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1. 发明授权

US4613567A Immunoassay method for measuring immunological antigen in fluid sample 失效
标题翻译：用于测量流体样品中免疫学抗原的免疫测定方法
公开(公告)号：US4613567A
公开(公告)日：1986-09-23
申请号：US520072
申请日：1983-08-03
申请人： Seikichi Yasoshima , Mikio Koyama , Kenichiro Okaniwa
发明人： Seikichi Yasoshima , Mikio Koyama , Kenichiro Okaniwa
IPC分类号： G01N33/543 , C12Q1/00 , G01N21/64 , G01N33/558
CPC分类号： G01N33/54386 , Y10S435/805 , Y10S435/97 , Y10S436/807
摘要： There is disclosed an immunoassay comprising carrying out an immunological reaction by contacting a fluid sample with an element for immunoassay, having at least three layers of a first detecting layer, a blocking layer and a second detecting layer which are successively laminated, the element containing a substance capable of binding specifically to an immunologically active substance in a fluid sample only in either one of the first and the second detecting layers, and then measuring the detected quantities corresponding to the immunological reaction quantities from both of the first and the second detecting layers.The immunoassay by the use of the immunological analytical element according to this invention improves the precision immunoassay by measuring both of Bound and Free.
摘要翻译：公开了一种免疫测定，其包括通过使流体样品与用于免疫测定的元件接触而进行免疫反应，所述元件具有至少三层依次层压的第一检测层，阻挡层和第二检测层，所述元件含有能够仅在第一和第二检测层中的任一个中在流体样品中特异性结合免疫活性物质的物质，然后测量对应于来自第一和第二检测层的免疫反应量的检测量。通过使用根据本发明的免疫分析元件的免疫测定通过测量结合和自由来提高精确的免疫测定。

2. 发明授权

US4594225A Elements for analysis of calcium 失效
标题翻译：钙分析要素
公开(公告)号：US4594225A
公开(公告)日：1986-06-10
申请号：US775709
申请日：1985-09-13
申请人： Kazumi Arai , Mikio Koyama , Kenichiro Okaniwa
发明人： Kazumi Arai , Mikio Koyama , Kenichiro Okaniwa
IPC分类号： G01N33/84 , G01N21/78 , G01N31/00 , G01N31/22 , G01N33/52
CPC分类号： G01N31/22
摘要： An improved multilayer analytical element for the determination of calcium comprising a liquid impermeable, optically transparent support; at least one reagent layer positioned with a first side on said support and containing at least one reagent capable of reacting with a calcium component in a fluid sample; and at least one porous developing layer on the second side of said reagent layer, said porous developing layer being permeable to calcium from said fluid sample thereby transporting said calcium to said reagent layer. The improvement comprises at least one of said reagent layer and developing layer containing a basic substance of the formula M.sub.v H.sub.w PO.sub.4 .multidot.ZH.sub.2 O wherein M is lithium, sodium or potassium; v is an integer of 2 or 3 and w is an integer of 0 or 1 provided that v plus w equals 3; and Z is 1/2 when M is lithium, 0, 2 or 12 when M is sodium and 0 or 8 when M is potassium; and said reagent layer contains (i) 3,3'-bis[N,N-di(carboxymethyl)aminomethyl]-o-cresolphthalein and (ii) 8-hydroxyquinoline or a derivative thereof.
摘要翻译：一种用于测定钙的改进的多层分析元件，其包含不透液体，光学透明的载体; 至少一个试剂层，其具有位于所述载体上的第一侧，并含有至少一种能够与流体样品中的钙组分反应的试剂; 以及在所述试剂层的第二侧上的至少一个多孔显影层，所述多孔显影层可从所述流体样品渗透钙，从而将所述钙输送到所述试剂层。该改进包括至少一个所述试剂层和显影层，其含有式MvHwPO4xZH2O的碱性物质，其中M是锂，钠或钾; v是2或3的整数，w是0或1的整数，条件是v plus w等于3; 当M为锂时，Z为1/2，当M为钠时Z为0或2，当M为钾时为0或8; 并且所述试剂层含有（i）3,3'-双[N，N-二（羧甲基）氨基甲基] - 邻 - 甲酚酞和（ii）8-羟基喹啉或其衍生物。

