专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US09637783B1 Amplification of oligonucleotides containing non-standard nucleotides by polymerase chain reactions 有权
公开(公告)号：US09637783B1
公开(公告)日：2017-05-02
申请号：US14746243
申请日：2015-06-22
申请人： Steven Benner , Roberto Laos , Nicole Leal , Nilesh Karalkar , Zunyi Yang
发明人： Steven Benner , Roberto Laos , Nicole Leal , Nilesh Karalkar , Zunyi Yang
IPC分类号： C12Q1/68 , C12P19/34 , C12N9/12
CPC分类号： C12Q1/6869 , C12N9/1252 , C12P19/34 , C12Q1/6844 , C12Q2525/117 , C12Y207/07007 , C12Q2525/101
摘要： This invention relates to processes that amplify, in a polymerase chain reaction architecture, oligonucleotide analogs that incorporate non-standard nucleobase analogs from an artificially expanded genetic information system. These pair in DNA duplexes via patterns of hydrogen bonds that are different from patterns that join the thymine-adenine and guanine-cytosine nucleobase pairs.

2. 发明授权

US09862983B1 Recombinase-based amplification with substitute nucleotides 有权
公开(公告)号：US09862983B1
公开(公告)日：2018-01-09
申请号：US14746293
申请日：2015-06-22
申请人： Steven Benner , Nilesh Karalkar
发明人： Steven Benner , Nilesh Karalkar
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q1/6853 , C12Q2521/507 , C12Q2525/101 , C12Q2537/157
摘要： This invention covers methods for isothermal amplification of DNA, based on the unexpected discovery that primers having, at some positions, adenine substituted by 2-aminopurine or diaminopurine, guanine substituted by inosine, thymine substituted by 2-thiothymine, and cytosine substituted by N4-ethylcytosine are accepted by enzymes used in standard recombinase polymerase assays (RPA). Further unexpected was the discovery that target nucleotides are efficiently amplified in an RPA-like process (hereinafter abbreviated as simply RPA) using substituted primers. The invention also covers RPA-like processes that use substituted primers tagged with oligonucleotides incorporating nucleotides from artificially expanded genetic information systems (AEGIS).

3. 发明申请

US20050038609A1 Evolution-based functional genomics 审中-公开
标题翻译：基于进化的功能基因组学
公开(公告)号：US20050038609A1
公开(公告)日：2005-02-17
申请号：US10765120
申请日：2004-01-28
申请人： Steven Benner
发明人： Steven Benner
IPC分类号： G01N33/68 , G06F19/00 , G01N33/48 , G01N33/50
CPC分类号： G01N33/6803
摘要： The invention concerns methods for applying evolutionary analyses to a set of aligned homologous protein sequences for the purpose of predicting a consensus model for the folded secondary structure of a protein family, identifying distant homologs and denying distant homology, assigning functional behavior to protein families, identifying protein pairs that interact as they function, identifying episodes of sequence evolution where functional behavior within a family is changing, and identifying specific chemical units of the protein that change in concert with changes in functional behavior. Accordingly, this invention is relevant to the use of genomic information to understand homology, fold, behavior and function in proteins.
摘要翻译：本发明涉及将进化分析应用于一组对准的同源蛋白质序列的方法，目的是预测蛋白质家族的折叠二级结构的共有模型，识别远距离同源物并拒绝远距离同源性，为蛋白质家族分配功能行为，鉴定在功能上相互作用的蛋白质对，识别家族中功能行为发生变化的序列进化情况，以及鉴定与功能行为变化相一致变化的蛋白质的特定化学单位。因此，本发明与使用基因组信息来了解蛋白质的同源性，折叠，行为和功能相关。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式