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1. 发明授权

US5478745A Recombinant viral vector system 失效
标题翻译：重组病毒载体系统
公开(公告)号：US5478745A
公开(公告)日：1995-12-26
申请号：US989841
申请日：1992-12-04
申请人： Richard J. Samulski , Xiao Xiao
发明人： Richard J. Samulski , Xiao Xiao
IPC分类号： C12N15/09 , A61K48/00 , C12N7/00 , C12N7/01 , C12N15/864 , C12P21/02 , C12R1/91 , C12R1/92 , C12N15/86 , C12N15/11
CPC分类号： C12N15/86 , A61K48/00 , C12N2750/14143
摘要： The present invention relates to a system for replication and encapsidation of recombinant DNA fragments into virus particles comprised of adenovirus associated viral (AAV) capsid proteins. The invention provides a means of obtaining recombinant viral stocks that may be used to treat patients suffering from genetic diseases.
摘要翻译：本发明涉及用于将重组DNA片段复制和包封成由腺病毒相关病毒（AAV）衣壳蛋白组成的病毒颗粒的系统。本发明提供获得可用于治疗患有遗传疾病的患者的重组病毒种群的方法。

2. 发明申请

US20090298922A1 AAV TRANSDUCTION OF MUSCLE TISSUE 审中-公开
标题翻译：肌肉组织的AAV转导
公开(公告)号：US20090298922A1
公开(公告)日：2009-12-03
申请号：US12537806
申请日：2009-08-07
申请人： Xiao Xiao , Richard J. Samulski , Juan Li
发明人： Xiao Xiao , Richard J. Samulski , Juan Li
IPC分类号： A61K48/00
CPC分类号： C12N15/86 , A61K48/00 , C12N2750/14143
摘要： A method of expressing a gene product in the muscle tissue of an animal, which comprises administering a recombinant AAV vector to the muscle tissue of the animal, wherein the vector comprises a non-AAV gene of interest ligated into an AAV vector genome.
摘要翻译：一种在动物的肌肉组织中表达基因产物的方法，其包括向所述动物的肌肉组织施用重组AAV载体，其中所述载体包含连接到AAV载体基因组中的目的非AAV基因。

3. 发明申请

US20080240612A1 NON-OVERLAP REGION BASED AUTOMATIC GLOBAL ALIGNMENT FOR RING CAMERA IMAGE MOSAIC 有权
标题翻译：基于非重叠区域的自动全球对准摄像机图像MOSAIC
公开(公告)号：US20080240612A1
公开(公告)日：2008-10-02
申请号：US11694778
申请日：2007-03-30
申请人： Luhong Liang , Xiao Xiao , Ying Jia , Steve W. Deutsch
发明人： Luhong Liang , Xiao Xiao , Ying Jia , Steve W. Deutsch
IPC分类号： G06K9/36
CPC分类号： G06T7/85 , G06T2207/10021 , G06T2207/30208
摘要： Various embodiments are directed to non-overlap region based automatic global alignment for ring camera image mosaic. The non-overlap region based homography calculation may be based on feature points of a planar target appearing in a non-overlap region among images captured by a multi-camera based video capture device. Other embodiments are described and claimed.
摘要翻译：各种实施例涉及用于环形摄像机图像拼接的基于非重叠区域的自动全局对准。基于非重叠区域的单应性计算可以基于出现在由基于多摄像机的视频捕获设备捕获的图像之中的非重叠区域中的平面目标的特征点。描述和要求保护其他实施例。

