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1. 发明授权

US06277617B1 Chemically modified enzymes 失效
标题翻译：化学修饰的酶
公开(公告)号：US06277617B1
公开(公告)日：2001-08-21
申请号：US08978509
申请日：1997-11-25
申请人： Richard R. Bott , Thomas P. Graycar , J. Bryan Jones , Colin Mitchinson
发明人： Richard R. Bott , Thomas P. Graycar , J. Bryan Jones , Colin Mitchinson
IPC分类号： A61K3848
CPC分类号： C11D3/38663 , C12N9/00 , C12N9/54
摘要： Modified enzymes are provided in which at least one amino acid, such as asparagine, leucine, methionine or serine, of an enzyme is replaced with a cysteine and the thiol hydrogen is replaced with a substituent group providing a thiol side chain selected from the group consisting of: a) —SR1R2, wherein R1 is an alkyl and R2 is a charged or polar moiety; b) —SR3, wherein R3 is a substituted or unsubstituted phenyl; c) —SR4, wherein R4 is substituted or unsubstituted cyclohexyl; d) —SR5, wherein R5 is C10-C15 alkyl; and e) —SR6 wherein R6 is a C1-6 alkyl. Also, methods of producing the modified enzymes are provided, as well as detergent and feed additives and a composition for the treatment of a textile. A method for using the modified enzymes in organic synthesis is additionally provided. Further, modified enzymes having improved activity, altered pH profile and/or wash performance are provided.
摘要翻译：提供了修饰的酶，其中酶的至少一个氨基酸，例如天冬酰胺，亮氨酸，甲硫氨酸或丝氨酸被半胱氨酸替代，硫醇氢被提供选自以下的硫醇侧链的取代基取代： a）-SR1R2，其中R1是烷基，R2是带电荷或极性部分; b）-SR3，其中R3是取代或未取代的苯基; c）-SR4，其中R4是取代或未取代的环己基; d） -SR 5，其中R 5为C 10 -C 15烷基; 安德）-SR6，其中R6是C1-6烷基。此外，提供了制备修饰酶的方法，以及洗涤剂和饲料添加剂以及用于处理织物的组合物。另外提供了在有机合成中使用修饰的酶的方法。此外，提供了具有改善的活性，改变的pH曲线和/或洗涤性能的修饰的酶。

2. 发明授权

US07250281B2 Subtilisin variants 失效
标题翻译：枯草杆菌蛋白酶变体
公开(公告)号：US07250281B2
公开(公告)日：2007-07-31
申请号：US10323324
申请日：2002-12-18
申请人： Thomas P. Graycar , Richard R. Bott , Lori J. Wilson
发明人： Thomas P. Graycar , Richard R. Bott , Lori J. Wilson
IPC分类号： C12N9/56 , C12N15/57 , C12D15/74 , C11D3/386
CPC分类号： C11D3/38609 , C12N9/54
摘要： Novel carbonyl hydrolase variants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The variant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such variant carbonyl hydrolases have properties which are different from those of the precursor hydrolase, such as altered proteolytic activity, altered stability, etc. The substituted amino acid residues correspond to positions +76 in combination with one or more of the following residues +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265 and/or +274 in Bacillus amyloliquefaciens subtilisin.
摘要翻译：公开了衍生自天然存在或重组非人羰基水解酶的DNA序列的新型羰基水解酶变体。变体羰基水解酶通常通过体外修饰编码天然存在或重组羰基水解酶的前体DNA序列获得，以产生前体羰基水解酶的氨基酸序列中的多个氨基酸残基的取代。这种变体羰基水解酶具有与前体水解酶不同的性质，例如改变的蛋白水解活性，改变的稳定性等。取代的氨基酸残基对应于与一个或多个以下残基组合的位置+76， +101，+103，+104，+107，+123，+27，+105，+ 109，+126，+128，+135，+ 156，+166，+ 195，+ 197，+ 204，+ 206 ，+210，+ 216，+ 217，+ 218，+ 222，+ 260，+ 265和/或+ 274的解淀粉芽孢杆菌枯草杆菌蛋白酶。

3. 发明授权

US5316941A Non-human carbonyl hydrolase mutants, DNA sequences and vectors encoding same and hosts transformed with said vectors 失效
标题翻译：非人羰基水解酶突变体，DNA序列和编码相同的载体以及用所述载体转化的宿主
公开(公告)号：US5316941A
公开(公告)日：1994-05-31
申请号：US896818
申请日：1992-04-29
申请人： David A. Estell , Robert M. Caldwell , Richard R. Bott , Thomas P. Graycar
发明人： David A. Estell , Robert M. Caldwell , Richard R. Bott , Thomas P. Graycar
IPC分类号： C12N9/56 , C12N15/00 , C12N9/54
CPC分类号： C12N9/54
摘要： There are described certain DNA sequences which encode subtilisins wherein the amino acid sequence of such substilisins has been modified at a position equivalent to +225 in Bacillus amyloliquefaciens, such that an amino acid selected from the group consisting of alanine, leucine, methionine, glutamine, valine and serine, has been substituted for the amino acid residues naturally occuring at such position.
摘要翻译：描述了编码枯草杆菌蛋白酶的某些DNA序列，其中这种变形蛋白酶的氨基酸序列已在解淀粉芽孢杆菌中相当于+225的位置被修饰，使得选自丙氨酸，亮氨酸，甲硫氨酸，谷氨酰胺，缬氨酸和丝氨酸已被替代在此位置天然存在的氨基酸残基。

