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1. 发明授权

US08852898B2 L-threonine overproducing microorganism and method for preparing L-threonine using the same 有权
标题翻译： L-苏氨酸过量生产微生物和使用其制备L-苏氨酸的方法
公开(公告)号：US08852898B2
公开(公告)日：2014-10-07
申请号：US12160126
申请日：2007-07-02
申请人： Sang Yup Lee , Kwang Ho Lee , Jin Hwan Park , Tae Yong Kim
发明人： Sang Yup Lee , Kwang Ho Lee , Jin Hwan Park , Tae Yong Kim
IPC分类号： C12P13/08
CPC分类号： C12P13/08
摘要： The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified.
摘要翻译：本发明涉及使用位点特异性突变而不是任意突变（例如用突变诱导剂处理）制备高产量的L-苏氨酸的高浓度的突变型微生物，其制备方法和使用产生L-苏氨酸的突变体微生物制备L-苏氨酸。通过使用根据本发明的突变微生物，可以高产率制备L-苏氨酸，由于可以鉴定L-苏氨酸生产微生物的遗传信息，所以可以进行附加应变发展，并且可以容易地理解其生理现象。

2. 发明授权

US08148137B2 Mutant microorganism having improved production ability of branched amino acid and method for preparing branched amino acid using the same 有权
标题翻译：具有提高支链氨基酸生产能力的突变体微生物及使用其制备支链氨基酸的方法
公开(公告)号：US08148137B2
公开(公告)日：2012-04-03
申请号：US12097099
申请日：2007-03-14
申请人： Sang Yup Lee , Jin Hwan Park , Kwang Ho Lee , Tae Yong Kim
发明人： Sang Yup Lee , Jin Hwan Park , Kwang Ho Lee , Tae Yong Kim
IPC分类号： C12N1/21 , C12P13/08
CPC分类号： C12P13/08 , C12N9/1014 , C12Y201/02011
摘要： The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site-specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.
摘要翻译：本发明涉及具有提高的支链氨基酸生产率的突变微生物，以及使用突变微生物生产支链氨基酸的方法。更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

3. 发明申请

US20090053779A1 MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME 有权
标题翻译：具有改进的分支氨基酸生产能力的突变微生物及使用其制备分支氨基酸的方法
公开(公告)号：US20090053779A1
公开(公告)日：2009-02-26
申请号：US12097099
申请日：2007-03-14
申请人： Sang Yup Lee , Jin Hwan Park , Kwang Ho Lee , Tae Yong Kim
发明人： Sang Yup Lee , Jin Hwan Park , Kwang Ho Lee , Tae Yong Kim
IPC分类号： C12P13/08 , C12N15/01 , C12N15/80 , C12N15/81 , C12N15/74 , C12N1/14 , C12N1/15 , C12N1/16 , C12N1/19 , C12N1/20 , C12N1/21
CPC分类号： C12P13/08 , C12N9/1014 , C12Y201/02011
摘要： The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site-specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.
摘要翻译：本发明涉及具有提高的支链氨基酸生产率的突变微生物，以及使用突变微生物生产支链氨基酸的方法。更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

4. 发明申请

US20110003349A1 L-Threonine Overproducing Microorganism and Method for Preparing L-Threonine Using the Same 有权
标题翻译： L-苏氨酸过量生产微生物及使用其制备L-苏氨酸的方法
公开(公告)号：US20110003349A1
公开(公告)日：2011-01-06
申请号：US12160126
申请日：2007-07-02
申请人： Sang Yup Lee , Kwang Ho Lee , Jin Hwan Park , Tae Yong Kim
发明人： Sang Yup Lee , Kwang Ho Lee , Jin Hwan Park , Tae Yong Kim
IPC分类号： C12P13/08 , C12N15/63 , C12N1/21 , C12N1/15 , C12N1/19
CPC分类号： C12P13/08
摘要： The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified.
摘要翻译：本发明涉及使用位点特异性突变而不是任意突变（例如用突变诱导剂处理）制备高产量的L-苏氨酸的高浓度的突变型微生物，其制备方法和使用产生L-苏氨酸的突变体微生物制备L-苏氨酸。通过使用根据本发明的突变微生物，可以高产率制备L-苏氨酸，由于可以鉴定L-苏氨酸生产微生物的遗传信息，所以可以进行附加应变发展，并且可以容易地理解其生理现象。