4. 发明授权

US11854156B2 Method and system of multi-pass iterative closest point (ICP) registration in automated facial reconstruction 有权
公开(公告)号：US11854156B2
公开(公告)日：2023-12-26
申请号：US17557446
申请日：2021-12-21
申请人： Mathew Powers , Xiao Xiao , William A. Kennedy
发明人： Mathew Powers , Xiao Xiao , William A. Kennedy
IPC分类号： G06T19/20 , G06T5/20 , G06V40/16 , G06T11/60
CPC分类号： G06T19/20 , G06T5/20 , G06T11/60 , G06V40/165 , G06V40/171
摘要： In one aspect, a computerized method for implementing a multi-pass iterative closest point (ICP) registration in an automated facial reconstruction process includes the step of providing an automated facial reconstruction process. The method includes the step of detecting that a face tracking application programming interface (API) misalignment has occurred during the automated facial reconstruction process. The method includes the step of implementing a multi-pass ICP process on the face tracking API misalignment by the following steps. One step includes obtaining a first point cloud (PC1) of the automated facial reconstruction process. The method includes the step of obtaining a second point cloud (PC2) of the automated facial reconstruction process. The method includes the step of obtaining a first face mask (mask1) of the automated facial reconstruction process. The method includes the step of obtaining a second face mask (mask2) of the automated facial reconstruction process. The method includes the step of performing a first point cloud (PC1)-to-mask ICP operation by inputting and utilizing PC1 and mask1. A transformation output of the first PC-to-mask ICP operation is provided to a third PC-to-PC ICP and a fourth PC-to-PC ICP. The method includes the step of performing a second PC-to-mask ICP on PC2 and mask2. A transformation output of the second PC-to-mask ICP step is provided to a second PC-to-PC ICP and to the third PC-to-PC ICP. The method includes the step of performing a first PC-to-PC ICP on the PC1 and the PC2. The first PC-to-PC ICP outputs a first transformation of the first PC-to-PC ICP and a first error value of the first PC-to-PC ICP. The method includes the step of performing a second PC-to-PC ICP on the transformation output by the second PC-to-mask ICP and the PC1. The second PC-to-PC ICP outputs a second transformation of the second PC-to-PC ICP and a second error value of the second PC-to-PC ICP. The method includes the step of performing a third PC-to-PC ICP on the transformation output of the first PC-to-mask ICP and the transformation output of the second PC-to-mask ICP. The third PC-to-PC ICP outputs a third transformation of the third PC-to-PC ICP and a third error value of the third PC-to-PC ICP. The method includes the step of performing a fourth PC-to-PC ICP on the transformation output of the first PC-to-mask ICP and the PC2. The fourth PC-to-PC ICP outputs a fourth transformation of the fourth PC-to-PC ICP and a fourth error value of the fourth PC-to-PC ICP.

5. 发明授权

US08809058B2 Helper virus-free AAV production 有权
标题翻译：帮助无病毒的AAV生产
公开(公告)号：US08809058B2
公开(公告)日：2014-08-19
申请号：US12140820
申请日：2008-06-17
申请人： Forrest K. Ferrari , Xiao Xiao , Richard Jude Samulski
发明人： Forrest K. Ferrari , Xiao Xiao , Richard Jude Samulski
IPC分类号： C12N15/63 , C12N15/64 , C12N15/861 , C12N15/86 , C12N15/864 , A61K48/00
CPC分类号： C12N7/00 , C07K14/005 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2750/14143 , C12N2750/14152
摘要： A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
摘要翻译：描述了生产基本上不含污染辅助病毒的腺相关病毒种群和重组腺相关病毒种群的方法。该方法利用辅助病毒载体的转染来代替常规方法中使用的辅助病毒感染。

