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1. 发明授权

US07163658B2 Rapid sequencing of polymers 有权
标题翻译：聚合物快速测序
公开(公告)号：US07163658B2
公开(公告)日：2007-01-16
申请号：US10421343
申请日：2003-04-23
申请人： Rouvain Bension
发明人： Rouvain Bension
IPC分类号： G01N15/06 , G01N33/00 , G01N33/48 , C21Q1/68
CPC分类号： C12Q1/6869 , C12Q2565/601 , C12Q2565/519 , C12Q2523/307
摘要： A method and device for sequencing at least a fragment of a linear polymer. The device comprises a well for placement of a rotaxane comprising the combination of a cyclic molecule and a linear polymer threaded through said cyclic molecule; a probe having the ability to move the linear polymer relative to the cyclic molecule while producing a signal resulting from the interaction of the cyclic molecule and a unit attached to the polymer; and means for reading said signal. The process comprises formation of the rotaxane, attachment of the probe, movement of the cyclic molecule relative to the linear polymer and the reading of signals. The device and method are especially useful for the sequencing of DNA.
摘要翻译：用于对至少一个线性聚合物的片段进行测序的方法和装置。该装置包括用于放置轮烷的孔，该轮烷包含环状分子和穿过所述环状分子的线性聚合物的组合; 探针具有相对于环状分子移动线性聚合物的能力，同时产生由环状分子与连接于聚合物的单元相互作用而产生的信号; 以及用于读取所述信号的装置。该方法包括形成轮烷，探针的附着，环状分子相对于线性聚合物的移动和信号的读取。该装置和方法对DNA的测序特别有用。

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