3. 发明授权

US4567136A Analytical element 失效
标题翻译：分析要素
公开(公告)号：US4567136A
公开(公告)日：1986-01-28
申请号：US618311
申请日：1984-06-07
申请人： Kenichiro Okaniwa , Mikio Koyama , Shozo Kikugawa
发明人： Kenichiro Okaniwa , Mikio Koyama , Shozo Kikugawa
IPC分类号： C12Q1/28 , G01N31/00 , G01N31/22 , G01N33/50 , G01N33/52 , G01N33/66 , G01N33/92 , C12Q1/00 , G01N21/78
CPC分类号： G01N33/526 , Y10S435/805
摘要： There is disclosed an analytical element which comprises a support and a reagent layer comprising a peroxidative substance, a diffusion-resistant phenol compound, and an aromatic primary amine compound or its salt which brings about a coupling reaction with said diffusion-resistant phenol compound when oxidized, to thereby produce a dyestuff.
摘要翻译：公开了一种分析元件，其包含载体和试剂层，所述载体和试剂层包含过氧化物质，扩散阻抗性酚化合物和芳族伯胺化合物或其盐，当被氧化时与所述扩散性酚化合物发生偶联反应，从而产生染料。

4. 发明授权

US4557901A Analytical element 失效
标题翻译：分析要素
公开(公告)号：US4557901A
公开(公告)日：1985-12-10
申请号：US437691
申请日：1982-10-29
申请人： Mikio Koyama , Kenichiro Okaniwa , Masakuni Saruhashi , Yuko Ohmachi
发明人： Mikio Koyama , Kenichiro Okaniwa , Masakuni Saruhashi , Yuko Ohmachi
IPC分类号： C12Q1/00 , G01N31/22 , G01N33/52 , G01N21/78
CPC分类号： G01N33/525 , Y10S435/805
摘要： An analytical element for use in analysis of a component in an aqueous fluid, comprising(a) a light-transmissive support;(b) a reagent layer; and(c) a porous spreading layer,being characterized in that the reagent layer contains a substantially water-dispersible copolymer obtained by copolymerization of at least one copolymerizable monomer, which is water soluble and has a nonionizable group in the side chain, with at least one copolymerizable hydrophobic monomer.The analytical element of this invention is good with less formation of fogging, higher in color forming density and indicating higher sensitivity.
摘要翻译：一种用于分析含水流体中的组分的分析元件，包括（a）透光支撑体; （b）试剂层; 和（c）多孔扩散层，其特征在于，试剂层含有通过至少一种可水解且在侧链中具有不可分解基团的可共聚单体与至少一种共聚合得到的基本上水分散性共聚物一种可共聚的疏水性单体。本发明的分析元件具有较好的雾化形成，较高的成色密度和较高的灵敏度。

5. 发明授权

US4551307A Analytical element 失效
标题翻译：分析要素
公开(公告)号：US4551307A
公开(公告)日：1985-11-05
申请号：US381794
申请日：1982-05-25
申请人： Mikio Koyama , Shozo Kikugawa , Kenichiro Okaniwa
发明人： Mikio Koyama , Shozo Kikugawa , Kenichiro Okaniwa
IPC分类号： G01N31/22 , G01N33/52 , G01N33/92
CPC分类号： G01N33/525 , Y10S435/805
摘要： There is disclosed an analytical element comprising a light-transmissive and liquid-impermeable support and at least one developing layer of a fibrous structure containing at least one kind of interactive composition reactive with a component in a liquid sample, characterized in that the fiber constituting the developing layer of a fibrous structure and the interactive composition are bonded to each other by a compound having a reactive group. A quantitative analysis of a specific component in a liquid sample can effectively be carried out by using the analytical element according to this invention.
摘要翻译：公开了一种分析元件，其包括透光和液体不可渗透的支撑体和至少一个纤维结构的显影层，所述显影层含有与液体样品中的组分反应的至少一种相互作用组合物，其特征在于，构成纤维结构的显影层和相互作用组合物通过具有反应性基团的化合物彼此键合。通过使用根据本发明的分析元件可以有效地进行液体样品中特定成分的定量分析。

