专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20120177603A1 METHOD FOR THE PURIFICATION OF INTERFERON-B 审中-公开
标题翻译：干扰素B的纯化方法
公开(公告)号：US20120177603A1
公开(公告)日：2012-07-12
申请号：US13382807
申请日：2010-07-07
申请人： Stefan Arnold , Christian Scheckermann
发明人： Stefan Arnold , Christian Scheckermann
IPC分类号： A61K38/21 , C07K14/565
CPC分类号： C07K14/565 , A61K9/0019 , A61K38/00
摘要： A method for the production of human glycosylated Interferon-beta (IFN-β) is described, comprising two affinity chromatography steps followed by hydrophobic interaction chromatography step preferably with a subsequent anion exchange chromatography step. IFN-β obtained by the method as described is characterized by a high purity and specific biologically activity, because of which it is particularly suitable for the production of pharmaceutical compositions.
摘要翻译：描述了用于生产人糖基化干扰素-β（IFN-α）的方法，其包括两个亲和层析步骤，随后是疏水相互作用层析步骤，优选随后进行阴离子交换层析步骤。 IFN-和bgr 通过所述方法获得的特征在于高纯度和特异性的生物活性，因为它特别适用于制备药物组合物。

2. 发明申请

US20120135928A1 METHOD FOR PURIFYING RECOMBINANT FSH 有权
标题翻译：净化重组FSH的方法
公开(公告)号：US20120135928A1
公开(公告)日：2012-05-31
申请号：US13262622
申请日：2010-04-01
申请人： Christian Scheckermann , Dietmar Eichinger , Stefan Arnold
发明人： Christian Scheckermann , Dietmar Eichinger , Stefan Arnold
IPC分类号： A61K38/24 , A61P15/08 , C07K14/59 , C07K1/36 , B01D15/38
CPC分类号： C07K14/59 , A61K38/00 , C07K1/36 , C12P21/005
摘要： The present invention relates to a method for purifying a recombinant follicle stimulating hormone (FSH) or re-combinant FSH variant. The method comprises the steps of subjecting a liquid containing a recombinant FSH or recombinant FSH variant to an anion exchange chromatography, to a hydrophobic interaction chromatography, and to a dye affinity chromatography, wherein these chromatographies may be performed in any order, and wherein the method neither comprises a weak anion exchange chromatography nor a reverse phase chromatography. The method of purification results in a high yield of recombinant FSH having a desired degree of purity. The obtained FSH is especially useful for the prophylaxis and treatment of disorders and medical indications where FSH preparations are considered as useful remedies.
摘要翻译：本发明涉及一种纯化重组卵泡刺激素（FSH）或重组FSH变体的方法。该方法包括以下步骤：将含有重组FSH或重组FSH变体的液体进行阴离子交换层析，到疏水相互作用色谱法和染料亲和层析，其中这些色谱可以以任何顺序进行，并且其中所述方法既不包括弱阴离子交换色谱法也不包括反相色谱法。纯化的方法导致具有所需纯度的重组FSH的高产率。获得的FSH对于预防和治疗疾病和医疗适应症尤其有用，其中FSH制剂被认为是有用的补救措施。

3. 发明授权

US09096683B2 Method for purifying recombinant FSH 有权
标题翻译：重组FSH的纯化方法
公开(公告)号：US09096683B2
公开(公告)日：2015-08-04
申请号：US13262622
申请日：2010-04-01
申请人： Christian Scheckermann , Dietmar Eichinger , Stefan Arnold
发明人： Christian Scheckermann , Dietmar Eichinger , Stefan Arnold
IPC分类号： A61K38/24 , A61P15/08 , C07K1/36 , C07K14/59
CPC分类号： C07K14/59 , A61K38/00 , C07K1/36 , C12P21/005
摘要： The present invention relates to a method for purifying a recombinant follicle stimulating hormone (FSH) or recombinant FSH variant. The method comprises the steps of subjecting a liquid containing a recombinant FSH or recombinant FSH variant to an anion exchange chromatography, to a hydrophobic interaction chromatography, and to a dye affinity chromatography, wherein these chromatographies may be performed in any order, and wherein the method neither comprises a weak anion exchange chromatography nor a reverse phase chromatography. The method of purification results in a high yield of recombinant FSH having a desired degree of purity. The obtained FSH is especially useful for the prophylaxis and treatment of disorders and medical indications where FSH preparations are considered as useful remedies.
摘要翻译：本发明涉及纯化重组卵泡刺激素（FSH）或重组FSH变体的方法。该方法包括以下步骤：将含有重组FSH或重组FSH变体的液体进行阴离子交换层析，到疏水相互作用层析和染料亲和层析，其中这些色谱可以以任何顺序进行，并且其中所述方法既不包括弱阴离子交换色谱法也不包括反相色谱法。纯化的方法导致具有所需纯度的重组FSH的高产率。获得的FSH对于预防和治疗疾病和医疗适应症尤其有用，其中FSH制剂被认为是有用的补救措施。

