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1. 发明授权

US08859199B2 Use of an extraction control in a method of extracting nucleic acids 有权
标题翻译：在提取核酸的方法中使用提取控制
公开(公告)号：US08859199B2
公开(公告)日：2014-10-14
申请号：US12062602
申请日：2008-04-04
申请人： Tobin Hellyer , Thomas Fort , Ray A. McMillian
发明人： Tobin Hellyer , Thomas Fort , Ray A. McMillian
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/00 , C12N15/00
CPC分类号： C12Q1/6806 , C12Q1/689 , C12Q2545/114 , C12Q2531/113 , C12Q2545/101
摘要： The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.
摘要翻译：本发明涉及确保来自核酸生物样品的提取处理的有效性的方法。本发明的方法能够区分来自样品的核酸提取的可能缺陷以及随后扩增步骤中的可能缺陷。本发明还涉及用于从生物样品中提取核酸的包装阵列。

2. 发明授权

US08658397B2 Assay for detecting closely-related serotypes of human papillomavirus (HPV) 有权
标题翻译：用于检测人乳头状瘤病毒（HPV）密切相关血清型的测定
公开(公告)号：US08658397B2
公开(公告)日：2014-02-25
申请号：US13028589
申请日：2011-02-16
申请人： Chi Chen , Michael Porter , Gregory A. Richart , Ray A. McMillian , Dolores C. Peck
发明人： Chi Chen , Hugh J. Peck , Michael Porter , Gregory A. Richart , Ray A. McMillian
IPC分类号： C12P19/34
CPC分类号： C12Q1/708
摘要： A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.
摘要翻译：用于检测人乳头状瘤病毒（HPV）的多种血清型的实时Taq-Man PCR测定法，其中检测的血清型数量超过用于检测的比色通道的数量。将生物样品与三种寡核苷酸引物/探针组合，使得探针和引物与靶序列退火。每个引物/探针组对于生物的特定血清型至少是优选的。第一和第二引物/探针组相对于彼此退化。第三引物/探针组相对于第一和第二引物/探针组不退化，并鉴别第三血清型。第三引物/探针组具有在与简并引物/探针组探针的信号部分发射的波长相同或不同的波长处发射信号的信号部分。靶序列（如果存在）被扩增和检测。

3. 发明授权

US07371531B2 Use of an extraction control in a method of extracting nucleic acids 有权
标题翻译：在提取核酸的方法中使用提取控制
公开(公告)号：US07371531B2
公开(公告)日：2008-05-13
申请号：US11114952
申请日：2005-04-25
申请人： Tobin Hellyer , Thomas Fort , Ray A. McMillian
发明人： Tobin Hellyer , Thomas Fort , Ray A. McMillian
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6806 , C12Q1/689 , C12Q2545/114 , C12Q2531/113 , C12Q2545/101
摘要： The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.
摘要翻译：本发明涉及确保来自核酸生物样品的提取处理的有效性的方法。本发明的方法能够区分来自样品的核酸提取的可能缺陷以及随后扩增步骤中的可能缺陷。本发明还涉及用于从生物样品中提取核酸的包装阵列。

4. 发明授权

US5447848A Detection of campylobacter with nucleic acid probes 失效
标题翻译：用核酸探针检测弯曲杆菌
公开(公告)号：US5447848A
公开(公告)日：1995-09-05
申请号：US216679
申请日：1988-07-07
申请人： Susan M. Barns , Ray A. McMillian , David J. Lane , Mark L. Collins , James E. Awell , Ayoub Rashtchian
发明人： Susan M. Barns , Ray A. McMillian , David J. Lane , Mark L. Collins , James E. Awell , Ayoub Rashtchian
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N15/00 , C12Q1/04 , C12Q1/68 , C12R1/01 , G01N33/50 , C07H21/00 , C12Q1/02
CPC分类号： C12Q1/6813 , C12Q1/689
摘要： Nucleic acid probes capable of specifically hybridizing to rRNA of Campylobacter jejuni, C. coli and C. laridis and not to rRNA or rRNA genes of Pseudomonas aeuroginosa, E. coli or Salmonella typhimunium are described along with methods utilizing such probes for the detection of Campylobacter in clinical, food and other samples.
摘要翻译：与能够与空肠弯曲杆菌，大肠杆菌和C.laridis的rRNA特异性杂交的核酸探针以及不属于恶臭假单胞菌（Pseudomonas aeuroginosa），大肠杆菌或鼠伤寒沙门氏菌的rRNA或rRNA基因的核酸探针与使用这种探针用于检测弯曲杆菌在临床，食品等样品中。

5. 发明授权

US09777336B2 Assay for Chlamydia trachomatis by amplification and detection of Chlamydia trachomatis cytotoxin gene 有权
公开(公告)号：US09777336B2
公开(公告)日：2017-10-03
申请号：US12583832
申请日：2009-08-26
申请人： Courtney E. Maus , Ray A. McMillian
发明人： Courtney E. Maus , Ray A. McMillian
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： A region of the Chlamydia trachomatis cytotoxin gene has been identified which is useful for performing amplification assays to determine specifically whether C. trachomatis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of C. trachomatis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.

