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1. 发明授权

US07629139B2 Extraction methods and assays for feed enzymes 有权
标题翻译：饲料酶的提取方法和测定
公开(公告)号：US07629139B2
公开(公告)日：2009-12-08
申请号：US11455954
申请日：2006-06-20
申请人： Shib Sankar Basu , Stephanie Winslow , Andrea Nelson , Makoto Ono , Scott Betts
发明人： Shib Sankar Basu , Stephanie Winslow , Andrea Nelson , Makoto Ono , Scott Betts
IPC分类号： C12Q1/34 , G01N1/18
CPC分类号： C12Q1/40 , C12Q1/34 , C12Q1/42 , Y10T436/255
摘要： This invention relates to methods of the detection of enzymatically active enzymes in feed added in the pre-pelleting stage. Further, this invention relates to the field of detecting thermotolerant enzymes added to feed in the pre-pelleting stage. In particular, the invention relates to the detection of a phytase enzyme, in particular an E. coli phytase or an E. coli derived phytase in feed, such as Quantum™ Phytase.
摘要翻译：本发明涉及在预颗粒阶段加入的饲料中检测酶活性酶的方法。此外，本发明涉及检测在预制粒阶段中加入饲料中的耐热酶的领域。特别地，本发明涉及植酸酶，特别是饲料中的大肠杆菌植酸酶或大肠杆菌衍生的植酸酶的检测，例如Quantum TM植酸酶。

2. 发明授权

US08497127B2 Engineering enzymatically susceptible proteins 有权
标题翻译：工程酶易感蛋白
公开(公告)号：US08497127B2
公开(公告)日：2013-07-30
申请号：US12743418
申请日：2008-11-21
申请人： Shib Sankar Basu , Shengsheng Zhang
发明人： Shib Sankar Basu , Shengsheng Zhang
IPC分类号： C12N15/00
CPC分类号： A23K20/189 , A23K50/75 , C12N9/16
摘要： The invention provides a synthetic phytase polypeptide which encodes an enzymatically susceptible phytase. Also provided are feed or food products comprising an enzymatically susceptible phytase, and transgenic plants which express the enzymatically susceptible phytase. Further provided are methods for making and using enzymatically susceptible phytases, e.g., a method of using an enzymatically susceptible phytase in feed and food processing.
摘要翻译：本发明提供编码酶易感的植酸酶的合成植酸酶多肽。还提供了包含酶易感的植酸酶的饲料或食品，以及表达酶易感的植酸酶的转基因植物。进一步提供了制备和使用酶敏感性植酸酶的方法，例如在饲料和食品加工中使用酶促敏感的植酸酶的方法。

3. 发明申请

US20130084641A1 Compositions and Methods for Protein Production 有权
标题翻译：蛋白质生产的组成和方法
公开(公告)号：US20130084641A1
公开(公告)日：2013-04-04
申请号：US13708479
申请日：2012-12-07
申请人： Shib Sankar Basu , Kristern Kamerath Dang , Kasimalai Azhakanandam , Thomas Z. McNeill , Sheng Guo
发明人： Shib Sankar Basu , Kristern Kamerath Dang , Kasimalai Azhakanandam , Thomas Z. McNeill , Sheng Guo
IPC分类号： C12N15/10
CPC分类号： C12N15/1089 , C12N15/1034
摘要： The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.
摘要翻译：本发明提供了选择产生感兴趣多肽的mRNA的方法，包括：a）产生包含编码目的多肽的不同核苷酸序列的单个mRNA序列的阵列; b）确定（a）的每个单独mRNA序列的一个或多个或两个或更多个以下参数：（i）最小自由能（MFE）RNA二级结构; （ii）合奏自由能（EFE）; （iii）热力学集合中最小自由能（FMFE）RNA二级结构的频率; 和（iv）集体多样性（ED）; c）根据步骤（b）中确定的参数对阵列的各个mRNA序列进行排序; 和d）从步骤（c）的排列阵列中选择mRNA序列，其中所选择的mRNA产生感兴趣的多肽。本发明还提供了选择用于增强和减少感兴趣的多肽的产生的mRNA的方法。

