second clutch levers can operate independently to each other upon movement of the first clutch applicator or the second clutch applicator to perform a selective clutch operation between the first and second clutches.
    • 具有与其可操作地连接的多个齿轮的双离合器变速器包括:彼此同轴布置的第一和第二输入轴; 第一离合器,其与第一输入轴可操作地联接,以用选自第一组非连续编号的齿轮的档位来驱动变速器; 与所述第一离合器同轴地布置的第二离合器,并且与所述第二输入轴可操作地联接以利用选自第二组非连续编号的齿轮的档位驱动所述变速器; 第一和第二离合器致动装置,每个包括致动器和枢转构件,每个枢转构件可操作地联接在致动器和圆柱形形式的离合器施加器之间,以移动其离合器施加器用于离合器操作; 多个第一和第二离合器杆以圆形和交替排列的图案容纳在离合器保持壳体中。 第一和第二离合器杆分别构造成移动第一和第二离合器。 第一离合器致动装置的圆柱形离合器施加器与第二离合器致动装置的圆柱形离合器施加器同轴设置,并且当第一离合器施加器运动时第一离合器杆和第二离合器杆可以彼此独立地操作 或第二离合器施加器,以在第一和第二离合器之间执行选择性离合器操作。