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1. 发明申请

US20080138904A1 Method Of Breeding Cells To Improve Tolerance To Short Chain Fatty Acids 审中-公开
标题翻译：育种细胞方法提高短链脂肪酸的耐受性
公开(公告)号：US20080138904A1
公开(公告)日：2008-06-12
申请号：US11793145
申请日：2005-12-12
申请人： Shigeru Nakano , Masahiro Fukaya
发明人： Shigeru Nakano , Masahiro Fukaya
IPC分类号： C12N15/09 , C12N1/21
CPC分类号： C12P7/40 , C12N1/00 , C12N15/52 , C12P7/52 , C12P7/54
摘要： It is intended to provide a method of conferring to microbial cells or the like, tolerance to a short chain fatty acid, including formic acid and acetic acid, which is harmful to the growth thereof without suppressing the growth of the cells while maintaining a high productivity of useful substances. It is also intended to provide a means for efficiently culturing a microorganism in the presence of a short chain fatty acid, or a means for producing a useful short chain fatty acid via fermentation.The present invention provides a method of breeding cells to improve tolerance to a short chain fatty acid, wherein a gene encoding a protein having a function of transporting a short chain fatty acid from the inside of cells to the outside of cells, is transformed into and expressed in the cells; a high cell-density culture method for producing useful substances using the cell; and a method of preparing a fermentation broth comprising a short chain fatty acid, wherein the cells are cultured under the conditions such that the cells produce a short chain fatty acid.
摘要翻译：旨在提供赋予微生物细胞等的方法，对包含甲酸和乙酸的短链脂肪酸的耐受性，其对生长有害，而不抑制细胞生长，同时保持高生产率的有用物质。还提供了在短链脂肪酸存在下有效培养微生物的方法，或者是通过发酵生产有用的短链脂肪酸的方法。本发明提供一种育种细胞以改善对短链脂肪酸耐受性的方法，其中将编码具有从细胞内部输送到外部的短链脂肪酸的功能的蛋白质的基因转化到细胞外，在细胞中表达; 用于使用该细胞产生有用物质的高细胞密度培养方法; 以及制备包含短链脂肪酸的发酵液的方法，其中所述细胞在使细胞产生短链脂肪酸的条件下培养。

2. 发明申请

US20130220270A1 RETROFIT GAS FUEL SUPPLY KIT RETROFITTABLE TO INTERNAL COMBUSTION ENGINE USING LIQUID FUEL 审中-公开
标题翻译：改装燃油供应箱可以使用液体燃料改装成内燃机
公开(公告)号：US20130220270A1
公开(公告)日：2013-08-29
申请号：US13698961
申请日：2011-05-23
申请人： Sakuichirou Imai , Shigeru Nakano
发明人： Sakuichirou Imai , Shigeru Nakano
IPC分类号： F02B17/00
CPC分类号： F02B17/005 , F02B3/06 , F02B2075/125 , F02B2275/16 , F02D19/061 , F02D19/0644 , F02D19/066 , F02D19/0689 , F02D19/0692 , F02D19/081 , F02D41/0025 , F02D41/0027 , F02D41/266 , F02D2041/389 , F02D2400/11 , F02M21/0281 , F02M35/10085 , F02M35/112 , Y02T10/123 , Y02T10/32 , Y02T10/36
摘要： The invention provides a gas fuel supply kit for the existing vehicle (vehicle mounting a gasoline engine, or a diesel engine), in order to use a gas fuel such as hydrogen after wards.A post-installable type gas fuel supply kit to be installed in the existing vehicle such as a gasoline vehicle, comprising a gas fuel injector for supplying the gas fuel such as hydrogen to an intake path; an injector installing means for installing said gas fuel injector to a location which is a different location where a gasoline injector is installed; and a control unit for controlling at least an injection amount, and an injection timing of the gas fuel at the hydrogen fuel injector.
摘要翻译：本发明提供了一种用于现有车辆（车辆安装汽油发动机或柴油发动机）的气体燃料供应套件，以便在病房之后使用气体燃料例如氢气。一种可安装在诸如汽油车辆的现有车辆中的可安装后的气体燃料供应套件，包括用于将气体燃料（例如氢气）供应到进气通道的气体燃料喷射器; 喷射器安装装置，用于将所述气体燃料喷射器安装到安装有汽油喷射器的不同位置的位置; 以及控制单元，用于至少控制在氢燃料喷射器处的气体燃料的喷射量和喷射正时。

