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1. 发明授权

US4404366A Beta-galactosyl-umbelliferone-labeled hapten conjugates 失效
标题翻译： β-半乳糖基 - 伞形酮标记的半抗原共轭物
公开(公告)号：US4404366A
公开(公告)日：1983-09-13
申请号：US284137
申请日：1981-07-16
申请人： Robert C. Boguslaski , John F. Burd , Robert J. Carrico
发明人： Robert C. Boguslaski , John F. Burd , Robert J. Carrico
IPC分类号： C07H17/075 , G01N33/533 , G01N33/535 , C07H15/24
CPC分类号： C07H17/075 , G01N33/533 , G01N33/535
摘要： An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula:G--D--Rwherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glycosidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or heterogeneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue. The improved assay is particularly suited to the determination of haptens, such as drugs, and antigenic proteins and polypeptides, including antibodies, following a homogeneous competitive binding assay format.CROSS-REFERENCE TO RELATED APPLICATION
摘要翻译：用于测定液体培养基中配体的改进的特异性结合测定方法和试剂，其使用具有下式的GDR的残基作为可酶切的底物标记，其中G是糖苷，D是染料指示剂部分，R是连接基团，其中标记残基与常规结合测定系统（例如配体，其类似物或其特异性结合配偶体）的结合成分共价结合。标记物的监测特征是在酶切割糖基与染料指示剂部分之间的糖苷键时可释放的产物（通常为氟或色原体）的释放。测定方法可以遵循均匀或异质的方式。优选的糖酮是β-半乳糖基，优选的染料指示剂部分是伞形酮残基。改进的测定法特别适合于均相竞争性结合测定形式之后测定半抗原，例如药物和抗原蛋白质和多肽，包括抗体。

2. 发明授权

US4279992A Specific binding assay employing an enzyme-cleavable substrate as label 失效
标题翻译：使用酶切割底物作为标记的特异性结合测定
公开(公告)号：US4279992A
公开(公告)日：1981-07-21
申请号：US87819
申请日：1979-10-23
申请人： Robert C. Boguslaski , John F. Burd , Robert J. Carrico
发明人： Robert C. Boguslaski , John F. Burd , Robert J. Carrico
IPC分类号： C07D209/48 , C07D233/78 , C07H17/075 , G01N33/533 , G01N33/535 , G01N33/54 , C12N9/96 , C12Q1/34 , C12Q1/44
CPC分类号： C07D209/48 , C07D233/78 , C07H17/075 , G01N33/533 , G01N33/535 , Y10S435/81 , Y10S435/961 , Y10S436/80 , Y10S436/815 , Y10S436/816
摘要： An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula:G--D--Rwherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glycosidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or heterogeneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue. The improved assay is particularly suited to the determination of haptens, such as drugs, and antigenic proteins and polypeptides, including antibodies, following a homogeneous competitive binding assay format.
摘要翻译：用于测定液体培养基中配体的改进的特异性结合测定方法和试剂，其使用具有下式的GDR的残基作为可酶切的底物标记，其中G是糖苷，D是染料指示剂部分，R是连接基团，其中标记残基与常规结合测定系统（例如配体，其类似物或其特异性结合配偶体）的结合成分共价结合。标记物的监测特征是在酶切割糖基与染料指示剂部分之间的糖苷键时可释放的产物（通常为氟或色原体）的释放。测定方法可以遵循均匀或异质的方式。优选的糖酮是β-半乳糖基，优选的染料指示剂部分是伞形酮残基。改进的测定法特别适合于均相竞争性结合测定形式之后测定半抗原，例如药物和抗原蛋白质和多肽，包括抗体。