6. 发明授权

US08273344B2 Recombinant adeno-associated virus expressing human antisense gene CyP2J2 and its preparation methods 有权
标题翻译：表达人反义基因CyP2J2的重组腺相关病毒及其制备方法
公开(公告)号：US08273344B2
公开(公告)日：2012-09-25
申请号：US11720035
申请日：2005-11-28
申请人： Daowen Wang , Jiangang Jiang , Xiao Xiao
发明人： Daowen Wang , Jiangang Jiang , Xiao Xiao
IPC分类号： C12N15/864 , C12N15/113 , C12N15/09 , C12N15/00
CPC分类号： C12N15/86 , A61K35/768 , C12N15/1137 , C12N2310/11 , C12N2310/111 , C12N2750/14132 , C12N2750/14143
摘要： A recombinant adeno-associated virus (rAAV) expressing antisense human CYP2J2 gene produced by a method of co-transfection into 293 cell by calcium phosphate precipitation, the rAAV having the following three plasmids: a) pXX2: a packing plasmid having a nucleotide sequence encoding AAV Rep and Cap protein, wherein the nucleotide sequence is operably linked upstream and downstream to a p5 promoter respectively to increase expression 15-fold; b) pXXUF1-anti2J2: an eukaryotic expression vector having a CMV promoter, a NotI site for insertion of target genes, the CYP2J2 gene oriented in the 3′ to 5′ direction, and inverted terminal repeats (ITRs); and c) pXX6: a helper plasmid deleted of the pathogenic gene sequences of adenovirus and having an E1A, an E2A, and a VA1 RNA gene of adenovirus. Also provided is a method of preparing the rAAV.
摘要翻译：通过磷酸钙沉淀共转染293细胞的方法产生的表达反义人CYP2J2基因的重组腺相关病毒（rAAV），具有以下三种质粒的rAAV：a）pXX2：具有编码 AAV Rep和Cap蛋白，其中所述核苷酸序列分别可操作地连接到p5启动子的上游和下游，以增加15倍的表达; b）pXXUF1-anti2J2：具有CMV启动子，用于插入靶基因的NotI位点，3'至5'方向取向的CYP2J2基因和反向末端重复（ITR）的真核表达载体; 和c）pXX6：缺失腺病毒的病原性基因序列的辅助质粒，并具有腺病毒的E1A，E2A和VA1 RNA基因。还提供了制备rAAV的方法。

7. 发明授权

US07961936B2 Non-overlap region based automatic global alignment for ring camera image mosaic 有权
标题翻译：基于非重叠区域的自动全局对准环形摄像机图像拼接
公开(公告)号：US07961936B2
公开(公告)日：2011-06-14
申请号：US11694778
申请日：2007-03-30
申请人： Luhong Liang , Xiao Xiao , Ying Jia , Steve W. Deutsch
发明人： Luhong Liang , Xiao Xiao , Ying Jia , Steve W. Deutsch
IPC分类号： G06K9/80 , G06T15/00
CPC分类号： G06T7/85 , G06T2207/10021 , G06T2207/30208
摘要： Various embodiments are directed to non-overlap region based automatic global alignment for ring camera image mosaic. The non-overlap region based homography calculation may be based on feature points of a planar target appearing in a non-overlap region among images captured by a multi-camera based video capture device. Other embodiments are described and claimed.
摘要翻译：各种实施例涉及用于环形摄像机图像拼接的基于非重叠区域的自动全局对准。基于非重叠区域的单应性计算可以基于出现在由基于多摄像机的视频捕获设备捕获的图像之中的非重叠区域中的平面目标的特征点。描述和要求保护其他实施例。

8. 发明申请

US20110104120A1 Directed Evolution and In Vivo Panning of Virus Vectors 有权
标题翻译：导向进化和病毒载体的体内淘选
公开(公告)号：US20110104120A1
公开(公告)日：2011-05-05
申请号：US12990460
申请日：2009-04-29
申请人： Xiao Xiao , Lin Yang
发明人： Xiao Xiao , Lin Yang
IPC分类号： A61K35/76 , C07H21/00 , C12N15/63 , C12N7/00 , C07K14/025 , C12N7/01 , A61K31/7088 , C12N15/86 , C12Q1/70 , C12N5/10 , A61P9/04 , A61P21/00
CPC分类号： C12N15/86 , A61K35/76 , A61K38/00 , A61K38/1719 , A61K48/0058 , C07K14/005 , C07K14/4716 , C07K14/705 , C12N7/00 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14151
摘要： The present invention provides methods of achieving directed evolution of viruses by in vivo screening or “panning” to identify viruses comprising scrambled AAV capsids having characteristics of interest, e.g., tropism profile and/or neutralization profile (e.g., ability to evade neutralizing antibodies). The invention also provides scrambled AAV capsids and virus particles comprising the same.
摘要翻译：本发明提供了通过体内筛选或“平移”来鉴定包含感兴趣特征的加扰的AAV衣壳的病毒（例如向性分布和/或中和特征（例如逃避中和抗体的能力））来实现病毒定向进化的方法。本发明还提供加扰的AAV衣壳和包含其的病毒颗粒。

