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1. 发明授权

US5948328A Shaping of microparticles in electric-field cages 失效
标题翻译：电场笼中微粒的形成
公开(公告)号：US5948328A
公开(公告)日：1999-09-07
申请号：US700395
申请日：1996-08-23
申请人： Stefan Fiedler , Andreas Voigt , Thomas Schnelle , Guenter Fuhr , Torsten Mueller , Rolf Hagedorn , Jan Hornung , Henning Glasser , Bernd Wagner
发明人： Stefan Fiedler , Andreas Voigt , Thomas Schnelle , Guenter Fuhr , Torsten Mueller , Rolf Hagedorn , Jan Hornung , Henning Glasser , Bernd Wagner
IPC分类号： B01J2/00 , B01J13/02 , B01J19/08
CPC分类号： B01J13/02 , B01J19/087 , B01J2/00
摘要： In the method proposed, microparticles suspended in a liquid or droplets suspended in a liquid with which they are immiscible are shaped by high-frequency electric fields in a three-dimensional electrode array of a size in the micrometer or submicrometer range and subsequently consolidated by prior art chemical bonding procedures or by physical methods. The disposition, geometry and control of the electrodes determine the shape of the particles. The particles themselves must have a conductivity and/or relative dielectric constant lower than the solution surrounding them. For some, this can be achieved only at certain frequencies in the kHz and MHz band which are determined by the passive electrical properties of the particles and the surrounding solution. The particles or droplets are repelled by the electrodes so that they are shaped in the free solution without making contact with any surface and can then be consolidated. This makes it possible to shape micrometer and submicrometer size particles of the kind required in chromatography, affinity biochemistry and medicine, as well as for filter systems.
摘要翻译： PCT No.PCT / DE95 / 00237 Sec。 371日期：1996年8月23日 102（e）日期1996年8月23日PCT提交1995年2月23日PCT公布。 WO95 / 23020 PCT公开号日期1995年8月31日在所提出的方法中，悬浮在液体中的微粒悬浮在与其不混溶的液体中的微滴通过在微米或亚微米范围内的尺寸的三维电极阵列中的高频电场并随后通过现有技术的化学键合方法或通过物理方法进行固结。电极的布置，几何形状和控制决定了颗粒的形状。颗粒本身必须具有低于围绕它们的溶液的导电性和/或相对介电常数。对于一些，这只能在通过颗粒和周围溶液的被动电特性确定的kHz和MHz频带的某些频率下实现。颗粒或液滴被电极排斥，使得它们在游离溶液中成形，而不与任何表面接触，然后可以被固结。这使得可以形成色谱，亲和力生物化学和药物所需类型的微米和亚微米尺寸的颗粒，以及过滤系统。

2. 发明申请

US20050120235A1 Methods and devices for controlling the access to a data processing device 审中-公开
标题翻译：用于控制对数据处理设备的访问的方法和设备
公开(公告)号：US20050120235A1
公开(公告)日：2005-06-02
申请号：US10502164
申请日：2003-01-22
申请人： Heiko Zimmermann , Guenter Fuhr , Rolf Hagedorn
发明人： Heiko Zimmermann , Guenter Fuhr , Rolf Hagedorn
IPC分类号： G06F1/00 , G06F1/20 , G06F11/00 , G06F11/30 , G06F12/14 , G11C5/00 , H04L9/00 , H04L9/32
CPC分类号： G06F11/00 , G06F1/20
摘要： A method and devices for controlling access to a data processing device, in particular a data memory, having an access filter circuit (10) are described, whereby the access filter circuit (10) is operated with enabled access in a read-write mode at a normal operating temperature and with limited access at an access protection temperature which is lower than the normal operating temperature.
摘要翻译：描述了一种用于控制对具有访问滤波器电路（10）的数据处理设备，特别是数据存储器的访问的方法和设备，由此访问滤波器电路（10）在读写模式下以启用的访问正常工作温度，并且在低于正常工作温度的访问保护温度下具有有限的访问。

3. 发明申请

US20100196946A1 DEVICE AND METHOD FOR CONDITIONING BIOLOGICAL CELLS 有权
标题翻译：用于调节生物细胞的装置和方法
公开(公告)号：US20100196946A1
公开(公告)日：2010-08-05
申请号：US12678544
申请日：2008-09-17
申请人： Guenter Fuhr , Heiko Zimmermann
发明人： Guenter Fuhr , Heiko Zimmermann
IPC分类号： C12Q1/02 , C12M1/00
CPC分类号： C12M35/02
摘要： A conditioning device (100) for biological cells includes a support (10), adapted to support at least one biological cell (1) in a supporting zone (11), and a contact device (20), adapted to support at least one conditioning sample (3) and to displace the at least one conditioning sample (3) relative to the support (10) in such a manner that the at least one conditioning sample (3) touches the at least one biological cell (1), the contact device (20) including at least two mechanical supporting elements (21, 22) that can be displaced from various spatial directions towards the support (10). The invention also relates to methods for conditioning, especially imprinting or differentiating biological cells and to uses of the conditioning device.
摘要翻译：用于生物细胞的调节装置（100）包括适于在支撑区域（11）中支撑至少一个生物细胞（1）的支撑件（10）和适于支撑至少一个调理样品（3）并相对于载体（10）置换至少一个调理样品（3），使得至少一个调理样品（3）接触至少一个生物细胞（1），接触装置（20）包括至少两个机械支撑元件（21,22），所述至少两个机械支撑元件可以从各个空间方向朝支撑件（10）移位。本发明还涉及用于调理，特别是印记或分化生物细胞的方法以及调理装置的用途。