3. 发明授权

US4226978A .beta.-Galactosyl-umbelliferone-labeled aminoglycoside antibiotics and intermediates in their preparation 失效
标题翻译： β-半乳糖苷 - 伞形酮 - 标记的氨基糖苷类抗生素及其制备中间体
公开(公告)号：US4226978A
公开(公告)日：1980-10-07
申请号：US886094
申请日：1978-03-13
申请人： Robert C. Boguslaski , Robert J. Carrico , John F. Burd
发明人： Robert C. Boguslaski , Robert J. Carrico , John F. Burd
IPC分类号： C07D209/48 , C07D233/78 , C07H17/075 , C07H21/00 , G01N33/533 , G01N33/535 , C07H15/22 , C07H17/04
CPC分类号： C07D209/48 , C07D233/78 , C07H17/075 , C07H21/00 , G01N33/533 , G01N33/535
摘要： .beta.-Galactosyl-umbelliferone labeled aminoglycoside antibiotics, e.g., gentamicin, useful in homogeneous and heterogeneous specific binding assays for such antibiotics in liquid media such as serum. Also provided are a .beta.-galactosyl-umbelliferone-carboxylic acid and salts thereof which are intermediates in a method of preparing the labeled antibiotics.
摘要翻译： β-半乳糖基 - 伞形酮酮标记的氨基糖苷类抗生素，例如庆大霉素，可用于在诸如血清的液体培养基中的这种抗生素的均相和异质特异性结合测定。还提供了β-半乳糖基 - 伞形酮 - 羧酸及其盐，其是制备标记抗生素的方法中的中间体。

4. 发明授权

US4331590A .beta.-Galactosyl-umbelliferone-labeled protein and polypeptide conjugates 失效
标题翻译： β-半乳糖基 - 伞形酮酮标记的蛋白质和多肽缀合物
公开(公告)号：US4331590A
公开(公告)日：1982-05-25
申请号：US147339
申请日：1980-05-06
申请人： Robert C. Bocuslaski , John F. Burd , Robert J. Carrico
发明人： Robert C. Bocuslaski , John F. Burd , Robert J. Carrico
IPC分类号： C07H17/075 , G01N33/533 , G01N33/535 , G01N33/58 , A61K39/385 , A61K39/44 , C07G7/00
CPC分类号： C07H17/075 , G01N33/533 , G01N33/535 , G01N33/582 , Y10S436/80 , Y10S436/805 , Y10S436/823 , Y10S530/806
摘要： An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula:G-D-Rwherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glycosidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or heterogeneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue. The improved assay is particularly suited to the determination of haptens, such as drugs, and antigenic proteins and polypeptides, including antibodies, following a homogeneous competitive binding assay format.
摘要翻译：用于测定液体培养基中配体的改进的特异性结合测定方法和试剂，其使用具有下式的GDR的残基作为可酶切的底物标记，其中G是糖苷，D是染料指示剂部分，R是连接基团，其中标记残基与常规结合测定系统（例如配体，其类似物或其特异性结合配偶体）的结合成分共价结合。标记物的监测特征是在酶切割糖基与染料指示剂部分之间的糖苷键时可释放的产物（通常为氟或色原体）的释放。测定方法可以遵循均匀或异质的方式。优选的糖酮是β-半乳糖基，优选的染料指示剂部分是伞形酮残基。改进的测定法特别适合于均相竞争性结合测定形式之后测定半抗原，例如药物和抗原蛋白质和多肽，包括抗体。

5. 发明授权

US5885839A Methods of determining initiation and variable end points for measuring a chemical reaction 失效
标题翻译：确定用于测量化学反应的起始点和可变终点的方法
公开(公告)号：US5885839A
公开(公告)日：1999-03-23
申请号：US842616
申请日：1997-04-15
申请人： Paul J. Lingane , John F. Burd , Karen A. Goins , Michael D. Goins
发明人： Paul J. Lingane , John F. Burd , Karen A. Goins , Michael D. Goins
IPC分类号： G01N21/86 , G01N33/00
CPC分类号： G01N21/8483 , Y10T436/112499 , Y10T436/115831 , Y10T436/142222 , Y10T436/143333 , Y10T436/144444
摘要： The present invention provides a method of using a reflectance-reading device to determine an initiation time point for measuring the chemical reaction of an analyte from a biological liquid sample on a test surface. The initiation time point is determined by using a device to read a reflectance of a test surface at a plurality of time points, and calculating the K/S ratio of the test surface at each time point according to the Kubelka-Munk equation. As the device continues to calculate a K/S ratio for each time point, the device monitors the rate of change of the K/S ratio with respect to time. The device then determines the initiation time point to be when the rate of change of the K/S ratio is maximal. The present invention also provides a method of measuring the concentration of an analyte on a test surface. The concentration is measured by determining an initiation time point according to the method described above and then measuring the concentration of the analyte at a variable end point. The variable end point is essentially determined to be the time at which the rate of change of a concentration parameter is less than a threshold value.
摘要翻译：本发明提供一种使用反射率读取装置来确定用于测量来自测试表面上的生物液体样品的分析物的化学反应的起始时间点的方法。通过使用设备读取多个时间点的测试表面的反射率并根据Kubelka-Munk方程计算每个时间点的测试表面的K / S比来确定起始时间点。当设备继续计算每个时间点的K / S比时，设备将监视K / S比率相对于时间的变化率。然后，当K / S比的变化率最大时，该装置确定起始时间点。本发明还提供了一种测量测试表面上分析物浓度的方法。通过根据上述方法确定起始时间点，然后测量分析物在可变终点的浓度来测量浓度。可变终点基本上被确定为浓度参数的变化率小于阈值的时间。

