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1. 发明申请

US20110014710A1 COMPOSITIONS, METHODS AND KITS FOR BIARSENICAL FLUOROPHORE LABELING 有权
标题翻译： BIARSENICAL FLOOROPHORE标签的组成，方法和工具
公开(公告)号：US20110014710A1
公开(公告)日：2011-01-20
申请号：US12510995
申请日：2009-07-28
申请人： Roumen A. Bogoev , Joseph W. Amshey , George Hanson
发明人： Roumen A. Bogoev , Joseph W. Amshey , George Hanson
IPC分类号： G01N33/50 , C07K1/13 , G01N1/00 , G01N27/447 , G01N33/559
CPC分类号： C12Y302/01031 , C07K1/13 , C12N9/1033 , C12N9/2442 , C12Y302/01014 , G01N33/533 , Y10T436/105831 , Y10T436/13 , Y10T436/18 , Y10T436/25
摘要： Methods, compositions, and kits for labeling tetracysteine-tagged proteins with biarsenical fluorophores with increased specificity, including compositions, methods and kits particularly adapted for labeling of tetracysteine-tagged proteins to be resolved within an electrophoresis gel.
摘要翻译：用于用具有增加的特异性的双缩氟荧光团标记四半胱氨酸标记的蛋白质的方法，组合物和试剂盒，包括组合物，方法和试剂盒，特别适用于在电泳凝胶内标记待分解的四半胱氨酸标记的蛋白质。

2. 发明申请

US20120184712A1 HOMOGENOUS POPULATIONS OF MOLECULES 审中-公开
标题翻译：均质的分子群体
公开(公告)号：US20120184712A1
公开(公告)日：2012-07-19
申请号：US13329883
申请日：2011-12-19
申请人： Joseph W. Amshey , Roumen A. Bogoev
发明人： Joseph W. Amshey , Roumen A. Bogoev
IPC分类号： C07K14/00
CPC分类号： G01N33/6842 , C40B40/10 , G01N2496/00
摘要： The invention provides populations of molecules that are prepared as, or treated to become, homogeneous for one or more molecular characteristics. In an aspect, the invention relates to molecular weight standards that may be used to determine the molecular weight or apparent molecular weight of uncharacterized molecules, such as proteins and nucleic acids, as well as in other applications. In one aspect, the molecular weight standards are pre-stained.
摘要翻译：本发明提供了为一种或多种分子特征制备或被处理成均匀的分子群体。在一方面，本发明涉及可用于测定未表征的分子（例如蛋白质和核酸）以及其它应用的分子量或表观分子量的分子量标准品。在一个方面，分子量标准物被预染色。

3. 发明授权

US09523692B2 Homogenous populations of molecules 有权
标题翻译：均质群体的分子
公开(公告)号：US09523692B2
公开(公告)日：2016-12-20
申请号：US13329883
申请日：2011-12-19
申请人： Joseph W. Amshey , Roumen A. Bogoev
发明人： Joseph W. Amshey , Roumen A. Bogoev
IPC分类号： G01N33/68 , C40B40/10
CPC分类号： G01N33/6842 , C40B40/10 , G01N2496/00
摘要： The invention provides populations of molecules that are prepared as, or treated to become, homogeneous for one or more molecular characteristics. In an aspect, the invention relates to molecular weight standards that may be used to determine the molecular weight or apparent molecular weight of uncharacterized molecules, such as proteins and nucleic acids, as well as in other applications. In one aspect, the molecular weight standards are pre-stained.
摘要翻译：本发明提供了为一种或多种分子特征制备或被处理成均匀的分子群体。在一方面，本发明涉及可用于测定未表征的分子（例如蛋白质和核酸）以及其它应用的分子量或表观分子量的分子量标准品。在一个方面，分子量标准物被预染色。

4. 发明申请

US20100105576A1 Three Frame cDNA Expression Libraries for Functional Screening of Proteins 审中-公开
标题翻译：用于功能筛选蛋白质的三帧cDNA表达文库
公开(公告)号：US20100105576A1
公开(公告)日：2010-04-29
申请号：US12552986
申请日：2009-09-02
申请人： Thomas R. Jackson , Adam S. Henry , Joseph W. Amshey , Roumen A. Bogoev
发明人： Thomas R. Jackson , Adam S. Henry , Joseph W. Amshey , Roumen A. Bogoev
IPC分类号： C40B50/06 , C40B50/00 , C07H21/00
CPC分类号： C12N15/1093 , C12N15/1086
摘要： Compositions and methods are provided for generating three frame cDNA expression libraries for functional screening of proteins. The invention includes sets of 5′ adapters for cloning cDNA molecules in which the sets include three adapters that can be used to clone a particular cDNA in all three reading frames. The libraries so generated have greater complexity of expressed open reading frames, and thus can improve the success of functional protein screens, such as two hybrid screens. The adapters also have recombination sites for efficient cloning and transfer between systems.
摘要翻译：提供组合物和方法用于产生用于蛋白质功能筛选的三个框架cDNA表达文库。本发明包括用于克隆cDNA分子的5'衔接子的集合，其中所述组包括可用于在所有三个阅读框中克隆特定cDNA的三个衔接子。如此产生的文库具有更高的表达开放阅读框的复杂性，因此可以提高功能蛋白质屏幕的成功，例如两个混合屏幕。适配器还具有用于系统之间有效克隆和转移的重组位点。

