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1. 发明申请

US20100317050A1 POLYPEPTIDES FOR MICROBIAL DETECTION 有权
标题翻译：用于微生物检测的多核苷酸
公开(公告)号：US20100317050A1
公开(公告)日：2010-12-16
申请号：US12867078
申请日：2009-02-12
申请人： Stephen B. Roscoe , Stephanie J. Moeller , Yi He , Richard B. Ross
发明人： Stephen B. Roscoe , Stephanie J. Moeller , Yi He , Richard B. Ross
IPC分类号： C12Q1/02 , C07K7/08 , C07K14/00
CPC分类号： G01N33/56961 , C07K7/08 , G01N2469/10
摘要： Polypeptides which can be activated to cause the formation of pores in a lipid membrane are disclosed. Also disclosed are polypeptide compositions for the detection of target microorganisms and methods of using said compositions.
摘要翻译：公开了可以被活化以在脂质膜中形成孔的多肽。还公开了用于检测靶微生物的多肽组合物和使用所述组合物的方法。

2. 发明授权

US08497353B2 Polypeptides for microbial detection 有权
公开(公告)号：US08497353B2
公开(公告)日：2013-07-30
申请号：US12867078
申请日：2009-02-12
申请人： Stephen B. Roscoe , Stephanie J. Moeller , Yi He , Richard B. Ross
发明人： Stephen B. Roscoe , Stephanie J. Moeller , Yi He , Richard B. Ross
IPC分类号： A61K38/16
CPC分类号： G01N33/56961 , C07K7/08 , G01N2469/10
摘要： Polypeptides which can be activated to cause the formation of pores in a lipid membrane are disclosed. Also disclosed are polypeptide compositions for the detection of target microorganisms and methods of using said compositions.

3. 发明申请

US20120301911A1 Rapid Detection of Molds that Produce Glucose Oxidase 有权
标题翻译：快速检测产生葡萄糖氧化酶的模具
公开(公告)号：US20120301911A1
公开(公告)日：2012-11-29
申请号：US13515856
申请日：2010-12-21
申请人： Stephen B. Roscoe , Michael J. Svarovsky , Stephanie J. Moeller
发明人： Stephen B. Roscoe , Michael J. Svarovsky , Stephanie J. Moeller
IPC分类号： G01N21/78
CPC分类号： C12Q1/04 , C12Q1/045 , C12Q1/26 , C12Q1/54
摘要： Methods and kits are disclosed for detecting microorganisms that produce glucose oxidase. The method includes providing a culture medium and a hydrogen peroxide indicating reagent comprising a chromogenic substrate that can provide a detectable chromogenic reaction indicating the presence of a microorganism that produces glucose oxidase, and additional methods are disclosed for differentiating microorganisms by the detection of an additional chromogenic reaction.
摘要翻译：公开了用于检测产生葡萄糖氧化酶的微生物的方法和试剂盒。该方法包括提供培养基和过氧化氢指示试剂，其包含可产生可产生葡萄糖氧化酶的微生物存在的可检测的生色反应的显色底物，并且公开了用于通过检测另外的显色性来分化微生物的另外的方法反应。

