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1. 发明授权

US08969025B2 Protein electron mediator 有权
标题翻译：蛋白质电子介体
公开(公告)号：US08969025B2
公开(公告)日：2015-03-03
申请号：US12953816
申请日：2010-11-24
申请人： Ryo Takenaka , Michinari Honda , Hironori Omura
发明人： Ryo Takenaka , Michinari Honda , Hironori Omura
IPC分类号： C12Q1/54 , C07K14/00 , H01M8/16 , C07K14/80 , C12N9/04 , C12N15/62 , C12Q1/00 , A61B5/145 , A61B5/1486
CPC分类号： H01M8/16 , A61B5/14532 , A61B5/1486 , C07K14/80 , C12N9/0006 , C12N15/62 , C12Q1/004 , Y02E60/527
摘要： The problem to be resolved is to provide an electron mediator and a fusion body with high affinity with an enzyme, a measuring method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.The present invention relates to an electron mediator for glucose oxidoreductase comprising extracellular secretion type cytochrome, a fusion body in which the electron mediator is fused with glucose oxidoreductase, a composition for glucose measurement including the electron mediator or fusion body, a gene encoding a new extracellular secretion type cytochrome, and a measurement method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.
摘要翻译：要解决的问题是提供与酶具有高亲和力的电子介体和融合体，使用细胞外分泌型细胞色素的测定方法和酶，电极和传感器。本发明涉及包含细胞外分泌型细胞色素的葡萄糖氧化还原酶的电子介体，其中电子介体与葡萄糖氧化还原酶融合的融合体，包括电子介体或融合体的葡萄糖测量用组合物，编码新的细胞外基因的基因分泌型细胞色素，以及使用细胞外分泌型细胞色素和酶，电极和传感器的测定方法。

2. 发明授权

US08716442B2 Protein electron mediator 有权
标题翻译：蛋白质电子介体
公开(公告)号：US08716442B2
公开(公告)日：2014-05-06
申请号：US12857880
申请日：2010-08-17
申请人： Ryo Takenaka , Michinari Honda , Hironori Omura
发明人： Ryo Takenaka , Michinari Honda , Hironori Omura
IPC分类号： C07K14/00
CPC分类号： H01M8/16 , A61B5/14532 , A61B5/1486 , C07K14/80 , C12N9/0006 , C12N15/62 , C12Q1/004 , Y02E60/527
摘要： The problem to be resolved is to provide an electron mediator and a fusion body with high affinity with an enzyme, a measuring method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.The present invention relates to an electron mediator for glucose oxidoreductase comprising extracellular secretion type cytochrome, a fusion body in which the electron mediator is fused with glucose oxidoreductase, a composition for glucose measurement including the electron mediator or fusion body, a gene encoding a new extracellular secretion type cytochrome, and a measurement method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.
摘要翻译：要解决的问题是提供与酶具有高亲和力的电子介体和融合体，使用细胞外分泌型细胞色素的测定方法和酶，电极和传感器。本发明涉及包含细胞外分泌型细胞色素的葡萄糖氧化还原酶的电子介体，其中电子介体与葡萄糖氧化还原酶融合的融合体，包括电子介体或融合体的葡萄糖测量用组合物，编码新的细胞外基因的基因分泌型细胞色素，以及使用细胞外分泌型细胞色素和酶，电极和传感器的测定方法。

