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    • 4. 发明申请
    • Magnetic particle tagged reagents and techniques
    • 磁性颗粒标记的试剂和技术
    • US20070172899A1
    • 2007-07-26
    • US11715411
    • 2007-03-08
    • Henry GrahamJohn GormanJames Rowell
    • Henry GrahamJohn GormanJames Rowell
    • G01N33/567G01N33/553
    • G01N33/80G01N2446/00
    • Methods for separating, in a continuous, multizone fluid medium, cells, particles, or other molecules of interest (MOI) from associated or contaminating unwanted materials not of interest (MNOI). The invention involves forced movement of MOI into certain zones having properties which deter the entry of unwanted materials. Differential movement of MOI and MNOI occurs by active counterforces that move MNOI but not MOI. MOI are tagged with magnetic particles and moved with a magnetic field through a fluid, or zones, of higher specific gravity that prevents, by flotation counterforce, unwanted less dense materials from entering. Surfaces specifically coated with reactants are reactive with the MOI in the tagged magnetic particle complex and of buoyant or other forces are used to remove any unbound material from the surface before reading. Readable labels, in addition to the magnetic particle tagged complex itself, such as enzymes, fluorophors, chemiluminescent materials, radioactive isotopes, chromogenic and fluorogenic substrates and other labels may be used. In most embodiments, materials of interest are delivered to a special final zone for reading or harvesting. The invention applies to many assays, diagnostic tests, separative procedures and chemical syntheses.
    • 在连续的多区流体培养基中分离来自相关或污染的不需要的物质(MNOI)的细胞,颗粒或其他感兴趣的分子(MOI)的方法。 本发明涉及将MOI强制移动到具有阻止不想要的材料进入的特性的某些区域中。 MOI和MNOI的差异运动是通过移动MNOI而不是MOI的主动抵制来实现的。 MOI用磁性颗粒标记,并通过具有较高比重的流体或区域的磁场移动,通过浮选反作用力阻止不需要的较不致密的材料进入。 特别涂覆有反应物的表面与标记的磁性颗粒复合体中的MOI反应,并且在读取之前使用浮力或其它力从表面去除任何未结合的材料。 可以使用除了磁性颗粒标记的复合体本身之外的可读标签,例如酶,荧光体,化学发光材料,放射性同位素,显色和荧光底物等标记。 在大多数实施例中,感兴趣的材料被输送到用于阅读或收获的特殊最后区域。 本发明适用于许多测定,诊断测试,分离程序和化学合成。
    • 5. 发明授权
    • Method and apparatus for coagulation based assays
    • 用于基于凝固的测定法
    • US06451610B1
    • 2002-09-17
    • US09291776
    • 1999-04-14
    • Anne Jessica GormanRobert SamoJohn Gorman
    • Anne Jessica GormanRobert SamoJohn Gorman
    • G01N3386
    • G01N33/5302G01N33/86
    • An activated partial thromboplastin time (APTT) test is described which employs fresh, whole blood that has not been anticoagulated. The method employs a disposable cuvette having specific reagents placed therein. A sample of whole blood is introduced into the cuvette which is drawn into at least one conduit by a pneumatic pump. Initially, the calcium in the sample is chelated at a first reaction site in the conduit, and thereafter is moved through the conduit to a second reaction site where the sample is coagulated by an APTT reagent. After a predetermined incubation time, the sample is moved to a third reaction site where it is recalcified. Clotting time is measured from the time that the sample is recalcified to the time that clot formation is visibly detected.
