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1. 发明授权

US08003850B2 Method for producing target proteins using amino acids and pyruvic acids in culture of plant cells 失效
标题翻译：在植物细胞培养中使用氨基酸和丙酮酸生产靶蛋白的方法
公开(公告)号：US08003850B2
公开(公告)日：2011-08-23
申请号：US12160049
申请日：2007-09-19
申请人： Sang-Lin Kim , Hyun-Kwang Tan , Sang-Min Lim , Wuk-Sang Ryu , Hahn-Sun Jung , Song-Jae Lee , Cheon-Ik Park , Seung-Hoon Kang , Dong-Il Kim
发明人： Sang-Lin Kim , Hyun-Kwang Tan , Sang-Min Lim , Wuk-Sang Ryu , Hahn-Sun Jung , Song-Jae Lee , Cheon-Ik Park , Seung-Hoon Kang , Dong-Il Kim
IPC分类号： C12N15/63 , C12N15/00 , C12N15/82 , A01H5/00
CPC分类号： C12P21/00 , A01H4/00 , C12N15/8237
摘要： Provided is a method for producing a target protein via cultivation of transgenic plant cells comprising a promoter capable of expressing the protein under sugar-free conditions in the response to the depletion of sugar and a gene encoding the target protein, without exchange of a cell growth medium with a sugar-depleted medium comprising the addition of an amino acid mixture to the sugar-rich medium used to grow the plant cells.
摘要翻译：提供了通过培养转基因植物细胞来生产靶蛋白的方法，所述转基因植物细胞包含能够在无糖条件下表达蛋白质的启动子，以响应于糖的消耗和编码靶蛋白的基因，而不交换细胞生长培养基中加入含糖的培养基，包括向用于生长植物细胞的富糖培养基中加入氨基酸混合物。

2. 发明申请

US20100189717A1 Plant Recombinant Human CTLA4IG and a Method for Producing the Same 审中-公开
标题翻译：植物重组人CTLA4IG及其生产方法
公开(公告)号：US20100189717A1
公开(公告)日：2010-07-29
申请号：US12668766
申请日：2008-07-09
申请人： Sang-Lin Kim , Hyun-Kwang Tan , Sang-Min Lim , Wuk-Sang Ryu , Hahn-Sun Jung , Song-Jae Lee , Cheon-Ik Park , Seung-Hoon Kang , Dong Il Kim
发明人： Sang-Lin Kim , Hyun-Kwang Tan , Sang-Min Lim , Wuk-Sang Ryu , Hahn-Sun Jung , Song-Jae Lee , Cheon-Ik Park , Seung-Hoon Kang , Dong Il Kim
IPC分类号： A61K39/395 , C12N15/74 , C12N5/10 , C12P21/00 , C07K16/18 , A61P37/04
CPC分类号： C07K14/70521 , C07K2319/30 , C12N9/1051 , C12N15/8257 , C12P21/005
摘要： The present invention provides a recombinant vector pBI-3D-hGalT or pBI-35S-hGalT containing a human β1,4-galactosyltransferase gene; a cell line transformed with a recombinant vector pMYN414 containing a cytotoxic T-lymphocyte anti-gen A-immunoglobulin (CTLA4Ig) fusion protein gene and the recombinant vector pBI-3D-hGalT or pBI-355-hGalT; and a method for producing a plant-derived recombinant human CTLA4Ig (CTLA4Igp) fusion protein with a human glycan structure using the same. The plant cell-derived recombinant human CTLA4Ig fusion protein (CTLA4Igp), which has a human glycan structure and is produced according to the present invention, exhibits an improved in vivo half life as compared to conventional plant-derived proteins, due to the presence of a human-like glycan structure. Consequently, the present invention using the plant expression system enables low-cost mass production of a CTLA4Igp fusion protein having an immunosuppressive activity comparable to that of the CTLA4IgM fusion protein expressed in conventional animal cell expression systems.
摘要翻译：本发明提供了含有人和重组1,4-半乳糖基转移酶基因的重组载体pBI-3D-hGalT或pBI-35S-hGalT; 用含有细胞毒性T淋巴细胞抗A型免疫球蛋白（CTLA4Ig）融合蛋白基因和重组载体pBI-3D-hGalT或pBI-355-hGalT的重组载体pMYN414转化的细胞系; 以及使用其使用具有人聚糖结构的植物来源的重组人CTLA4Ig（CTLA4Igp）融合蛋白的方法。具有人聚糖结构并根据本发明生产的植物细胞衍生的重组人CTLA4Ig融合蛋白（CTLA4Igp）与常规植物衍生的蛋白质相比显示出与常规植物衍生的蛋白质相比改善的体内半衰期，人类聚糖结构。因此，使用植物表达系统的本发明能够低成本大规模生产具有与常规动物细胞表达系统中表达的CTLA4IgM融合蛋白相当的免疫抑制活性的CTLA4Igp融合蛋白。