4. 发明申请

US20120080590A1 METHODS OF DETERMINING PLANT ZYGOSITY USING MASS SPECTROMETRY 审中-公开
标题翻译：使用质谱分析方法确定植物的ZYGOSITY
公开(公告)号：US20120080590A1
公开(公告)日：2012-04-05
申请号：US12897273
申请日：2010-10-04
申请人： Shib Sankar Basu
发明人： Shib Sankar Basu
IPC分类号： H01J49/00
CPC分类号： G01N33/6848 , A01H1/04 , G01N30/7233 , G01N2333/415
摘要： The present invention relates to methods and systems for identifying seeds that are homozygous for an allele of interest and/or for identifying seeds that are likely to produce plants possessing a desired trait. Methods of producing plants that are homozygous for an allele of interest and/or that possess a desired trait are also provided.
摘要翻译：本发明涉及用于鉴定对于感兴趣的等位基因是纯合的种子和/或鉴定可能产生具有所需性状的植物的种子的方法和系统。还提供了产生对目的等位基因是纯合的植物和/或具有所需性状的植物的方法。

5. 发明授权

US07919681B2 Self-processing plants and plant parts 有权
标题翻译：自加工厂和厂房部件
公开(公告)号：US07919681B2
公开(公告)日：2011-04-05
申请号：US11874560
申请日：2007-10-18
申请人： Michael B. Lanahan , Shib Sankar Basu , Christopher J. Batie , Wen Chen , Joyce Craig , Mark Kinkema
发明人： Michael B. Lanahan , Shib Sankar Basu , Christopher J. Batie , Wen Chen , Joyce Craig , Mark Kinkema
IPC分类号： C12N15/84 , C12N15/56 , C12N15/62 , C12P7/06 , C12P19/00
CPC分类号： C12N9/2428 , C12N9/2408 , C12N9/2411 , C12N9/2414 , C12N9/2422 , C12N9/2451 , C12N9/2457 , C12N9/246 , C12N15/8245 , C12P7/06 , C12P19/14 , C12Y302/01001 , C12Y302/01003 , C12Y302/0102 , C12Y302/01041 , C12Y302/01068 , Y02E50/16 , Y02E50/17
摘要： The invention provides polynucleotides, preferably synthetic polynucleotides, which encode processing enzymes that are optimized for expression in plants. The polynucleotides encode mesophilic, thermophilic, or hyperthermophilic processing enzymes, which are activated under suitable activating conditions to act upon the desired substrate. Also provided are “self-processing” transgenic plants, and plant parts, e.g., grain, which express one or more of these enzymes and have an altered composition that facilitates plant and grain processing. Methods for making and using these plants, e.g., to produce food products having improved taste and to produce fermentable substrates for the production of ethanol and fermented beverages are also provided.
摘要翻译：本发明提供了多核苷酸，优选合成的多核苷酸，其编码为在植物中表达而优化的加工酶。多核苷酸编码嗜温，嗜热或超嗜热加工酶，其在合适的活化条件下活化以作用于所需的底物。还提供了“自加工”转基因植物和植物部分，例如表达这些酶中的一种或多种并且具有改变的组合物的植物和谷粒加工的植物部分。还提供了制备和使用这些植物的方法，例如生产具有改善味道的食品并生产用于生产乙醇和发酵饮料的可发酵底物。

