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1. 发明授权

US08603806B2 Materials and methods for cell-based assays 有权
标题翻译：用于基于细胞的测定的材料和方法
公开(公告)号：US08603806B2
公开(公告)日：2013-12-10
申请号：US11556132
申请日：2006-11-02
申请人： Shang-Tian Yang , Xudong Zhang , Yuan Wen
发明人： Shang-Tian Yang , Xudong Zhang , Yuan Wen
IPC分类号： C12M1/22 , C12M3/00
CPC分类号： C12M23/12 , C12M25/14
摘要： The methods generally relate to three-dimensional culturing of cells with the steps of seeding a cell support structure in a device with at least one vessel for cell culture having a bottom, at least one wall and a cell support structure for three-dimensional cell culture. The cell support structure is inside the at least one vessel and the device is configured such that assays can be performed. Devices for performing cell culture and assays are also provided. It will be understood that the devices can have many different suitable configurations. In general, the devices are selected such that they can be used with suitable radiation based assays.
摘要翻译：所述方法通常涉及细胞的三维培养，其步骤是在具有至少一个细胞培养的容器的装置中接种细胞支持结构，所述容器具有底部，至少一个壁和用于三维细胞培养的细胞支持结构。细胞支持结构在至少一个容器内，并且该装置被配置为使得可以进行测定。还提供了用于进行细胞培养和测定的装置。应当理解，这些装置可以具有许多不同的合适构造。通常，选择这些装置使得它们可以与合适的基于辐射的测定一起使用。

2. 发明申请

US20070099294A1 MATERIALS AND METHODS FOR CELL-BASED ASSAYS 有权
标题翻译：基于细胞测定的材料与方法
公开(公告)号：US20070099294A1
公开(公告)日：2007-05-03
申请号：US11556132
申请日：2006-11-02
申请人： Shang-Tian Yang , Xudong Zhang , Yuan Wen
发明人： Shang-Tian Yang , Xudong Zhang , Yuan Wen
IPC分类号： C12M3/00
CPC分类号： C12M23/12 , C12M25/14
摘要： The methods generally relate to three-dimensional culturing of cells with the steps of seeding a cell support structure in a device with at least one vessel for cell culture having a bottom, at least one wall and a cell support structure for three-dimensional cell culture. The cell support structure is inside the at least one vessel and the device is configured such that assays can be performed. Devices for performing cell culture and assays are also provided. It will be understood that the devices can have many different suitable configurations. In general, the devices are selected such that they can be used with suitable radiation based assays.
摘要翻译：所述方法通常涉及细胞的三维培养，其步骤是在具有至少一个细胞培养用容器的装置中接种细胞支持结构，所述容器具有底部，至少一个壁和用于三维细胞培养的细胞支持结构。细胞支持结构在至少一个容器内，并且该装置被配置为使得可以进行测定。还提供了用于进行细胞培养和测定的装置。应当理解，这些装置可以具有许多不同的合适构造。通常，选择这些装置使得它们可以与合适的基于辐射的测定一起使用。

3. 发明授权

US07811796B2 Immobilization of enzyme on a fibrous matrix 有权
标题翻译：酶在纤维基质上的固定
公开(公告)号：US07811796B2
公开(公告)日：2010-10-12
申请号：US11633559
申请日：2006-12-04
申请人： Shang-Tian Yang , Nedim Albayrak
发明人： Shang-Tian Yang , Nedim Albayrak
IPC分类号： C12P19/04 , C12P19/02 , C12N11/18 , C12N11/02
CPC分类号： C12N11/06 , C12N11/08 , C12N11/12 , C12P19/02 , C12P19/18
摘要： A multilayer enzyme immobilization process is provided comprising adsorbing a polyethyleneimine (PEI) solution in a fibrous matrix, and adding an enzyme to the fibrous matrix, which comprises a plurality of fibrils. The process further comprises forming at least two layers of PEI-enzyme aggregates on the fibrils, and cross-linking the multilayer PEI-enzyme aggregates. The process can further comprise washing the fibrils containing the cross-linked PEI-enzyme aggregates with distilled water and acetic acid buffer subsequent to cross-linking. However, the PEI-containing matrix is not washed prior to the addition of enzyme. The enzyme can be β-galactosidase and the fibrous matrix can be cotton cloth. The multilayer immobilized enzyme can be employed in a biocatalyst reactor for production of galacto-oligosaccharides from lactose and the hydrolysis of lactose to glucose and galactose.
摘要翻译：提供了一种多层酶固定方法，包括将聚乙烯亚胺（PEI）溶液吸附在纤维基质中，并向包括多个原纤维的纤维基质中加入酶。该方法还包括在原纤维上形成至少两层PEI-酶聚集体，并交联多层PEI-酶聚集体。该方法可以进一步包括在交联后用蒸馏水和乙酸缓冲液洗涤含有交联的PEI-酶聚集体的原纤维。然而，含有PEI的基质在加入酶之前不被洗涤。酶可以是β-半乳糖苷酶，纤维基质可以是棉布。多层固定化酶可用于生物催化剂反应器中，用于从乳糖制备低聚半乳糖，并将乳糖水解成葡萄糖和半乳糖。