3. 发明申请

US20110045513A1 PROTEIN ELECTRON MEDIATOR 有权
标题翻译：蛋白电子介质
公开(公告)号：US20110045513A1
公开(公告)日：2011-02-24
申请号：US12857880
申请日：2010-08-17
申请人： Ryo TAKENAKA , Michinari Honda , Hironori Omura
发明人： Ryo TAKENAKA , Michinari Honda , Hironori Omura
IPC分类号： C12Q1/54 , C07K14/00 , C12N15/31 , C12N15/74 , C12N1/21 , C12P21/02 , C12N9/96 , C12N15/53 , C12N15/62 , C12Q1/26
CPC分类号： H01M8/16 , A61B5/14532 , A61B5/1486 , C07K14/80 , C12N9/0006 , C12N15/62 , C12Q1/004 , Y02E60/527
摘要： The problem to be resolved is to provide an electron mediator and a fusion body with high affinity with an enzyme, a measuring method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.The present invention relates to an electron mediator for glucose oxidoreductase comprising extracellular secretion type cytochrome, a fusion body in which the electron mediator is fused with glucose oxidoreductase, a composition for glucose measurement including the electron mediator or fusion body, a gene encoding a new extracellular secretion type cytochrome, and a measurement method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.
摘要翻译：要解决的问题是提供与酶具有高亲和力的电子介体和融合体，使用细胞外分泌型细胞色素的测定方法和酶，电极和传感器。本发明涉及包含细胞外分泌型细胞色素的葡萄糖氧化还原酶的电子介体，其中电子介体与葡萄糖氧化还原酶融合的融合体，包括电子介体或融合体的葡萄糖测量用组合物，编码新的细胞外基因的基因分泌型细胞色素，以及使用细胞外分泌型细胞色素和酶，电极和传感器的测定方法。

4. 发明申请

US20110136158A1 PROTEIN ELECTRON MEDIATOR 有权
公开(公告)号：US20110136158A1
公开(公告)日：2011-06-09
申请号：US12953816
申请日：2010-11-24
申请人： Ryo TAKENAKA , Michinari Honda , Hironori Omura
发明人： Ryo TAKENAKA , Michinari Honda , Hironori Omura
IPC分类号： C12Q1/54 , C12Q1/26 , C12Q1/32 , C12N9/96 , C12M1/34
CPC分类号： H01M8/16 , A61B5/14532 , A61B5/1486 , C07K14/80 , C12N9/0006 , C12N15/62 , C12Q1/004 , Y02E60/527
摘要： The problem to be resolved is to provide an electron mediator and a fusion body with high affinity with an enzyme, a measuring method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.The present invention relates to an electron mediator for glucose oxidoreductase comprising extracellular secretion type cytochrome, a fusion body in which the electron mediator is fused with glucose oxidoreductase, a composition for glucose measurement including the electron mediator or fusion body, a gene encoding a new extracellular secretion type cytochrome, and a measurement method using extracellular secretion type cytochrome and an enzyme, an electrode, and a sensor.

5. 发明申请

US20100323378A1 MODIFIED GLUCOSE DEHYDROGENASE GENE 有权
标题翻译：改良葡萄糖脱氢酶基因
公开(公告)号：US20100323378A1
公开(公告)日：2010-12-23
申请号：US12810213
申请日：2008-12-26
申请人： Michinari Honda , Ryo Takenaka , Fuminao Kobayashi
发明人： Michinari Honda , Ryo Takenaka , Fuminao Kobayashi
IPC分类号： C12Q1/54 , C12N9/04 , C07H21/04 , C12N15/63 , C12N1/21 , C12N1/15
CPC分类号： C12Q1/32 , C12N9/0006 , C12Q1/006
摘要： [Object] To provide an FAD-conjugated glucose dehydrogenase having a higher specificity for glucose.[Means for Resolution] A modified glucose dehydrogenase (GLD) which includes a substitution of at least one amino acid residue selected from the group consisting of amino acid residues at positions 37, 69, 72, 73, 76, 78, 102, 217, 228, 240, 356, 407, 424, 437, 527, and 530 in an amino acid sequence of a wild-type FAD-conjugated glucose dehydrogenase (GLD) represented by SEQ ID NO: 1, and has a decreased ratio of activity for xylose/activity for glucose as compared with the wild-type GLD; a polynucleotide encoding the modified GLD; a recombinant vector containing the polynucleotide; a transformed cell produced by using the recombinant vector; etc.
摘要翻译：提供对葡萄糖具有较高特异性的FAD-缀合的葡萄糖脱氢酶。 [解决方法]一种修饰的葡萄糖脱氢酶（GLD），其包括至少一个氨基酸残基的取代，所述氨基酸残基选自第37,69,72,73,76,78,102,217，在由SEQ ID NO：1表示的野生型FAD-缀合的葡萄糖脱氢酶（GLD）的氨基酸序列中的228,240,356,407,424,473,527和530，并且具有降低的与野生型GLD相比，葡萄糖的木糖/活性; 编码经修饰的GLD的多核苷酸; 含有多核苷酸的重组载体; 通过使用重组载体产生的转化细胞; 等等