6. 发明授权

US4914020A Multi-layer analytical element with preserved enzyme containing spreading layer 失效
标题翻译：多层分析元素与保存酶含有扩散层
公开(公告)号：US4914020A
公开(公告)日：1990-04-03
申请号：US824450
申请日：1986-01-31
申请人： Kazumi Arai , Mikio Koyama , Morio Kobayashi , Kenichiro Okaniwa , Takasi Momose , Kunihiro Furukawa , Souichi Zanma
发明人： Kazumi Arai , Mikio Koyama , Morio Kobayashi , Kenichiro Okaniwa , Takasi Momose , Kunihiro Furukawa , Souichi Zanma
IPC分类号： C12Q1/00 , C12Q1/26 , C12Q1/28 , C12Q1/34 , G01N31/22 , G01N33/52
CPC分类号： G01N33/525
摘要： An analytical element to measure a specific component in a fluid samples comprising support, a layer containing a reagent provided the support and a spreading layer provided on above the reagent containing layer. In the spreading layer, an emzyme necessary for the reaction to produce a product capable of being detected with said reagent from the specific component is contained. The enzyme is protected from deterioration of activity during a manufacturing and preservation of the analytical element by means of the enzyme is included in the spread layer as a dispersion mixture with a protein and/or polypeptide. An accurate analytical result is stably obtained by the analytical element.
摘要翻译：一种用于测量包含载体的流体样品中的特定组分的分析元件，含有提供所述载体的试剂的层和设置在所述试剂含有层上方的扩散层。在扩散层中，含有产生能够从特定成分用所述试剂检测的产物的反应所需的酶。在通过酶的制造和保存分析元件期间保护酶免于活性的恶化，作为与蛋白质和/或多肽的分散混合物在扩散层中包括。通过分析元件稳定地获得准确的分析结果。

7. 发明授权

US4576793A Analytical element 失效
标题翻译：分析要素
公开(公告)号：US4576793A
公开(公告)日：1986-03-18
申请号：US527681
申请日：1983-08-29
申请人： Mikio Koyama , Kenichiro Okaniwa , Masakuni Saruhashi
发明人： Mikio Koyama , Kenichiro Okaniwa , Masakuni Saruhashi
IPC分类号： G01N31/22 , G01N33/52 , G01N21/78
CPC分类号： G01N31/22 , G01N33/525 , Y10S435/805 , Y10T436/172307
摘要： There is disclosed an analytical element comprising a support; a reagent layer which is positioned on one side of the support and contains a reagent reactive with a component in a fluid sample; and a porous spreading layer which is positioned on the reagent layer on the opposite side to that of the support for permitting the component in the fluid sample to permeate into the reagent layer; wherein at least one layer contains 5% by weight or more of a polymer formed by, (i) a copolymerizable ethylenically unsaturated acid with (ii) a copolymerizable ethylenically unsaturated monomer.The analytical element according to the present invention can determine a component in a fluid sample quantitatively.
摘要翻译：公开了一种包括支撑体的分析元件; 试剂层，其位于所述载体的一侧，并且包含与流体样品中的组分反应的试剂; 以及多孔扩散层，其位于与支撑体相反侧的试剂层上，以允许流体样品中的组分渗透到试剂层中; 其中至少一层包含5重量％以上的由（i）可共聚的烯属不饱和酸与（ii）可共聚的烯属不饱和单体形成的聚合物。根据本发明的分析元件可以定量地确定流体样品中的组分。

8. 发明授权

US5087421A Multilayered assay element 失效
标题翻译：多层测定元件
公开(公告)号：US5087421A
公开(公告)日：1992-02-11
申请号：US353942
申请日：1989-05-19
申请人： Mikio Koyama , Satoshi Kawakatsu , Tomoji Akashi , Kenichiro Okaniwa
发明人： Mikio Koyama , Satoshi Kawakatsu , Tomoji Akashi , Kenichiro Okaniwa
IPC分类号： G01N31/22 , G01N21/78 , G01N33/52 , G01N33/543 , G01N33/72
CPC分类号： G01N33/728 , G01N33/523
摘要： Disclosed is a multilayered assay element in which the influence by the bilirubin contained in the sample fluid is substantially eliminated. The assay element of the present invention contains a bilirubin-trapping agent of the formula(A).sub.x (B).sub.y (C).sub.z [I](wherein A represents a monomer unit having a copolymerizable unsaturated double bond and a quaternary ammonium salt, B represents a monomer unit having a copolymerizable unsaturated double bond, C represents a monomer unit having at least two copolymerizable unsaturated double bonds and x, y and z represents the number mol % of 100-10, 90-0 and 10-0, respectively) which bilirubin-trapping agent is incorporated in a region which is upper than the reagent layer.
摘要翻译：公开了一种多层测定元件，其中基本上消除了样品流体中所含的胆红素的影响。本发明的测定元件含有式（A）x（B）y（C）z [I]（其中A表示具有可共聚不饱和双键的单体单元和季铵盐）的胆红素捕获剂， B表示具有可共聚的不饱和双键的单体单元，C表示具有至少两个可共聚的不饱和双键的单体单元，x，y和z分别表示100-10,90-0和10-0的数摩尔％），其中胆红素捕获剂加入到比试剂层高的区域中。

