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1. 发明授权

US07238528B2 Means and methods for fibroblast-like or macrophage-like cell transduction 有权
标题翻译：成纤维细胞样或巨噬细胞样细胞转导的方法和方法
公开(公告)号：US07238528B2
公开(公告)日：2007-07-03
申请号：US10783510
申请日：2004-02-20
申请人： Ronald Vogels , Govert Johan Schouten , Abraham Bout , Menzo Jans Emco Havenga
发明人： Ronald Vogels , Govert Johan Schouten , Abraham Bout , Menzo Jans Emco Havenga
IPC分类号： C12N15/00
CPC分类号： C12N15/86 , A61K38/45 , A61K48/00 , C12N2710/10343 , C12N2710/10345 , C12N2810/6018
摘要： The invention provides a nucleic acid delivery vehicle with or having been provided with at least a tissue tropism for fibroblast-like or macrophage-like cells, preferably synoviocytes. In one aspect, the nucleic acid delivery vehicle is a virus capsid or a functional part, derivative and/or analogue thereof. Preferably, the virus capsid is an adenovirus capsid. Preferably, the adenovirus is a subgroup B adenovirus, such as adenovirus 16. Preferably, the tissue tropism is provided by at least a tissue tropism determining part of an adenovirus fiber protein or a functional derivative and/or analogue thereof. The invention further presents methods for the treatment of diseases, such as joint related diseases.
摘要翻译：本发明提供具有或已经提供至少一种成纤维细胞样细胞或巨噬细胞样细胞的组织嗜性的核酸递送载体，优选滑膜细胞。一方面，核酸递送载体是病毒衣壳或其功能部分，衍生物和/或类似物。优选地，病毒衣壳是腺病毒衣壳。优选地，腺病毒是亚类B腺病毒，例如腺病毒16.优选地，组织向性由至少组织嗜性确定部分腺病毒纤维蛋白或其功能衍生物和/或类似物提供。本发明还提供了治疗诸如关节疾病的疾病的方法。

2. 发明授权

US07491532B2 Recombinant protein production in a human cell 有权
标题翻译：在人细胞中重组蛋白质的产生
公开(公告)号：US07491532B2
公开(公告)日：2009-02-17
申请号：US10790562
申请日：2004-03-01
申请人： Abraham Bout , Guus Hateboer , Karina Cornelia Verhulst , Alphonsus Gerardus Uytdehaag , Govert Johan Schouten
发明人： Abraham Bout , Guus Hateboer , Karina Cornelia Verhulst , Alphonsus Gerardus Uytdehaag , Govert Johan Schouten
IPC分类号： C12N15/85 , C12N15/00 , C12N15/09 , C12N15/63
CPC分类号： C12N15/86 , C07K14/005 , C12N2710/10322 , C12N2710/10332 , C12N2710/10343 , C12N2740/16122 , C12N2740/16234 , C12N2760/16122 , C12N2760/16134 , C12N2760/16151 , C12N2800/108 , C12N2830/00 , C12N2830/15 , C12N2830/60
摘要： Methods and compositions for the production of recombinant proteins in a eukaryotic cell line are disclosed. The methods and compositions are particularly useful for generating stable expression of recombinant proteins of interest that are modified post-translationally, for example, by glycosylation. Such proteins may have advantageous properties in comparison with their counterparts produced in non-human systems such as Chinese hamster ovary cells.
摘要翻译：公开了用于在真核细胞系中生产重组蛋白的方法和组合物。所述方法和组合物特别可用于产生翻译后修饰的重组蛋白质的稳定表达，例如通过糖基化。与在非人系统如中国仓鼠卵巢细胞中产生的对应物相比，这样的蛋白质可能具有有利的性质。

