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1. 发明授权

US08685677B2 Detection of AAD-12 soybean event 416 有权
标题翻译： AAD-12大豆事件的检测416
公开(公告)号：US08685677B2
公开(公告)日：2014-04-01
申请号：US13511995
申请日：2010-11-24
申请人： Stephen Novak , Yunxing C. Cui , Thomas W. Greene , Ning Zhou
发明人： Stephen Novak , Yunxing C. Cui , Thomas W. Greene , Ning Zhou
IPC分类号： C12P19/34
CPC分类号： C12N15/8274 , A01H1/04 , C12Q1/6895 , C12Q2600/16 , C12Q2600/166
摘要： The invention relates in part to methods of detecting an AAD-12 soybean event. The subject invention provides assays for detecting the presence of the subject event in a sample (of soybeans, for example). Kits and conditions useful in conducting the assays are also provided. More specifically, the present invention relates in part to an endpoint TaqMan PCR assay for the AAD-12 soybean event. Some embodiments are directed to assays that are capable of high throughput zygosity analysis. The subject invention further relates, in part, to the discovery of a preferred reference gene for use in determining zygosity. This invention also relates in part to plant breeding using any of the subject methods. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits. The subject procedures can be used to uniquely identify soybean lines comprising the event of the subject invention.
摘要翻译：本发明部分涉及AAD-12大豆事件的检测方法。本发明提供了用于检测样品（例如大豆）的受试者事件的存在的测定法。还提供了用于进行测定的试剂盒和条件。更具体地，本发明部分涉及AAD-12大豆事件的终点TaqMan PCR测定。一些实施方案涉及能够进行高通量接合度分析的测定。本发明还部分涉及用于确定接合性的优选参考基因的发现。本发明还部分地涉及使用任何主题方法的植物育种。在一些实施方案中，所述事件/多核苷酸序列可以与其他特征“堆叠”。本发明方法可用于唯一地鉴定包含本发明事件的大豆品系。

2. 发明申请

US20120244533A1 DETECTION OF AAD1 EVENT DAS-40278-9 有权
标题翻译：检测AAD1事件DAS-40278-9
公开(公告)号：US20120244533A1
公开(公告)日：2012-09-27
申请号：US13390979
申请日：2010-08-18
申请人： Ning Zhou , Stephen Novak , Yunxing C. Cui , Thomas W. Greene
发明人： Ning Zhou , Stephen Novak , Yunxing C. Cui , Thomas W. Greene
IPC分类号： G01N21/64
CPC分类号： A01H1/04 , C12N15/8274 , C12Q1/6895 , C12Q2561/101 , C12Q2600/13
摘要： This invention relates in part to detecting herbicide tolerant plants—more specifically, an aad-1 transformation event in corn plants. The subject invention also provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). Kits and conditions useful in conducting the assays are also provided. The subject invention also relates in part to plant breeding using the subject methods. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits. More specifically, the invention relates in part to an endpoint TaqMan PCR assay for AAD-1 corn event 40278-9. Some embodiments are directed to assays that are capable of high throughput zygosity analysis. The subject invention further relates, in part, to the use of a preferred reference gene for use in determining zygosity.
摘要翻译：本发明部分地涉及检测除草剂耐受性植物 - 更具体地，涉及玉米植物中的aad-1转化事件。本发明还提供了用于检测样品（例如玉米粒）中的受试者事件的存在的测定法。还提供了用于进行测定的试剂盒和条件。本发明也部分地涉及使用本发明方法的植物育种。在一些实施方案中，所述事件/多核苷酸序列可以与其他特征“堆叠”。更具体地，本发明部分涉及AAD-1玉米事件40278-9的终点TaqMan PCR测定。一些实施方案涉及能够进行高通量接合度分析的测定。本发明进一步涉及用于确定接合性的优选参考基因的用途。