3. 发明申请

US20100209566A1 METHOD OF SEPARATING AND DISTINGUISHING WALNUT FROM PECAN NUT 有权
标题翻译：从PECAN NUT分离和分离WALNUT的方法
公开(公告)号：US20100209566A1
公开(公告)日：2010-08-19
申请号：US12524735
申请日：2008-01-30
申请人： Takeo Yano , Yumiko Sakai , Kouji Uchida , Yoshiki Nakao , Kimie Ishihata , Shigeru Nakano , Toshihiro Yamada
发明人： Takeo Yano , Yumiko Sakai , Kouji Uchida , Yoshiki Nakao , Kimie Ishihata , Shigeru Nakano , Toshihiro Yamada
IPC分类号： G01N33/02
CPC分类号： C12Q1/6895 , A01H1/04 , C07K14/415
摘要： [Object] To newly develop a method whereby walnut can be simply, rapidly and accurately separated and distinguished from pecan nut.[Means for Resolution] Due to the increase in the number of patients with the walnut allergen and the increase of import of pecan nut which is an edible nut belonging to the family Juglandaceae, recently in Japan, the development of a method for detecting walnut and the nut in a discriminative manner is urgently needed. With attention focused on the chloroplast matK gene with the apparent gene sequence, we developed a PCR method for specifically detecting nuts of the family Juglandaceae and identified the matK sequence of pecan nut to establish a method for discriminating walnut and pecan nut from each other using restriction enzymes, for discriminating walnut and pecan nut from each other.
摘要翻译： [对象]新开发一种核桃可以简单，快速，准确地分离和区别于山核桃坚果的方法。 [解决方法]由于核桃过敏原患者数量的增加以及最近在日本属于菊科植物的可食用坚果的山核桃进口量的增加，开发了检测核桃的方法迫切需要有歧视的手段。对于叶绿体matK基因与表观基因序列的关注，我们开发了一种特异性检测菊科植物坚果的PCR方法，并确定了山核桃坚果的matK序列，建立了一种用核桃和山核桃相互鉴别核桃和山核桃的方法用于区分核桃和山核桃果仁的酶。

4. 发明授权

US07354751B2 Alcohol dehydrogenase gene of acetic acid bacterium 有权
标题翻译：乙酸细菌的酒精脱氢酶基因
公开(公告)号：US07354751B2
公开(公告)日：2008-04-08
申请号：US10548486
申请日：2004-02-17
申请人： Shigeru Nakano
发明人： Shigeru Nakano
IPC分类号： C07H21/04 , C12P21/06 , C12N9/02 , C12N1/21
CPC分类号： C12N9/0006 , C12J1/04 , C12P7/54
摘要： A novel gene having a function of improving acetic acid fermentation in practical level was cloned from a practical acetic acid bacterium belonging to the genus Gluconacetobacter by a method for obtaining a gene having a growth-promoting function in acetic acid-containing medium from the chromosomal DNA library of the acetic acid bacterium. It was made possible to significantly shorten the growth induction period and significantly improve the acetic acid fermentation rate of transformants obtained by introducing the gene into acetic acid bacteria, when such transformants are cultured in the presence of ethanol.
摘要翻译：通过从染色体DNA获得含有乙酸的培养基中具有生长促进功能的基因的方法，从属于葡糖型醋杆菌属的实用乙酸菌克隆了具有实用水平的乙酸发酵功能的新型基因醋酸菌库。通过在乙醇存在下培养这些转化体，可以显着缩短生长诱导期，显着提高通过将基因导入乙酸菌而得到的转化体的乙酸发酵率。

5. 发明授权

US06892545B2 Automatic refrigerator system, refrigerator, automatic cooking system, and microwave oven 有权
标题翻译：自动冰箱系统，冰箱，自动烹饪系统和微波炉
公开(公告)号：US06892545B2
公开(公告)日：2005-05-17
申请号：US09959460
申请日：2001-02-28
申请人： Toshiharu Ishikawa , Shigeru Nakano
发明人： Toshiharu Ishikawa , Shigeru Nakano
IPC分类号： B65D5/42 , B65D23/14 , B65D77/24 , F25D23/02 , F25D25/00 , F25D29/00 , G06Q10/00 , H05B6/68 , F25D23/00 , G06K19/07
CPC分类号： H05B6/6441 , B65D5/42 , B65D5/4233 , B65D23/14 , B65D77/24 , B65D2203/10 , F25B2600/07 , F25D23/028 , F25D25/00 , F25D29/005 , F25D2400/36 , F25D2700/08 , G06Q10/087
摘要： An automatic cold-storage system includes a food package (22) provided with a noncontact IC tag (11), and a refrigerator (31) for storing the food package (22). Information is read from the noncontact IC tag (11) by a noncontact IC tag reader (12) and is compiled to a database (31c). A managing means (31b) controls the refrigerator (31) on the basis of information contained in the database (31c). The managing means (31b) makes a display device (14) display a warning on the basis of information read by the noncontact IC tag reader (12).
摘要翻译：一种自动冷藏系统，包括设置有非接触IC标签（11）的食品包装（22）和用于储存食品包装（22）的冰箱（31）。通过非接触IC标签读取器（12）从非接触IC标签（11）读取信息，并将其编译到数据库（31c）。管理装置（31a）基于包含在数据库（31c）中的信息来控制冰箱（31）。管理装置（31b）使得显示装置（14）基于由非接触IC标签读取器（12）读取的信息来显示警告。

