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1. 发明授权

US5352668A Product and method for improving keratinocyte adhesion to the dermis 失效
标题翻译：改善角质形成细胞对真皮粘附的产物和方法
公开(公告)号：US5352668A
公开(公告)日：1994-10-04
申请号：US936850
申请日：1992-08-28
申请人： Robnert E. Burgeson , Gregory P. Lunstrum , Patricia Rousselle , Douglas R. Keene , M. Peter Marinkovich
发明人： Robnert E. Burgeson , Gregory P. Lunstrum , Patricia Rousselle , Douglas R. Keene , M. Peter Marinkovich
IPC分类号： A61K38/17 , A61K35/12 , A61K35/36 , A61K38/39 , A61L27/22 , A61L27/60 , A61P17/00 , C07K1/22 , C07K14/47 , C07K14/78 , C07K16/00 , C07K16/18 , C12N5/00 , C12N5/071 , A61F2/10 , A61K37/12 , C07K15/06 , C07K15/20
CPC分类号： C12N5/0629 , A61K35/36 , A61K38/39 , A61L27/227 , A61L27/60 , C07K14/47 , C07K14/78 , C07K16/18 , C12N5/0068 , A61K35/12 , C12N2501/58 , C12N2533/50 , C12N2533/52 , Y10S530/842 , Y10S530/851 , Y10S623/915
摘要： A purified protein kalinin is disclosed that provides adhesion between epidermal keratinocytes and the underlying dermis. Purified kalinin localizes to the anchoring filaments of basement membranes or human subepithelial skin, trachea, esophagus, cornea and amnion when such areas are probed with BM165 monoclonal antibody after localization. The protein has a molecular weight of approximately 400-460 kDa and exists in a cell-associated form (about 460 kDa) and two medium-associated forms (about 440 and 400 kDa, respectively). The cell-associated form comprises a 200-, a 155- and a 140-kDa subunit, all normally held together by disulflde bonds. The cell-associated form is subjected to extracellular processing to produce the two medium-associated forms, wherein, in the 440-kDa form, the 200-kDa subunit has been processed to a 165-kDa subunit and, in the 400-kDa form, the 155-kDa subunit has been processed to a 105-KDa subunit. The BM165 epitope is located on the 165-kDa subunit. Kalinin has a rotary-shadow image revealing an asymmetric rod 107-nm long having two globules at a first end and a single globule at an opposing end. A method is also disclosed for improving adhesion of transplanted keratinocytes to an underlying substrate, such as the human dermis, by optimizing production of kalinin from cultured keratinocytes, or by providing an exogenous source of kalinin between the keratinocytes and substrate.
摘要翻译：公开了提供表皮角化细胞和潜在真皮之间的粘附的纯化的蛋白质卡林林。纯化的卡林林定位于基底膜或人类上皮皮肤，气管，食道，角膜和羊膜的锚定细丝上，当这些区域在定位后用BM165单克隆抗体探测时。该蛋白质具有约400-460kDa的分子量，并以细胞相关形式（约460kDa）和两种培养基相关形式（分别为约440和400kDa）存在。细胞相关形式包括通常由二硫键连接在一起的200个，一个155和一个140kDa的亚基。将细胞相关形式进行细胞外加工以产生两种中等相关形式，其中在440-kDa形式中，200kDa亚基已经被加工成165-kDa亚基，并且以400-kDa形式，155-kDa亚基已被处理成105-KDa亚基。 BM165表位位于165-kDa亚基上。加里宁具有旋转阴影图像，其显示107nm长的不对称杆，在第一端具有两个球，在相对端具有单个球。还公开了通过优化来自培养的角质形成细胞的生产kalinin或通过在角质形成细胞和底物之间提供外源的卡林林来改善移植的角质形成细胞与底物（例如人真皮）的粘附的方法。