3. 发明授权

US09738904B2 Insect resistant and herbicide tolerant breeding stack of soybean event pDAB9582.814.19.1 and pDAB4468.04.16.1 有权
公开(公告)号：US09738904B2
公开(公告)日：2017-08-22
申请号：US14234923
申请日：2012-07-26
申请人： Yunxing C. Cui , Thomas Hoffman , Dawn M. Parkhurst , Ning Zhou , Barry Wiggins , Dayakar Pareddy , Gregory A. Bradfisch , James E. Dripps , Sandra G. Toledo , Nathan Bard , Michael Vercauteren , Nandi Nagaraj , Brandon Bishop , Gregory J. Gilles , Terry R. Wright , Julissa Colon , Ricardo A. Barnes , Nathan J. VanOpdorp , Yonghe Bai
发明人： Yunxing C. Cui , Thomas Hoffman , Dawn M. Parkhurst , Ning Zhou , Barry Wiggins , Dayakar Pareddy , Gregory A. Bradfisch , James E. Dripps , Sandra G. Toledo , Nathan Bard , Michael Vercauteren , Nandi Nagaraj , Brandon Bishop , Gregory J. Gilles , Terry R. Wright , Julissa Colon , Ricardo A. Barnes , Nathan J. VanOpdorp , Yonghe Bai
IPC分类号： A01H1/00 , A01H5/00 , A01H5/10 , C12N5/04 , C07H21/04 , C12N15/82 , C12Q1/68 , A01N37/38 , A01N37/44 , A01N65/20
CPC分类号： C12N15/8286 , A01N37/38 , A01N37/44 , A01N65/20 , C12N15/8209 , C12N15/8274 , C12N15/8277 , C12Q1/6895 , C12Q2600/13 , Y02A40/162
摘要： A breeding stack of soybean event 9582.814.19.1 and soybean event pDAB4468.04.16.1 resulted in the novel soybean event pDAB9582.814.19.1::pDAB4468.04.16.1. Soybean event pDAB9582.814.19.1::pDAB4468.04.16.1 comprises genes encoding AAD-12, Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products.

4. 发明申请

US20120222153A1 AAD-1 EVENT DAS-40278-9, RELATED TRANSGENIC CORN LINES, AND EVENT-SPECIFIC IDENTIFICATION THEREOF 有权
标题翻译： AAD-1事件DAS-40278-9，相关转基因线及其事件特异性鉴定
公开(公告)号：US20120222153A1
公开(公告)日：2012-08-30
申请号：US13390969
申请日：2010-08-18
申请人： Yunxing C. Cui , Jill R. Bryan , Donald G. Maum , Gregory J. Gilles , Terry R. Wright , Jennifer L. Hamilton , Nicole L. Arnold , Nathan J. VanOpdorp , Tina M. Kaiser , Ning Zhou
发明人： Yunxing C. Cui , Jill R. Bryan , Donald G. Maum , Gregory J. Gilles , Terry R. Wright , Jennifer L. Hamilton , Nicole L. Arnold , Nathan J. VanOpdorp , Tina M. Kaiser , Ning Zhou
IPC分类号： A01H5/00 , C12N15/82 , A01H5/02 , A01H1/02 , A01H5/06 , A01H5/12 , C12N15/29 , C12Q1/68 , A01H5/10 , A01H5/04
CPC分类号： A01H5/10 , C12N9/0069 , C12N9/0071 , C12N15/8213 , C12N15/8274 , C12Q1/6895 , C12Q2600/16 , C12Y113/00
摘要： This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.
摘要翻译：本发明部分涉及植物育种和除草剂耐受性植物。本发明包括在玉米植物中的新型aad-1转化事件，其包含插入到玉米细胞基因组内特定位点的本文所述的多核苷酸序列。在一些实施方案中，所述事件/多核苷酸序列可以与其他性状“堆叠”，包括例如其它除草剂耐受基因和/或昆虫抑制蛋白。另外，本发明提供了用于检测样品中（例如玉米粒）的受试者事件的存在的测定法。测定可以基于重组构建体的DNA序列，插入到玉米基因组中，并且基于插入位点侧翼的基因组序列。还提供了用于进行测定的试剂盒和条件。

5. 发明授权

US09944944B2 AAD-12 event 416, related transgenic soybean lines, and event-specific identification thereof 有权
公开(公告)号：US09944944B2
公开(公告)日：2018-04-17
申请号：US13511990
申请日：2010-11-24
申请人： Yunxing C. Cui , Thomas Hoffman , Ning Zhou , Gregory J. Gilles , Terry R. Wright , Julissa Colon , Ricardo A. Barnes , Nathan J. VanOpdorp , Yonghe Bai
发明人： Yunxing C. Cui , Thomas Hoffman , Ning Zhou , Gregory J. Gilles , Terry R. Wright , Julissa Colon , Ricardo A. Barnes , Nathan J. VanOpdorp , Yonghe Bai
IPC分类号： C12N15/82 , A01H1/02 , A01H5/10 , A01H1/04 , C12N9/02 , C12Q1/68
CPC分类号： C12N15/8274 , A01H1/02 , A01H1/04 , A01H5/10 , C12N9/0069 , C12N9/0071 , C12Q1/6895 , C12Q2600/156
摘要： This invention includes a novel aad-12 transformation event for herbicide tolerance in soybean plants—referred to herein as pDAB4468-0416. This invention includes a heterologous polynucleotide inserted into a specific site within the genome of a soybean cell. In some embodiments, said event/polynucleotide can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (a soybean, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the soybean genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.