9. 发明申请

US20090004743A1 HELPER VIRUS-FREE AAV PRODUCTION 有权
标题翻译：帮助病毒免费AAV生产
公开(公告)号：US20090004743A1
公开(公告)日：2009-01-01
申请号：US12140820
申请日：2008-06-17
申请人： Forrest K. Ferrari , Xiao Xiao , Richard Jude Samulski
发明人： Forrest K. Ferrari , Xiao Xiao , Richard Jude Samulski
IPC分类号： C12N15/87 , C12N7/00 , C12N5/06
CPC分类号： C12N7/00 , C07K14/005 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2750/14143 , C12N2750/14152
摘要： A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
摘要翻译：描述了生产基本上不含污染辅助病毒的腺相关病毒种群和重组腺相关病毒种群的方法。该方法利用辅助病毒载体的转染来代替常规方法中使用的辅助病毒感染。

10. 发明授权

US06548286B1 Methods for increasing the efficiency of recombinant AAV product 失效
标题翻译：提高重组AAV产品效率的方法
公开(公告)号：US06548286B1
公开(公告)日：2003-04-15
申请号：US09060102
申请日：1998-04-14
申请人： Richard Jude Samulski , Xiao Xiao , Richard Snyder
发明人： Richard Jude Samulski , Xiao Xiao , Richard Snyder
IPC分类号： C12N701
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C12N7/00 , C12N2710/10322 , C12N2710/10343 , C12N2750/14121 , C12N2750/14122 , C12N2750/14143 , C12N2750/14152
摘要： The present invention relates to methods and compositions for increasing the production of high titre stocks of recombinant AAV (rAAV) through regulation of expression of the AAV REP and CAP proteins. The methods and compositions of the invention are based on the observation that the low level expression of the AAV REP protein increases the production of AAV viral capsid protein and efficiency of packaging resulting in production of higher titre recombinant viral stocks. The invention encompasses recombinant AAV vectors that direct the expression of AAV REP and CAP proteins and the use of such vectors for the production of novel stable cell lines capable of generating high titre rAAV vectors. The invention provides methods for regulating the expression of the AAV REP gene at the transcriptional and post-translational level. The methods and compositions of the invention can be used to produce high titre stocks of rAAV which can be used in gene therapy for the purpose of transferring genetic information into appropriate host cells for the management and correction of human diseases including inherited and acquired disorders.
摘要翻译：本发明涉及通过调节AAV REP和CAP蛋白的表达来增加重组AAV（rAAV）的高滴度储备的生产的方法和组合物。本发明的方法和组合基于AAV REP蛋白的低水平表达增加AAV病毒衣壳蛋白的产生和包装效率的观察，导致产生较高滴度的重组病毒种群。本发明包括引导AAV REP和CAP蛋白的表达的重组AAV载体，以及这些载体用于产生能产生高滴度rAAV载体的新型稳定细胞系的用途。本发明提供了在转录和翻译后水平调节AAV REP基因表达的方法。本发明的方法和组合物可用于生产高滴度的rAAV，其可用于基因治疗，以将遗传信息转移到合适的宿主细胞中，用于管理和矫正包括遗传和获得性疾病在内的人类疾病。

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