专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06537548B1 Antibodies specific for ungulate PrP 失效
标题翻译：针对有蹄类PrP的特异性抗体
公开(公告)号：US06537548B1
公开(公告)日：2003-03-25
申请号：US09627218
申请日：2000-07-27
申请人： Stanley B. Prusiner , Jiri Safar , R. Anthony Williamson , Dennis R. Burton
发明人： Stanley B. Prusiner , Jiri Safar , R. Anthony Williamson , Dennis R. Burton
IPC分类号： A61K39395
CPC分类号： C07K16/2872 , C07K16/18 , C07K2317/34 , C07K2317/55 , G01N33/6896 , G01N2800/2828
摘要： The present invention provides antibodies that specifically bind with a high degree of binding affinity to a native ungulate PrPC and/or a denatured ungulate PrPSc, but not to a native ungulate PrPSc. Preferred antibodies find native bovine PrPC and treated PrPSc but not native bovine PrPSc and can be used in an assay to determine if a sample is infected with infectious prions, i.e. PrPSc.
摘要翻译：本发明提供了与天然有蹄类动物PrPC和/或变性的有蹄类动物PrPSc具有高度结合亲和力的特异性结合的抗体，而不是天然的有蹄类PrPSc结合的抗体。优选的抗体发现天然牛PrPC和处理的PrPSc而不是天然牛PrPSc，并且可用于测定以确定样品是否感染有感染性朊病毒，即PrPSc。

2. 发明授权

US07052675B2 Antibodies specific for native PrPSc 失效
标题翻译：针对天然PrPSc特异的抗体
公开(公告)号：US07052675B2
公开(公告)日：2006-05-30
申请号：US11027139
申请日：2004-12-29
申请人： Stanley B. Prusiner , R. Anthony Williamson , Dennis R. Burton
发明人： Stanley B. Prusiner , R. Anthony Williamson , Dennis R. Burton
IPC分类号： A61K39/395 , G01N33/53 , G01N33/567 , C07K16/00
CPC分类号： C07K16/18 , C07K2317/55 , G01N33/56983 , G01N33/6896 , G01N2800/2828
摘要： Antibodies are disclosed which specifically bind to native PrPSc in situ. Preferred antibodies bind only to the native PrPSc of a particular species e.g., human, cow, sheep, pig, etc. Particularly preferred antibodies bind specifically to a particular isoform of human PrPSc. Preferred antibodies of the invention are (1) produced by phage display methodology, (2) bind specifically to native PrPSc, (3) neutralizes the infectivity of prions, (4) bind to PrPSc in situ and (5) bind 50% or more of PrPSc in a liquid flowable sample. Antibodies of the invention can be bound to a substrate and used to assay a sample (which has any PrPc denatured via proteinase K) for the presence of PrPSc of a specific species which PrPSc is associated with disease. Antibodies which specifically bind to human PrPSc can be labeled and injected carrying out an in vivo diagnostic test to determine if the human is infected with prions associated with disease. The antibodies are preferably produced using phage display technology wherein the genetic material in the phage expressing the antibody is obtained from a mammal with an ablated endogenous PrP protein gene and an endogenous chimeric PrP gene which mammal had been inoculated with PrPSc to induce antibody production.
摘要翻译：公开了原位特异性结合天然PrP Sc的抗体。优选的抗体仅结合特定种类例如人，牛，绵羊，猪等的天然PrP Sc。特别优选的抗体特异性结合人PrP < SUP>。本发明优选的抗体是（1）通过噬菌体展示法产生，（2）特异性结合天然PrP ，（3）中和朊病毒感染性，（4）结合PrP < Sc（5）在液体可流动的样品中结合50％以上的PrP Sc 。本发明的抗体可以与底物结合并用于测定样品（其通过蛋白酶K变性的任何PrP c），以获得特异性的PrP PrP Sc 与疾病相关的物种。可以标记并注射与人PrP标准物质特异性结合的抗体进行体内诊断测试，以确定人是否感染与疾病相关的朊病毒。优选使用噬菌体展示技术产生抗体，其中表达抗体的噬菌体中的遗传物质是从具有消融的内源性PrP蛋白基因的哺乳动物和内源性嵌合PrP基因获得的，哺乳动物已用PrP < SUP>以诱导抗体产生。