4. 发明申请

US20130230876A1 METHODS AND COMPOSITIONS TO DETECT A BIOLOGICAL ACTIVITY 有权
标题翻译：检测生物活性的方法和组合物
公开(公告)号：US20130230876A1
公开(公告)日：2013-09-05
申请号：US13883620
申请日：2011-10-28
申请人： Stephen B. Roscoe , Kurt J. Halverson , Jesse D. Miller , Stephanie J. Moeller , Jason W. Bjork
发明人： Stephen B. Roscoe , Kurt J. Halverson , Jesse D. Miller , Stephanie J. Moeller , Jason W. Bjork
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , C12Q1/04
摘要： Compositions that comprise water, a first indicator reagent that can be converted by a first biological activity to a first biological derivative, and a plurality of particles are provided. The first indicator reagent can comprise a fluorogenic enzyme substrate having a fluorophore selected from the group consisting of umbelliferone, 7-aminocoumarin, β-naphthylamine, β-naphthol, fluorescein, resorufin, 9H-(1,3-dichloro-9,9-dimethyl acridin-2-one), rhodamine 110, a derivative of any of the foregoing fluorophores, and a combination of any of the foregoing fluorophores. The particles are capable of receiving and retaining the first biological derivative from an aqueous liquid. The first biological derivative can be indicative of a microorganism. The compositions further can comprise a gelling agent. Methods of using the compositions to detect the presence or absence of a microorganism in a sample are also provided.
摘要翻译：提供了包含水，可以通过第一生物活性转化为第一生物衍生物的第一指示剂试剂和多个颗粒的组合物。第一指示剂可以包括具有选自伞形酮，7-氨基香豆素，β-萘胺，β-萘酚，荧光素，再次吸收的9H-（1,3-二氯-9,9-四氢 - 二甲基吖啶-2-酮），罗丹明110，任何前述荧光团的衍生物，以及任何上述荧光团的组合。颗粒能够从水性液体中接收和保留第一生物衍生物。第一种生物衍生物可以指示微生物。组合物还可以包含胶凝剂。还提供了使用组合物来检测样品中微生物的存在或不存在的方法。

5. 发明授权

US09434975B2 Fluorogenic or fluorophoric compounds and uses thereof 有权
标题翻译：荧光或荧光化合物及其用途
公开(公告)号：US09434975B2
公开(公告)日：2016-09-06
申请号：US14008905
申请日：2012-03-05
申请人： Stephen B. Roscoe , Shih-Hung Chou , Stephanie J. Moeller , Jesse D. Miller , Kurt J. Halverson , Jason W. Bjork
发明人： Stephen B. Roscoe , Shih-Hung Chou , Stephanie J. Moeller , Jesse D. Miller , Kurt J. Halverson , Jason W. Bjork
IPC分类号： C12Q1/04 , C07D311/16 , C07D405/04 , C07D409/04 , C07H17/075 , G01N33/52 , G01N33/58
CPC分类号： C12Q1/04 , C07D311/16 , C07D405/04 , C07D409/04 , C07H17/075 , C09B57/02 , C12Q1/045 , G01N33/525 , G01N33/582
摘要： Compounds are provided that are either fluorogenic or fluorophoric. Compositions and articles that include the compounds are also provided. Additionally, methods of detecting a microorganism using the compounds are provided. The compounds are fluorinated and can be used advantageously under acidic conditions.
摘要翻译：提供了荧光或荧光的化合物。还提供了包含化合物的组合物和制品。另外，提供使用该化合物检测微生物的方法。化合物是氟化的，并且可以在酸性条件下有利地使用。

6. 发明授权

US08951748B2 Rapid detection of molds that produce glucose oxidase 有权
标题翻译：快速检测产生葡萄糖氧化酶的霉菌
公开(公告)号：US08951748B2
公开(公告)日：2015-02-10
申请号：US13515856
申请日：2010-12-21
申请人： Stephen B. Roscoe , Michael J. Svarovsky , Stephanie J. Moeller
发明人： Stephen B. Roscoe , Michael J. Svarovsky , Stephanie J. Moeller
IPC分类号： G01N33/573 , C12Q1/04 , C12Q1/26 , C12Q1/54
CPC分类号： C12Q1/04 , C12Q1/045 , C12Q1/26 , C12Q1/54
摘要： Methods and kits are disclosed for detecting microorganisms that produce glucose oxidase. The method includes providing a culture medium and a hydrogen peroxide indicating reagent comprising a chromogenic substrate that can provide a detectable chromogenic reaction indicating the presence of a microorganism that produces glucose oxidase, and additional methods are disclosed for differentiating microorganisms by the detection of an additional chromogenic reaction.
摘要翻译：公开了用于检测产生葡萄糖氧化酶的微生物的方法和试剂盒。该方法包括提供培养基和过氧化氢指示试剂，其包含可产生可产生葡萄糖氧化酶的微生物存在的可检测的生色反应的显色底物，并且公开了用于通过检测另外的显色性来分化微生物的另外的方法反应。