3. 发明申请

US20090181428A1 METHOD FOR PRODUCING TARGET PROTEINS USING AMINO ACIDS AND PYRUVIC ACIDS IN CULTURE OF PLANT CELLS 失效
标题翻译：在植物细胞培养中使用氨基酸和吡啶酸生产目标蛋白的方法
公开(公告)号：US20090181428A1
公开(公告)日：2009-07-16
申请号：US12160049
申请日：2007-09-19
申请人： Sang-Lin Kim , Hyun-Kwang Tan , Sang-Min Lim , Wuk-Sang Ryu , Hahn-Sun Jung , Song-Jae Lee , Cheon-Ik Park , Seung-Hoon Kang , Dong-Il Kim
发明人： Sang-Lin Kim , Hyun-Kwang Tan , Sang-Min Lim , Wuk-Sang Ryu , Hahn-Sun Jung , Song-Jae Lee , Cheon-Ik Park , Seung-Hoon Kang , Dong-Il Kim
IPC分类号： C12P21/00
CPC分类号： C12P21/00 , A01H4/00 , C12N15/8237
摘要： Provided is a method for producing a target protein via cultivation of transgenic plant cells comprising a promoter capable of expressing the protein under sugar-free conditions or in response to the depletion of sugar and a gene encoding the target protein, without exchange of a cell growth medium with a sugar-depleted medium. The method comprises 1) culturing transgenic plant cells in a sugar-rich medium to grow plant cells; and 2) culturing the transgenic plant cells with addition of an amino acid mixture to the culture of Step 1 without exchange of a cell growth medium with a sugar-depleted medium, thereby expressing a target protein. The method of the present invention enables commercial-scale production of recombinant proteins via establishment of optimized culture conditions by addition of an amino acid mixture to induce protein expression without exchange of a cell growth medium with a sugar-free medium, and addition of pyruvic acid during the induction period of protein expression to thereby enhance the production yield of target proteins.
摘要翻译：提供了通过培养转基因植物细胞来生产靶蛋白的方法，所述转基因植物细胞包含能够在无糖条件下表达该蛋白质的启动子，或者响应于糖的消耗和编码靶蛋白质的基因而不交换细胞生长培养基含糖耗竭的培养基。该方法包括：1）在富含糖的培养基中培养转基因植物细胞以生长植物细胞; 和2）向步骤1的培养物中加入氨基酸混合物的转基因植物细胞培养，而不用无糖培养基交换细胞生长培养基，从而表达靶蛋白。本发明的方法可以通过添加氨基酸混合物建立优化的培养条件来产生重组蛋白质，从而诱导蛋白质表达而不用无糖培养基交换细胞生长培养基，并加入丙酮酸在蛋白质表达的诱导期，从而提高靶蛋白的产量。

4. 发明授权

US09218760B2 Driving circuit for liquid crystal display device and method for driving the same 有权
标题翻译：液晶显示装置的驱动电路及其驱动方法
公开(公告)号：US09218760B2
公开(公告)日：2015-12-22
申请号：US13314367
申请日：2011-12-08
申请人： Song-Jae Lee , Young-Ho Kim
发明人： Song-Jae Lee , Young-Ho Kim
IPC分类号： G06F3/038 , G09G3/20
CPC分类号： G09G3/2096 , G09G2310/08
摘要： A driving circuit for a liquid crystal display device includes a liquid crystal panel comprising a plurality of pixel areas to display an image; a data driver configured to drive data lines of the liquid crystal panel; a gate driver configured to drive gate lines of the liquid crystal panel; and a timing controller configured to generate an internal enable signal in an initial driving where an external power is applied, to control the gate and data drivers, and configured to control the gate and data drivers based on synchronization signals, after controlling the driving of the gate driver to be stopped for one frame period when at least one synchronization signals are input from outside.
摘要翻译：液晶显示装置的驱动电路包括：液晶面板，包括多个像素区域以显示图像; 配置为驱动液晶面板的数据线的数据驱动器; 栅极驱动器，被配置为驱动所述液晶面板的栅极线; 以及定时控制器，其被配置为在施加外部电力的初始驱动中产生内部使能信号，以控制所述栅极和数据驱动器，并且被配置为在控制所述栅极和数据驱动器的驱动之后基于同步信号来控制所述栅极和数据驱动器当从外部输入至少一个同步信号时，门驱动器停止一帧周期。

5. 发明授权

US08749467B2 Liquid crystal display device using different methods according to type of image signals and method of driving the same 有权
标题翻译：液晶显示装置根据图像信号的类型使用不同的方法和驱动方法
公开(公告)号：US08749467B2
公开(公告)日：2014-06-10
申请号：US12838651
申请日：2010-07-19
申请人： Song-Jae Lee , Young-Ho Kim
发明人： Song-Jae Lee , Young-Ho Kim
IPC分类号： G09G3/36
CPC分类号： G09G3/3614 , G09G3/3648 , G09G2320/0242 , G09G2320/0613
摘要： A liquid crystal display device includes: a liquid crystal panel that displays images using a plurality of pixels each including red, green and blue sub-pixels; a gate driving portion that supplies a gate signal to the liquid crystal panel; a data driving portion that supplies a data signal to the liquid crystal panel; and a timing control portion that compares difference of gray level between image signals corresponding to the red, green and blue sub-pixels with a first threshold value and compares difference of gray level between the image signals corresponding to the red, green and blue sub-pixels of neighboring pixels of the plurality of pixels in order to judge type of the image signals, and drives the data driving portion in different methods according to the type of the image signals.
摘要翻译：液晶显示装置包括：液晶面板，其使用包括红色，绿色和蓝色子像素的多个像素显示图像; 栅极驱动部，其向液晶面板提供栅极信号; 将数据信号提供给液晶面板的数据驱动部; 以及定时控制部分，其将与红色，绿色和蓝色子像素相对应的图像信号之间的灰度差与第一阈值进行比较，并且将红色，绿色和蓝色子像素对应的图像信号之间的灰度差进行比较，以便判断图像信号的类型，并根据图像信号的类型以不同的方法驱动数据驱动部分。

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