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    • 5. 发明申请
    • PROTEIN HAVING SWEETNESS
    • 有蛋白质的蛋白质
    • US20100040754A1
    • 2010-02-18
    • US12532716
    • 2008-01-28
    • Keiko AbeKenichiro NakajimaTomiko AsakuraTakumi MisakaSho Ishii
    • Keiko AbeKenichiro NakajimaTomiko AsakuraTakumi MisakaSho Ishii
    • C12N5/10C07K14/00C12N15/11C12N15/00A23L1/236
    • C07K14/415A23L27/31C07K14/43
    • An object of the present invention is to discover a substance with sweetness independent of pH and to provide a novel composition with sweetness characterized by containing the substance. Provided are: an NAS variant characterized by having sweetness independent of pH in the case of combination with a polypeptide NBS variant by causing substitution mutation or deletion mutation in one or several amino acids out of the amino acid residues constituting a polypeptide NAS; an NBS variant characterized by having sweetness independent of pH in the case of combination with a polypeptide NAS variant by causing substitution mutation or deletion mutation in one or several amino acids out of the amino acid residues constituting a polypeptide NBS; a diner comprising a combination of the NAS variant and the NBS variant; a recombinant vector containing a gene encoding the NAS variant and a gene encoding the NBS variant; and a transformant containing the vector.
    • 本发明的目的是发现与pH无关的甜味物质,并提供具有特征在于含有该物质的甜味的新型组合物。 提供:NAS变体,其特征在于通过在构成多肽NAS的氨基酸残基中的一个或几个氨基酸中引起取代突变或缺失突变,在与多肽NBS变体组合的情况下具有与pH无关的甜度; 在通过在构成多肽NBS的氨基酸残基中的一个或几个氨基酸中引发取代突变或缺失突变而与多肽NAS变体组合的情况下,其特征在于具有独立于pH的甜度的NBS变体; 包括NAS变体和NBS变体的组合的餐具; 包含编码NAS变体的基因和编码NBS变体的基因的重组载体; 和含有载体的转化体。