5. 发明授权

US06916423B2 Device and methods for subdividing and filtering gel material and extracting molecules therefrom 失效
标题翻译：用于细分和过滤凝胶材料并从中提取分子的装置和方法
公开(公告)号：US06916423B2
公开(公告)日：2005-07-12
申请号：US09906792
申请日：2001-07-18
申请人： Roumen A. Bogoev , Scott E. Whitney , Joseph W. Amshey
发明人： Roumen A. Bogoev , Scott E. Whitney , Joseph W. Amshey
IPC分类号： G01N33/48 , C07K1/34 , C12M1/00 , C12M1/33 , C12N15/09 , C12Q1/68 , G01N1/10 , G01N1/28 , B01D11/04 , G01N27/26
CPC分类号： C07K1/34 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： An apparatus and method for mincing a gel includes a gel mincing tube and a mesh material. The mesh material extends across the end of the tube. To subdivide a gel using the mincing apparatus, a gel is placed upon the mesh material in the mincing tube, the mincing tube, mesh material and the gel are spun in a centrifuge, forcing the gel through the mesh material so that the gel is subdivided into generally uniform smaller fragments. The mesh material may be secured to a tube in the form of a nesting tube. The nesting tube nests within the opening of a recovery vessel. The mesh material may be placed in series with a conditionally porous membrane in the nesting tube. Centrifuging the nesting tube and the recovery vessel subdivides gel material into fragments by forcing the gel through the mesh material. The gel subsequently falls upon the membrane, and may be treated on the membrane to extract or otherwise treat analytes in the gel material.
摘要翻译：用于切割凝胶的设备和方法包括凝胶切割管和网状材料。网状材料延伸穿过管的端部。为了使用切碎装置对凝胶进行细分，将凝胶放置在打孔管中的网状材料上，在离心机中将切细管，网状材料和凝胶纺丝，迫使凝胶通过网状材料，使凝胶细分变成大致均匀的较小碎片。网状材料可以以嵌套管的形式固定到管。嵌套管嵌套在回收容器的开口内。网状材料可以与嵌套管中的条状多孔膜串联放置。将嵌套管和回收容器离心通过使凝胶通过网状材料将凝胶材料细分成碎片。凝胶随后落在膜上，并且可以在膜上处理以提取或以其它方式处理凝胶材料中的分析物。

6. 发明申请

US20100059380A1 APPARATUS FOR CONCURRENT ELECTROPHORESIS IN A PLURALITY OF GELS 审中-公开
标题翻译：在多种凝胶中进行电流电泳的装置
公开(公告)号：US20100059380A1
公开(公告)日：2010-03-11
申请号：US12533907
申请日：2009-07-31
申请人： Thomas R. JACKSON , Adam S. Henry , Joseph W. Amshey , Roumen A. Bogoev
发明人： Thomas R. JACKSON , Adam S. Henry , Joseph W. Amshey , Roumen A. Bogoev
IPC分类号： G01N27/26
CPC分类号： G01N27/44704 , G01N27/44782
摘要： Apparatus and methods for conducting electrophoretic separation concurrently in a plurality of gels with improved reproducibility among the gels.
摘要翻译：在多个凝胶中同时进行电泳分离的装置和方法，其具有改进的凝胶重现性。

7. 发明申请

US20110098447A1 Homogenous Populations of Molecules 审中-公开
标题翻译：均质的分子群体
公开(公告)号：US20110098447A1
公开(公告)日：2011-04-28
申请号：US12861764
申请日：2010-08-23
申请人： Joseph W. AMSHEY , Roumen A. Bogoev
发明人： Joseph W. AMSHEY , Roumen A. Bogoev
IPC分类号： C07K14/00 , C07K1/107
CPC分类号： G01N33/6842 , C40B40/10 , G01N2496/00
摘要： The invention provides populations of molecules that are prepared as, or treated to become, homogeneous for one or more molecular characteristics. In an aspect, the invention relates to molecular weight standards that may be used to determine the molecular weight or apparent molecular weight of uncharacterized molecules, such as proteins and nucleic acids, as well as in other applications. In one aspect, the molecular weight standards are pre-stained.
摘要翻译：本发明提供了为一种或多种分子特征制备或被处理成均匀的分子群体。在一方面，本发明涉及可用于测定未表征的分子（例如蛋白质和核酸）以及其它应用的分子量或表观分子量的分子量标准品。在一个方面，分子量标准物被预染色。