4. 发明授权

US08546117B2 Method and device for forming a three-dimensional arrangement of biological cells 有权
标题翻译：用于形成生物细胞三维排列的方法和装置
公开(公告)号：US08546117B2
公开(公告)日：2013-10-01
申请号：US12664933
申请日：2008-06-06
申请人： Charli Kruse , Philipp Ciba , Guenter Fuhr
发明人： Charli Kruse , Philipp Ciba , Guenter Fuhr
IPC分类号： C12N11/00 , C12N11/16 , C12P1/00
CPC分类号： C12N5/0068 , C12N5/0062 , C12N2533/30
摘要： A method for forming a three-dimensional cell arrangement (1) of biological cells is disclosed, including the steps of preparation of the cell arrangement (1) on a flexible substrate (10) and deformation of the substrate (10), wherein the deformation of the substrate (10) is brought about by an attractive force exerted by the cells (2) on the substrate (10). A substrate (10), is also disclosed, made from a flexible material and including a substrate surface (11) for adhesion of a cell arrangement (1) of biological cells, wherein the substrate surface (11) has a number of force attachment points arranged to exert an attraction force which may be transmitted from the cells to the substrate (10) and the substrate (10) has a flexibility such that the substrate (10) is deformable with the action of the attraction force.
摘要翻译：公开了一种用于形成生物细胞的三维细胞装置（1）的方法，包括在柔性基底（10）上制备细胞装置（1）和基底（10）变形的步骤，其中变形通过由电池（2）施加在衬底（10）上的吸引力引起衬底（10）的移动。还公开了由柔性材料制成并且包括用于粘附生物细胞的细胞装置（1）的基底表面（11）的基底（10），其中所述基底表面（11）具有多个力附着点布置成施加可以从单元传递到基板（10）的吸引力，并且基板（10）具有柔性，使得基板（10）可以在吸引力的作用下变形。

5. 发明授权

US07892815B2 Device and method for conditioning biological cells 有权
标题翻译：生物细胞调理装置及方法
公开(公告)号：US07892815B2
公开(公告)日：2011-02-22
申请号：US12678544
申请日：2008-09-17
申请人： Guenter Fuhr , Heiko Zimmermann
发明人： Guenter Fuhr , Heiko Zimmermann
IPC分类号： C12M1/34 , C12M3/00
CPC分类号： C12M35/02
摘要： A conditioning device (100) for biological cells includes a support (10), adapted to support at least one biological cell (1) in a supporting zone (11), and a contact device (20), adapted to support at least one conditioning sample (3) and to displace the at least one conditioning sample (3) relative to the support (10) in such a manner that the at least one conditioning sample (3) touches the at least one biological cell (1), the contact device (20) including at least two mechanical supporting elements (21, 22) that can be displaced from various spatial directions towards the support (10). The invention also relates to methods for conditioning, especially imprinting or differentiating biological cells and to uses of the conditioning device.
摘要翻译：用于生物细胞的调节装置（100）包括适于在支撑区域（11）中支撑至少一个生物细胞（1）的支撑件（10）和适于支撑至少一个调理样品（3）并相对于载体（10）置换至少一个调理样品（3），使得至少一个调理样品（3）接触至少一个生物细胞（1），接触装置（20）包括至少两个机械支撑元件（21,22），所述至少两个机械支撑元件可以从各个空间方向朝支撑件（10）移位。本发明还涉及用于调理，特别是印记或分化生物细胞的方法以及调理装置的用途。

6. 发明申请

US20100190249A1 METHOD AND DEVICE FOR FORMING A THREE-DIMENSIONAL ARRANGEMENT OF BIOLOGICAL CELLS 有权
标题翻译：用于形成生物细胞三维布置的方法和装置
公开(公告)号：US20100190249A1
公开(公告)日：2010-07-29
申请号：US12664933
申请日：2008-06-06
申请人： Charli Kruse , Philipp Ciba , Guenter Fuhr
发明人： Charli Kruse , Philipp Ciba , Guenter Fuhr
IPC分类号： C12N5/071
CPC分类号： C12N5/0068 , C12N5/0062 , C12N2533/30
摘要： A method for forming a three-dimensional cell arrangement (1) of biological cells is disclosed, including the steps of preparation of the cell arrangement (1) on a flexible substrate (10) and deformation of the substrate (10), wherein the deformation of the substrate (10) is brought about by an attractive force exerted by the cells (2) on the substrate (10). A substrate (10), is also disclosed, made from a flexible material and including a substrate surface (11) for adhesion of a cell arrangement (1) of biological cells, wherein the substrate surface (11) has a number of force attachment points arranged to exert an attraction force which may be transmitted from the cells to the substrate (10) and the substrate (10) has a flexibility such that the substrate (10) is deformable with the action of the attraction force.
摘要翻译：公开了一种用于形成生物细胞的三维细胞装置（1）的方法，包括在柔性基底（10）上制备细胞装置（1）和基底（10）变形的步骤，其中变形通过由电池（2）施加在衬底（10）上的吸引力引起衬底（10）的移动。还公开了由柔性材料制成并且包括用于粘附生物细胞的细胞装置（1）的基底表面（11）的基底（10），其中所述基底表面（11）具有多个力附着点布置成施加可以从单元传递到基板（10）的吸引力，并且基板（10）具有柔性，使得基板（10）可以在吸引力的作用下变形。

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