2. 发明授权

US5770562A Products and methods for improving keratinocyte adhesion to the dermis 失效
标题翻译：改善角质细胞对真皮粘附的产品和方法
公开(公告)号：US5770562A
公开(公告)日：1998-06-23
申请号：US469500
申请日：1995-06-06
申请人： Robert E. Burgeson , Gregory P. Lunstrum , Patricia Rousselle , Douglas R. Keene , M. Peter Marinkovich
发明人： Robert E. Burgeson , Gregory P. Lunstrum , Patricia Rousselle , Douglas R. Keene , M. Peter Marinkovich
IPC分类号： A61K38/39 , A61L27/22 , A61L27/60 , C12N5/00 , C12N5/071 , C07K14/78
CPC分类号： C12N5/0629 , A61K38/39 , A61L27/227 , A61L27/60 , C12N5/0068 , C12N2501/58 , C12N2533/50 , C12N2533/52 , Y10S530/842
摘要： The isolated proteins kalinin and k-laminin are disclosed to provide adhesion between epidermal keratinocytes and the underlying dermis. Purified kalinin localizes to the anchoring filaments of basement membranes of human subepithelial skin, trachea, esophagus, cornea and amnion when such areas are probed with BM165 monoclonal antibody after localization. The protein has a molecular weight of approximately 410-495 kDa and exists in a cell-associated form (about 495 kDa) and two medium-associated forms (about 460 and 410 kDa, respectively). The BM165 epitope is located on the 165-kDa and 200 kDa subunits. Kalinin has a rotary-shadow image revealing an asymmetric rod 107-nm long having two globules at a first end and a single globule at an opposing end. The k-laminin adhesion molecule is an isolated heterotrimeric laminin variant that has a molecular weight of about 650 kDa and separates on western blots into first and second fragments that are similar to the B1 and B2 fragments of laminin. K-laminin also includes a third fragment of 190 kDa that is not immunoreactive with monoclonal antibodies 1F5, 11D5 and 4C7, but is immunoreactive with a monoclonal antibody BM165. Kalinin and k-laminin are preferably covalently associated. A method is also disclosed for improving adhesion of transplanted keratinocytes to an underlying substrate, such as the human dermis, by optimizing production of kalinin from cultured keratinocytes, or by providing an exogenous source of kalinin between the keratinocytes and substrate.
摘要翻译：公开了分离的蛋白质kalinin和k-层粘连蛋白以提供表皮角化细胞和潜在的真皮之间的粘附。纯化的卡林林定位于人类上皮皮肤，气管，食管，角膜和羊膜的基底膜的锚定细丝上，当这些区域在定位后用BM165单克隆抗体探测时。蛋白质具有约410-495kDa的分子量，并以细胞相关形式（约495kDa）和两种培养基相关形式（分别为约460和410kDa）存在。 BM165表位位于165kDa和200kDa亚基上。加里宁具有旋转阴影图像，其显示107nm长的不对称杆，在第一端具有两个球，在相对端具有单个球。 k-层粘连蛋白粘附分子是分离的异三聚体层粘连蛋白变体，其分子量为约650kDa，并将蛋白质印迹分离成与层粘连蛋白的B1和B2片段相似的第一和第二片段。 K-层粘连蛋白还包括与单克隆抗体1F5,11D5和4C7不具有免疫反应性的190kDa的第三片段，但是与单克隆抗体BM165具有免疫反应性。卡利宁和k-层粘连蛋白优选共价结合。还公开了通过优化来自培养的角质形成细胞的生产kalinin或通过在角质形成细胞和底物之间提供外源的卡林林来改善移植的角质形成细胞与底物（例如人真皮）的粘附的方法。

3. 发明授权

US6133236A Products and methods for improving keratinocyte adhesion to the dermis 失效
标题翻译：改善角质细胞对真皮粘附的产品和方法
公开(公告)号：US6133236A
公开(公告)日：2000-10-17
申请号：US815886
申请日：1997-03-12
申请人： Robert E. Burgeson , Gregory P. Lunstrum , Patricia Rouselle , Douglas R. Keene , M. Peter Marinkovich
发明人： Robert E. Burgeson , Gregory P. Lunstrum , Patricia Rouselle , Douglas R. Keene , M. Peter Marinkovich
IPC分类号： A61K38/39 , A61L24/10 , A61L27/22 , A61L27/60 , A61F2/10
CPC分类号： A61K38/39 , A61L24/108 , A61L27/227 , A61L27/60 , C12N2501/58 , C12N2533/50 , C12N2533/52
摘要： Improving adhesion of epidermis to an underlying dermal substrate including the step of providing an amount of purified kalinin between the epidermis and dermal substrate wherein the kalinin provides adhesion between the human dermis and epidermis wherein said kalinin is a trimeric molecule having the following three nonidentical chains: a chain which includes the BM165 epitope, either a 155 kilodalton chain or a 105 kilodalton chain, and a 140 kilodalton chain.
摘要翻译：改善表皮与潜在皮肤基质的粘附，包括在表皮和皮肤底物之间提供一定量的纯化的卡林林的步骤，其中所述卡林林提供人真皮和表皮之间的粘附，其中所述卡林林是具有以下三个不相同链的三聚体分子：包括BM165表位，155千道尔顿链或105千道尔顿链和140千道尔顿链的链。