3. 发明授权

US06858397B2 Antibodies specific for native PrPsc 失效
标题翻译：特异于天然PrPsc的抗体
公开(公告)号：US06858397B2
公开(公告)日：2005-02-22
申请号：US10435602
申请日：2003-05-09
申请人： Stanley B. Prusiner , R. Anthony Williamson , Dennis R. Burton
发明人： Stanley B. Prusiner , R. Anthony Williamson , Dennis R. Burton
IPC分类号： G01N33/53 , C07K16/18 , C07K16/44 , C12N5/10 , C12N15/02 , C12N15/09 , C12P21/08 , G01N33/569 , G01N33/68 , A61K39/395 , C07K16/00 , G01N33/567
CPC分类号： C07K16/18 , C07K2317/55 , G01N33/56983 , G01N33/6896 , G01N2800/2828
摘要： Antibodies are disclosed which specifically bind to native PrPSc in situ. Preferred antibodies bind only to the native PrPSc of a particular species e.g., human, cow, sheep, pig, etc. Particularly preferred antibodies bind specifically to a particular isoform of human PrPSc. Preferred antibodies of the invention are (1) produced by phage display methodology, (2) bind specifically to native PrPSc, (3) neutralizes the infectivity of prions, (4) bind to PrPSc in situ and (5) bind 50% or more of PrPSc in a liquid flowable sample. Antibodies of the invention can be bound to a substrate and used to assay a sample (which has any PrPc denatured via proteinase K) for the presence of PrPSc of a specific species which PrPSc is associated with disease. Antibodies which specifically bind to human PrPSc can be labeled and injected carrying out an in vivo diagnostic test to determine if the human is infected with prions associated with disease. The antibodies are preferably produced using phage display technology wherein the genetic material in the phage expressing the antibody is obtained from a mammal with an ablated endogenous PrP protein gene and an endogenous chimeric PrP gene which mammal had been inoculated with PrPSc to induce antibody production.
摘要翻译：公开了与原位PrP 特异性结合的抗体。优选的抗体仅与特定物种例如人，牛，绵羊，猪等的天然PrP 。本发明的抗体可以与底物结合并用于测定样品（其通过蛋白酶K变性的任何PrPc）存在PrP Sc与PrP Sc相关的特定物种的PrP 。可以对与人PrP 特异性结合的抗体进行标记和注射进行体内诊断测试，以确定人是否感染与疾病相关的朊病毒。优选使用噬菌体展示技术产生抗体，其中表达抗体的噬菌体中的遗传物质是从具有消融的内源性PrP蛋白基因的哺乳动物和内源性嵌合PrP基因获得的，哺乳动物用PrP 接种以诱导抗体生产。

4. 发明授权

US06562341B2 Antibodies specific for native PrPSc 失效
公开(公告)号：US06562341B2
公开(公告)日：2003-05-13
申请号：US09943906
申请日：2001-08-30
申请人： Stanley B. Prusiner , R. Anthony Williamson , Dennis R. Burton
发明人： Stanley B. Prusiner , R. Anthony Williamson , Dennis R. Burton
IPC分类号： A61K39395
CPC分类号： C07K16/18 , C07K2317/55 , G01N33/56983 , G01N33/6896 , G01N2800/2828
摘要： Antibodies are disclosed which specifically bind to native PrPSc in situ. Preferred antibodies bind only to the native PrPSc of a particular species e.g., human, cow, sheep, pig, etc. Particularly preferred antibodies bind specifically to a particular isoform of human PrPSc. Preferred antibodies of the invention are (1) produced by phage display methodology, (2) bind specifically to native PrPSc, (3) neutralizes the infectivity of prions, (4) bind to PrPSc in situ and (5) bind 50% or more of PrPSc in a liquid flowable sample. Antibodies of the invention can be bound to a substrate and used to assay a sample (which has any PrPc denatured via proteinase K) for the presence of PrPSc of a specific species which PrPSc is associated with disease. Antibodies which specifically bind to human PrPSc can be labeled and injected carrying out an in vivo diagnostic test to determine if the human is infected with prions associated with disease. The antibodies are preferably produced using phage display technology wherein the genetic material in the phage expressing the antibody is obtained from a mammal with an ablated endogenous PrP protein gene and an endogenous chimeric PrP gene which mammal had been inoculated with PrPSc to induce antibody production.

