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2. 发明授权

US5866777A Method for obtaining plants with reduced susceptibility to plant-parasitic nematodes 失效
标题翻译：获得对植物寄生线虫易感性降低的植物的方法
公开(公告)号：US5866777A
公开(公告)日：1999-02-02
申请号：US244122
申请日：1994-05-19
申请人： Peter Christiaan Sijmons , Oscar Johannes Maria Goddijn , Petrus Josephus Maria Van Den Elzen , Frederique Marianne Van Der Lee
发明人： Peter Christiaan Sijmons , Oscar Johannes Maria Goddijn , Petrus Josephus Maria Van Den Elzen , Frederique Marianne Van Der Lee
IPC分类号： C12N15/09 , A01H1/06 , A01H5/00 , A01N63/00 , C07K14/415 , C12N1/21 , C12N5/10 , C12N9/10 , C12N9/14 , C12N15/11 , C12N15/82 , C12R1/01 , C12N15/29 , A01H4/00
CPC分类号： C12N9/1029 , C07K14/415 , C12N15/8285 , C12N9/14
摘要： A method for obtaining plants with reduced susceptibility to invasion by a plant parasitic nematode, and plants having reduced susceptibility to invasion by a plant parasitic nematode obtained, for example, with such method. The method includes the following steps: (a) transforming cells of a plant or progeny of the plant with a coding sequence A and a coding sequence B, the coding sequences A and B being selected such that transformed cells expressing the coding sequences A and B display a normal phenotype and such that a nematode feeding structure comprising a cell expressing the coding sequence A but not expressing the coding sequence B has a reduced ability to support development of the plant parasitic nematode as compared with a nematode feeding structure wherein coding sequence A is not expressed, the coding sequence A being under the control of a promoter A that drives expression of the coding sequence A preferentially in the cell of the nematode feeding structure, the coding sequence B being under the control of a promoter B that drives expression of the coding sequence B in transformed cells wherein coding sequence A is expressed but not in the cell of the nematode feeding structure; (b) generating plants from the transformed cells of step (a); and (c) screening the generated plants for a transformed plant which has a reduced susceptibility to the plant parasitic nematode and which displays the normal phenotype.
摘要翻译： PCT No.PCT / EP92 / 02559 Sec。 371日期1994年5月19日 102（e）日期1994年5月19日PCT提交1992年11月2日PCT公布。公开号WO93 / 10251 日期1993年5月27日一种获得植物寄生线虫入侵敏感性降低的植物的方法，以及例如通过这种方法获得的植物寄生线虫侵袭敏感性降低的植物。该方法包括以下步骤：（a）用编码序列A和编码序列B转化植物或植物后代的细胞，选择编码序列A和B，使得表达编码序列A和B的转化细胞显示正常表型，并且使得包含表达编码序列A但不表达编码序列B的细胞的线虫饲养结构与线虫喂养结构相比具有降低的支持植物寄生线虫发育的能力，其中编码序列A为未表达的编码序列A处于驱动编码序列A的表达的启动子A的控制下，编码序列A优先在线虫饲养结构的细胞中，编码序列B在促进B的表达的启动子B的控制下在编码序列A表达但不在线虫饲养结构的细胞中的转化细胞中的编码序列B; （b）从步骤（a）的转化细胞产生植物; 和（c）筛选所产生的植物对于对植物寄生线虫具有降低的敏感性并且显示正常表型的转化植物。

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