4. 发明申请

US20080171044A1 COMPOSITIONS AND METHODS FOR INHIBITING SQUAMOUS CELL CARCINOMA 有权
标题翻译：用于抑制细胞癌的组合物和方法
公开(公告)号：US20080171044A1
公开(公告)日：2008-07-17
申请号：US11943486
申请日：2007-11-20
申请人： M. Peter Marinkovich
发明人： M. Peter Marinkovich
IPC分类号： A61K39/00 , G01N33/53
CPC分类号： G01N33/57484 , A61K2039/505 , C07K16/30 , G01N2333/78
摘要： The present invention relates to compositions and methods for detecting and inhibiting squamous cell carcinoma using agents that target the laminin 5 alpha 3 G4-G5 domain.
摘要翻译：本发明涉及使用靶向层粘连蛋白5α3G4-G5结构域的试剂检测和抑制鳞状细胞癌的组合物和方法。

5. 发明授权

US09040484B2 Production and delivery of a stable collagen 有权
标题翻译：生产和交付稳定的胶原蛋白
公开(公告)号：US09040484B2
公开(公告)日：2015-05-26
申请号：US14112444
申请日：2012-04-26
申请人： M. Peter Marinkovich , Alfred T. Lane , Jayakumar Rajadas
发明人： M. Peter Marinkovich , Alfred T. Lane , Jayakumar Rajadas
IPC分类号： A61K38/39 , A61M5/00 , C07K14/78 , C12N9/02 , A61M37/00 , C12N15/67
CPC分类号： C07K14/78 , A61K38/39 , A61M37/0015 , A61M2037/0023 , C12N9/0071 , C12N15/67
摘要： Improved methods are provided for the recombinant synthesis of collagen, particularly collagen VII, in host cell, and for therapeutic delivery of the same. The recombinant collagen is produced in a host cell that has increased levels of prolyl-4-hydroxylase, relative to basal cell levels. The collagen produced by the methods of the invention has increased numbers of modified proline residues, relative to a recombinant collagen produced in a host cell having basal levels of prolyl-4-hydroxylase. The increased proline modification provides for a collagen having increased stability, including increased in vivo stability.
摘要翻译：提供改进的方法用于在宿主细胞中重组合成胶原蛋白，特别是胶原VII，并且用于其治疗性递送。相对于基础细胞水平，在具有增加的脯氨酰-4-羟化酶水平的宿主细胞中产生重组胶原。通过本发明方法产生的胶原相对于在具有基础水平的脯氨酰-4-羟化酶的宿主细胞中产生的重组胶原具有增加的修饰脯氨酸残基数目。增加的脯氨酸修饰提供了具有增加的稳定性的胶原蛋白，包括增加的体内稳定性。

6. 发明授权

US07875277B2 Compositions and methods for inhibiting squamous cell carcinoma 有权
标题翻译：抑制鳞状细胞癌的组合物和方法
公开(公告)号：US07875277B2
公开(公告)日：2011-01-25
申请号：US12082906
申请日：2008-04-14
申请人： M. Peter Marinkovich
发明人： M. Peter Marinkovich
IPC分类号： A61K39/395
CPC分类号： C07K16/30 , A61K9/0019 , A61K2039/505 , C07K2317/73 , G01N33/57484 , G01N2333/78
摘要： The present invention relates to compositions and methods for detecting and inhibiting squamous cell carcinoma using agents that target the laminin 5 alpha 3 G4-G5 domain.
摘要翻译：本发明涉及使用靶向层粘连蛋白5α3G4-G5结构域的试剂检测和抑制鳞状细胞癌的组合物和方法。