    • 描述了激活的部分凝血活酶时间(APTT)测试,其使用尚未抗凝的新鲜全血。 该方法采用在其中放置特定试剂的一次性比色皿。 将全血样品引入比色皿中,该比色皿通过气动泵吸入至少一个导管中。 最初,样品中的钙在导管中的第一个反应部位被螯合,然后通过导管移动到第二个反应部位,在该第二个反应部位,样品被APTT试剂凝结。 在预定的孵育时间之后,将样品移至第三反应部位,在此其再次被煅烧。 凝固时间是从样品再次煅烧的时间到可见的凝块形成时间来测量的。
    • 7. 发明申请
    • Magnetic particle tagged blood bank reagents and techniques
    • 磁颗粒标记血库试剂和技术
    • US20070059782A1
    • 2007-03-15
    • US11518189
    • 2006-09-11
    • Henry GrahamJohn GormanJames Rowell
    • Henry GrahamJohn GormanJames Rowell
    • G01N33/567G01N33/551
    • G01N33/80G01N2446/00
    • Magnets and magnetic particle-labeled reagents are used to capture and/or release magnetic particle-tagged entities for immunohematology diagnostic testing purposes, especially tests performed in blood banking. The magnetic tagged entities may be tagged antibodies, tagged blood cells, tagged universal binding partners, especially tagged lectins and tagged Coombs reagent, and other binding agents such as biotin-avidin, Protein A or G, ligands and their receptors and the like. Separation of unbound material from bound material is effected through the use of one or both the magnetic field effect on the magnetic labeled reactants and the density gradients of layers of an assay construct. Constructs such as chromatographic strip lateral flow format, and liquid phase reactions in suitable vessels with end point determinations that do not require centrifugation to detect reacted entities. Readable labels such as enzymes, fluorophors, chemiluminescent materials, radioactive isotopes, and other labels may be attached to Coombs reagent to provide a readable product of the Coombs reagent with any antibody participating in the assay.
    • 使用磁铁和磁性颗粒标记的试剂来捕获和/或释放用于免疫血液学诊断测试目的的磁性颗粒标记的实体,特别是在血库中进行的测试。 磁性标签实体可以是标记抗体,标记血细胞,标记的通用结合配偶体,特别是标记的凝集素和标记的库姆斯试剂,以及其它结合剂,例如生物素 - 抗生物素蛋白,蛋白A或G,配体及其受体等。 未结合材料与结合材料的分离通过使用对磁性标记的反应物的磁场效应和测定构建体的层的密度梯度中的一种或两种来实现。 构建如色谱条横向流动形式和液相反应在合适的血管,终点测定不需要离心检测反应的实体。 可将可读标签如酶,荧光体,化学发光材料,放射性同位素和其他标记连接到Coombs试剂上,以提供Coombs试剂与参与测定的任何抗体的可读产物。
    • 10. 发明申请
    • METHOD, SYSTEM AND APPARATUS FOR AN EFFICIENT DESIGN AND OPERATION OF A PUMP MOTOR
    • 一种泵电机高效设计和运行的方法,系统和设备
    • US20090151801A1
    • 2009-06-18
    • US12333494
    • 2008-12-12
    • John GormanWilliam LizaldeRobert Kulakowski
    • John GormanWilliam LizaldeRobert Kulakowski
    • G05D7/06H02P7/00
    • F04D15/0066F04D13/14Y10T137/85954Y10T137/85986Y10T137/86027Y10T137/86131
    • A method of operating a controller for a pool pump motor includes the steps of setting an operating mode for the pool pump motor, setting a torque value for the pool pump motor corresponding to the operating mode, setting a high operating threshold and a low operating threshold corresponding to the torque value, operating the pool pump motor in a constant torque mode using the torque value, monitoring an operating parameter of the pool pump motor corresponding to a load on the pool pump motor, discontinuing operating the pool pump motor when the operating parameter is higher than the high operating threshold or lower than the low operating threshold and signaling a pool pump motor fault upon the discontinuing operating the pool pump motor. The operating parameter of the pool pump motor is RPM or current supplied to the pool pump motor. Additionally disclosed are energy efficient pool hydraulic design techniques that increase the energy efficiency of hydraulic systems.
    • 一种操作游泳池泵马达的控制器的方法包括以下步骤:设置池泵马达的运行模式,根据运行模式设定池泵马达的转矩值,设定高运行阈值和低运行阈值 对应于扭矩值,使用扭矩值在恒定扭矩模式下操作水池泵电动机,监测与水泵电动机上的负载相对应的水池泵电动机的操作参数,当操作参数 高于高运行阈值或低于低运行阈值,并在停止运行池泵电机时发出池泵电机故障信号。 水泵电机的运行参数为RPM或电流供给水泵电机。 另外公开了提高液压系统的能量效率的节能池液压设计技术。