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1. 发明申请

US20100187438A1 Fluorescent Light Microscope For Measuring a Sample Using Red-Shifted Stokes Lines 有权
标题翻译：荧光灯显微镜用于测量样品使用红移斯托克斯线
公开(公告)号：US20100187438A1
公开(公告)日：2010-07-29
申请号：US12753349
申请日：2010-04-02
申请人： Stefan W. Hell , Brian RANKIN , Robert KELLNER , Jaydev JETHWA , Thorsten STAUDT
发明人： Stefan W. Hell , Brian RANKIN , Robert KELLNER , Jaydev JETHWA , Thorsten STAUDT
IPC分类号： G01N21/64 , G01J1/58
CPC分类号： G02B21/16 , G01N21/6458 , G01N21/65 , G01N2021/655 , H01S3/302
摘要： A fluorescent light microscope for measuring a sample comprises a light source providing transfer light having a transfer wavelength for transferring a fluorescent dye in the sample from one state into another state, and a detector which measures fluorescent light from the sample with spatial resolution. The light source comprises a laser, an optical wave guide connected to the laser, and a wavelength-selective device connected to the optical wave guide. The laser emits pump light of a pump wavelength other than the transfer wavelength and injects the pump light into the optical wave guide. The pump light, due to Raman scattering being stimulated in the optical wave guide, generates a light spectrum emerging from the optical wave guide which has, besides the pump wavelength, at least one red-shifted Stokes line whose full width at half maximum is smaller than half of its distance to its next neighbor line on the blue side of the spectrum; and the wavelength-selective device singles out the transfer light by its transfer wavelength from the red-shifted Stokes lines of the light spectrum.
摘要翻译：用于测量样品的荧光显微镜包括提供具有用于将样品中的荧光染料从一种状态转移到另一状态的转印波长的转移光，以及以空间分辨率测量来自样品的荧光的检测器。光源包括激光器，连接到激光器的光波导和连接到光波导的波长选择器件。激光器发射除了转移波长之外的泵波长的泵浦光，并将泵浦光注入到光波导中。由于在光波导中被拉曼散射的泵浦光产生从光波导出射的光谱，除了泵浦波长之外，还有至少一个红移斯托克斯线，其半峰全宽较小超过其在光谱蓝色边上的下一个相邻线的距离的一半; 并且波长选择装置通过其来自光谱的红移斯托克斯线的转移波长来分离转移光。

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