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    • 8. 发明授权
    • Universal probe assay methods
    • 通用探针测定方法
    • US09353406B2
    • 2016-05-31
    • US13280214
    • 2011-10-24
    • Kenneth J. LivakJason A. A. WestRobert C. Jones
    • Kenneth J. LivakJason A. A. WestRobert C. Jones
    • C12Q1/68
    • C12Q1/682C12Q1/6876C12Q2600/178C12Q2525/155C12Q2525/307C12Q2537/161
    • Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.
    • 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面,通过扩增靶核酸序列以产生包含靶序列的扩增产物,与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列,从而产生扩增产物; 并且将第一检测探针与扩增产物杂交,所述第一检测探针包含与第一探针结合序列杂交的第一区段和与第二探针结合序列杂交的第二区段,从而产生标记的扩增产物 。