3. 发明申请

US20080274497A1 RECOMBINANT PROTEIN PRODUCTION IN A HUMAN CELL 有权
标题翻译：人类细胞中重组蛋白的生产
公开(公告)号：US20080274497A1
公开(公告)日：2008-11-06
申请号：US10790562
申请日：2004-03-01
申请人： Abraham Bout , Guus Hateboer , Karina Cornelia Verhulst , Alphonsus Gerardus Uytdehaag , Govert Johan Schouten
发明人： Abraham Bout , Guus Hateboer , Karina Cornelia Verhulst , Alphonsus Gerardus Uytdehaag , Govert Johan Schouten
IPC分类号： C12P21/06 , C12N5/08 , C12N15/861
CPC分类号： C12N15/86 , C07K14/005 , C12N2710/10322 , C12N2710/10332 , C12N2710/10343 , C12N2740/16122 , C12N2740/16234 , C12N2760/16122 , C12N2760/16134 , C12N2760/16151 , C12N2800/108 , C12N2830/00 , C12N2830/15 , C12N2830/60
摘要： Methods and compositions for the production of recombinant proteins in a eukaryotic cell line are disclosed. The methods and compositions are particularly useful for generating stable expression of recombinant proteins of interest that are modified post-translationally, for example, by glycosylation. Such proteins may have advantageous properties in comparison with their counterparts produced in non-human systems such as Chinese hamster ovary cells.
摘要翻译：公开了用于在真核细胞系中生产重组蛋白的方法和组合物。所述方法和组合物特别可用于产生翻译后修饰的重组蛋白质的稳定表达，例如通过糖基化。与在非人系统如中国仓鼠卵巢细胞中产生的对应物相比，这样的蛋白质可能具有有利的性质。

4. 发明授权

US07132280B2 Recombinant protein production in a human cell 有权
公开(公告)号：US07132280B2
公开(公告)日：2006-11-07
申请号：US10234007
申请日：2002-09-03
申请人： Abraham Bout , Guus Hateboer , Karina Cornelia Verhulst , Alphonsus Gerardus Uytdehaag , Govert Johan Schouten
发明人： Abraham Bout , Guus Hateboer , Karina Cornelia Verhulst , Alphonsus Gerardus Uytdehaag , Govert Johan Schouten
IPC分类号： C12N5/08 , A61K39/395
CPC分类号： C12N15/86 , C07K14/005 , C12N2710/10322 , C12N2710/10332 , C12N2710/10343 , C12N2740/16122 , C12N2740/16234 , C12N2760/16122 , C12N2760/16134 , C12N2760/16151 , C12N2800/108 , C12N2830/00 , C12N2830/15 , C12N2830/60
摘要： Methods and compositions for the production of recombinant proteins in a human cell line. The methods and compositions are particularly useful for generating stable expression of human recombinant proteins of interest that are modified post-translationally, for example, by glycosylation. Such proteins may have advantageous properties in comparison with their counterparts produced in non-human systems such as Chinese hamster ovary cells.

5. 发明授权

US06855544B1 Recombinant protein production in a human cell 有权
标题翻译：在人细胞中重组蛋白质的产生
公开(公告)号：US06855544B1
公开(公告)日：2005-02-15
申请号：US09549463
申请日：2000-04-14
申请人： Guus Hateboer , Karina Cornelia Verhulst , Govert Johan Schouten , Alphonsus Gerardus Uytdehaag , Abraham Bout
发明人： Guus Hateboer , Karina Cornelia Verhulst , Govert Johan Schouten , Alphonsus Gerardus Uytdehaag , Abraham Bout
IPC分类号： C07K14/075 , C07K14/11 , C07K14/16 , C12N15/861 , C12N5/00 , C12N15/63 , C12P21/06 , C12N21/04
CPC分类号： C12N15/86 , C07K14/005 , C12N2710/10322 , C12N2710/10332 , C12N2710/10343 , C12N2740/16122 , C12N2740/16234 , C12N2760/16122 , C12N2760/16134 , C12N2760/16151 , C12N2800/108 , C12N2830/00 , C12N2830/15 , C12N2830/60
摘要： Methods and compositions for the production of recombinant proteins in a human cell line. The methods and positions are particularly useful for generating stable expression of human recombinant proteins of interest that are modified post-translationally, for example, by glycosylation. Such proteins may have advantageous properties in comparison with their counterparts produced in non-human systems such as Chinese Hamster Ovary cells.
摘要翻译：用于在人细胞系中产生重组蛋白的方法和组合物。方法和位置对于产生经翻译后修饰的人重组蛋白质的稳定表达特别有用，例如通过糖基化。与非人系统如中国仓鼠卵巢细胞中产生的蛋白质相比，这些蛋白质可能具有有利的性质。