4. 发明授权

US08318457B2 FSH producing cell clone 有权
标题翻译： FSH产生细胞克隆
公开(公告)号：US08318457B2
公开(公告)日：2012-11-27
申请号：US12666257
申请日：2008-06-27
申请人： Stefan Arnold , Nanni Jelinek
发明人： Stefan Arnold , Nanni Jelinek
IPC分类号： A61K38/24 , C07K1/00 , C12N5/00 , C12N5/071 , C12N15/09 , C12N15/16 , C12P21/00
CPC分类号： C07K14/59 , C12N15/85
摘要： The present invention relates to nucleic acid molecules comprising a nucleic acid sequence coding for the α- and the β-chain of the human follicle stimulating hormone (FSH), respectively, which has been modified with respect to the codon usage in CHO cells. The present invention further relates to a recombinant nucleic acid molecule comprising such nucleic acid sequences and host cells containing such recombinant nucleic acid molecules, as well as their use in the production of recombinant human FSH. Finally, the present invention also relates to a method for producing host cells expressing human follicle stimulating hormone by transfecting cells in suspension culture under serum-free conditions with the recombinant nucleic acid molecule of the present invention.
摘要翻译：本发明涉及分别包含编码人卵泡刺激素（FSH）的α-和β-链的核酸序列的核酸分子，其已针对CHO细胞中的密码子使用进行了修饰。本发明还涉及包含这种核酸序列的重组核酸分子和含有这种重组核酸分子的宿主细胞，以及它们在重组人FSH的生产中的用途。最后，本发明还涉及通过用本发明的重组核酸分子在无血清条件下在悬浮培养中转染细胞来产生表达人卵泡刺激素的宿主细胞的方法。

5. 发明授权

US07468351B2 Erythropoietin solution formulation 有权
标题翻译：促红细胞生成素溶液制剂
公开(公告)号：US07468351B2
公开(公告)日：2008-12-23
申请号：US10581269
申请日：2004-12-01
申请人： Stefan Arnold , Okke Franssen , Albert Mekking
发明人： Stefan Arnold , Okke Franssen , Albert Mekking
IPC分类号： A61K38/00 , A61K38/18 , C07K14/505
CPC分类号： A61K38/1816 , A61K9/0019 , A61K47/02 , A61K47/18
摘要： A stable pharmaceutical formulation of erythropoietin is disclosed which contains tris-(hydrox-ymethyl)-aminomethane as stabilizer, whereby the formulation does not contain amino acids or human serumalbumin.
摘要翻译：公开了稳定的促红细胞生成素药物制剂，其含有三 - （羟基 - 甲基） - 氨基甲烷作为稳定剂，由此该制剂不含氨基酸或人血清白蛋白。

6. 发明申请

US20120264688A1 PROCESS FOR THE PURIFICATION OF RECOMBINANT HUMAN ERYTHROPOIETIN (EPO), EPO THUS PURIFIED AND PHARMACEUTICAL COMPOSITIONS COMPRISING SAME 审中-公开
标题翻译：重组人ERYTHROPOIETIN（EPO），EPO THUS纯化和包含其的药物组合物的纯化方法
公开(公告)号：US20120264688A1
公开(公告)日：2012-10-18
申请号：US13497500
申请日：2010-09-23
申请人： Walter Hinderer , Stefan Arnold
发明人： Walter Hinderer , Stefan Arnold
IPC分类号： C07K1/36 , A61K38/18 , C07K14/505
CPC分类号： C07K14/505 , A61K38/00
摘要： A procedure for the production of erythropoietin (EPO), in particular recombinant human EPO (rhEPO) with a defined composition of glycoforms in a highly pure form, i.e., with a high amount of O-glycosylated EPO isoforms is provided.
摘要翻译：提供了以高纯度形式即具有大量O-糖基化EPO同种型的糖形式制备促红细胞生成素（EPO），特别是重组人EPO（rhEPO）的方法。

7. 发明申请

US20120034655A1 FSH PRODUCING CELL CLONE 有权
标题翻译： FSH生产细胞克隆
公开(公告)号：US20120034655A1
公开(公告)日：2012-02-09
申请号：US12666257
申请日：2008-06-27
申请人： Stefan Arnold , Nanni Jelinek
发明人： Stefan Arnold , Nanni Jelinek
IPC分类号： C12P21/00 , C12N5/10 , C12N15/09 , C12N15/16
CPC分类号： C07K14/59 , C12N15/85
摘要： The present invention relates to nucleic acid molecules comprising a nucleic acid sequence coding for the α- and the β-chain of the human follicle stimulating hormone (FSH), respectively, which has been modified with respect to the codon usage in CHO cells. The present invention further relates to a recombinant nucleic acid molecule comprising such nucleic acid sequences and host cells containing such recombinant nucleic acid molecules, as well as their use in the production of recombinant human FSH. Finally, the present invention also relates to a method for producing host cells expressing human follicle stimulating hormone by transfecting cells in suspension culture under serum-free conditions with the recombinant nucleic acid molecule of the present invention.
摘要翻译：本发明涉及分别包含编码人卵泡刺激素（FSH）的α-和β-链的核酸序列的核酸分子，其已针对CHO细胞中的密码子使用进行了修饰。本发明还涉及包含这种核酸序列的重组核酸分子和含有这种重组核酸分子的宿主细胞，以及它们在重组人FSH的生产中的用途。最后，本发明还涉及通过用本发明的重组核酸分子在无血清条件下在悬浮培养中转染细胞来产生表达人卵泡刺激素的宿主细胞的方法。

8. 发明申请

US20070128231A1 Erythropoietin solution formulation 有权
标题翻译：促红细胞生成素溶液制剂
公开(公告)号：US20070128231A1
公开(公告)日：2007-06-07
申请号：US10581269
申请日：2004-12-01
申请人： Stefan Arnold , Okke Franssen , Albert Mekking
发明人： Stefan Arnold , Okke Franssen , Albert Mekking
IPC分类号： A61K38/18 , A61K9/00
CPC分类号： A61K38/1816 , A61K9/0019 , A61K47/02 , A61K47/18
摘要： A stable pharmaceutical formulation of erythropoietin is disclosed which contains tris-(hydnox-ymethyl)-aminomethane as stabilizer, whereby the formulation does not contain amino acids or human serumalbumin.
摘要翻译：公开了一种稳定的促红细胞生成素药物制剂，其含有三（羟基 - 甲基） - 氨基甲烷作为稳定剂，由此该制剂不含氨基酸或人血清白蛋白。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式