6. 发明授权

US5853806A Process for hot dip-coating steel material with molten aluminum alloy by one-stage coating method using flux and bath of molten aluminum alloy metal 失效
标题翻译：使用熔融铝合金金属助熔剂和浴液，通过一步涂布法将熔融铝合金热浸涂钢材的工艺
公开(公告)号：US5853806A
公开(公告)日：1998-12-29
申请号：US822762
申请日：1997-03-24
申请人： Seijun Higuchi , Shigeru Nakano , Toshiyuki Aishima
发明人： Seijun Higuchi , Shigeru Nakano , Toshiyuki Aishima
IPC分类号： C23C2/02 , C23C2/12 , C23C2/30 , B05D3/04
CPC分类号： C23C2/02 , C23C2/30
摘要： Disclosed are a process for hot-dip coating a steel material with a molten aluminum alloy according to a one-stage coating method using a flux which comprises removing an oxide layer on a steel material surface, conducting activating treatment, then coating a chloride flux solution and dipping the steel material in a molten aluminum alloy coating bath floated a fluoride-containing flux, thereby coating the steel material surface with aluminum alloy, or comprises removing an oxide layer on a steel material surface, adjusting surface roughness, conducting activating treatment and dipping the steel material in a molten Al--Zn--Si alloy or Al--Si alloy coating bath floated a flux added an iron component, thereby coating the steel material surface with Al--Zn--Si alloy or Al--Si alloy to adhere a large amount thereof and a aluminum alloy coating bath.
摘要翻译：本发明公开了一种使用熔融金属的一次涂覆方法，使用熔融铝合金热浸涂覆钢材的方法，该焊剂包括除去钢材表面上的氧化物层，进行活化处理，然后涂覆氯化物助熔剂溶液将钢材浸入熔融铝合金涂层中漂浮含氟化物的焊剂，由此用铝合金涂覆钢材表面，或者除去钢材表面的氧化物层，调整表面粗糙度，进行活化处理和浸渍在熔融的Al-Zn-Si合金或Al-Si合金涂层中的钢材漂浮加入铁组分的助熔剂，由此用Al-Zn-Si合金或Al-Si合金涂覆钢材表面以大量粘附和铝合金涂层浴。

7. 发明授权

US07446192B2 Gene participating in acetic acid tolerance, acetic acid bacteria bred using the gene, and process for producing vinegar with the use of the acetic acid bacteria 有权
标题翻译：基因参与乙酸耐量，使用该基因繁殖的乙酸菌，以及使用乙酸菌生产醋的方法
公开(公告)号：US07446192B2
公开(公告)日：2008-11-04
申请号：US10507446
申请日：2003-03-12
申请人： Hidetsugu Goto , Shigeru Nakano
发明人： Hidetsugu Goto , Shigeru Nakano
IPC分类号： C07H21/04
CPC分类号： C12J1/04 , C07K14/195 , C12N1/20
摘要： The present invention is intended to provide novel genes participating in acetic acid tolerance of acetic acid bacteria, and a method of improving acetic acid tolerance of microorganisms, particularly that of acetic acid bacteria by using the genes, further a method of efficiently producing vinegar with acetic acid at higher concentration by using acetic acid bacteria whose acetic acid tolerance is improved. In the present invention, novel genes having a function for improving acetic acid tolerance on practical level were cloned from practical acetic acid bacteria belonging to the genus Gluconacetobacter by a method of obtaining genes from chromosomal DNA library that enable to grow on the medium at a high concentration of acetic acid. Further, in transformants in which the genes were introduced into acetic acid bacteria, acetic acid tolerance was remarkably increased, and when the transformants are subjected to aeration culture in the presence of ethanol, the growth lag-time can be shortened, and the growth rate can also be improved, moreover the final acetic acid concentration can be remarkably improved.
摘要翻译：本发明旨在提供参与乙酸菌的乙酸耐受性的新基因，以及通过使用该基因来提高微生物，特别是乙酸菌的乙酸耐受性的方法，还提出了一种有效生产乙酸醋的方法通过使用醋酸耐受性提高的乙酸菌，浓度较高的酸。在本发明中，通过从染色体DNA文库获得能够在培养基上高生长的基因的方法，从属于葡糖型醋杆菌属的实用乙酸菌克隆了具有实用水平提高乙酸耐受性的新型基因乙酸浓度。此外，在将基因导入乙酸菌的转化体中，乙酸耐受性显着增加，当转化体在乙醇存在下进行通气培养时，可以缩短生长滞后时间，生长速度也可以改善，而且可以显着提高最终的乙酸浓度。