6. 发明授权

US4608336A #3B theophylline immunoassay employing 9-theophylline reagents 失效
标题翻译：＃3B茶碱免疫测定采用9-茶碱试剂
公开(公告)号：US4608336A
公开(公告)日：1986-08-26
申请号：US493609
申请日：1983-05-11
申请人： Jeffrey L. Benovic , Robert T. Buckler , John F. Burd , Thomas M. Li
发明人： Jeffrey L. Benovic , Robert T. Buckler , John F. Burd , Thomas M. Li
IPC分类号： C07D473/08 , C07K16/00 , C07K16/18 , G01N33/94 , G01N33/53
CPC分类号： C07K16/18 , C07D473/08 , C07K16/00 , G01N33/94 , A61K2039/6031 , A61K2039/6087 , Y10S435/805 , Y10S435/81 , Y10S435/961 , Y10S435/975 , Y10S436/808 , Y10S436/81 , Y10S436/815 , Y10S436/822 , Y10S436/823
摘要： Immunoassay method and reagent means for determining theophylline in biological fluids such as serum. The antibody employed in the method is prepared from an immunogen comprising theophylline coupled at its 9-position to an immunogenic carrier material.
摘要翻译：用于测定生物液体如血清中茶碱的免疫测定方法和试剂。在该方法中使用的抗体由包含茶碱在其9-位上与免疫原性载体材料偶联的免疫原制备。

7. 发明授权

US4273715A N-(hydroxyalkyl)-7-hydroxycoumarin-3-carboxamides 失效
标题翻译： N-（羟基烷基）-7-羟基香豆素-3-甲酰胺
公开(公告)号：US4273715A
公开(公告)日：1981-06-16
申请号：US140019
申请日：1980-04-14
申请人： Robert T. Buckler , John F. Burd
发明人： Robert T. Buckler , John F. Burd
IPC分类号： C07D311/16 , G01N33/542 , C07D
CPC分类号： C07D311/16 , Y10S435/968
摘要： Title compounds of the formula: ##STR1## wherein R is hydroxy substituted lower alkyl. The compounds have advantageous water solubility and spectral characteristics for use as instrument calibrators for certain homogeneous fluorescent immunoassays.
摘要翻译：下式的标题化合物：其中R是羟基取代的低级烷基。该化合物具有有利的水溶性和光谱特性，用作某些均匀荧光免疫测定的仪器校准物。

8. 发明授权

US4650771A Immunogens, antibodies, labeled conjugates, and related derivatives for lidocaine and analogs thereof 失效
标题翻译：免疫原，抗体，标记的缀合物和利多卡因及其类似物的相关衍生物
公开(公告)号：US4650771A
公开(公告)日：1987-03-17
申请号：US548647
申请日：1983-11-04
申请人： Robert T. Buckler , John F. Burd , Stephan G. Thompson
发明人： Robert T. Buckler , John F. Burd , Stephan G. Thompson
IPC分类号： G01N33/53 , A61K39/385 , C07H21/00 , C07K14/00 , C07K14/76 , C07K16/00 , G01N33/531 , G01N33/94 , G01N33/536 , A61K39/00
CPC分类号： C07H21/00 , G01N33/531 , Y10S435/961 , Y10S436/815 , Y10S436/822 , Y10S436/823
摘要： Immunogens for preparing antibodies against the drug lidocaine and related compounds, labeled conjugates, synthetic intermediates, and the use of such antibodies and labeled conjugates in immunoassays for determining lidocaine and such related compounds. The immunogens comprise lidocaine or an analog thereof coupled through one of the aromatic methyl groups to a conventional immunogenic carrier. The antibodies and labeled conjugates are particularly useful in homogeneous nonradioisotopic immunoassays for measuring lidocaine or its analogs in biological fluids such as serum.
摘要翻译：用于制备针对药物利多卡因及相关化合物，标记的缀合物，合成中间体的抗体的免疫原，以及用于测定利多卡因和这种相关化合物的免疫测定中使用此类抗体和标记的缀合物。免疫原包括通过芳族甲基之一与常规免疫原性载体偶联的利多卡因或其类似物。抗体和标记的缀合物在用于测量生物液体如血清中的利多卡因或其类似物的均相非放射性同位素免疫测定中特别有用。