5. 发明授权

US07094553B2 Antibodies specific for ungulate PrP 失效
标题翻译：针对有蹄类PrP的特异性抗体
公开(公告)号：US07094553B2
公开(公告)日：2006-08-22
申请号：US10355780
申请日：2003-01-30
申请人： Stanley B. Prusiner , Jiri G. Safar , R. Anthony Williamson , Dennis R. Burton
发明人： Stanley B. Prusiner , Jiri G. Safar , R. Anthony Williamson , Dennis R. Burton
IPC分类号： G01N33/53
CPC分类号： C07K16/2872 , C07K16/18 , C07K2317/34 , C07K2317/55 , G01N33/6896 , G01N2800/2828
摘要： The present invention provides antibodies that specifically bind with a high degree of binding affinity to a native ungulate PrPC and/or a denatured ungulate PrPSc, but not to a native ungulate PrPSc. Preferred antibodies find native bovine PrPC and treated PrPSc but not native bovine PrPSc and can be used in an assay to determine if a sample is infected with infectious prions, i.e. PrPSc.
摘要翻译：本发明提供了与天然有蹄类动物PrP和/或变性有蹄类动物PrP 特异性结合的抗体，而不是天然有蹄类动物 PrP ^{Sc 。优选的抗体发现天然牛PrP和治疗的PrP 而不是天然牛PrP Sc Sc，并且可用于测定中以确定样品感染了朊病毒，即PrP ^{Sc 。}}

6. 发明授权

US06372214B1 Antibodies specific for native PrPSc 失效
标题翻译：针对天然PrPSc特异的抗体
公开(公告)号：US06372214B1
公开(公告)日：2002-04-16
申请号：US09550374
申请日：2000-04-13
申请人： Stanley B. Prusiner , R. Anthony Williamson , Dennis R. Burton
发明人： Stanley B. Prusiner , R. Anthony Williamson , Dennis R. Burton
IPC分类号： A61K39395
CPC分类号： C07K16/18 , C07K2317/55 , G01N33/56983 , G01N33/6896 , G01N2800/2828
摘要： Antibodies are disclosed which specifically bind to native PrPSc in situ. Preferred antibodies bind only to the native PrPSc of a particular species e.g., human, cow, sheep, pig, etc. Particularly preferred antibodies bind specifically to a particular isoform of human PrPSc. Preferred antibodies of the invention are (1) produced by phage display methodology, (2) bind specifically to native PrPSc, (3) neutralizes the infectivity of prions, (4) bind to PrPSc in situ and (5) bind 50% or more of PrPSc in a liquid flowable sample. Antibodies of the invention can be bound to a substrate and used to assay a sample (which has any PrPSc denatured via proteinase K) for the presence of PrPSc of a specific species which PrPSc is associated with disease. Antibodies which specifically bind to human PrPSc can be labeled and injected carrying out an in vivo diagnostic test to determine if the human is infected with prions associated with disease. The antibodies are preferably produced using phage display technology wherein the genetic material in the phage expressing the antibody is obtained from a mammal with an ablated endogenous PrP protein gene and an endogenous chimeric PrP gene which mammal had been inoculated with PrPSc to induce antibody production.
摘要翻译：公开了与原位PrPSc特异性结合的抗体。优选的抗体仅结合特定物种例如人，牛，绵羊，猪等的天然PrPSc。特别优选的抗体特异性结合人PrPSc的特异性同种型。本发明优选的抗体是（1）通过噬菌体展示法产生，（2）特异性结合天然PrPSc，（3）中和朊病毒感染性，（4）原位结合PrPSc，（5）结合50％以上的PrPSc在液体可流动的样品中。本发明的抗体可以与底物结合并用于测定PrPSc与疾病相关的特定物种的PrPSc的存在的样品（其通过蛋白酶K变性的任何PrPSc）。可以对与PrPSc特异性结合的抗体进行标记和注射进行体内诊断测试，以确定人是否感染了与疾病相关的朊病毒。优选使用噬菌体展示技术制备抗体，其中表达抗体的噬菌体中的遗传物质是从具有消融的内源性PrP蛋白基因的哺乳动物和内源性嵌合PrP基因获得的，哺乳动物已用PrPSc接种以诱导抗体产生。

