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1. 发明申请

US20180079788A1 Constitutive Yeast LLP Promotor-Based Expression Systems 审中-公开
公开(公告)号：US20180079788A1
公开(公告)日：2018-03-22
申请号：US15554552
申请日：2016-03-02
申请人： Sandoz AG
发明人： Clemens Achmüller , Ferdinand Zepeck , Franz Hartner , Thomas Specht
IPC分类号： C07K14/39 , C12N15/81
CPC分类号： C07K14/39 , C07K14/395 , C12N15/81
摘要： The present invention belongs to the field of biotechnology, recombinant protein production, molecular biology, microbiology and microbial genetics. It provides a modified eukaryotic cell that is modified to the effect that the modified eukaryotic cell is not able to provide an SSN6-like related protein or an SSN6-like protein that exerts its wildtype function and/or wildtype activity, the amount of SSN6-like related protein or of SSN6-like protein being present in the modified eukaryotic cell differs from the amount of SSN6-like related protein or of SSN6-like protein being present in its wildtype form, and/or essentially no SSN6-like related protein or SSN6-like protein is present in the modified cell. Additionally, the present invention provides a polynucleotide sequence comprising a modified ssn6-like related gene or modified ssn6-like gene, and a vector comprising said polynucleoptide. Additionally provided is an expression vector comprising a promoter that is repressed in the presence of SSN6-like protein or SSN6-like related protein, and a host cell comprising said vectors. The present invention further refers to a method for determining the purity of a composition by using the modified eukaryotic cell, to a method of expressing gene(s) of interest, and eukaryotic cells comprising modified ssn6-like related gene or ssn6-like gene.

2. 发明授权

US11203620B2 Constitutive yeast LLP promotor-based expression systems 有权
公开(公告)号：US11203620B2
公开(公告)日：2021-12-21
申请号：US15554552
申请日：2016-03-02
申请人： Sandoz AG
发明人： Clemens Achmüller , Ferdinand Zepeck , Franz Hartner , Thomas Specht
IPC分类号： C07K14/39 , C12N15/81 , C07K14/395
摘要： The present invention provides a modified eukaryotic cell wherein the modified eukaryotic cell is not able to provide an SSN6-like protein that exerts its wildtype function and/or wildtype activity, the amount of SSN6-like protein being present in the modified eukaryotic cell differs from the amount of SSN6-like protein being present in its wildtype form, and/or essentially no SSN6-like protein is present in the modified cell. Additionally, the present invention provides a polynucleotide sequence comprising a modified ssn6-like gene, and a vector comprising said polynucleoptide. Additionally provided is an expression vector comprising a promoter that is repressed in the presence of SSN6-like protein, and a host cell comprising said vectors. The present invention further refers to a method for determining the purity of a composition by using the modified eukaryotic cell, to a method of expressing gene(s) of interest, and eukaryotic cells comprising modified ssn6-like gene.

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