会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 3. 发明授权
    • Methods for increasing the efficiency of recombinant AAV product
    • 提高重组AAV产品效率的方法
    • US06548286B1
    • 2003-04-15
    • US09060102
    • 1998-04-14
    • Richard Jude SamulskiXiao XiaoRichard Snyder
    • Richard Jude SamulskiXiao XiaoRichard Snyder
    • C12N701
    • C12N15/86A61K48/00C07K14/005C12N7/00C12N2710/10322C12N2710/10343C12N2750/14121C12N2750/14122C12N2750/14143C12N2750/14152
    • The present invention relates to methods and compositions for increasing the production of high titre stocks of recombinant AAV (rAAV) through regulation of expression of the AAV REP and CAP proteins. The methods and compositions of the invention are based on the observation that the low level expression of the AAV REP protein increases the production of AAV viral capsid protein and efficiency of packaging resulting in production of higher titre recombinant viral stocks. The invention encompasses recombinant AAV vectors that direct the expression of AAV REP and CAP proteins and the use of such vectors for the production of novel stable cell lines capable of generating high titre rAAV vectors. The invention provides methods for regulating the expression of the AAV REP gene at the transcriptional and post-translational level. The methods and compositions of the invention can be used to produce high titre stocks of rAAV which can be used in gene therapy for the purpose of transferring genetic information into appropriate host cells for the management and correction of human diseases including inherited and acquired disorders.
    • 本发明涉及通过调节AAV REP和CAP蛋白的表达来增加重组AAV(rAAV)的高滴度储备的生产的方法和组合物。 本发明的方法和组合基于AAV REP蛋白的低水平表达增加AAV病毒衣壳蛋白的产生和包装效率的观察,导致产生较高滴度的重组病毒种群。 本发明包括引导AAV REP和CAP蛋白的表达的重组AAV载体,以及这些载体用于产生能产生高滴度rAAV载体的新型稳定细胞系的用途。 本发明提供了在转录和翻译后水平调节AAV REP基因表达的方法。 本发明的方法和组合物可用于生产高滴度的rAAV,其可用于基因治疗,以将遗传信息转移到合适的宿主细胞中,用于管理和矫正包括遗传和获得性疾病在内的人类疾病。
    • 5. 发明授权
    • Filter assembly for cleaning cooling air for engines
    • 用于清洁发动机冷却空气的过滤器组件
    • US6162269A
    • 2000-12-19
    • US299320
    • 1999-04-26
    • Roy E. GreenleesRichard SnyderKenneth S. SchultzR. Ryan Greenlees
    • Roy E. GreenleesRichard SnyderKenneth S. SchultzR. Ryan Greenlees
    • F01P11/12F02B77/00
    • F01P11/12Y10S55/28
    • An engine filter assembly for cleaning cooling air for an internal-combustion engine for use in high-debris environment such as in roofing-removing machines includes a filter housing attached to the machine at a remote position. The filter housing houses a two-stage air filter that consists of a cylindrical-shaped filter element with an outer pre-filter covering. Both the carburetor air inlet and a fan housing leading to the cooling fan on the engine are connected to the filter housing by tubing. A shield protects the engine from direct contact with loose or air-borne materials during the operation of the machine. A thermal sensing device reads the temperature of the engine and automatically shuts down the engine if the temperature rises beyond specification, reducing the chance of engine failure.
    • 一种用于清洁用于高碎片环境的内燃机的冷却空气的发动机过滤器组件,例如在屋顶除尘机中包括在远程位置附接到机器的过滤器壳体。 过滤器壳体容纳两级空气过滤器,其由具有外部预过滤器覆盖物的圆柱形过滤元件组成。 化油器空气入口和通向发动机上的冷却风扇的风扇壳都通过管道连接到过滤器壳体。 屏蔽罩可保护发动机在机器运行过程中与松散或空气传播的材料直接接触。 如果温度升高超过规格,热感测装置读取发动机的温度并自动关闭发动机,减少发动机故障的机会。