6. 发明授权

US07527961B2 Production of vaccines 有权
标题翻译：生产疫苗
公开(公告)号：US07527961B2
公开(公告)日：2009-05-05
申请号：US11271368
申请日：2005-11-11
申请人： Maria Grazia Pau , Alphonsus G. C. M. UytdeHaag , Govert Johan Schouten
发明人： Maria Grazia Pau , Alphonsus G. C. M. UytdeHaag , Govert Johan Schouten
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , A61K2039/5252 , A61K2039/70 , C12N2720/12351 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2770/20051 , C12N2770/24051 , C12N2770/24151
摘要： Means and methods for producing mammalian viruses, the method comprising infecting a culture of immortalized human cells with a virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The viruses can be harvested and be used for the production of vaccines. Advantages include that human cells of the present invention can be cultured under defined serum-free conditions and the cells show improved capability for propagating virus. Methods are provided for producing, in cultured human cells, influenza virus and vaccines derived thereof. This method eliminates the necessity of using whole chicken embryos for the production of Influenza vaccines. The method also provides for the continuous or batch-wise removal of culture media. As such, the present invention allows the large-scale continuous production of viruses to a high titer.
摘要翻译：用于生产哺乳动物病毒的方法和方法，所述方法包括用病毒感染永生化人细胞的培养物，在病毒生长的条件下孵育感染病毒的培养物繁殖病毒，并形成含病毒的培养基，并除去含病毒的培养基。可以收获病毒并用于生产疫苗。优点包括本发明的人细胞可以在规定的无血清条件下进行培养，并且细胞显示出增强的病毒传播能力。提供了用于在培养的人类细胞中产生流感病毒及其衍生的疫苗的方法。该方法消除了使用全鸡胚胎生产流感疫苗的必要性。该方法还提供了连续或间歇地除去培养基。因此，本发明允许病毒的大规模连续生产达到高滴度。

7. 发明申请

US20090017068A1 Vaccines against West Nile Virus 审中-公开
标题翻译：针对西尼罗病毒的疫苗
公开(公告)号：US20090017068A1
公开(公告)日：2009-01-15
申请号：US12220971
申请日：2008-07-29
申请人： Alphonsus G.C.M. UytdeHaag , Govert Johan Schouten , Jaap Goudsmit
发明人： Alphonsus G.C.M. UytdeHaag , Govert Johan Schouten , Jaap Goudsmit
IPC分类号： A61K39/12 , C12N7/04 , C12N5/08 , A61P31/12
CPC分类号： A61K39/12 , A61K2039/5252 , A61K2039/552 , A61K2039/55505 , A61K2039/55566 , C12N7/00 , C12N2770/24134 , C12N2770/24163
摘要： Described are vaccines containing (whole-inactivated) West Nile Viruses and/or West Nile viral proteins derived therefrom, produced on human cells, wherein the human cells comprise a sequence encoding at least one early region-1 (E1) gene product of an adenovirus. The cells are preferably cultured in suspension to very high densities and under serum-free conditions. Herein, it is disclosed that use of such cells results in high titers of West Nile Virus produced.
摘要翻译：描述了在人细胞上产生的含有（全失活的）西尼罗病毒和/或由其衍生的西尼罗病毒蛋白质的疫苗，其中人细胞包含编码腺病毒的至少一个早期区域-1（E1）基因产物的序列。细胞优选在悬浮液中以非常高的密度和无血清条件培养。在本文中，公开了使用这种细胞导致西尼罗病毒产生的高滴度。