7. 发明授权

US06290954B1 Antibodies specific for native PrPSc 失效
标题翻译：针对天然PrPSc特异的抗体
公开(公告)号：US06290954B1
公开(公告)日：2001-09-18
申请号：US09036579
申请日：1998-03-06
申请人： Stanley B. Prusiner , R. Anthony Williamson , Dennis R. Burton
发明人： Stanley B. Prusiner , R. Anthony Williamson , Dennis R. Burton
IPC分类号： A61K39395
CPC分类号： C07K16/18 , C07K2317/55 , G01N33/56983 , G01N33/6896 , G01N2800/2828
摘要： Antibodies are disclosed which specifically bind to native PrPSc in situ. Preferred antibodies bind only to the native PrPSc of a particular species e.g., human, cow, sheep, pig, etc. Particularly preferred antibodies bind specifically to a particular isoform of human PrPSc. Preferred antibodies of the invention are (1) produced by phage display methodology, (2) bind specifically to native PrPSc, (3) neutralizes the infectivity of prions, (4) bind to PrPSc in situ and (5) bind 50% or more of PrPSc in a liquid flowable sample. Antibodies of the invention can be bound to a substrate and used to assay a sample (which has any PrPc denatured via proteinase K) for the presence of PrPSc of a specific species which PrPSc is associated with disease. Antibodies which specifically bind to human PrPSc can be labeled and injected carrying out an in vivo diagnostic test to determine if the human is infected with prions associated with disease. The antibodies are preferably produced using phage display technology wherein the genetic material in the phage expressing the antibody is obtained from a mammal with an ablated endogenous PrP protein gene and an endogenous chimeric PrP gene which mammal had been inoculated with PrPSc to induce antibody production.
摘要翻译：公开了与原位PrPSc特异性结合的抗体。优选的抗体仅结合特定物种例如人，牛，绵羊，猪等的天然PrPSc。特别优选的抗体特异性结合人PrPSc的特异性同种型。本发明优选的抗体是（1）通过噬菌体展示法产生，（2）特异性结合天然PrPSc，（3）中和朊病毒感染性，（4）原位结合PrPSc，（5）结合50％以上的PrPSc在液体可流动的样品中。本发明的抗体可以结合于底物并用于测定PrPSc与PrPSc与疾病相关的特定物种的PrPSc的存在的样品（其具有通过蛋白酶K变性的任何PrPc）。可以对与PrPSc特异性结合的抗体进行标记和注射进行体内诊断测试，以确定人是否感染了与疾病相关的朊病毒。优选使用噬菌体展示技术制备抗体，其中表达抗体的噬菌体中的遗传物质是从具有消融的内源性PrP蛋白基因的哺乳动物和内源性嵌合PrP基因获得的，哺乳动物已用PrPSc接种以诱导抗体产生。

8. 发明授权

US5846533A Antibodies specific for native PrP.sup.Sc 失效
公开(公告)号：US5846533A
公开(公告)日：1998-12-08
申请号：US713939
申请日：1996-09-13
申请人： Stanley B. Prusiner , R. Anthony Williamson , Dennis R. Burton
发明人： Stanley B. Prusiner , R. Anthony Williamson , Dennis R. Burton
IPC分类号： G01N33/53 , C07K16/18 , C07K16/44 , C12N5/10 , C12N15/02 , C12N15/09 , C12P21/08 , G01N33/569 , G01N33/68 , A61K39/395 , A61K49/00 , C07K16/00
CPC分类号： C07K16/18 , G01N33/56983 , G01N33/6896 , C07K2317/55 , G01N2800/2828
摘要： Antibodies are disclosed which specifically bind to native PrP.sup.Sc in situ. Preferred antibodies bind only to the native PrP.sup.Sc of a particular species e.g., human, cow, sheep, pig, etc. Particularly preferred antibodies bind specifically to a particular isoform of human PrP.sup.Sc. Preferred antibodies of the invention are (1) produced by phage display methodology, (2) bind specifically to native PrP.sup.Sc, (3) neutralizes the infectivity of prions, (4) bind to PrP.sup.Sc in situ and (5) bind 50% or more of PrP.sup.Sc in a liquid flowable sample. Antibodies of the invention can be bound to a substrate and used to assay a sample (which has any PrP.sup.c denatured via proteinase K) for the presence of PrP.sup.Sc of a specific species which PrP.sup.Sc is associated with disease. Antibodies which specifically bind to human PrP.sup.Sc can be labeled and injected carrying out an in vivo diagnostic test to determine if the human is infected with prions associated with disease. The antibodies are preferably produced using phage display technology wherein the genetic material in the phage expressing the antibody is obtained from a mammal with an ablated endogenous PrP protein gene and an endogenous chimeric PrP gene which mammal had been inoculated with PrP.sup.Sc to induce antibody production.