9. 发明授权

US4460772A 6-[N-(.omega.-Carboxyalkyl)amino]-1,3-dimethyl-5-formamidouracils 失效
标题翻译： 6- [N-（ω-羧基烷基）氨基] -1,3-二甲基-5-甲酰尿嘧啶
公开(公告)号：US4460772A
公开(公告)日：1984-07-17
申请号：US493632
申请日：1983-05-11
申请人： Jeffrey L. Benovic , Robert T. Buckler , John F. Burd , Thomas M. Li
发明人： Jeffrey L. Benovic , Robert T. Buckler , John F. Burd , Thomas M. Li
IPC分类号： C07D473/08 , C07K16/00 , C07K16/18 , G01N33/94 , C07D239/54
CPC分类号： C07D473/08 , C07K16/00 , C07K16/18 , G01N33/94 , A61K2039/6031 , A61K2039/6087 , Y10S435/961 , Y10S530/807
摘要： Intermediates in the preparation of 9-position derivatives of theophylline useful in synthesizing theophylline immunogens and labeled conjugates. The labeled conjugates and the antibodies produced against the immunogens are particularly useful in homogeneous nonradioisotopic immunoassay for measuring theophylline in biological fluids such as serum.
摘要翻译：制备茶碱9位衍生物的中间体，可用于合成茶碱免疫原和标记的缀合物。标记的缀合物和针对免疫原产生的抗体在用于测量生物液体如血清中茶碱的均相非放射免疫测定中特别有用。

10. 发明授权

US4318981A Homogeneous specific binding assay employing an intramolecularly modulated photogenic enzyme substrate label 失效
标题翻译：使用分子内调节的光致酶底物标签的均相特异性结合测定
公开(公告)号：US4318981A
公开(公告)日：1982-03-09
申请号：US143497
申请日：1980-04-24
申请人： John F. Burd , Thomas M. Li
发明人： John F. Burd , Thomas M. Li
IPC分类号： G01N33/58 , C12Q1/66
CPC分类号： G01N33/581 , Y10S435/971
摘要： A homogeneous specific binding assay method and reagent means for determining a ligand, such as a hapten, antigen or antibody, in, or the ligand binding capacity of, a liquid medium employing a labeled conjugate which upon enzymatic cleavage produces a detectable indicator product. The improvement comprises employing as the label component in the labeled conjugate, a residue having the formula:P--X--M--Rwherein P is a photophore, e.g., a fluorescer, which emits light upon exposure to excitation means, X is an enzymatically cleavable linkage, M is a modulator, e.g., a quencher, for said light emission of the photophore, and R is a linking group to the binding component, e.g., the ligand or analog thereof, in the labeled conjugate. The labeled conjugate and the cleaved indicator product emit substantially different amounts of light upon excitation due to modulation of the emission of photophore P by the proximity of modulator M in the labeled conjugate.
摘要翻译：使用标记的缀合物的液体培养基中的配体，例如半抗原，抗原或抗体或其配体结合能力的均相特异性结合测定方法和试剂方法，其在酶裂解时产生可检测的指示剂产物。改进包括在标记的缀合物中使用标记成分，具有下式的残基：PXMR，其中P是光致抗原，例如荧光素，其在暴露于激发装置时发光，X是可酶切割的键，M是调节剂，例如猝灭剂，用于所述光致发光的发光，R是与标记的缀合物中的结合组分（例如其配体或类似物）的连接基团。标记的缀合物和切割的指示剂产物由于通过调节剂M在标记的缀合物中的接近而调制光致磷P的发射而在激发时发射基本上不同的光量。

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