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1. 发明申请

US20130109051A1 METHOD FOR DETECTING MICROORGANISMS, DEVICE FOR DETECTING MICROORGANISMS AND PROGRAM 有权
标题翻译：检测微生物的方法，用于检测微生物和程序的装置
公开(公告)号：US20130109051A1
公开(公告)日：2013-05-02
申请号：US13805631
申请日：2011-06-20
申请人： Shenglan Li , Takashi Nishida , Chizuka Kai , Kunimitsu Toyoshima
发明人： Shenglan Li , Takashi Nishida , Chizuka Kai , Kunimitsu Toyoshima
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , C12M41/46 , C12Q1/02 , G06K9/0014 , G06K9/6269 , G06T7/0012 , G06T2207/10024 , G06T2207/20081 , G06T2207/30024
摘要： A method for detecting microorganisms, which comprises: a training step for forming, by a classifier, feature vectors based on color data on individual points within a subject region of training in a culture medium, mapping the points in the culture medium, that are specified by the feature vectors, on a high-dimensional feature space, and linearly separating a set of the points ψ (x1), that are specified by the high-dimensional feature vectors thus obtained, to thereby color-classify the class (C1) of the culture medium; and a identifying step for forming, by a classifier, feature vectors based on color data on individual inspection points within a region in the culture medium using image data obtained by capturing an image of the culture medium under cultivation, mapping the inspection points (xj), that are specified by the feature vectors, on a high-dimensional feature space, and determining whether or not the mapped points ψ (xj), that are specified by the high-dimensional feature vectors thus obtained, belong to the class (C1) of the culture medium, thereby identifying a colony based on inspection points not belonging to the class (C1) of the culture medium.
摘要翻译：一种用于检测微生物的方法，其特征在于包括：训练步骤，用于通过分类器基于培养基中训练对象区域内的各个点上的颜色数据形成特征向量，对培养基中指定的点进行映射通过特征向量在高维特征空间上，并且由这样获得的高维特征向量线性分离由所获得的高维特征向量指定的一组点psi（x1），从而对类别（C1）进行颜色分类培养基; 以及识别步骤，通过使用通过捕获培养下的培养基的图像获得的图像数据，在分类器中形成基于在培养基中的区域内的各个检查点上的颜色数据的特征向量，将检查点（xj）由特征向量指定，在高维特征空间上，并且确定由如此获得的高维特征向量指定的映射点psi（xj）是否属于类（C1）的培养基，从而根据不属于培养基类（C1）的检查点来鉴定菌落。

2. 发明申请

US20100166271A1 Method for Counting Colonies 有权
标题翻译：计算殖民地的方法
公开(公告)号：US20100166271A1
公开(公告)日：2010-07-01
申请号：US12601544
申请日：2008-05-09
申请人： Takashi Nishida , Shenglan Li , Chizuka Kai , Kunimitsu Toyoshima
发明人： Takashi Nishida , Shenglan Li , Chizuka Kai , Kunimitsu Toyoshima
IPC分类号： G06K9/00
CPC分类号： C12Q1/06 , G06T7/11 , G06T7/136 , G06T2207/10056 , G06T2207/30024 , G06T2207/30242
摘要： Problem to be Solved There is provided a colony counting method capable of obtaining highly reliable and stable colony count values with easy and simple means by eliminating the variation in colony count due to image capturing conditions when the number of colonies is counted by performing data processing on an image obtained by image capturing means performing image capturing on a petri dish.Solution When the number of colonies is counted by performing image capturing and data processing on microbial colonies developed in a culture medium 1 in a petri dish 2 by a CCD camera 7 (image capturing means) provided in an extension direction of a rotational axis C1, which is set near a center of the culture medium surface if of the petri dish, the petri dish is rotated around the rotational axis by a specified angle obtained by dividing one rotation of the petri dish by a predetermined number, and image data of the entire culture medium surface of the petri dish is acquired for each rotation by a specified angle; the number of colonies at each specified angle is counted separately by performing data processing on image data of the entire culture medium surface of the petri dish at the specified angle; and the number of colonies in the petri dish is calculated by performing numerical processing on the number of colonies counted separately at each specified angle.
摘要翻译：要解决的问题提供了一种能够通过简单和简单的手段获得高度可靠和稳定的菌落计数值的菌落计数方法，通过在通过执行数据处理来计数菌落数量时，通过消除由于图像捕获条件引起的菌落计数的变化通过图像捕获装置获得的图像，其在培养皿上进行图像捕获。解决方案当通过在旋转轴线C1的延伸方向上设置的CCD照相机7（图像捕获装置）对培养皿2中的培养基1中培养的微生物菌落进行图像捕获和数据处理来计数菌落数时，其设置在培养基表面的中心附近，如果陪替氏培养皿中，培养皿围绕旋转轴旋转指定角度，通过将培养皿的旋转除以预定数量获得的特定角度，并且整个图像数据每个旋转获取培养皿的培养基表面一个特定的角度; 通过以规定的角度对培养皿的整个培养基表面的图像数据进行数据处理，分别计数每个指定角度的菌落数量; 并且通过对在每个特定角度分别计数的菌落数进行数值处理来计算陪替氏培养皿中的菌落数。