7. 发明授权

US09435739B2 Methods and compositions to detect a biological activity 有权
标题翻译：检测生物活性的方法和组合物
公开(公告)号：US09435739B2
公开(公告)日：2016-09-06
申请号：US13883620
申请日：2011-10-28
申请人： Stephen B. Roscoe , Kurt J. Halverson , Jesse D. Miller , Stephanie J. Moeller , Jason W. Bjork
发明人： Stephen B. Roscoe , Kurt J. Halverson , Jesse D. Miller , Stephanie J. Moeller , Jason W. Bjork
IPC分类号： C12Q1/44 , G01N21/64 , C12Q1/04
CPC分类号： G01N21/6486 , C12Q1/04
摘要： Compositions that comprise water, a first indicator reagent that can be converted by a first biological activity to a first biological derivative, and a plurality of particles are provided. The first indicator reagent can comprise a fluorogenic enzyme substrate having a fluorophore selected from the group consisting of umbelliferone, 7-aminocoumarin, β-naphthylamine, β-naphthol, fluorescein, resorufin, 9H-(1,3-dichloro-9,9-dimethyl acridin-2-one), rhodamine 110, a derivative of any of the foregoing fluorophores, and a combination of any of the foregoing fluorophores. The particles are capable of receiving and retaining the first biological derivative from an aqueous liquid. The first biological derivative can be indicative of a microorganism. The compositions further can comprise a gelling agent. Methods of using the compositions to detect the presence or absence of a microorganism in a sample are also provided.
摘要翻译：提供了包含水，可以通过第一生物活性转化为第一生物衍生物的第一指示剂试剂和多个颗粒的组合物。第一指示剂可以包含具有选自伞形酮，7-氨基香豆素，β-萘胺，β-萘酚，荧光素，再利用者，9H-（1,3-二氯-9,9-四氢 - 二甲基吖啶-2-酮），罗丹明110，任何前述荧光团的衍生物，以及任何上述荧光团的组合。颗粒能够从水性液体中接收和保留第一生物衍生物。第一种生物衍生物可以指示微生物。组合物还可以包含胶凝剂。还提供了使用组合物来检测样品中微生物的存在或不存在的方法。

8. 发明授权

US08518664B2 Methods of determining the amount of microorganisms present in a test sample 有权
标题翻译：确定测试样品中存在的微生物量的方法
公开(公告)号：US08518664B2
公开(公告)日：2013-08-27
申请号：US13513794
申请日：2010-12-17
申请人： Stephen B. Roscoe , Phillip A. Bolea , Stephanie J. Moeller
发明人： Stephen B. Roscoe , Phillip A. Bolea , Stephanie J. Moeller
IPC分类号： C12Q1/04
CPC分类号： G01N21/643 , C12Q1/04 , C12Q1/06
摘要： Methods of determining the amount of microorganisms present in a test sample. The methods include a) incubating the test sample with a growth media to form an incubated sample, wherein the growth media includes an enzyme substrate and the enzyme substrate includes an enzymatically hydrolyzable group and a fluorescent group, wherein microorganisms present in the test sample include an enzyme that hydrolyzes the hydrolyzable group from the fluorescent group to form a fluorescently detectable product, wherein the fluorescently detectable product has both an acidic and basic species; b) exciting the fluorescently detectable product with light having a wavelength of Exλiso for a time sufficient for the fluorescently detectable product to emit light, wherein Exλiso is the absorbance isosbestic point of the fluorescently detectable product; c) detecting light emitted at a wavelength of Emλ1; and d) quantifying the light emitted at the wavelength of Emλ1, wherein the quantity of the light emitted at the wavelength Emλ1 is indicative of the amount of microorganisms present in the test sample.
摘要翻译：确定测试样品中存在的微生物量的方法。所述方法包括：a）用生长培养基孵育所述测试样品以形成孵育的样品，其中所述生长培养基包括酶底物，并且所述酶底物包括酶促水解基团和荧光基团，其中存在于所述测试样品中的微生物包括从荧光基团水解可水解基团以形成荧光检测产物的酶，其中荧光检测产物具有酸性和碱性物质; b）用具有Exlambdaiso波长的光激发荧光检测产物足够的荧光检测产物发光的时间，其中Exlambdaiso是荧光检测产物的吸光度等值点; c）检测在波长λ1处发射的光; 以及d）量化在λ1波长处发射的光，其中在波长λ1处发射的光的量指示测试样品中存在的微生物的量。

