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1. 发明授权

US07622638B2 Genetic manipulation of condensed tannins 有权
标题翻译：浓缩单宁的遗传操作
公开(公告)号：US07622638B2
公开(公告)日：2009-11-24
申请号：US10610351
申请日：2003-06-30
申请人： Richard A. Dixon , Nancy L. Paiva , Deyu Xie , Shashi Sharma
发明人： Richard A. Dixon , Nancy L. Paiva , Deyu Xie , Shashi Sharma
IPC分类号： A01H5/00 , A01H5/10 , A01H1/00 , C12N15/82 , C12N15/53
CPC分类号： C12N9/0006 , C12N15/8243 , C12N15/825
摘要： The invention provides method and compositions for the modulation of condensed tannin production in plants. The methods of the invention allow creation of plants having novel phenotypes. Increased expression of condensed tannins in plants may be used to increase the nutritional value of food plants for both human and animal consumption. Increased condensed tannin content also reduces the potential for bloat in animals fed certain forage plants low in condensed tannin content. The invention may also be used to modify plant pigmentation.
摘要翻译：本发明提供用于调节植物中缩合单宁生产的方法和组合物。本发明的方法允许产生具有新表型的植物。植物中浓缩单宁的增加表达可用于增加食用植物对人和动物消费的营养价值。浓缩单宁含量的增加也降低了饲喂低浓缩单宁含量的某些牧草植物的动物的膨胀潜力。本发明也可用于修饰植物色素沉着。

2. 发明授权

US06846971B1 Regeneration and genetic transformation of Acacia mangium 有权
标题翻译：相思树的再生和遗传转化
公开(公告)号：US06846971B1
公开(公告)日：2005-01-25
申请号：US09936612
申请日：2000-01-19
申请人： Deyu Xie , Yan Hong
发明人： Deyu Xie , Yan Hong
IPC分类号： A01H1/00 , A01H4/00 , C12N15/82 , C12N15/84 , C12N5/04 , C12N5/10
CPC分类号： C12N15/8205 , A01H4/001 , A01H4/008
摘要： The present invention is directed to a method of Acacia mangium regeneration through organogenesis and a method of genetic transformation of Acacia mangium. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced to Acacia mangium via Agrobacterium infection. GUS staining in the regenerated plants and Southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene.
摘要翻译：本发明涉及通过器官发生的相思树再生的方法和相思树的遗传转化方法。再生方法包括从幼苗和营养微繁殖苗的不同部位诱导愈伤组织为外植体; 不定芽诱导，然后是羽状叶和芽伸长，最后将细长的芽诱导到根部。基于再生系统，通过农杆菌感染将花椰菜花叶病毒启动子下的标记基因GUS引入相思树。再生植物中的GUS染色和Southern印迹杂交证明外源基因并入宿主基因组和外源基因的表达。

3. 发明授权

US07368631B2 Regeneration and genetic transformation of Acacia mangium 有权
标题翻译：相思树的再生和遗传转化
公开(公告)号：US07368631B2
公开(公告)日：2008-05-06
申请号：US11007194
申请日：2004-12-09
申请人： Deyu Xie , Yan Hong
发明人： Deyu Xie , Yan Hong
IPC分类号： C12N5/04 , C12N5/10 , C12N15/82 , C12N15/84
CPC分类号： C12N15/8205 , A01H4/001 , A01H4/008
摘要： The present invention is directed to a method of Acacia mangium regeneration through organogenesis and a method of genetic transformation of Acacia mangium. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced to Acacia mangium via Agrobacterium infection. GUS staining in the regenerated plants and Southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene.
摘要翻译：本发明涉及通过器官发生的相思树再生的方法和相思树的遗传转化方法。再生方法包括从幼苗和营养微繁殖苗的不同部位诱导愈伤组织为外植体; 不定芽诱导，然后是羽状叶和芽伸长，最后将细长的芽诱导到根部。基于再生系统，通过农杆菌感染将花椰菜花叶病毒启动子下的标记基因GUS引入相思树。再生植物中的GUS染色和Southern印迹杂交证明外源基因并入宿主基因组和外源基因的表达。

4. 发明申请

US20050155116A1 Regeneration and genetic transformation of acacia mangium 有权
标题翻译：金合欢的再生和遗传转化
公开(公告)号：US20050155116A1
公开(公告)日：2005-07-14
申请号：US11007194
申请日：2004-12-09
申请人： Deyu Xie , Yan Hong
发明人： Deyu Xie , Yan Hong
IPC分类号： A01H1/00 , A01H4/00 , C12N15/82 , C12N15/84
CPC分类号： C12N15/8205 , A01H4/001 , A01H4/008
摘要： The present invention is directed to a method of Acacia mangium regeneration through organogenesis and a method of genetic transformation of Acacia mangium. The method of regeneration comprises inducing callus from different parts of seedlings and vegetatively micropropagated plantlets as explants; adventitious bud induction followed by pinnate leaf and bud elongation and eventually, elongated shoots were induced to root. Based on the regeneration system, a marker gene GUS under cauliflower mosaic virus promoter was introduced to Acacia mangium via Agrobacterium infection. GUS staining in the regenerated plants and Southern blot hybridization prove the incorporation of the foreign gene into the host genome and expression of the foreign gene.
摘要翻译：本发明涉及通过器官发生的相思树再生的方法和相思树的遗传转化方法。再生方法包括从幼苗和营养微繁殖苗的不同部位诱导愈伤组织为外植体; 不定芽诱导，然后是羽状叶和芽伸长，最后将细长的芽诱导到根部。基于再生系统，通过农杆菌感染将花椰菜花叶病毒启动子下的标记基因GUS引入相思树。再生植物中的GUS染色和Southern印迹杂交证明外源基因并入宿主基因组和外源基因的表达。

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