6. 发明授权

US09402358B2 AAD-1 event DAS-40278-9, related transgenic corn lines, and event-specific identification thereof 有权
标题翻译： AAD-1事件DAS-40278-9，相关转基因玉米品系及其事件特异性鉴定
公开(公告)号：US09402358B2
公开(公告)日：2016-08-02
申请号：US13390969
申请日：2010-08-18
申请人： Yunxing C. Cui , Jill R. Bryan , Donald G. Maum , Gregory J. Gilles , Terry R. Wright , Jennifer L. Hamilton , Nicole L. Arnold , Nathan J. VanOpdorp , Tina M. Kaiser , Ning Zhou
发明人： Yunxing C. Cui , Jill R. Bryan , Donald G. Maum , Gregory J. Gilles , Terry R. Wright , Jennifer L. Hamilton , Nicole L. Arnold , Nathan J. VanOpdorp , Tina M. Kaiser , Ning Zhou
IPC分类号： C07H21/04 , C12Q1/68 , C12N5/00 , A01H5/10 , C12N9/02 , C12N15/82
CPC分类号： A01H5/10 , C12N9/0069 , C12N9/0071 , C12N15/8213 , C12N15/8274 , C12Q1/6895 , C12Q2600/16 , C12Y113/00
摘要： This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.
摘要翻译：本发明部分涉及植物育种和除草剂耐受性植物。本发明包括在玉米植物中的新型aad-1转化事件，其包含插入到玉米细胞基因组内特定位点的本文所述的多核苷酸序列。在一些实施方案中，所述事件/多核苷酸序列可以与其他性状“堆叠”，包括例如其它除草剂耐受基因和/或昆虫抑制蛋白。另外，本发明提供了用于检测样品中（例如玉米粒）的受试者事件的存在的测定法。测定可以基于重组构建体的DNA序列，插入到玉米基因组中，并且基于插入位点侧翼的基因组序列。还提供了用于进行测定的试剂盒和条件。

7. 发明申请

US20130324408A1 AAD-12 EVENT 416, RELATED TRANSGENIC SOYBEAN LINES, AND EVENT-SPECIFIC IDENTIFICATION THEREOF 有权
标题翻译： AAD-12事件416，相关转基因大豆品种及其事件特异性鉴定
公开(公告)号：US20130324408A1
公开(公告)日：2013-12-05
申请号：US13511990
申请日：2010-11-24
申请人： Yunxing C. Cui , Thomas Hoffman , Ning Zhou , Greg J. Gilles , Terry R. Wright , Julissa Colon , Ricardo A. Barns , Nathan J. VanOpdrop , Yonghe Bai
发明人： Yunxing C. Cui , Thomas Hoffman , Ning Zhou , Greg J. Gilles , Terry R. Wright , Julissa Colon , Ricardo A. Barns , Nathan J. VanOpdrop , Yonghe Bai
IPC分类号： C12N15/82 , C12Q1/68 , C12N9/02
CPC分类号： C12N15/8274 , A01H1/02 , A01H1/04 , A01H5/10 , C12N9/0069 , C12N9/0071 , C12Q1/6895 , C12Q2600/156
摘要： This invention includes a novel aad-12 transformation event for herbicide tolerance in soybean plants—referred to herein as pDAB4468-0416. This invention includes a heterologous polynucleotide inserted into a specific site within the genome of a soybean cell. In some embodiments, said event/polynucleotide can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (a soybean, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the soybean genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.
摘要翻译：本发明包括大豆植物中除草剂耐受性的新型aad-12转化事件 - 本文称为pDAB4468-0416。本发明包括插入大豆细胞基因组内特定位点的异源多核苷酸。在一些实施方案中，所述事件/多核苷酸可以与其它性状“堆叠”，包括例如其它除草剂耐受性基因和/或昆虫抑制性蛋白质。另外，本发明提供了用于检测样品（例如大豆）中受试者事件的存在的测定法。测定可以基于重组构建体的DNA序列，插入大豆基因组中，以及插入位点侧翼的基因组序列。还提供了用于进行测定的试剂盒和条件。

8. 发明授权

US08680363B2 Insect resistant and herbicide tolerant soybean event 9582.814.19.1 有权
标题翻译：抗虫和除草剂耐受性大豆事件9582.814.19.1
公开(公告)号：US08680363B2
公开(公告)日：2014-03-25
申请号：US13559177
申请日：2012-07-26
申请人： Nathan Bard , Gregory A. Bradfisch , Yunxing C. Cui , James E. Dripps , Thomas Hoffman , Dayakar Pareddy , Dawn M. Parkhurst , Sandra G. Toledo , Barry Wiggins , Ning Zhou
发明人： Nathan Bard , Gregory A. Bradfisch , Yunxing C. Cui , James E. Dripps , Thomas Hoffman , Dayakar Pareddy , Dawn M. Parkhurst , Sandra G. Toledo , Barry Wiggins , Ning Zhou
IPC分类号： C12N15/82 , A01H1/00 , A01H5/10 , A01H5/00
CPC分类号： C12N15/8286 , A01N37/38 , A01N37/44 , A01N65/20 , C12N15/8209 , C12N15/8274 , C12N15/8277 , C12Q1/6895 , C12Q2600/13 , Y02A40/162
摘要： Soybean event 9582.814.19.1, wherein the event comprises genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products.
摘要翻译：大豆事件9582.814.19.1，其中事件包括编码Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及用于作物保护和储存产品保护的方法。