5. 发明申请

US20100143911A1 METHOD FOR SCREENING ESSENTIAL METABOLITES IN GROWTH OF MICROORGANISMS 有权
标题翻译：筛选微生物生长中基本代谢物的方法
公开(公告)号：US20100143911A1
公开(公告)日：2010-06-10
申请号：US12519791
申请日：2007-12-21
申请人： Sang Yup Lee , Hawoong Jeong , Tae Yong Kim , Pan-Jun Kim , Kwang Ho Lee
发明人： Sang Yup Lee , Hawoong Jeong , Tae Yong Kim , Pan-Jun Kim , Kwang Ho Lee
IPC分类号： C12Q1/68 , C12Q1/02 , C12N15/74 , C12N1/21
CPC分类号： C12Q1/18 , G01N33/5038
摘要： The present invention disclosed is a method for screening metabolites essential for the growth of microorganism using metabolic flux analysis. More specifically, the present invention relates to the method for screening metabolites essential for the growth of microorganism, by selecting a target microorganism, constructing a metabolic network model of the selected microorganism, inactivating the consumption reaction of each of metabolites in the constructed metabolic network model, analyzing the metabolic flux of the metabolites to select metabolites essential for the growth of the microorganism, and confirming the selected metabolites using the utilization of each of the metabolites, defined as flux sum (Φ). According to the present invention, metabolites essential for the growth of microorganism, and genes involved in the essential metabolites, can be screened in a convenient manner, and drug-target genes against pathogenic microorganisms can be predicted by deleting genes associated with the metabolites screened according to the method.
摘要翻译：所公开的本发明是使用代谢通量分析来筛选微生物生长所必需的代谢物的方法。更具体地说，本发明涉及通过选择靶微生物，构建所选择的微生物的代谢网络模型，在构建的代谢网络模型中灭活各代谢物的消费反应来筛选微生物生长所必需的代谢物的方法分析代谢物的代谢通量以选择微生物生长所必需的代谢物，并使用定义为通量和（Φ）的每种代谢物来确认所选择的代谢物。根据本发明，可以以便利的方式筛选微生物生长所必需的代谢物和基本代谢物所涉及的基因，并且可以通过删除与代谢物相关的基因来预测针对病原微生物的药物靶基因的方法。

6. 发明授权

US08494779B2 Method for screening essential metabolites in growth of microorganisms 有权
标题翻译：筛选微生物生长中重要代谢物的方法
公开(公告)号：US08494779B2
公开(公告)日：2013-07-23
申请号：US12519791
申请日：2007-12-21
申请人： Sang Yup Lee , Hawoong Jeong , Tae Yong Kim , Pan-Jun Kim , Kwang Ho Lee
发明人： Sang Yup Lee , Hawoong Jeong , Tae Yong Kim , Pan-Jun Kim , Kwang Ho Lee
IPC分类号： G06F19/10 , G06F19/12
CPC分类号： C12Q1/18 , G01N33/5038
摘要： The present invention disclosed is a method for screening metabolites essential for the growth of microorganism using metabolic flux analysis. More specifically, the present invention relates to the method for screening metabolites essential for the growth of microorganism, by selecting a target microorganism, constructing a metabolic network model of the selected microorganism, inactivating the consumption reaction of each of metabolites in the constructed metabolic network model, analyzing the metabolic flux of the metabolites to select metabolites essential for the growth of the microorganism, and confirming the selected metabolites using the utilization of each of the metabolites, defined as flux sum (Φ). According to the present invention, metabolites essential for the growth of microorganism, and genes involved in the essential metabolites, can be screened in a convenient manner, and drug-target genes against pathogenic microorganisms can be predicted by deleting genes associated with the metabolites screened according to the method.
摘要翻译：所公开的本发明是使用代谢通量分析来筛选微生物生长所必需的代谢物的方法。更具体地说，本发明涉及通过选择靶微生物，构建所选择的微生物的代谢网络模型，在构建的代谢网络模型中灭活各代谢物的消费反应来筛选微生物生长所必需的代谢物的方法分析代谢物的代谢通量以选择对于微生物生长至关重要的代谢物，并且使用定义为通量总和（Phi）的每种代谢物来确认所选择的代谢物。根据本发明，可以以便利的方式筛选微生物生长所必需的代谢物和基本代谢物所涉及的基因，并且可以通过删除与代谢物相关的基因来预测针对病原微生物的药物靶基因的方法。

7. 发明申请

US20130017588A1 RECOMBINANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE BUTANOL AND METHOD OF PRODUCING BUTANOL USING THE SAME 有权
标题翻译：具有生产丁醇的能力提高的重组微生物和使用其的丁醇的生产方法
公开(公告)号：US20130017588A1
公开(公告)日：2013-01-17
申请号：US13497743
申请日：2010-09-21
申请人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
发明人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
IPC分类号： C12N1/21 , C12P7/16 , C12N15/01
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y203/01008 , C12Y203/01019 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.
摘要翻译：本发明涉及生产丁醇的能力提高的重组微生物，以及使用其制备丁醇的方法。更具体地，本发明涉及通过操作其代谢网络来增加生产丁醇的能力的重组微生物，以及使用其生产丁醇的方法。在具有编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中，具有增加的产生丁醇能力的重组微生物包括编码转化乙酰CoA至乙酸的酶的基因的缺失。通过操作微生物的代谢通过获得的重组微生物能够高效地选择性地生产丁醇，因此可用作生产工业溶剂和运输燃料的微生物。