3. 发明授权

US08831313B2 Method for detecting microorganisms, device for detecting microorganisms and program 有权
标题翻译：微生物检测方法，微生物检测装置及程序
公开(公告)号：US08831313B2
公开(公告)日：2014-09-09
申请号：US13805631
申请日：2011-06-20
申请人： Shenglan Li , Takashi Nishida , Chizuka Kai , Kunimitsu Toyoshima
发明人： Shenglan Li , Takashi Nishida , Chizuka Kai , Kunimitsu Toyoshima
IPC分类号： G06K9/00 , C12M1/34 , C12Q1/04 , G06T7/00 , C12Q1/02 , G06K9/62
CPC分类号： C12Q1/04 , C12M41/46 , C12Q1/02 , G06K9/0014 , G06K9/6269 , G06T7/0012 , G06T2207/10024 , G06T2207/20081 , G06T2207/30024
摘要： A method for detecting microorganisms, which comprises: a training step for forming, by a classifier, feature vectors based on color data on individual points within a subject region of training in a culture medium, mapping the points in the culture medium, that are specified by the feature vectors, on a high-dimensional feature space, and linearly separating a set of the points ψ (x1), that are specified by the high-dimensional feature vectors thus obtained, to thereby color-classify the class (C1) of the culture medium; and a identifying step for forming, by a classifier, feature vectors based on color data on individual inspection points within a region in the culture medium using image data obtained by capturing an image of the culture medium under cultivation, mapping the inspection points (xj), that are specified by the feature vectors, on a high-dimensional feature space, and determining whether or not the mapped points ψ (xj), that are specified by the high-dimensional feature vectors thus obtained, belong to the class (C1) of the culture medium, thereby identifying a colony based on inspection points not belonging to the class (C1) of the culture medium.
摘要翻译：一种用于检测微生物的方法，其特征在于包括：训练步骤，用于通过分类器基于培养基中训练对象区域内的各个点上的颜色数据形成特征向量，对培养基中指定的点进行映射通过特征向量在高维特征空间上，并且由这样获得的高维特征向量线性分离由所获得的高维特征向量指定的点ψ（x1）的集合，从而对类别（C1）进行颜色分类培养基; 以及识别步骤，通过使用通过捕获培养下的培养基的图像获得的图像数据，在分类器中形成基于在培养基中的区域内的各检查点上的颜色数据的特征向量，将检查点（xj）由特征向量指定的高维特征空间，并且确定由如此获得的高维特征向量指定的映射点ψ（xj）是否属于类（C1）的培养基，从而根据不属于培养基类（C1）的检查点来鉴定菌落。

4. 发明授权

US08831326B2 Method for counting colonies 有权
标题翻译：计数菌落的方法
公开(公告)号：US08831326B2
公开(公告)日：2014-09-09
申请号：US12601544
申请日：2008-05-09
申请人： Takashi Nishida , Shenglan Li , Chizuka Kai , Kunimitsu Toyoshima
发明人： Takashi Nishida , Shenglan Li , Chizuka Kai , Kunimitsu Toyoshima
IPC分类号： G06K9/00 , C12Q1/06 , G06K9/62
CPC分类号： C12Q1/06 , G06T7/11 , G06T7/136 , G06T2207/10056 , G06T2207/30024 , G06T2207/30242
摘要： Colonies growing on a culture medium surface may be more accurately counted with reduced variation in the counts obtained by capturing a plurality of images of the entire culture medium surface, wherein successive images are captured following rotation of the culture medium surface relative to the image capture apparatus by n/360 degrees where n is the number of images to be captured; performing data processing on image data of the entire culture medium surface of the petri dish at each specified angle; and the number of colonies in the petri dish is calculated by performing numerical processing on the number of colonies counted separately at each specified angle.
摘要翻译：通过捕获整个培养基表面的多个图像而获得的计数的变化减小，可以更精确地计数在培养基表面上生长的菌落，其中在培养基表面相对于图像捕获装置旋转之后捕获连续的图像通过n / 360度，其中n是要捕获的图像的数量; 对每个特定角度的培养皿整个培养基表面的图像数据执行数据处理; 并且通过对在每个特定角度分别计数的菌落数进行数值处理来计算陪替氏培养皿中的菌落数。

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