6. 发明申请

US20110041221A1 ENGINEERING ENZYMATICALLY SUSCEPTIBLE PHYTASES 有权
标题翻译：工程化学可靠的植物
公开(公告)号：US20110041221A1
公开(公告)日：2011-02-17
申请号：US12744483
申请日：2008-11-21
申请人： Shib Sankar Basu , Shengsheng Zhang
发明人： Shib Sankar Basu , Shengsheng Zhang
IPC分类号： A01H5/00 , C12N9/16 , C12N15/55 , C12N15/63 , C12N1/21 , C12N1/19 , C12N1/15 , C12N5/07 , C12N5/04 , A01H5/10
CPC分类号： A23K20/189 , A23K50/75 , C12N9/16
摘要： The invention provides a synthetic phytase polypeptide which encodes an en/ymatically susceptible phytase. Also provided are feed or food products comprising an enzymatically susceptible phytase, and transgenic plants which express the enzymatically susceptible phytase. Further provided are methods for making and using enzymatically susceptible phytases, e.g., a method of using an enzymatically susceptible phytase in feed and food processing.
摘要翻译：本发明提供了编码植酸酶敏感的植酸酶的合成植酸酶多肽。还提供了包含酶易感的植酸酶的饲料或食品，以及表达酶易感的植酸酶的转基因植物。进一步提供了制备和使用酶敏感性植酸酶的方法，例如在饲料和食品加工中使用酶促敏感的植酸酶的方法。

7. 发明授权

US07557262B2 Self processing plants and plant parts 有权
标题翻译：自加工厂和厂房部件
公开(公告)号：US07557262B2
公开(公告)日：2009-07-07
申请号：US11415818
申请日：2006-05-02
申请人： Michael B. Lanahan , Shib Sankar Basu , Christopher J. Batie , Wen Chen , Joyce Craig , Mark Kinkema
发明人： Michael B. Lanahan , Shib Sankar Basu , Christopher J. Batie , Wen Chen , Joyce Craig , Mark Kinkema
IPC分类号： C12N15/82 , C12N15/56 , C12N15/62 , A01H5/00 , A01H5/10
CPC分类号： C12N9/2428 , C12N9/2408 , C12N9/2411 , C12N9/2414 , C12N9/2422 , C12N9/2451 , C12N9/2457 , C12N9/246 , C12N15/8245 , C12P7/06 , C12P19/14 , C12Y302/01001 , C12Y302/01003 , C12Y302/0102 , C12Y302/01041 , C12Y302/01068 , Y02E50/16 , Y02E50/17
摘要： The invention provides polynucleotides, preferably synthetic polynucleotides, which encode processing enzymes that are optimized for expression in plants. The polynucleotides encode mesophilic, thermophilic, or hyperthermophilic processing enzymes, which are activated under suitable activating conditions to act upon the desired substrate. Also provided are “self-processing” transgenic plants, and plant parts, e.g., grain, which express one or more of these enzymes and have an altered composition that facilitates plant and grain processing. Methods for making and using these plants, e.g., to produce food products having improved taste and to produce fermentable substrates for the production of ethanol and fermented beverages are also provided.
摘要翻译：本发明提供了多核苷酸，优选合成的多核苷酸，其编码为在植物中表达而优化的加工酶。多核苷酸编码嗜温，嗜热或超嗜热加工酶，其在合适的活化条件下活化以作用于所需的底物。还提供了“自加工”转基因植物和植物部分，例如表达这些酶中的一种或多种并且具有改变的组合物的植物和谷粒加工的植物部分。还提供了制备和使用这些植物的方法，例如生产具有改善味道的食品并生产用于生产乙醇和发酵饮料的可发酵底物。

8. 发明授权

US08409641B2 Engineering enzymatically susceptible phytases 有权
标题翻译：工程酶易感植酸酶
公开(公告)号：US08409641B2
公开(公告)日：2013-04-02
申请号：US12744483
申请日：2008-11-21
申请人： Shib Sankar Basu , Shengsheng Zhang
发明人： Shib Sankar Basu , Shengsheng Zhang
IPC分类号： A23K1/165 , C12N9/16 , A61K38/46
CPC分类号： A23K20/189 , A23K50/75 , C12N9/16
摘要： The invention provides a synthetic phytase polypeptide which encodes an en/ymatically susceptible phytase. Also provided are feed or food products comprising an enzymatically susceptible phytase, and transgenic plants which express the enzymatically susceptible phytase. Further provided are methods for making and using enzymatically susceptible phytases, e.g., a method of using an enzymatically susceptible phytase in feed and food processing.
摘要翻译：本发明提供了编码植酸酶敏感的植酸酶的合成植酸酶多肽。还提供了包含酶易感的植酸酶的饲料或食品，以及表达酶易感的植酸酶的转基因植物。进一步提供了制备和使用酶敏感性植酸酶的方法，例如在饲料和食品加工中使用酶促敏感的植酸酶的方法。