8. 发明授权

US09096872B2 Recombinant microorganisms having increased ability to produce butanol and method of producing butanol using the same 有权
标题翻译：具有提高丁醇生产能力的重组微生物和使用其生产丁醇的方法
公开(公告)号：US09096872B2
公开(公告)日：2015-08-04
申请号：US13497743
申请日：2010-09-21
申请人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
发明人： Sang Yup Lee , Yu-Sin Jang , Jin Young Lee , Joung Min Lee , Jin Hwan Park
IPC分类号： C12N1/21 , C12P7/16 , C12N9/04 , C12N9/10
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y203/01008 , C12Y203/01019 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.
摘要翻译：本发明涉及生产丁醇的能力提高的重组微生物，以及使用其制备丁醇的方法。更具体地，本发明涉及通过操作其代谢网络来增加生产丁醇的能力的重组微生物，以及使用其生产丁醇的方法。在具有编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中，具有增加的产生丁醇能力的重组微生物包括编码转化乙酰CoA至乙酸的酶的基因的缺失。通过操作微生物的代谢通过获得的重组微生物能够以高效率选择性地生产丁醇，因此可用作生产工业溶剂和运输燃料的微生物。

9. 发明申请

US20100136640A1 ENHANCED BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING BUTANOL USING THE SAME 审中-公开
标题翻译：生产微生物的增强的丁醇和使用它们制备丁醇的方法
公开(公告)号：US20100136640A1
公开(公告)日：2010-06-03
申请号：US12519060
申请日：2007-12-14
申请人： Sang Yup Lee , Jin Hwan Park , Eleftherios Terry Papoutsakis
发明人： Sang Yup Lee , Jin Hwan Park , Eleftherios Terry Papoutsakis
IPC分类号： C12P7/16 , C12N1/21 , C12N1/19 , C12N1/15
CPC分类号： C12P7/16 , Y02E50/10
摘要： The present invention relates to a recombinant mutant microorganism having enhanced butanol producing capacity and a method for producing butanol using the same. In the microorganism, genes coding for enzymes responsible for the biosynthesis of lactate, ethanol and/or acetate are deleted or attenuated and genes coding for enzymes involved in butanol biosynthesis are introduced and amplified.
摘要翻译：本发明涉及具有提高的丁醇生产能力的重组突变体微生物和使用其的丁醇生产方法。在微生物中，编码负责乳酸盐，乙醇和/或乙酸盐的生物合成的酶的基因被缺失或减弱，并且引入并扩增了参与丁醇生物合成的酶编码基因。

10. 发明申请

US20110269183A1 RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE 审中-公开
标题翻译：具有使用蔗糖作为碳源的能力的重组微生物
公开(公告)号：US20110269183A1
公开(公告)日：2011-11-03
申请号：US12808533
申请日：2008-12-18
申请人： Sang Yup Lee , Jeong Wook Lee , Hyohak Song , Ji Mahn Kim , Sol Choi , Jin Hwan Park
发明人： Sang Yup Lee , Jeong Wook Lee , Hyohak Song , Ji Mahn Kim , Sol Choi , Jin Hwan Park
IPC分类号： C12P21/00 , C12N9/26 , C12N15/63 , C12N15/56 , C12N1/19 , C12N1/21 , C12P1/04 , C12P1/02 , C12N9/12 , C12N1/15
CPC分类号： C12N15/70 , C12N1/20 , C12N9/1205 , C12N9/16 , C12N9/2408 , C12P1/04 , C12P7/065 , C12P7/40 , C12P7/46 , C12P7/54 , C12P7/56 , C12P13/08 , C12Y207/01004 , C12Y207/01069 , C12Y302/01026 , Y02E50/17
摘要： The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.
摘要翻译：本发明涉及能够代谢蔗糖的重组微生物，更具体地说，涉及能够代谢蔗糖的重组微生物，其中导入编码蔗糖磷酸转移酶和/或编码蔗糖-6-磷酸水解酶的基因的基因或重组微生物能够代谢其中引入编码“呋喃果糖苷酶”的基因的蔗糖。根据本发明，提供了能够使用廉价的蔗糖作为碳源代替昂贵的葡萄糖的重组微生物。此外，在不能使用蔗糖作为碳源的微生物的培养方法中，蔗糖可以替代包括葡萄糖在内的其它碳源。

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