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1. 发明授权

US4381345A Pretreatment of glucose feedstock with reducing agents 失效
标题翻译：用还原剂预处理葡萄糖原料
公开(公告)号：US4381345A
公开(公告)日：1983-04-26
申请号：US261996
申请日：1981-05-08
申请人： Ronald P. Rohrbach , Mary J. Maliarik
发明人： Ronald P. Rohrbach , Mary J. Maliarik
IPC分类号： C12P19/24 , C12P7/00
CPC分类号： C12P19/24
摘要： Commercial glucose solutions used as feedstocks for enzymatic conversion of glucose to fructose by glucose isomerase often contain materials which act as poisons toward the enzyme. It has been found that these poisons can be removed, or destroyed, by treatment of the feedstock with reducing agents which are water soluble and water stable metal hydrides, such as sodium borohydride.
摘要翻译：用作葡萄糖异构酶将葡萄糖酶促转化为果糖的原料的商业葡萄糖溶液通常含有作为对该酶的毒物的物质。已经发现，通过用水溶性和水稳定的金属氢化物如硼氢化钠的还原剂处理原料，可以除去或破坏这些毒素。

2. 发明授权

US4511654A Production of high sugar syrups 失效
标题翻译：生产高糖糖浆
公开(公告)号：US4511654A
公开(公告)日：1985-04-16
申请号：US499652
申请日：1983-05-31
申请人： Ronald P. Rohrbach , Mary J. Maliarik , Thomas P. Malloy
发明人： Ronald P. Rohrbach , Mary J. Maliarik , Thomas P. Malloy
IPC分类号： C12P19/20 , C12P19/22 , C12N11/14
CPC分类号： C12P19/22 , C12P19/20
摘要： The preparation of syrups which contain a high glucose or maltose content may be effected in a two-step process. In the first step, a feedstock such as starch which has been pretreated with alpha-amylase to adjust the dextrose equivalent is contacted with an enzyme such as amyloglucosidase or beta-amylase immobilized on a solid support. The contact time is adjusted by correlating the residence time and the liquid hourly space velocity so as to provide a conversion percentage of starch in the range of from about 50% to 85%, thus avoiding the formation of undesired conversion products. The partially hydrolyzed reaction mixture from the enzyme treatment is then passed through an ultrafiltration membrane whereby the permeate comprising the high glucose or maltose content is recovered, while the retentate is recycled for admixture with the partially hydrolyzed reaction mixture or for further treatment with the immobilized enzyme.
摘要翻译：含有高葡萄糖或麦芽糖含量的糖浆的制备可以两步法进行。在第一步中，将已经用α-淀粉酶预处理以调节葡萄糖当量的原料如淀粉与固定在固体支持物上的酶如淀粉葡糖苷酶或β-淀粉酶接触。通过将停留时间和液体时空速相关联来调节接触时间，从而提供约50％至85％范围内淀粉的转化率，从而避免形成不期望的转化产物。然后将来自酶处理的部分水解的反应混合物通过超滤膜，由此回收包含高葡萄糖或麦芽糖含量的渗透物，同时将渗余物再循环用于与部分水解的反应混合物混合或用固定化酶进一步处理。

3. 发明授权

US4415656A Increasing the stability of amyloglucosidase 失效
标题翻译：提高淀粉葡萄糖苷酶的稳定性
公开(公告)号：US4415656A
公开(公告)日：1983-11-15
申请号：US380797
申请日：1982-05-21
申请人： Ronald P. Rohrbach , Mary J. Maliarik
发明人： Ronald P. Rohrbach , Mary J. Maliarik
IPC分类号： C12N9/00 , C12N9/34 , C12P19/20
CPC分类号： C12N9/2428 , C12N9/00 , C12P19/20
摘要： Thermal deactivation of amyloglucosidase is substantially retarded at elevated pressures relative to atmospheric pressure. The productivity of glucose formation from amyloglucosidase-catalyzed hydrolysis of starch or partially hydrolyzed starch is significantly increased when the hydrolysis is performed at a pressure greater than about 500 psig.
摘要翻译：淀粉葡糖苷酶的热失活在相对于大气压的升高的压力下基本上延迟。当在大约500psig的压力下进行水解时，由淀粉或部分水解淀粉的淀粉葡糖苷酶催化的水解产生葡萄糖的生产率显着增加。

4. 发明授权

US4382121A Pretreatment of glucose feedstock 失效
标题翻译：葡萄糖原料的预处理
公开(公告)号：US4382121A
公开(公告)日：1983-05-03
申请号：US261995
申请日：1981-05-08
申请人： Ronald P. Rohrbach , Mary J. Maliarik
发明人： Ronald P. Rohrbach , Mary J. Maliarik
IPC分类号： C12P19/24 , C12P7/00
CPC分类号： C12P19/24
摘要： Commercial glucose solutions used as feedstock for enzymatic conversion of glucose to fructose by glucose isomerase often contain materials which act as poisons toward the enzyme. It has been found that these poisons can be removed, or destroyed, by treatment of the feedstock with oxidizing agents, chief of which is hydrogen peroxide.
摘要翻译：用作葡萄糖异构酶将葡萄糖酶转化为果糖的原料的商业葡萄糖溶液通常含有作为对该酶的毒物的物质。已经发现，通过用主要是过氧化氢的氧化剂处理原料，可以除去或破坏这些毒素。

5. 发明授权

US4292199A Method of preparing a support matrix 失效
标题翻译：制备支撑体的方法
公开(公告)号：US4292199A
公开(公告)日：1981-09-29
申请号：US178659
申请日：1980-08-18
申请人： Ronald P. Rohrbach , Mary J. Maliarik
发明人： Ronald P. Rohrbach , Mary J. Maliarik
IPC分类号： B01J31/00 , B01J31/02 , B01J31/06 , C12N11/08 , C12N11/14
CPC分类号： B01J31/0254 , B01J31/003 , B01J31/06 , C12N11/08 , C12N11/14
摘要： An improved method of preparing support matrices, characterized as inorganic oxides impregnated with a polyamine cross-linked with a bifunctional reagent so as to furnish a plurality of pendant functional groups, comprises impregnating the oxide at a pH less than about 4 and subsequently curing the polyamine-impregnated oxide at a pH from about 8 to about 12. This improved method leads to subsequently prepared immobilized enzyme systems whose activity is increased by about 35%.
摘要翻译：一种改进的制备支撑基质的方法，其特征在于用双官能试剂交联的多胺浸渍的无机氧化物，以便提供多个侧链官能团，包括在小于约4的pH下浸渍氧化物，随后固化多胺在约8至约12的pH下的浸渍氧化物。该改进的方法导致随后制备的活性增加约35％的固定化酶体系。

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