6. 发明授权

US08445221B2 Modified glucose dehydrogenase gene 有权
标题翻译：改良葡萄糖脱氢酶基因
公开(公告)号：US08445221B2
公开(公告)日：2013-05-21
申请号：US12810213
申请日：2008-12-26
申请人： Michinari Honda , Ryo Takenaka , Fuminao Kobayashi
发明人： Michinari Honda , Ryo Takenaka , Fuminao Kobayashi
IPC分类号： C12Q1/54 , C12N9/00 , C12N9/02 , C12N1/20 , C07H21/04
CPC分类号： C12Q1/32 , C12N9/0006 , C12Q1/006
摘要： [Object] To provide an FAD-conjugated glucose dehydrogenase having a higher specificity for glucose.[Means for Resolution] A modified glucose dehydrogenase (GLD) which includes a substitution of at least one amino acid residue selected from the group consisting of amino acid residues at positions 37, 69, 72, 73, 76, 78, 102, 217, 228, 240, 356, 407, 424, 437, 527, and 530 in an amino acid sequence of a wild-type FAD-conjugated glucose dehydrogenase (GLD) represented by SEQ ID NO: 1, and has a decreased ratio of activity for xylose/activity for glucose as compared with the wild-type GLD; a polynucleotide encoding the modified GLD; a recombinant vector containing the polynucleotide; a transformed cell produced by using the recombinant vector; etc.
摘要翻译：提供对葡萄糖具有较高特异性的FAD-缀合的葡萄糖脱氢酶。 [解决方法]一种修饰的葡萄糖脱氢酶（GLD），其包括至少一个氨基酸残基的取代，所述氨基酸残基选自第37,69,72,73,76,78,102,217，在由SEQ ID NO：1表示的野生型FAD-缀合的葡萄糖脱氢酶（GLD）的氨基酸序列中的228,240,356,407,424,473,527和530，并且具有降低的与野生型GLD相比，葡萄糖的木糖/活性; 编码经修饰的GLD的多核苷酸; 含有多核苷酸的重组载体; 通过使用重组载体产生的转化细胞; 等等

7. 发明申请

US20110033880A1 FAD-CONJUGATED GLUCOSE DEHYDROGENASE GENE 有权
标题翻译： FAD-结合葡萄糖脱氢酶基因
公开(公告)号：US20110033880A1
公开(公告)日：2011-02-10
申请号：US12866071
申请日：2007-06-29
申请人： Takako Yada , Koji Miyamoto , Michinari Honda
发明人： Takako Yada , Koji Miyamoto , Michinari Honda
IPC分类号： C12Q1/54 , C07H21/00 , C12N15/63 , C12N1/21 , C12N1/00 , C12N1/15 , C12N9/96
CPC分类号： C12N9/0006 , C12Q1/006 , C12Q1/32 , C12Q1/54 , C12Y101/9901 , G01N27/301 , G01N27/302 , G01N27/3271 , G01N33/66 , G01N2333/902 , G01N2333/904 , Y10T29/49117
摘要： An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.
摘要翻译：本发明的目的是提供：编码与葡萄糖具有优异的反应性，优异的热稳定性和优异的底物识别性能的优异性质的FAD-共轭葡萄糖脱氢酶的新型基因（多核苷酸），还具有低活性麦芽糖使用用该基因转染的转化体细胞来生产酶的方法; 和测定葡萄糖的方法，用于测定葡萄糖的试剂组合物，用于测定葡萄糖的生物传感器等，其特征在于使用所得的酶。本发明涉及编码FAD-缀合的葡萄糖脱氢酶的多核苷酸，其包含含有氨基酸序列的多肽：X1-X2-X3-X4-X5-X6（其中X1和X2独立地代表脂族氨基酸残基; X3和X6 独立地表示支链氨基酸残基; X4和X5独立地表示杂环氨基酸残基或芳香族氨基酸残基）; 和别的。