7. 发明授权

US07851169B2 Compositions and methods for inhibiting squamous cell carcinoma 有权
标题翻译：抑制鳞状细胞癌的组合物和方法
公开(公告)号：US07851169B2
公开(公告)日：2010-12-14
申请号：US11943486
申请日：2007-11-20
申请人： M. Peter Marinkovich
发明人： M. Peter Marinkovich
IPC分类号： G01N33/574
CPC分类号： G01N33/57484 , A61K2039/505 , C07K16/30 , G01N2333/78
摘要： The present invention relates to compositions and methods for detecting and inhibiting squamous cell carcinoma using agents that target the laminin 5 alpha 3 G4-G5 domain.
摘要翻译：本发明涉及使用靶向层粘连蛋白5α3G4-G5结构域的试剂检测和抑制鳞状细胞癌的组合物和方法。

8. 发明授权

US07323551B2 Compositions and methods for inhibiting squamous cell carcinoma 有权
公开(公告)号：US07323551B2
公开(公告)日：2008-01-29
申请号：US10766317
申请日：2004-01-27
申请人： M. Peter Marinkovich
发明人： M. Peter Marinkovich
IPC分类号： C07K16/00
CPC分类号： G01N33/57484 , A61K2039/505 , C07K16/30 , G01N2333/78
摘要： The present invention relates to compositions and methods for detecting and inhibiting squamous cell carcinoma using agents that target the laminin 5 alpha 3 G4-G5 domain.

9. 发明申请

US20140107036A1 Production and Delivery of a Stable Collagen 有权
标题翻译：生产和交付稳定的胶原蛋白
公开(公告)号：US20140107036A1
公开(公告)日：2014-04-17
申请号：US14112444
申请日：2012-04-26
申请人： M. Peter Marinkovich , Alfred T. Lane , Jayakumar Rajadas
发明人： M. Peter Marinkovich , Alfred T. Lane , Jayakumar Rajadas
IPC分类号： C07K14/78 , A61M37/00 , A61K38/39
CPC分类号： C07K14/78 , A61K38/39 , A61M37/0015 , A61M2037/0023 , C12N9/0071 , C12N15/67
摘要： Improved methods are provided for the recombinant synthesis of collagen, particularly collagen VII, in host cell, and for therapeutic delivery of the same. The recombinant collagen is produced in a host cell that has increased levels of prolyl-4-hydroxylase, relative to basal cell levels. The collagen produced by the methods of the invention has increased numbers of modified proline residues, relative to a recombinant collagen produced in a host cell having basal levels of prolyl-4-hydroxylase. The increased proline modification provides for a collagen having increased stability, including increased in vivo stability.
摘要翻译：提供改进的方法用于重组合成胶原，特别是胶原VII，在宿主细胞中，并用于治疗性递送。相对于基础细胞水平，在具有增加的脯氨酰-4-羟化酶水平的宿主细胞中产生重组胶原。通过本发明方法产生的胶原相对于在具有基础水平的脯氨酰-4-羟化酶的宿主细胞中产生的重组胶原具有增加的修饰脯氨酸残基数目。增加的脯氨酸修饰提供了具有增加的稳定性的胶原蛋白，包括增加的体内稳定性。

10. 发明申请

US20100260764A1 COMPOSITIONS AND METHODS FOR INHIBITING SQUAMOUS CELL CARCINOMA 审中-公开
标题翻译：用于抑制细胞癌的组合物和方法
公开(公告)号：US20100260764A1
公开(公告)日：2010-10-14
申请号：US12733418
申请日：2008-08-28
申请人： M. Peter Marinkovich
发明人： M. Peter Marinkovich
IPC分类号： A61K39/395 , G01N33/53
CPC分类号： A61K39/39558 , A61K2039/505 , C07K16/18
摘要： The present invention relates to compositions and methods for detecting and inhibiting squamous cell carcinoma using agents that target the laminin 332 γ2 processed region polypeptide.
摘要翻译：本发明涉及使用靶向层粘连蛋白332γ2处理区域多肽的试剂检测和抑制鳞状细胞癌的组合物和方法。

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