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1. 发明申请

US20120050734A1 INVERSE-FLUORESCENCE CORRELATION SPECTROSCOPY 审中-公开
标题翻译：反相荧光相关光谱
公开(公告)号：US20120050734A1
公开(公告)日：2012-03-01
申请号：US13264651
申请日：2010-04-15
申请人： Stefan Wennmalm , Jerker Widengren
发明人： Stefan Wennmalm , Jerker Widengren
IPC分类号： G01J3/44 , G01N21/64 , G01J3/30
CPC分类号： G01N21/6408 , G01N15/0205 , G01N21/6458 , G01N21/65 , G01N21/658 , G01N2015/0038
摘要： A method is disclosed for analyzing particles or biomolecules in a liquid sample, including: detecting a signal and fluctuations in the signal from a detection volume in the sample; wherein the signal is generated from signal-generating molecules in the medium surrounding the particles or biomolecules and the fluctuations are transient reductions in the signal as the particles or biomolecules transit through the detection volume; and analyzing the detected fluctuations to obtain information about the particles or biomolecules in the liquid sample. At least one example embodiment of the present invention relates to a fluorescence correlation spectroscopy system including a laser, a zero-mode waveguide, guiding device for guiding the laser into the zero-mode waveguide, device for collecting fluorescence emission from excited molecules within the waveguide, a detector for detecting the fluorescence emission and means for autocorrelating the detected fluorescence signal, wherein the detector comprises a photomultiplier tube. Moreover, at least one embodiment relates to the use of a fluorescence correlation spectroscopy system for analyzing molecules of interest in a sample by detecting and analyzing fluctuations in a fluorescence signal that is generated from sample molecules surrounding the molecules of interest, wherein the fluctuations are transient reductions in the detected fluorescence signal.
摘要翻译：公开了一种用于分析液体样品中的颗粒或生物分子的方法，包括：检测来自样品中检测体积的信号和信号波动; 其中所述信号由围绕所述颗粒或生物分子的介质中的信号产生分子产生，并且所述波动是当所述颗粒或生物分子通过所述检测体积传递时所述信号的瞬时减少; 并分析检测到的波动以获得关于液体样品中的颗粒或生物分子的信息。本发明的至少一个示例实施例涉及一种荧光相关光谱系统，其包括激光器，零模式波导，用于将激光器引导到零模式波导中的引导装置，用于从波导内的激发分子收集荧光发射的装置，用于检测荧光发射的检测器和用于自动相关所检测的荧光信号的装置，其中所述检测器包括光电倍增管。此外，至少一个实施方案涉及通过检测和分析由感兴趣分子周围的样品分子产生的荧光信号的波动来分析样品中感兴趣的分子的荧光相关光谱系统的用途，其中波动是瞬时的检测到的荧光信号减少。

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