9. 发明申请

US20140344994A1 INSECT RESISTANT AND HERBICIDE TOLERANT BREEDING STACK OF SOYBEAN EVENT PDAB9582.814.19.1 AND PDAB4468.04.16.1 有权
标题翻译：大豆活性耐药性和抗除草剂耐受性堆肥PDAB9582.814.19.1和PDAB4468.04.16.1
公开(公告)号：US20140344994A1
公开(公告)日：2014-11-20
申请号：US14234923
申请日：2012-07-26
申请人： Yunxing C. Cui , Thomas Hoffman , Dawn M. Parkhurst , Ning Zhou , Barry Wiggins , Dayakar Pareddy , Gregory A. Bradfisch , James E. Dripps , Sandra G. Toledo , Nathan Bard , Michael Vercauteren , Nandi Nagaraj , Brandon Bishop , Gregory J. Gilles , Terry R. Wright , Julissa Colon , Ricardo A. Barnes , Nathan J. VanOpdorp , Yonghe Bai
发明人： Yunxing C. Cui , Thomas Hoffman , Dawn M. Parkhurst , Ning Zhou , Barry Wiggins , Dayakar Pareddy , Gregory A. Bradfisch , James E. Dripps , Sandra G. Toledo , Nathan Bard , Michael Vercauteren , Nandi Nagaraj , Brandon Bishop , Gregory J. Gilles , Terry R. Wright , Julissa Colon , Ricardo A. Barnes , Nathan J. VanOpdorp , Yonghe Bai
IPC分类号： C12N15/82 , A01N65/20 , A01N37/38 , A01N37/44
CPC分类号： C12N15/8286 , A01N37/38 , A01N37/44 , A01N65/20 , C12N15/8209 , C12N15/8274 , C12N15/8277 , C12Q1/6895 , C12Q2600/13 , Y02A40/162
摘要： A breeding stack of soybean event 9582.814.19.1 and soybean event pDAB4468.04.16.1 resulted in the novel soybean event pDAB9582.814.19.1 :: pDAB4468.04.16.1. Soybean event pDAB9582.814.19.1 :: pDAB4468.04.16.1 comprises genes encoding AAD-12, CrylF, CrylAc (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products.
摘要翻译：大豆事件9582.814.19.1和大豆事件pDAB4468.04.16.1的育种堆栈导致了大豆事件pDAB9582.814.19.1 :: pDAB4468.04.16.1。大豆事件pDAB9582.814.19.1 :: pDAB4468.04.16.1包含编码AAD-12，Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及有效的作物保护方法并保护储存的产品。

10. 发明授权

US08785728B2 AAD-12 event 1606 and related transgenic soybean lines 有权
标题翻译： AAD-12事件1606和相关的转基因大豆品系
公开(公告)号：US08785728B2
公开(公告)日：2014-07-22
申请号：US13226789
申请日：2011-09-07
申请人： Yunxing C. Cui , Thomas Hoffman , Ning Zhou , Gregory J. Gilles , Terry R. Wright , Dawn M. Parkhurst , Julissa Colon , Yonghe Bai
发明人： Yunxing C. Cui , Thomas Hoffman , Ning Zhou , Gregory J. Gilles , Terry R. Wright , Dawn M. Parkhurst , Julissa Colon , Yonghe Bai
IPC分类号： C12N15/82 , A01H1/00 , C07H21/04
CPC分类号： C12N15/8274 , A01H1/04 , C12N9/0004
摘要： This invention relates to soybean event pDAB4472-1606 (Event 1606). This invention includes a novel aad-12 transformation event in soybean plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a soybean cell. This invention also relates in part to plant breeding and herbicide tolerant plants. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins.
摘要翻译：本发明涉及大豆事件pDAB4472-1606（事件1606）。本发明包括大豆植物中的新型aad-12转化事件，其包含插入大豆细胞基因组内的特定位点的本文所述的多核苷酸序列。本发明还部分涉及植物育种和除草剂耐受性植物。在一些实施方案中，所述事件/多核苷酸序列可以与其他性状“堆叠”，包括例如其它除草剂耐受基因和/或昆虫抑制蛋白。

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