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1. 发明授权

US06183755B2 Active proteins from Borrelia burgdorferi 失效
标题翻译：来自博氏疏螺旋体的活性蛋白
公开(公告)号：US06183755B2
公开(公告)日：2001-02-06
申请号：US09196293
申请日：1998-11-19
申请人： Manfred Motz , Erwin Soutscheck , Renate Fuchs , Bettina Wilske , Vera Preac-Mursic
发明人： Manfred Motz , Erwin Soutscheck , Renate Fuchs , Bettina Wilske , Vera Preac-Mursic
IPC分类号： A61K3902
CPC分类号： C07K16/1207 , A61K38/00 , A61K39/00 , C07K14/20 , G01N33/56911 , Y02A50/401 , Y02A50/57 , Y10S436/804 , Y10S530/825
摘要： Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. The Borrelia burgdorferi-specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).
摘要翻译：通过遗传操作在微生物中制备了来自博氏疏螺旋体的各种免疫活性蛋白质。为此，使用合适的筛选方法从布氏疏螺旋体基因库中选择特异性DNA序列，或者通过使用选择的杂交探针的DNA扩增直接制备，并置于诱导型启动子的控制下，例如， lac启动子。由于对表达的抗原的有效纯化方法的描述，可以以合适的方式提供蛋白质是可能的。这些蛋白质可用于制备特异性敏感的诊断测定试剂盒。免疫活性蛋白质的特异性组合使得精确的诊断成为可能。此外，已经产生了单克隆抗体，并且用作用于在测试样品中或培养后直接检测病原体的试剂。疏螺旋体异常特异性DNA序列可用于直接检测患者样品中的病原体（例如通过PCR反应）。

2. 发明授权

US07083792B2 Immunologically active proteins from Borrelia burgdorferi 失效
公开(公告)号：US07083792B2
公开(公告)日：2006-08-01
申请号：US10762665
申请日：2004-01-22
申请人： Renate Fuchs , Bettina Wilske , Vera Preac-Mursic , Manfred Motz , Erwin Soutscheck
发明人： Renate Fuchs , Bettina Wilske , Vera Preac-Mursic , Manfred Motz , Erwin Soutscheck
IPC分类号： A61K39/00 , A61K39/02 , C07K1/00 , C07K14/00
CPC分类号： C07K16/1207 , A61K38/00 , A61K39/00 , C07K14/20 , G01N33/56911 , Y02A50/401 , Y02A50/57 , Y10S436/804 , Y10S530/825
摘要： Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. The Borrelia burgdorferi-specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).

3. 发明授权

US06509019B1 Immunologically active proteins from Borrelia burgdorferi 失效
标题翻译：来自博氏疏螺旋体的免疫活性蛋白
公开(公告)号：US06509019B1
公开(公告)日：2003-01-21
申请号：US09711546
申请日：2000-11-13
申请人： Renate Fuchs , Bettina Wilske , Vera Preac-Mursic , Manfred Motz , Erwin Soutscheck
发明人： Renate Fuchs , Bettina Wilske , Vera Preac-Mursic , Manfred Motz , Erwin Soutscheck
IPC分类号： A61K3902
CPC分类号： C07K16/1207 , A61K38/00 , A61K39/00 , C07K14/20 , G01N33/56911 , Y02A50/401 , Y02A50/57 , Y10S436/804 , Y10S530/825
摘要： Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. The Borrelia burgdorferi-specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).
摘要翻译：通过遗传操作在微生物中制备了来自博氏疏螺旋体的各种免疫活性蛋白质。为此，使用合适的筛选方法从布氏疏螺旋体基因库中选择特异性DNA序列，或者通过使用选择的杂交探针的DNA扩增直接制备，并置于诱导型启动子的控制下，例如， lac启动子。由于对表达的抗原的有效纯化方法的描述，可以以合适的方式提供蛋白质是可能的。这些蛋白质可用于制备特异性敏感的诊断测定试剂盒。免疫活性蛋白质的特异性组合使得精确的诊断成为可能。此外，已经产生了单克隆抗体，并且用作用于在测试样品中或培养后直接检测病原体的试剂。疏螺旋体异常特异性DNA序列可用于直接检测患者样品中的病原体（例如通过PCR反应）。