9. 发明申请

US20120252049A1 METHODS OF DETECTING MICROORGANISMS AND KITS THEREFORE 有权
标题翻译：检测微生物和试剂盒的方法
公开(公告)号：US20120252049A1
公开(公告)日：2012-10-04
申请号：US13513794
申请日：2010-12-17
申请人： Stephen B. Roscoe , Phillip A. Bolea , Stephanie J. Moeller
发明人： Stephen B. Roscoe , Phillip A. Bolea , Stephanie J. Moeller
IPC分类号： G01N21/64 , C12M1/34
CPC分类号： G01N21/643 , C12Q1/04 , C12Q1/06
摘要： A method of detecting microorganisms in a test sample is provided. The method includes the steps of: a) incubating the test sample with a growth media to form an incubated sample, wherein the growth media includes an enzyme substrate and the enzyme substrate includes an enzymatically hydrolysable group and a fluorescent group, wherein microorganisms present in the test sample include an enzyme that hydrolyzes the hydrolysable group from the fluorescent group to form a fluorescently detectable product, wherein the fluorescently detectable product has both an acidic and basic species; b) exciting the fluorescently detectable product with light having a wavelength of Exλiso for a time sufficient for the fluorescently detectable product to emit light, wherein Exλiso is the absorbance isosbestic point of the fluorescently detectable product; and c) detecting light emitted at a wavelength of Emλ1.
摘要翻译：提供了一种检测试样中微生物的方法。该方法包括以下步骤：a）用生长培养基孵育测试样品以形成孵育的样品，其中生长培养基包括酶底物，酶底物包括酶促水解基团和荧光基团，其中存在于测试样品包括从荧光基团水解可水解基团以形成荧光可检测产物的酶，其中荧光检测产物具有酸性和碱性物质; b）用具有Exλiso波长的光激发荧光检测产物足够的荧光检测产物发光的时间，其中Exλiso是荧光检测产物的吸光度等值点; 以及c）检测在Emλ1的波长处发射的光。

10. 发明申请

US20100317020A1 METHODS AND COMPOSITIONS FOR DETECTING MICROORGANISMS 审中-公开
标题翻译：检测微生物的方法和组合物
公开(公告)号：US20100317020A1
公开(公告)日：2010-12-16
申请号：US12867091
申请日：2009-02-12
申请人： Stephen B. Roscoe , Stephanie J. Moeller , Manjiri T. Kshirsagar
发明人： Stephen B. Roscoe , Stephanie J. Moeller , Manjiri T. Kshirsagar
IPC分类号： C12Q1/68 , G01N33/569 , G01N33/53
CPC分类号： G01N33/569 , G01N33/5308 , G01N2333/39 , G01N2400/24 , G01N2469/10
摘要： Methods of analyzing a sample for target microorganisms of interest are described. In particular, the methods are useful for detecting a variety of yeast and mold microorganisms based on the presence of a ubiquitous cell wall component, such as zymosan. Methods of analyzing a sample for fungal microorganisms using liposomes and/or culture media are also described.
摘要翻译：描述了目标微生物样品分析方法。特别地，该方法可用于基于普遍存在的细胞壁组分（例如酵母聚糖）的存在来检测各种酵母和霉菌微生物。还描述了使用脂质体和/或培养基分析真菌微生物样品的方法。

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