4. 发明申请

US20100124773A1 METHODS AND PROCESSES FOR PRODUCING ESTERS 有权
标题翻译：生产蛋白质的方法和方法
公开(公告)号：US20100124773A1
公开(公告)日：2010-05-20
申请号：US12621982
申请日：2009-11-19
申请人： Shang-Tian Yang
发明人： Shang-Tian Yang
IPC分类号： C12P7/62
CPC分类号： C12P7/40 , C12N9/1217 , C12N9/20 , C12N11/00 , C12P7/62
摘要： Methods are provided for producing esters. The methods comprise converting a fermentable carbon source to organic acids by fermentation with organic acid producing microorganisms, followed by catalytic esterification. The methods comprise integrated fermentation, extraction, and esterification reactions wherein the organic acids produced during fermentation are extracted into an extraction solvent and then directly reacted with an alcohol in the presence of a catalyst to form organic esters. Methods of producing esters are also provided wherein the organic acids produced during fermentation and extracted into the extraction solvent are stripped from the extraction solvent prior to being reacted with an alcohol in the presence of a catalyst to form organic esters.
摘要翻译：提供用于生产酯的方法。所述方法包括通过用产生有机酸的微生物发酵将可发酵碳源转化为有机酸，然后进行催化酯化。所述方法包括一体化发酵，提取和酯化反应，其中在发酵过程中产生的有机酸被萃取到萃取溶剂中，然后在催化剂存在下直接与醇反应形成有机酯。还提供了生产酯的方法，其中在催化剂存在下，在与醇反应形成有机酯之前，将在发酵过程中产生并提取到萃取溶剂中的有机酸从萃取溶剂中汽提出来。

5. 发明授权

US09284580B2 Metabolic engineering of clostridium tyrobutyricum for butanol production 有权
标题翻译：丁酸梭菌的代谢工程
公开(公告)号：US09284580B2
公开(公告)日：2016-03-15
申请号：US13877332
申请日：2011-09-30
申请人： Shang-Tian Yang , Mingrui Yu
发明人： Shang-Tian Yang , Mingrui Yu
IPC分类号： A61K38/00 , C12P7/16 , C12N9/04 , C12N9/02 , C12N15/74
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N15/74 , Y02E50/10
摘要： This invention relates to compositions, systems, and methods for producing biofuels, such as butanol, and related compounds. More specifically, provided are methods of making recombinant microorganizms having non-naturally occurring metabolic pathways for the production of biofuels, and methods of producing biofuels using such organizms. Also provided are metabolically engineered microorganizms capable of producing butanol from a substrate.
摘要翻译：本发明涉及用于生产生物燃料的组合物，体系和方法，例如丁醇和相关化合物。更具体地，提供了制备具有用于生产生物燃料的非天然存在的代谢途径的重组微生物的方法，以及使用这种组织生产生物燃料的方法。还提供能够从底物产生丁醇的代谢工程微生物。

6. 发明申请

US20090123336A1 MICROFLUIDIC CHIPS FOR RAPID MULTIPLEX ELISA 有权
标题翻译：用于快速多重ELISA的微流感病毒
公开(公告)号：US20090123336A1
公开(公告)日：2009-05-14
申请号：US11937001
申请日：2007-11-08
申请人： Shang-Tian Yang , Yunling Bai , Wei-Cho Huang
发明人： Shang-Tian Yang , Yunling Bai , Wei-Cho Huang
IPC分类号： B01J19/00 , G01N33/00
CPC分类号： B01L3/5027 , B01L2200/10 , B01L2200/16 , B01L2300/0627 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0829 , B01L2300/087 , B01L2300/088 , B01L2400/0406 , G01N35/028 , G01N2035/00158 , G01N2035/00514
摘要： A microfluidic chip comprises a substrate and a channel or multiple channels in the substrate. Each channel includes a tortuous path section or multiple tortuous path sections. A receptor for the detection of an analyte can be immobilized in a tortuous path section, for example by adsorption. Different receptors can be immobilized in different tortuous path sections of each channel or in different channels for simultaneous detection of multiple analytes. The chip is especially useful for running immunoassays, particularly ELISA.
摘要翻译：微流体芯片包括衬底和衬底中的沟道或多个沟道。每个通道包括曲折路径部分或多个曲折路径部分。用于检测分析物的受体可以例如通过吸附固定在曲折路径部分中。不同的受体可以固定在每个通道的不同曲折路径部分或不同的通道中，以同时检测多种分析物。该芯片对于运行免疫测定法尤其是ELISA尤其有用。