9. 发明授权

US4567024A Analytical element 失效
标题翻译：分析要素
公开(公告)号：US4567024A
公开(公告)日：1986-01-28
申请号：US456837
申请日：1983-01-10
申请人： Mikio Koyama , Kenichiro Okaniwa
发明人： Mikio Koyama , Kenichiro Okaniwa
IPC分类号： G01N31/22 , G01N33/52 , G01N21/78
CPC分类号： G01N33/525 , Y10S435/805
摘要： An analytical element comprising a light-transmissive and liquid-impervious support having thereon, in order from the support side, at least one reagent layer containing at least one kind of reagent reactive with a component in a fluid sample, a shielding layer and at least one porous spreading layer, said shielding layer comprising at least a white pigment and a substantially water-dispersible reactive polymer which comprises a copolymer having (i) monomeric units which have a reactive group and (ii) at least one kind of other hydrophobic monomer.
摘要翻译：一种分析元件，包括透光性和液体不可渗透性的载体，其从支撑侧依次具有至少一个试剂层，其包含至少一种与流体样品中的组分反应的试剂，屏蔽层和至少一个多孔扩散层，所述屏蔽层至少包含白色颜料和基本上水分散的反应性聚合物，其包含具有（i）具有反应性基团的单体单元和（ii）至少一种其它疏水性单体的共聚物。

10. 发明授权

US4430436A Analytical element and method of use 失效
标题翻译：分析要素和使用方法
公开(公告)号：US4430436A
公开(公告)日：1984-02-07
申请号：US329034
申请日：1981-12-09
申请人： Mikio Koyama , Shozo Kikugawa , Kenichiro Okaniwa , Kiyoshi Tamaki
发明人： Mikio Koyama , Shozo Kikugawa , Kenichiro Okaniwa , Kiyoshi Tamaki
IPC分类号： G01N21/78 , G01N31/22 , G01N33/52 , G01N33/54
CPC分类号： G01N33/525 , G01N31/22 , Y10S436/81 , Y10S525/902 , Y10T428/254 , Y10T428/2998 , Y10T428/31536
摘要： An analytical element for analysis of a liquid having an interconnected void structure zone positioned on one side of a liquid-impermeable, light-transmissive support, being characterized in that said interconnected void structure zone comprises a bound particulate structure which is a three-dimensional lattice, being non-swellable in the liquid and having interconnected voids with a void volume of 25 to 85% among the particles to provide transport of said liquid, the material for said bound particulate structure being non-swellable in and impermeable to said liquid and said bound particulate structure being constituted of heat-stable, organic polymer particle units having reactive groups with particle sizes of 1 to 350 microns which are chemically bonded directly to each other through said reactive groups in the areas of contact. A method of analysis utilizing the analytical element in immunoassay is also disclosed.
摘要翻译：一种用于分析液体的分析元件，其具有位于不透液体的透光支架的一侧上的互连的空隙结构区域，其特征在于，所述互连的空隙结构区域包括结合的颗粒结构，其是三维网格在液体中不溶胀并且在颗粒之间具有25至85％的空隙体积的互连空隙以提供所述液体的输送，所述结合的颗粒结构的材料在所述液体中是不溶胀的且不可渗透的，并且所述结合的颗粒结构由具有1至350微米的反应性基团的热稳定的有机聚合物颗粒单元构成，其通过接触区域中的所述反应性基团彼此直接化学键合。还公开了利用免疫测定中的分析元件的分析方法。

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