8. 发明申请

US20070088088A1 Method for preventing or treating metabolic syndrome 审中-公开
标题翻译：预防或治疗代谢综合征的方法
公开(公告)号：US20070088088A1
公开(公告)日：2007-04-19
申请号：US11406339
申请日：2006-04-19
申请人： Yoichi Inada , Shigeru Nakano , Hiroaki Masuzaki
发明人： Yoichi Inada , Shigeru Nakano , Hiroaki Masuzaki
IPC分类号： A61K31/192
CPC分类号： A61K31/192
摘要： A method for preventing or treating metabolic syndrome by administering bezafibrate. Since bezafibrate suppresses the action of 11β-hydroxysteroid dehydrogenase type 1 and also accelerates expression of adiponectin receptor, it is used as an agent for preventing or treating metabolic syndrome.
摘要翻译：一种通过施用苯扎贝特来预防或治疗代谢综合征的方法。由于苯扎贝特抑制了1型11β-羟类固醇脱氢酶的作用，并且加速了脂连蛋白受体的表达，因此用作预防或治疗代谢综合征的药剂。

9. 发明申请

US20060228445A1 Gene participating in growth promoting function of acetic acid bacterium and utilization of the same 有权
标题翻译：基因参与醋酸菌的生长促进功能及其利用
公开(公告)号：US20060228445A1
公开(公告)日：2006-10-12
申请号：US10561834
申请日：2004-06-16
申请人： Shigeru Nakano
发明人： Shigeru Nakano
IPC分类号： C12J1/00 , C07H21/04 , C12P21/06 , C12N9/02 , C12N15/74 , C12N1/21
CPC分类号： C07K14/195 , C12J1/00 , C12J1/04
摘要： The present invention provides a novel gene involved in the growth-promoting function of acetic acid bacteria and a microorganism containing the gene. In particular, the present invention provides a method for enhancing the growth-promoting function of acetic acid bacteria and a method for efficiently producing vinegar containing acetic acid at a high concentration in a short time using such acetic acid bacteria having an enhanced growth-promoting function.
摘要翻译：本发明提供了一种涉及乙酸细菌生长促进功能的新基因和含有该基因的微生物。特别地，本发明提供了一种提高乙酸菌生长促进功能的方法以及使用具有增强生长促进功能的乙酸菌在短时间内高效浓缩高浓度乙酸醋的方法。

10. 发明授权

US07096221B2 Food information management system 有权
标题翻译：食品信息管理系统
公开(公告)号：US07096221B2
公开(公告)日：2006-08-22
申请号：US09901595
申请日：2001-07-11
申请人： Shigeru Nakano
发明人： Shigeru Nakano
IPC分类号： G06F17/30 , G06F7/06
CPC分类号： G06F19/3475 , F25D2700/08 , G06F19/00 , G06F19/324 , Y10S707/99945
摘要： A food information management system includes a food database (11) containing food cooking conditions for cooking foods, and nutritive ingredients, energy-producing values and weight of foods, and information storage mediums (20, 20a). Each information storage medium is attached to a food package (21a) containing a processed food (21) and storing food information including food ID information specifying a food. When cooling the processed food (21), the food ID information stored in the food information storage medium (20, 20a) is read by a read means. Food information about a food specified by the food ID information is retrieved from the food database (11), an output means receives the food information, and a display means displays the food information.
摘要翻译：食品信息管理系统包括食物数据库（11），其包含用于烹饪食物的食物烹饪条件，以及营养成分，能量产生值和食物重量以及信息存储介质（20,20a）。每个信息存储介质连接到包含加工食品（21）的食品包装（21a），并存储包括食物的食品信息，食品信息包括食物ID信息。当冷却加工食物（21）时，通过读取装置读取存储在食物信息存储介质（20,20a）中的食物ID信息。从食品数据库（11）检索关于由食品ID信息指定的食物的食物信息，输出装置接收食品信息，并且显示装置显示食品信息。

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