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1. 发明授权

US5439797A Detection of analytes using fluorescent energy transfer 失效
标题翻译：使用荧光能量转移检测分析物
公开(公告)号：US5439797A
公开(公告)日：1995-08-08
申请号：US114103
申请日：1993-08-30
申请人： Roger Y. Tsien , Susan S. Taylor , Stephen R. Adams , Ying Ji
发明人： Roger Y. Tsien , Susan S. Taylor , Stephen R. Adams , Ying Ji
IPC分类号： C12N9/00 , C12Q1/48 , G01N21/64 , G01N33/542 , G01N33/58 , G01N33/68 , G01N33/533
CPC分类号： G01N33/542 , G01N33/582 , Y10S436/80
摘要： The invention provides labeled proteins suitable for determining the presence of cAMP, other second messengers and organic molecules. The proteins are separately labeled with fluorochromes which, when in close spatial proximity, preferably, less than about 6 nm, interact through the transfer of energy from one fluorochrome to the other.A composition of matter, (S.sub.1.A).sub.n1 (S.sub.2.D).sub.n2 is provided wherein S.sub.1 and S.sub.2 are two proteins which are associated in one state and substantially disassociated in another, the equilibrium between which is controlled by the free concentration of an analyte, and A and D are fluorochromes, the emission wavelength of fluorochrome D overlapping the excitation wavelength of fluorochrome A and the distance between A and D being in sufficiently close proximity to allow the radiationless transfer of energy between the fluorochromes. A and D can be selected from a variety of acceptor, donor pairs such as fluorescein and tetramethylrhodamine and derivatives thereof. The concentration of analytes such as cAMP in a sample can be determined by contacting the sample with (S.sub.1.A).sub.n1 (S.sub.2.D).sub.n2 providing energy near the excitation wavelength of D and measuring the fluorescence of A or D, the concentration of cAMP and other said analytes being determined by the ratio of emission of D to the emission of A, previously calibrated with reference solutions of known analyte concentration.
摘要翻译：本发明提供了适合于测定cAMP，其他第二信使和有机分子的存在的标记蛋白质。蛋白质分别用荧光染料标记，当空间接近时，优选小于约6nm，通过将能量从一个荧光染料转移到另一个荧光染料相互作用。提供了一种物质组合物（S1.A）n1（S2.D）n2，其中S1和S2是两种蛋白质，两种蛋白质在一种状态下相互关联，并且在另一种状态下基本上脱离，其中的平衡受自由浓度分析物，A和D是荧光染料，荧光染料D的发射波长与荧光染料A的激发波长重叠，A和D之间的距离足够接近，从而允许在荧光染料之间无辐射传递能量。 A和D可以选自多种受体，诸如荧光素和四甲基罗丹明的供体对及其衍生物。通过使样品与（S1.A）n1（S2.D）n2接触，在D的激发波长附近提供能量并测量A或D的荧光，可以测定样品中cAMP等分析物的浓度， cAMP和其他所述分析物由D的排放与A的排放比例决定，其先前用已知分析物浓度的参考溶液校准。

2. 发明申请

US20090061442A1 Screening assay to identify non-ATP-competitors targeting protein kinase A 有权
标题翻译：筛选测定以鉴定靶向蛋白激酶A的非ATP竞争物
公开(公告)号：US20090061442A1
公开(公告)日：2009-03-05
申请号：US12151318
申请日：2008-05-05
申请人： Susan S. Taylor , Sanjay A. Saldanha
发明人： Susan S. Taylor , Sanjay A. Saldanha
IPC分类号： C12Q1/68
CPC分类号： G01N33/542 , C12Q1/485
摘要： Methods for screening compounds for their activity as a protein kinase A modulators are provided. The methods are based on fluorescence polarization of peptide probes to identify drug candidates that act by activating or inhibiting the catalytic function of PKA. In certain embodiments, the methods are adapted for high throughput screening.
摘要翻译：提供了筛选化合物作为蛋白激酶A调节剂的活性的方法。该方法基于肽探针的荧光偏振，以鉴定通过激活或抑制PKA的催化功能起作用的候选药物。在某些实施方案中，该方法适用于高通量筛选。

3. 发明授权

US07892776B2 Screening assay to identify modulators of protein kinase A 有权
标题翻译：筛选测定蛋白激酶A的调节剂
公开(公告)号：US07892776B2
公开(公告)日：2011-02-22
申请号：US12151318
申请日：2008-05-05
申请人： Susan S. Taylor , Sanjay A. Saldanha
发明人： Susan S. Taylor , Sanjay A. Saldanha
IPC分类号： C12Q1/46
CPC分类号： G01N33/542 , C12Q1/485
摘要： Methods for screening compounds for their activity as a protein kinase A modulators are provided. The methods are based on fluorescence polarization of peptide probes to identify drug candidates that act by activating or inhibiting the catalytic function of PKA. In certain embodiments, the methods are adapted for high throughput screening.
摘要翻译：提供了筛选化合物作为蛋白激酶A调节剂的活性的方法。该方法基于肽探针的荧光偏振，以鉴定通过激活或抑制PKA的催化功能起作用的候选药物。在某些实施方案中，该方法适用于高通量筛选。

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