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    • 2. 发明授权
    • Rapid one-step reverse transcriptase PCR
    • 快速一步逆转录酶PCR
    • US08119353B2
    • 2012-02-21
    • US12061700
    • 2008-04-03
    • Waltraud AnkenbauerUrsula GreplRita Haerteis
    • Waltraud AnkenbauerUrsula GreplRita Haerteis
    • C12Q1/68C12P19/34
    • C12Q1/6846C12Q2527/113C12Q2521/107
    • The present invention is directed to a method for performing a one-step RT-PCR for amplifying a target RNA comprising the steps (i) providing a sample which is supposed to contain said target RNA (ii) adding a reaction mixture comprising all reagents necessary to reverse transcribe said target RNA into cDNA and amplify at least a portion of said cDNA (iii) incubating said sample for a time interval of 0 seconds to 40 seconds at a temperature between 20° C. and 65° C., and (iv) subjecting said sample to multiple cycles of a thermocycling protocol wherein the temperature of said sample is varied between at least a first temperature between 37° C. and 72° C. and a second temperature between 85° C. and 100° C.
    • 本发明涉及一种用于进行扩增靶RNA的一步RT-PCR的方法,包括步骤(i)提供假定含有所述靶RNA的样品(ii)加入包含所需试剂的反应混合物 将所述靶RNA逆转录入cDNA并扩增所述cDNA的至少一部分(iii)在20℃至65℃的温度下将所述样品温育0秒至40秒的时间间隔,和(iv )使所述样品经受热循环方案的多个循环,其中所述样品的温度在37℃至72℃的至少第一温度和85℃至100℃的第二温度之间变化。