9. 发明授权

US06620629B1 Method for detecting prions 失效
标题翻译：检测朊病毒的方法
公开(公告)号：US06620629B1
公开(公告)日：2003-09-16
申请号：US09699033
申请日：2000-10-27
申请人： Stanley B. Prusiner , Jiri Safar
发明人： Stanley B. Prusiner , Jiri Safar
IPC分类号： G01N33531
CPC分类号： G01N33/6896 , G01N2800/2828
摘要： The present invention provides assays for identifying the levels of both protease sensitive and protease resistant conformers of PrPSc in a sample. In a preferred embodiment, the assay comprises determining levels of total PrPSc in a sample, subjecting the PrPSc fraction to treatment with a protease that selectively hydrolyzes the protease sensitive PrPSc (sPrPSc) conformers, and quantifying the levels of sPrPSc in the sample. The ability to detect sPrPSc allows early detection of prions, since the PrPSc in easily accessible biological samples such as blood is predominantly sPrPSc. The ratio of sPrPSc to rPrPSc also allows the identification of a particular prion strain in an infected sample.
摘要翻译：本发明提供用于鉴定样品中PrPSc的蛋白酶敏感性和蛋白酶抗性构象异构体的水平的测定。在优选的实施方案中，测定法包括测定样品中总PrPSc的水平，使PrPSc部分用选择性水解蛋白酶敏感性PrPSc（sPrPSc）构象异构体的蛋白酶进行处理，并量化样品中sPrPSc的水平。检测sPrPSc的能力允许早期检测朊病毒，因为容易获得的生物样品（如血液）中的PrPSc主要是sPrPSc。 sPrPSc与rPrPSc的比值也允许鉴定感染样品中特定的朊病毒株。

10. 发明申请

US20140287957A1 ISOTOPIC LABELING FOR THE MEASUREMENT OF GLOBAL PROTEIN LEVELS AND TURNOVER IN VIVO 有权
标题翻译：用于测量全球蛋白质水平和转基因生物体内的同位素标记
公开(公告)号：US20140287957A1
公开(公告)日：2014-09-25
申请号：US13704498
申请日：2011-06-17
申请人： Stanley B. Prusiner , John C. Price , Sina Ghaemmaghami , Al Burlingame , Shenheng Guan
发明人： Stanley B. Prusiner , John C. Price , Sina Ghaemmaghami , Al Burlingame , Shenheng Guan
IPC分类号： G01N33/68 , G01N33/50
CPC分类号： G01N33/5088 , G01N33/6842 , G01N33/6848
摘要： An entire complement or plurality of isotopically labeled amino acids are introduced into the diet of a test subject. Sufficient amounts of the isotopically labeled amino acids are provided to the subject in order to ensure that the subject incorporates a large percentage of isotopically labeled amino acids into newly synthesized proteins. Tissue samples are removed from the subject at different points in time and proteins are extracted and separated so that different proteins of different tissues can be individually analyzed and their amount and pattern of isotopic labeling can be determined. In a preferred embodiment, the methodology can be combined with proteolytic digestion to peptides and analysis by mass spectrometry in order to measure rates of protein turnover in vivo relating to thousands of different proteins.
摘要翻译：将整个补体或多个同位素标记的氨基酸引入测试对象的饮食中。向受试者提供足够量的同位素标记的氨基酸，以确保受试者将大量同位素标记的氨基酸掺入新合成的蛋白质中。在不同的时间点从受试者中取出组织样品，提取和分离蛋白质，以便不同组织的不同蛋白质可以单独分析，并且可以测定其同位素标记的数量和模式。在优选的实施方案中，该方法可以与肽的蛋白水解消化和质谱分析相结合，以便测量与数千种不同蛋白质相关的体内蛋白质周转率。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式