4. 发明授权

US06753183B2 Immunologically active proteins from Borrelia burgdorferi 失效
标题翻译：来自博氏疏螺旋体的免疫活性蛋白
公开(公告)号：US06753183B2
公开(公告)日：2004-06-22
申请号：US10289795
申请日：2002-11-07
申请人： Renate Fuchs , Bettina Wilske , Vera Preac-Mursic , Manfred Motz , Erwin Soutscheck
发明人： Renate Fuchs , Bettina Wilske , Vera Preac-Mursic , Manfred Motz , Erwin Soutscheck
IPC分类号： G01N3353
CPC分类号： C07K16/1207 , A61K38/00 , A61K39/00 , C07K14/20 , G01N33/56911 , Y02A50/401 , Y02A50/57 , Y10S436/804 , Y10S530/825
摘要： Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. The Borrelia burgdorferi—specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).
摘要翻译：通过遗传操作在微生物中制备了来自博氏疏螺旋体的各种免疫活性蛋白质。为此，使用合适的筛选方法从布氏疏螺旋体基因库中选择特异性DNA序列，或者通过使用选择的杂交探针的DNA扩增直接制备，并置于诱导型启动子的控制下，例如， lac启动子。由于对表达的抗原的有效纯化方法的描述，可以以合适的方式提供蛋白质是可能的。这些蛋白质可用于制备特异性敏感的诊断测定试剂盒。免疫活性蛋白质的特异性组合使得精确的诊断成为可能。此外，已经产生了单克隆抗体，并且用作用于在测试样品中或培养后直接检测病原体的试剂。疏螺旋体异常特异性DNA序列可用于直接检测患者样品中的病原体（例如通过PCR反应）。

5. 发明授权

US06248538B1 Immunologically active PC proteins from Borrelia burgdorferi 失效
标题翻译：来自博氏疏螺旋体的免疫活性PC蛋白
公开(公告)号：US06248538B1
公开(公告)日：2001-06-19
申请号：US08209603
申请日：1994-03-10
申请人： Manfred Motz , Erwin Soutscheck , Renate Fuchs , Bettina Wilske , Vera Preac-Mursic
发明人： Manfred Motz , Erwin Soutscheck , Renate Fuchs , Bettina Wilske , Vera Preac-Mursic
IPC分类号： G01N3353
CPC分类号： C07K16/1207 , A61K38/00 , A61K39/00 , C07K14/20 , G01N33/56911 , Y02A50/401 , Y02A50/57 , Y10S436/804 , Y10S530/825
摘要： Various immunologically active proteins from Borrelia burgdorferi have been prepared by genetic manipulation in microorganisms. To do this, the specific DNA sequences were selected from a B. burgdorferi gene bank using suitable screening methods, or were prepared directly by DNA amplification using selected hybridization probes, and were placed under the control of inducible promoters such as, for example, the lac promoter. It has been possible, owing to description of efficient purification methods for the expressed antigens, to provide the proteins in a suitable way. These proteins can be used to produce specific and sensitive diagnostic assay kits. The specific combination of the immunologically active proteins makes precise diagnosis possible. Furthermore, monoclonal antibodies have been generated and are used as reagents for detecting pathogens directly in test samples or after cultivation. The Borrelia burgdorferi-specific DNA sequences can be employed for direct detection of the pathogen in patients' samples (for example by means of the PCR reaction).
摘要翻译：通过遗传操作在微生物中制备了来自博氏疏螺旋体的各种免疫活性蛋白质。为此，使用合适的筛选方法从布氏疏螺旋体基因库中选择特异性DNA序列，或者通过使用选择的杂交探针的DNA扩增直接制备，并置于诱导型启动子的控制下，例如， lac启动子。由于对表达的抗原的有效纯化方法的描述，可以以合适的方式提供蛋白质是可能的。这些蛋白质可用于制备特异性敏感的诊断测定试剂盒。免疫活性蛋白质的特异性组合使得精确的诊断成为可能。此外，已经产生了单克隆抗体，并且用作用于在测试样品中或培养后直接检测病原体的试剂。疏螺旋体异常特异性DNA序列可用于直接检测患者样品中的病原体（例如通过PCR反应）。

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