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1. 发明授权

US06991741B2 Controlled room temperature synthesis of magnetic metal oxide nanoclusters within a diblock copolymer matrix 失效
标题翻译：二嵌段共聚物基体内磁性金属氧化物纳米团簇的室温合成
公开(公告)号：US06991741B2
公开(公告)日：2006-01-31
申请号：US10250433
申请日：2002-11-29
申请人： Steven Bullock , Sufi Rizwan Ahmed , Peter Kofinas
发明人： Steven Bullock , Sufi Rizwan Ahmed , Peter Kofinas
IPC分类号： H01F1/37
CPC分类号： B82Y25/00 , H01F1/0027 , H01F1/0063 , H01F1/37 , H01F41/16
摘要： A method of room temperature synthesis of magnetic metal oxide nanoclusters within a diblock copolymer matrix includes the step of synthesizing, by ring opening metathesis polymerization technique, a diblock copolymer having a repeat unit ratio m/n, introducing, at room temperature, one or several metal containing precursors into the one block of the diblock copolymer, and processing the metal containing diblock copolymer by wet chemical technique to form nanoclusters of the metal(s) oxide within the diblock copolymer matrix. Specific reaction for synthesis of CoFe3O4 and Co3O4 nanoclusters within diblock copolymers, such as [NOR]m/[NORCOOH]n and [NOR]m/[CO(bTAN)]n, respectively is used in the method of the present invention.
摘要翻译：在二嵌段共聚物基质中室温合成磁性金属氧化物纳米团簇的方法包括通过开环复分解聚合技术合成具有重复单元比率m / n的二嵌段共聚物的步骤，在室温下引入一个或几个将含金属的前体引入二嵌段共聚物的一个嵌段中，并通过湿法化学技术处理含金属的二嵌段共聚物，以形成二嵌段共聚物基质内的氧化金属的纳米团簇。用于合成二嵌段共聚物内的CoFe 3 O 4 O 4和Co 3 O 4 O 4纳米团簇的具体反应，例如[ n N]]> [NORCOOH] n和/或[NOR] m / [CO（bTAN）] n < 分别用于本发明的方法。

2. 发明授权

US07771955B2 Affinity membrane for capture of a target biomolecule and formation thereof by site-directed immobilization of a capture biomolecule 有权
标题翻译：用于捕获靶生物分子的亲和膜及其通过捕获生物分子的定点固定形成
公开(公告)号：US07771955B2
公开(公告)日：2010-08-10
申请号：US11447154
申请日：2006-06-06
申请人： Timothy Alan Barbari , Sufi Rizwan Ahmed
发明人： Timothy Alan Barbari , Sufi Rizwan Ahmed
IPC分类号： G01N33/53 , C07F15/04 , C12P21/02
CPC分类号： C07K17/06 , G01N33/54353 , G01N33/545
摘要： Compositions and methods are taught for directing the orientation of an immobilized capture biomolecule on a hydrophobic membrane. The method comprises layering at least one tie layer on a hydrophobic membrane, adding an amine functional layer on top of at least one tie layer; and attaching an alignment biomolecule to the amine functional layer. The alignment biomolecule has the ability to either capture a target biomolecule itself and thus be considered a capture biomolecule, or bind and orient the immobilized capture biomolecule so as to maximize the binding activity of the immobilized capture biomolecule. In one embodiment, a nickel-coordinated amine functional layer binds with a histidine-tagged alignment biomolecule. In another embodiment, an amine functional layer reacts, via tyrosinase catalysis, with a tyrosine residue in an alignment biomolecule.
摘要翻译：教导组合物和方法将固定的捕获生物分子的取向引导到疏水膜上。该方法包括在疏水膜上层叠至少一个粘结层，在至少一个粘结层的顶部上加入胺官能层; 并将取向生物分子附着到胺官能层上。取向生物分子具有捕获目标生物分子本身的能力，因此被认为是捕获生物分子，或者结合和定向固定的捕获生物分子，以便使固定的捕获生物分子的结合活性最大化。在一个实施方案中，镍配位的胺功能层与组氨酸标记的取向生物分子结合。在另一个实施方案中，胺功能层通过酪氨酸酶催化与对准生物分子中的酪氨酸残基反应。

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