4. 发明授权

US06586221B2 Subtilisin variants multiply-substituted subtilising variants having a substitution at position 76 失效
标题翻译：枯草杆菌蛋白酶变体在第76位具有取代的多取代的枯草芽孢杆菌变体
公开(公告)号：US06586221B2
公开(公告)日：2003-07-01
申请号：US08322678
申请日：1994-10-13
申请人： Thomas P. Graycar , Richard R. Bott , Lori J. Wilson
发明人： Thomas P. Graycar , Richard R. Bott , Lori J. Wilson
IPC分类号： C12N950
CPC分类号： C11D3/38609 , C12N9/54
摘要： Novel carbonyl hydrolase variants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The variant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such variant carbonyl hydrolases have properties which are different from those of the precursor hydrolase, such as altered proteolytic activity, altered stability, etc. The substituted amino acid residues correspond to positions +76 in combination with one or more of the following residues +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265 and/or +274 in Bacillus amyloliquefaciens subtilisin.
摘要翻译：公开了衍生自天然存在或重组非人羰基水解酶的DNA序列的新型羰基水解酶变体。变体羰基水解酶通常通过体外修饰编码天然存在或重组羰基水解酶的前体DNA序列获得，以产生前体羰基水解酶的氨基酸序列中的多个氨基酸残基的取代。这种变体羰基水解酶具有与前体水解酶不同的性质，例如改变的蛋白水解活性，改变的稳定性等。取代的氨基酸残基对应于与一个或多个以下残基组合的位置+76， +101，+103，+104，+107，+123，+27，+105，+ 109，+126，+128，+135，+ 156，+166，+ 195，+ 197，+ 204，+ 206 ，+210，+ 216，+ 217，+ 218，+ 222，+ 260，+ 265和/或+ 274的解淀粉芽孢杆菌枯草杆菌蛋白酶。

5. 发明授权

US5155033A Subtilisins modified at position 225 resulting in a shift in catalytic activity 失效
标题翻译：在225位修饰的枯草杆菌蛋白酶导致催化活性的变化
公开(公告)号：US5155033A
公开(公告)日：1992-10-13
申请号：US294340
申请日：1989-01-06
申请人： David A. Estell , Robert M. Caldwell , Richard R. Bott , Thomas P. Graycar
发明人： David A. Estell , Robert M. Caldwell , Richard R. Bott , Thomas P. Graycar
IPC分类号： C12N9/56
CPC分类号： C12N9/54
摘要： There are described certain subtilisins wherein the amino acid sequence of such subtilisins has been modified at a position equivalent to +225 in Bacillus amyloliquefaciens, such that an amino acid selected from the group consisting of alanine, leucine, methionine, glutamine, valine, and serine, has been substituted for the amino acid residue naturally occurring at such position.
摘要翻译：描述了某些枯草杆菌蛋白酶，其中这种枯草杆菌蛋白酶的氨基酸序列已经在解淀粉芽孢杆菌中相当于+225的位置被修饰，使得选自丙氨酸，亮氨酸，甲硫氨酸，谷氨酰胺，缬氨酸和丝氨酸的氨基酸已取代天然存在于该位置的氨基酸残基。

6. 发明授权

US08592194B2 Glucoamylase variants with altered properties 有权
标题翻译：具有改变性质的葡糖淀粉酶变体
公开(公告)号：US08592194B2
公开(公告)日：2013-11-26
申请号：US12681612
申请日：2008-04-08
申请人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
发明人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
IPC分类号： C12N9/00 , C12N9/34 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N5/10
摘要： The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolyzing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells.
摘要翻译：本发明涉及变体葡糖淀粉酶，其中与相应的母体葡糖淀粉酶相比，变体具有改变的性质（例如改善的热稳定性和/或比活性）。本发明还涉及包含变体葡糖淀粉酶（例如淀粉水解组合物）的酶组合物; 包含编码变体的多核苷酸的DNA构建体和在宿主细胞中产生变体葡糖淀粉酶的方法。

7. 发明申请

US20130102035A1 VARIANTS OF GLUCOAMYLASE 有权
公开(公告)号：US20130102035A1
公开(公告)日：2013-04-25
申请号：US13806398
申请日：2011-06-30
申请人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
发明人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
IPC分类号： C12N9/26 , C12P19/20
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.

