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1. 发明授权

US07175984B2 Identification and applications of porcine reproductive and respiratory syndrome virus host susceptibility factor(s) for improved swine breeding and development of a non-simian recombinant cell line for propagation of the virus and a target for a novel class of antiviral compounds 失效
标题翻译：识别和应用猪繁殖与呼吸综合征病毒宿主易感性因子，用于改善猪繁殖和开发用于病毒繁殖的非猿类重组细胞系和新型抗病毒化合物的靶标
公开(公告)号：US07175984B2
公开(公告)日：2007-02-13
申请号：US10058597
申请日：2002-01-28
申请人： Sanjay Kapil , Kumar Shanmukhappa
发明人： Sanjay Kapil , Kumar Shanmukhappa
IPC分类号： C12Q1/68 , C12N1/20 , C12N5/00 , C07H19/00
CPC分类号： C12N7/00 , A01K2217/05 , A01K2227/108 , A01K2267/02 , A61K39/00 , C07K14/70596 , C12N2770/38011 , C12N2770/38022 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , G01N33/56983 , G01N33/6872 , G01N2333/70596
摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) causes serious economic losses in swine. The present invention determined that CD 151 is a susceptibility factor for PRRSV infection by transfecting a cell line which is not susceptible to PRRSV infection (BHK-21) with CD 151, which rendered the cell line susceptible. Because CD 151 can be accessed in cellular material including blood platelets and germplasm, the present invention provides a non-invasive method of screening different swine for susceptibility to PRRSV, thereby improving breeding plans. In the case of a valuable animal, results from such screening can indicate any offspring's susceptibility to PPRSV. Additionally, the viral RNA-CD 151 interaction possesses high affinity and can be used as a tool to detect anti-viral compounds which can be further improved by using combinatorial chemistry. Accordingly, anti-viral compounds that can block the viral RNA-CD 151 interaction can be developed. Advantageously, transfection of CD 151 into non-simian cell lines can confer susceptibility to PRRSV and these recombinant cell lines can be used for preparation of biologics that will avoid simian cell lines which could be a source of primate viruses in xenotransplanted organs from pigs. Finally, the present invention describes the basic mechanism by which the virus RNA enters a target cell. This novel class of proteins is termed viral RNA entry proteins and a novel class of compounds named anti-RNA Entry Proteins can be used to block the entry of viral RNA, thereby preventing viral infections.
摘要翻译：猪繁殖与呼吸综合征病毒（PRRSV）导致猪严重经济损失。本发明通过用CD151转染对PRRSV感染（BHK-21）不敏感的细胞系（其使细胞系易感）确定CD151是PRRSV感染的易感因子。由于CD 151可以在包括血小板和种质的细胞材料中进行，所以本发明提供了对PRRSV易感性的不同猪筛选的非侵入性方法，从而改善育种计划。在有价值的动物的情况下，这种筛选的结果可以指示任何后代对PPRSV的易感性。此外，病毒RNA-CD 151相互作用具有高亲和力，可用作检测抗病毒化合物的工具，可通过组合化学进一步改善。因此，可以开发可以阻断病毒RNA-CD 151相互作用的抗病毒化合物。有利地，将CD 151转染到非猿猴细胞系中可以赋予PRRSV的易感性，并且这些重组细胞系可用于制备生物制剂，其将避免可能是来自猪的异种移植器官中的灵长类动物病毒源的猿猴细胞系。最后，本发明描述了病毒RNA进入靶细胞的基本机制。这种新型的蛋白质类型被称为病毒RNA进入蛋白质，一类名为抗RNA进入蛋白质的化合物可用于阻断病毒RNA的进入，从而防止病毒感染。

2. 发明授权

US06740490B2 Identification and applications of porcine reproductive and respiratory syndrome virus host susceptibility factor(s) for improved swine breeding 失效
标题翻译：鉴定和应用猪繁殖与呼吸综合征病毒宿主易感因子，改善猪繁殖
公开(公告)号：US06740490B2
公开(公告)日：2004-05-25
申请号：US09772044
申请日：2001-01-29
申请人： Sanjay Kapil , Kumar Shanmukhappa
发明人： Sanjay Kapil , Kumar Shanmukhappa
IPC分类号： C12Q168
CPC分类号： C12N7/00 , A01K2217/05 , A01K2227/108 , A01K2267/02 , A61K39/00 , C07K14/70596 , C12N2770/38011 , C12N2770/38022 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , G01N33/56983 , G01N33/6872 , G01N2333/70596
摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) causes serious economic losses in swine. The present invention determined that CD 151 is a susceptibility factor for PRRSV infection by transfecting a cell line which is not susceptible to PRRSV infection (BHK-21) with CD 151, which rendered the cell line susceptible. Because CD 151 can be accessed in cellular material including blood platelets and germplasm, the present invention provides a non-invasive method of screening different swine for susceptibility to PRRSV, thereby improving breeding plans. In the case of a valuable animal, results from such screening can indicate any offspring's susceptibility to PPRSV. Additionally, the viral RNA-CD 151 interaction possesses high affinity and can be used as a tool to detect anti-viral compounds which can be further improved by using combinatorial chemistry. Accordingly, anti-viral compounds that can block the viral RNA-CD 151 interaction can be developed. Advantageously, transfection of CD 151 into non-simian cell lines can confer susceptibility to PRRSV and these recombinant cell lines can be used for preparation of biologics that will avoid simian cell lines which could be a source of primate viruses in xenotransplanted organs from pigs. Finally, the present invention describes the basic mechanism by which the virus RNA enters a target cell. This novel class of proteins is termed viral RNA entry proteins and a novel class of compounds named anti-RNA Entry Proteins can be used to block the entry of viral RNA, thereby preventing viral infections.
摘要翻译：猪繁殖与呼吸综合征病毒（PRRSV）导致猪严重经济损失。本发明通过用CD151转染对PRRSV感染（BHK-21）不敏感的细胞系（其使细胞系易感）确定CD151是PRRSV感染的易感因子。由于CD 151可以在包括血小板和种质的细胞材料中进行，所以本发明提供了对PRRSV易感性的不同猪筛选的非侵入性方法，从而改善育种计划。在有价值的动物的情况下，这种筛选的结果可以指示任何后代对PPRSV的易感性。此外，病毒RNA-CD 151相互作用具有高亲和力，可用作检测抗病毒化合物的工具，可通过组合化学进一步改善。因此，可以开发可以阻断病毒RNA-CD 151相互作用的抗病毒化合物。有利地，将CD 151转染到非猿猴细胞系中可以赋予PRRSV的易感性，并且这些重组细胞系可用于制备生物制剂，其将避免可能是来自猪的异种移植器官中的灵长类动物病毒源的猿猴细胞系。最后，本发明描述了病毒RNA进入靶细胞的基本机制。这种新型的蛋白质类型被称为病毒RNA进入蛋白质，一类名为抗RNA进入蛋白质的化合物可用于阻断病毒RNA的进入，从而防止病毒感染。

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