8. 发明授权

US07781173B2 Homogeneous populations of molecules 有权
标题翻译：均质群体的分子
公开(公告)号：US07781173B2
公开(公告)日：2010-08-24
申请号：US10949471
申请日：2004-09-24
申请人： Joseph W. Amshey , Roumen A. Bogoev
发明人： Joseph W. Amshey , Roumen A. Bogoev
IPC分类号： G01N33/53 , C12Q1/68
CPC分类号： G01N33/6842 , C40B40/10 , G01N2496/00
摘要： The invention provides populations of molecules that are prepared as, or treated to become, homogeneous for one or more molecular characteristics. In an aspect, the invention relates to molecular weight standards that may be used to determine the molecular weight or apparent molecular weight of uncharacterized molecules, such as proteins and nucleic acids, as well as in other applications. In one aspect, the molecular weight standards are pre-stained.
摘要翻译：本发明提供了为一种或多种分子特征制备或被处理成均匀的分子群体。在一方面，本发明涉及可用于测定未表征的分子（例如蛋白质和核酸）以及其它应用的分子量或表观分子量的分子量标准品。在一个方面，分子量标准物被预染色。

9. 发明授权

US06758974B2 Device and methods for subdividing and filtering gel material and extracting molecules therefrom 失效
标题翻译：用于细分和过滤凝胶材料并从中提取分子的装置和方法
公开(公告)号：US06758974B2
公开(公告)日：2004-07-06
申请号：US09906792
申请日：2001-07-18
申请人： Roumen A. Bogoev , Scott E. Whitney , Joseph W. Amshey
发明人： Roumen A. Bogoev , Scott E. Whitney , Joseph W. Amshey
IPC分类号： B01D1104
CPC分类号： C07K1/34 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： An apparatus and method for mincing a gel includes a gel mincing tube and a mesh material. The mesh material extends across the end of the tube. To subdivide a gel using the mincing apparatus, a gel is placed upon the mesh material in the mincing tube, the mincing tube, mesh material and the gel are spun in a centrifuge, forcing the gel through the mesh material so that the gel is subdivided into generally uniform smaller fragments. The mesh material may be secured to a tube in the form of a nesting tube. The nesting tube nests within the opening of a recovery vessel. The mesh material may be placed in series with a conditionally porous membrane in the nesting tube. Centrifuging the nesting tube and the recovery vessel subdivides gel material into fragments by forcing the gel through the mesh material. The gel subsequently falls upon the membrane, and may be treated on the membrane to extract or otherwise treat analytes in the gel material.
摘要翻译：用于切割凝胶的设备和方法包括凝胶切割管和网状材料。网状材料延伸穿过管的端部。为了使用切碎装置对凝胶进行细分，将凝胶放置在打孔管中的网状材料上，在离心机中将切细管，网状材料和凝胶纺丝，迫使凝胶通过网状材料，使凝胶细分变成大致均匀的较小碎片。网状材料可以以嵌套管的形式固定到管。嵌套管嵌套在回收容器的开口内。网状材料可以与嵌套管中的条状多孔膜串联放置。将嵌套管和回收容器离心通过使凝胶通过网状材料将凝胶材料细分成碎片。凝胶随后落在膜上，并且可以在膜上处理以提取或以其它方式处理凝胶材料中的分析物。

10. 发明申请

US20090087873A1 SHARPLY RESOLVING LABELED PROTEIN MOLECULAR WEIGHT STANDARDS 审中-公开
标题翻译：夏普解析标签蛋白分子量标准
公开(公告)号：US20090087873A1
公开(公告)日：2009-04-02
申请号：US11781251
申请日：2007-07-21
申请人： Roumen A. Bogoev , Douglas Kang
发明人： Roumen A. Bogoev , Douglas Kang
IPC分类号： C12Q1/32 , G01N31/00 , C12Q1/26
CPC分类号： G01N27/4163 , C07K1/126 , C07K1/13 , C07K1/26 , Y10T436/105831
摘要： Pre-labeled protein standards useful in electrophoresis that have sharp, consistent separation characteristics that are substantially the same as those of their unlabeled counterparts are provided. The invention provides pre-labeled protein standard sets that include a plurality of labeled proteins that are labeled on a first amino acid, in which side reactions of the label with amino acids not targeted for labeling are reduced.
摘要翻译：提供了具有与其未标记对应物基本上相同的清晰，一致的分离特性的电泳中有用的预标记的蛋白质标准品。本发明提供了预标记的蛋白质标准组，其包括在第一氨基酸上标记的多个标记的蛋白质，其中标记物与未标记靶标的氨基酸的副反应降低。

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