8. 发明授权

US07982000B2 Composition comprising various proteorhodopsins and/or bacteriorhodopsins and use thereof 有权
标题翻译：包含各种蛋白质视紫红质和/或细菌视紫红质的组合物及其用途
公开(公告)号：US07982000B2
公开(公告)日：2011-07-19
申请号：US11569862
申请日：2005-06-09
申请人： Richard R. Bott , Rasmus B. Jensen , Bradley R. Kelemen , Donald E. Ward, II , Gregory M. Whited
发明人： Richard R. Bott , Rasmus B. Jensen , Bradley R. Kelemen , Donald E. Ward, II , Gregory M. Whited
IPC分类号： C07K17/00 , G11B11/00 , G11B7/085 , G11B7/00 , G11B5/84 , G11B7/24 , B32B3/02 , G03F7/00 , G02F1/09
CPC分类号： G11C7/00 , C07K14/215 , C09D11/037 , C09D11/50 , G11B7/242 , Y10S430/146 , Y10T428/249921 , Y10T428/31623 , Y10T428/31685 , Y10T428/31725 , Y10T428/31783
摘要： The present invention provides a solid material comprising an immobilized mixture of two or more proteorhodopsins, two or more bacteriorhodopsins, or one or more bacteriorhodopsin and one or more proteorhodopsins. The proteorhodopsins are selected from the group consisting of all-trans-retinal-containing proteorhodopsins and retinal analog-containing proteorhodopsins; all of which have absorption spectra that do not overlap. The bacteriorhodopsins are selected from the group consisting of all-trans-retinal-containing bacteriorhodopsins and retinal analog-containing bacteriorhodopsins; all of which have absorption spectra that do not overlap. The present invention also provides an optical information carrier, such as an optical data storage material and a fraud-proof optical data carrier, comprising the above-described solid material and a substrate selected from the group consisting of glass, paper, metal, fabric material, and plastic material, wherein said solid material is deposited on said substrate. The present invention further provides security ink comprising one or more hydrophilic polymers and a mixture of various photochromic materials.
摘要翻译：本发明提供了一种固体材料，其包含两种或多种蛋白质视紫红质，两种或更多种细菌视紫红质，或一种或多种细菌视紫红质和一种或多种蛋白质视紫质的固定化混合物。蛋白质视紫红质选自全反式视黄醛的蛋白质视黄素和含视网膜类似物的蛋白质视紫红质; 所有这些都具有不重叠的吸收光谱。细菌视紫红质选自全反式视网膜含细菌视紫红质和视网膜类似物的细菌视紫红质; 所有这些都具有不重叠的吸收光谱。本发明还提供一种光学信息载体，例如光学数据存储材料和防欺诈光学数据载体，其包括上述固体材料和选自玻璃，纸，金属，织物材料的基材和塑料材料，其中所述固体材料沉积在所述基底上。本发明还提供了包含一种或多种亲水聚合物和各种光致变色材料的混合物的安全油墨。

9. 发明申请

US20110020899A1 GLUCOAMYLASE VARIANTS WITH ALTERED PROPERTIES 有权
标题翻译：具有更改属性的葡萄糖酶变体
公开(公告)号：US20110020899A1
公开(公告)日：2011-01-27
申请号：US12681612
申请日：2008-04-08
申请人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
发明人： Wolfgang Aehle , Richard R. Bott , Igor Nikolaev , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
IPC分类号： C12N9/34 , C07H21/04 , C12N5/10
CPC分类号： C12N5/10
摘要： The present invention relates to variant glucoamylases wherein the variant has altered properties (e.g., improved thermostability and/or specific activity) compared to a corresponding parent glucoamylase. The present invention also relates to enzyme compositions comprising a variant glucoamylase (e.g., starch hydrolyzing compositions); DNA constructs comprising polynucleotides encoding the variants and methods of producing the variant glucoamylases in host cells
摘要翻译：本发明涉及变体葡糖淀粉酶，其中与相应的母体葡糖淀粉酶相比，变体具有改变的性质（例如改善的热稳定性和/或比活性）。本发明还涉及包含变体葡糖淀粉酶（例如淀粉水解组合物）的酶组合物; 包含编码变体的多核苷酸的DNA构建体和在宿主细胞中产生变体葡糖淀粉酶的方法

10. 发明申请

US20090275080A1 Glucoamylase variants with altered properties 有权
标题翻译：具有改变性质的葡糖淀粉酶变体
公开(公告)号：US20090275080A1
公开(公告)日：2009-11-05
申请号：US12292563
申请日：2008-11-20
申请人： Wolfgang Aehle , Richard R. Bott , Martijn Scheffers , Piet Van Solingen
发明人： Wolfgang Aehle , Richard R. Bott , Martijn Scheffers , Piet Van Solingen
IPC分类号： C12P21/06 , C12N9/34 , C12N5/00 , C12N15/63 , C07H21/04
CPC分类号： C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译：本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。

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