8. 发明授权

US07163787B2 Methods for the identification of antiviral compounds 有权
标题翻译：鉴定抗病毒化合物的方法
公开(公告)号：US07163787B2
公开(公告)日：2007-01-16
申请号：US10698086
申请日：2003-10-30
申请人： Ronald H. P. Brus , Govert Johan Schouten , Alphonsus G. C. M. UytdeHaag
发明人： Ronald H. P. Brus , Govert Johan Schouten , Alphonsus G. C. M. UytdeHaag
IPC分类号： C12Q1/00 , C12Q1/02 , C12Q1/18 , C12Q1/70 , C12N5/00 , C12N5/08 , C12N5/10
CPC分类号： G01N33/502 , G01N33/5008 , G01N33/5044 , G01N2333/075 , G01N2500/10
摘要： The present invention provides novel methods for determining whether a compound influences a phase in the life cycle of a virus comprising providing a cell with the compound and with at least a fragment of the virus sufficient for performing the phase and determining whether the phase is influenced in the cell, the cell comprising a nucleic acid encoding an adenovirus early protein or a functional part, derivative and/or analogue of the adenovirus early protein. In another aspect, the invention provides the use of a cell, the cell comprising nucleic acid encoding an adenovirus early protein, for screening a library of compounds for the presence of a compound capable of influencing a phase in the life cycle of a virus capable of entering the cell. The invention also provides novel methods for identifying a compound with antiviral activity comprising providing a cell with at least a fragment of a virus, the fragment capable of performing a step in the life cycle of the virus, providing the cell with a compound and determining whether the compound is capable of influencing the step in the life cycle of the virus, wherein the cell comprises a nucleic acid encoding an adenovirus early protein or a functional part, derivative and/or analogue of the adenovirus early protein.
摘要翻译：本发明提供了用于确定化合物是否影响病毒生命周期中的相的新方法，包括向细胞提供化合物和至少一个病毒片段足以进行相位并确定该相是否受到影响该细胞包含编码腺病毒早期蛋白的核酸或腺病毒早期蛋白的功能部分，衍生物和/或类似物。在另一方面，本发明提供了细胞的使用，所述细胞包含编码腺病毒早期蛋白的核酸，用于筛选化合物文库，所述化合物文库能够影响能够影响病毒的病毒的生命周期中的相进入细胞本发明还提供用于鉴定具有抗病毒活性的化合物的新方法，包括向细胞提供至少一种病毒片段，所述片段能够在病毒的生命周期中进行步骤，向细胞提供化合物并确定是否该化合物能够影响病毒生命周期中的步骤，其中细胞包含编码腺病毒早期蛋白的核酸或腺病毒早期蛋白的功能部分，衍生物和/或类似物。

9. 发明申请

US20120219586A1 ADJUVANTED VACCINE FORMULATIONS 有权
标题翻译： ADJUVANTED VACCINE配方
公开(公告)号：US20120219586A1
公开(公告)日：2012-08-30
申请号：US13499488
申请日：2010-10-01
申请人： Govert Johan Schouten , Cornelis Johannes Leenhouts
发明人： Govert Johan Schouten , Cornelis Johannes Leenhouts
IPC分类号： A61K39/145 , A61P31/04 , A61P31/16 , A61K39/09 , A61P37/04
CPC分类号： A61K39/292 , A61K35/74 , A61K39/00 , A61K39/12 , A61K39/145 , A61K39/39 , A61K2039/543 , A61K2039/545 , A61K2039/5555 , A61K2039/55594 , C12N2730/10134 , C12N2760/16134 , A61K2300/00 , A61K35/44
摘要： The invention relates to adjuvanted vaccine formulations, in particular influenza vaccines for intranasal delivery. Provided is an adjuvanted influenza vaccine formulation, comprising (i) peptidoglycan microparticles obtained from a Gram-positive bacterium and (ii) at least one influenza virus antigen or antigenic preparation thereof, which antigen or antigenic preparation is not fused or otherwise covalently attached to a proteinaceous peptidoglycan binding moiety.
摘要翻译：本发明涉及佐剂化疫苗制剂，特别是用于鼻内递送的流感疫苗。提供了一种佐剂化的流感疫苗制剂，其包含（i）从革兰氏阳性细菌获得的肽聚糖微粒和（ii）至少一种流感病毒抗原或其抗原制剂，所述抗原或抗原制剂不融合或以其他方式共价连接到蛋白质肽聚糖结合部分。

10. 发明授权

US09878034B2 Adjuvanted vaccine formulations 有权
公开(公告)号：US09878034B2
公开(公告)日：2018-01-30
申请号：US13499488
申请日：2010-10-01
申请人： Govert Johan Schouten , Cornelis Johannes Leenhouts
发明人： Govert Johan Schouten , Cornelis Johannes Leenhouts
IPC分类号： A61K39/145 , A61P31/04 , A61K39/29 , A61K35/74 , A61K39/39 , A61K39/12 , A61K39/00
CPC分类号： A61K39/292 , A61K35/74 , A61K39/00 , A61K39/12 , A61K39/145 , A61K39/39 , A61K2039/543 , A61K2039/545 , A61K2039/5555 , A61K2039/55594 , C12N2730/10134 , C12N2760/16134 , A61K2300/00 , A61K35/44
摘要： The invention relates to adjuvanted vaccine formulations, in particular influenza vaccines for intranasal delivery. Provided is an adjuvanted influenza vaccine formulation, comprising (i) peptidoglycan microparticles obtained from a Gram-positive bacterium and (ii) at least one influenza virus antigen or antigenic preparation thereof, which antigen or antigenic preparation is not fused or otherwise covalently attached to a proteinaceous peptidoglycan binding moiety.

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