7. 发明申请

US20080248540A1 METHODS OF PRODUCING BUTANOL 审中-公开
标题翻译：生产丁醇的方法
公开(公告)号：US20080248540A1
公开(公告)日：2008-10-09
申请号：US12062091
申请日：2008-04-03
申请人： Shang-Tian Yang
发明人： Shang-Tian Yang
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , Y02E50/10
摘要： A method of producing butanol from carbohydrates is provided. A feedstock comprising a carbohydrate source is fermented in the presence of bacteria to produce butyric acid and hydrogen. The butyric acid is then hydrogenated in the presence of a catalyst to produce butanol.
摘要翻译：提供了从碳水化合物生产丁醇的方法。包含碳水化合物源的原料在细菌存在下发酵以产生丁酸和氢。然后在催化剂存在下氢化丁酸，生成丁醇。

8. 发明申请

US20130323809A1 METABOLIC ENGINEERING OF CLOSTRIDIUM TYROBUTYRICUM FOR BUTANOL PRODUCTION 有权
标题翻译：代谢产物的代谢工程
公开(公告)号：US20130323809A1
公开(公告)日：2013-12-05
申请号：US13877332
申请日：2011-09-30
申请人： Shang-Tian Yang , Mingrui Yu
发明人： Shang-Tian Yang , Mingrui Yu
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/0008 , C12N15/74 , Y02E50/10
摘要： This invention relates to compositions, systems, and methods for producing biofuels, such as butanol, and related compounds. More specifically, provided are methods of making recombinant microorganisms having non-naturally occurring metabolic pathways for the production of biofuels, and methods of producing biofuels using such organisms. Also provided are metabolically engineered microorganisms capable of producing butanol from a substrate.
摘要翻译：本发明涉及用于生产生物燃料的组合物，系统和方法，例如丁醇和相关化合物。更具体地，提供了制备具有用于生产生物燃料的非天然存在的代谢途径的重组微生物的方法，以及使用这种生物体生产生物燃料的方法。还提供了能够从底物生产丁醇的代谢工程微生物。

9. 发明申请

US20070178586A1 METHODS AND APPARATUSES FOR GROWING CELLS 审中-公开
标题翻译：培养细胞的方法和装置
公开(公告)号：US20070178586A1
公开(公告)日：2007-08-02
申请号：US11558396
申请日：2006-11-09
申请人： Shang-Tian Yang , Anil Ouyang
发明人： Shang-Tian Yang , Anil Ouyang
IPC分类号： C12N5/06 , C12M3/00
CPC分类号： C12M25/14 , C12M23/58 , C12M29/10
摘要： Methods of culturing stem cells including growing fibroblast cells on a three-dimensional scaffold, perfusing the fibroblast cells with a cell culture medium to form fibroblast cell-conditioned cell culture medium, and growing the stem cells on a three-dimensional scaffold perfused with the fibroblast cell-conditioned cell culture medium are presented. Multi-stage bioreactors for growing stem cells, comprising a first fibrous bed bioreactor in fluid communication with a second fibrous bed bioreactor are also presented.
摘要翻译：培养包括在三维支架上培养成纤维细胞的干细胞的方法，用细胞培养基灌注成纤维细胞以形成成纤维细胞细胞培养细胞，并将干细胞生长在用成纤维细胞灌注的三维支架上呈现细胞培养细胞培养基。还提出了用于生长干细胞的多级生物反应器，包括与第二纤维床生物反应器流体连通的第一纤维床生物反应器。

10. 发明申请

US20070111289A1 Immobilization of enzyme on a fibrous matrix 有权
公开(公告)号：US20070111289A1
公开(公告)日：2007-05-17
申请号：US11633559
申请日：2006-12-04
申请人： Shang-Tian Yang , Nedim Albayrak
发明人： Shang-Tian Yang , Nedim Albayrak
IPC分类号： C12P19/04 , C12N11/04
CPC分类号： C12N11/06 , C12N11/08 , C12N11/12 , C12P19/02 , C12P19/18
摘要： A multilayer enzyme immobilization process is provided comprising adsorbing a polyethyleneimine (PEI) solution in a fibrous matrix, and adding an enzyme to the fibrous matrix, which comprises a plurality of fibrils. The process further comprises forming at least two layers of PEI-enzyme aggregates on the fibrils, and cross-linking the multilayer PEI-enzyme aggregates. The process can further comprise washing the fibrils containing the cross-linked PEI-enzyme aggregates with distilled water and acetic acid buffer subsequent to cross-linking. However, the PEI-containing matrix is not washed prior to the addition of enzyme. The enzyme can be β-galactosidase and the fibrous matrix can be cotton cloth. The multilayer immobilized enzyme can be employed in a biocatalyst reactor for production of galacto-oligosaccharides from lactose and the hydrolysis of lactose to glucose and galactose.

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