9. 发明申请

US20100240082A1 METHODS FOR DETECTING AND MEASURING POLYSACCHARIDE-HYDROLYZING ENZYMES 审中-公开
标题翻译：检测和测量多糖 - 水解酶的方法
公开(公告)号：US20100240082A1
公开(公告)日：2010-09-23
申请号：US12726424
申请日：2010-03-18
申请人： Tricia Moser , Shib Sankar Basu , Rogerio T.N. Prata , Hangsik Moon , Xuejun Zhong , Myoung Kim
发明人： Tricia Moser , Shib Sankar Basu , Rogerio T.N. Prata , Hangsik Moon , Xuejun Zhong , Myoung Kim
IPC分类号： C12Q1/40 , C12Q1/34
CPC分类号： C12Q1/34 , C12Q1/40 , G01N2333/924 , G01N2333/928 , G01N2333/942 , G01N2333/944
摘要： Methods are disclosed for detecting and measuring polysaccharide-hydrolyzing enzyme activity or concentration by partial hydrolysis using a pre-determined, yet short, incubation time and a pre-determined temperature. The resulting reaction mixture has unique chemical (i.e., reaction products) and physical (i.e., viscosity) properties that can be used to detect or measure the polysaccharide-hydrolyzing enzyme activity or concentration.
摘要翻译：公开了通过使用预定的但短的孵育时间和预定温度通过部分水解来检测和测量多糖水解酶活性或浓度的方法。所得反应混合物具有独特的化学（即反应产物）和物理（即粘度）性质，可用于检测或测量多糖水解酶活性或浓度。

10. 发明申请

US20090288232A1 SELF PROCESSING PLANTS AND PLANT PARTS 有权
标题翻译：自我处理植物和植物部分
公开(公告)号：US20090288232A1
公开(公告)日：2009-11-19
申请号：US12471564
申请日：2009-05-26
申请人： Michael B. Lanahan , Shib Sankar Basu , Christopher J. Batie , Wen Chen , Joyce Craig , Mark Kinkema
发明人： Michael B. Lanahan , Shib Sankar Basu , Christopher J. Batie , Wen Chen , Joyce Craig , Mark Kinkema
IPC分类号： A01H5/00 , C12N15/82 , C12N5/10
CPC分类号： C12N9/2428 , C12N9/2408 , C12N9/2411 , C12N9/2414 , C12N9/2422 , C12N9/2451 , C12N9/2457 , C12N9/246 , C12N15/8245 , C12P7/06 , C12P19/14 , C12Y302/01001 , C12Y302/01003 , C12Y302/0102 , C12Y302/01041 , C12Y302/01068 , Y02E50/16 , Y02E50/17
摘要： The invention provides polynucleotides, preferably synthetic polynucleotides, which encode processing enzymes that are optimized for expression in plants. The polynucleotides encode mesophilic, thermophilic, or hyperthermophilic processing enzymes, which are activated under suitable activating conditions to act upon the desired substrate. Also provided are “self-processing” transgenic plants, and plant parts, e.g., grain, which express one or more of these enzymes and have an altered composition that facilitates plant and grain processing. Methods for making and using these plants, e.g., to produce food products having improved taste and to produce fermentable substrates for the production of ethanol and fermented beverages are also provided.
摘要翻译：本发明提供了多核苷酸，优选合成的多核苷酸，其编码为在植物中表达而优化的加工酶。多核苷酸编码嗜温，嗜热或超嗜热加工酶，其在合适的活化条件下活化以作用于所需的底物。还提供了“自加工”转基因植物和植物部分，例如表达这些酶中的一种或多种并且具有改变的组合物的植物和谷粒加工的植物部分。还提供了制备和使用这些植物的方法，例如生产具有改善味道的食品并生产用于生产乙醇和发酵饮料的可发酵底物。

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