8. 发明授权

US08969060B2 Modified glucose dehydrogenase 有权
标题翻译：改良葡萄糖脱氢酶
公开(公告)号：US08969060B2
公开(公告)日：2015-03-03
申请号：US13598944
申请日：2012-08-30
申请人： Michinari Honda , Tsuyoshi Kameda , Fuminao Kobayashi
发明人： Michinari Honda , Tsuyoshi Kameda , Fuminao Kobayashi
IPC分类号： C12N9/04 , C12Q1/32 , C07K14/00 , C12P21/00
CPC分类号： C12N9/0006 , C12Y101/9901
摘要： The purpose of the present invention is to provide an FAD-conjugated glucose dehydrogenase that is hard to be inhibited by the inhibitors such as 1,10-phenanthroline.The present invention relates to a modified glucose dehydrogenase (GLD), comprising an amino acid sequence of a wild-type FAD-conjugated glucose dehydrogenase (GLD) represented by SEQ ID NO: 1 having a substitution of at least one amino acid residue selected from the group consisting of amino acid residues at positions 298, 338, 340, 341, 343, 352, 354, 424, 426, 431 and 432, wherein the modified GLD has a reduced susceptibility to an inhibitor, as compared with the wild-type GLD, especially to said modified GLD, which has 40% or more of a relative activity when determined in a system wherein the inhibitor coexists at a final concentration of 1 mM based on an enzymatic activity when determined in a system wherein the inhibitor does not coexist.
摘要翻译：本发明的目的是提供难以被诸如1,10-菲咯啉的抑制剂抑制的FAD-缀合的葡萄糖脱氢酶。本发明涉及一种修饰的葡萄糖脱氢酶（GLD），其包含由SEQ ID NO：1表示的野生型FAD-缀合的葡萄糖脱氢酶（GLD）的氨基酸序列，其具有至少一个选自以下的氨基酸残基：由298,338,340,341,343,352,354,424,426,431和432位的氨基酸残基组成，其中与野生型相比，修饰的GLD对抑制剂的易感性降低 GLD，特别是所述修饰的GLD，当在其中抑制剂不共存的系统中测定时，当其在抑制剂基于酶活性的最终浓度为1mM共存时，其具有40％或更高的相对活性。

9. 发明授权

US08492130B2 FAD-conjugated glucose dehydrogenase gene 有权
标题翻译： FAD-共轭葡萄糖脱氢酶基因
公开(公告)号：US08492130B2
公开(公告)日：2013-07-23
申请号：US12866071
申请日：2007-06-29
申请人： Takako Yada , Koji Miyamoto , Michinari Honda
发明人： Takako Yada , Koji Miyamoto , Michinari Honda
IPC分类号： C12Q1/54 , C12N9/00 , C12N9/96 , C12N1/20 , C12N15/00
CPC分类号： C12N9/0006 , C12Q1/006 , C12Q1/32 , C12Q1/54 , C12Y101/9901 , G01N27/301 , G01N27/302 , G01N27/3271 , G01N33/66 , G01N2333/902 , G01N2333/904 , Y10T29/49117
摘要： An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.
摘要翻译：本发明的目的是提供：编码与葡萄糖具有优异的反应性，优异的热稳定性和优异的底物识别性能的优异性质的FAD-共轭葡萄糖脱氢酶的新型基因（多核苷酸），还具有低活性麦芽糖使用用该基因转染的转化体细胞来生产酶的方法; 和测定葡萄糖的方法，用于测定葡萄糖的试剂组合物，用于测定葡萄糖的生物传感器等，其特征在于使用所得的酶。本发明涉及编码FAD-缀合的葡萄糖脱氢酶的多核苷酸，其包含含有氨基酸序列的多肽：X1-X2-X3-X4-X5-X6（其中X1和X2独立地表示脂肪族氨基酸残基; X3和X6 独立地表示支链氨基酸残基; X4和X5独立地表示杂环氨基酸残基或芳香族氨基酸残基）; 和别的。

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