6. 发明授权

US6162605A Amplification and detection of shiga-like toxin I producing organisms 失效
标题翻译：扩增和检测志贺样毒素I产生菌
公开(公告)号：US6162605A
公开(公告)日：2000-12-19
申请号：US289803
申请日：1999-04-12
申请人： Thomas L. Fort , Tobin J. Hellyer , Ray A. McMillian , Qimin You
发明人： Thomas L. Fort , Tobin J. Hellyer , Ray A. McMillian , Qimin You
IPC分类号： G01N33/53 , C12M1/00 , C12N15/09 , C12Q1/04 , C12Q1/68 , G01N1/10 , G01N33/566 , G01N33/569 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/689
摘要： Amplification primers and methods for specific amplification and detection of a Shiga-like toxin I (SLT-I) target are disclosed. The primer-target binding sequences are useful for amplification and detection of SLT-I target in a variety of amplification and detection reactions.
摘要翻译：公开了用于特异性扩增和检测志贺样毒素I（SLT-1）靶的扩增引物和方法。引物 - 靶结合序列可用于扩增和检测各种扩增和检测反应中的SLT-1靶。

7. 发明授权

US06060252A Amplification and detection of shigella spp. and enteroinvasive strains of Escherichia coli 失效
标题翻译：志贺菌属的扩增和检测和大肠埃希氏菌肠毒素菌株
公开(公告)号：US06060252A
公开(公告)日：2000-05-09
申请号：US289750
申请日：1999-04-12
申请人： Tobin J. Hellyer , Ray A. McMillian
发明人： Tobin J. Hellyer , Ray A. McMillian
IPC分类号： G01N33/53 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/04 , C12Q1/68 , C12R1/01 , G01N1/10 , G01N27/416 , A61K49/00 , C07H19/00 , C07H21/02 , C12P19/34
CPC分类号： C12Q1/689
摘要： Amplification primers and methods for specific amplification and detection of a Shigella spp. and enteroinvasive strains of Escherichia coli (EIEC) target are disclosed. The primer-target binding sequences are useful for amplification and detection of Shigella and EIEC target in a variety of amplification and detection reactions.
摘要翻译：扩增引物和用于特异性扩增和检测志贺氏菌属的方法。和肠道侵袭性大肠杆菌菌株（EIEC）靶标。引物 - 靶结合序列可用于扩增和检测各种扩增和检测反应中的志贺氏菌和EIEC靶。

8. 发明授权

US5663049A Detection of campylobacter 失效
标题翻译：检测弯曲杆菌
公开(公告)号：US5663049A
公开(公告)日：1997-09-02
申请号：US453756
申请日：1995-05-30
申请人： Susan M. Barns , Ray A. McMillian , David J. Lane , Mark L. Collins , James E. Awell , Ayoub Rashtchian
发明人： Susan M. Barns , Ray A. McMillian , David J. Lane , Mark L. Collins , James E. Awell , Ayoub Rashtchian
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N15/00 , C12Q1/04 , C12Q1/68 , C12R1/01 , G01N33/50 , C07H21/02
CPC分类号： C12Q1/6813 , C12Q1/689
摘要： The invention relates to methods of using nucleic acid probes capable of specifically hybridizing to rRNA of Campylobacter jejuni, C. coli and C. laridis and not to rRNA or rRNA genes of Pseudomonas aeuroginosa, E. coli or Salmonella typhimunium for the detection of Campylobacter in clinical, food and other samples.
摘要翻译：本发明涉及使用能够与空肠弯曲杆菌，大肠杆菌和C.laridis的rRNA特异性杂交的核酸探针的方法，而不涉及用于检测弯曲杆菌的弯曲杆菌的假单胞菌（Pseudomonas aeuroginosa），大肠杆菌（Salmonella typhimunium）的rRNA或rRNA基因临床，食品等样品。

9. 发明申请

US20090149337A1 Use of an Extraction Control in a Method of Extracting Nucleic Acids 有权
标题翻译：在提取核酸的方法中使用提取控制
公开(公告)号：US20090149337A1
公开(公告)日：2009-06-11
申请号：US12062602
申请日：2008-04-04
申请人： Tobin Hellyer , Thomas Fort , Ray A. McMillian , Matthew P. Collis , Thomas L. Fort
发明人： Tobin Hellyer , Thomas Fort , Ray A. McMillian , Matthew P. Collis , Thomas L. Fort
IPC分类号： C40B30/04 , C40B40/08
CPC分类号： C12Q1/6806 , C12Q1/689 , C12Q2545/114 , C12Q2531/113 , C12Q2545/101
摘要： The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.
摘要翻译：本发明涉及确保来自核酸生物样品的提取处理的有效性的方法。本发明的方法能够区分来自样品的核酸提取的可能缺陷以及随后扩增步骤中的可能缺陷。本发明还涉及用于从生物样品中提取核酸的包装阵列。

10. 发明授权

US06258546B1 Detection of nucleic acid amplification 有权
标题翻译：检测核酸扩增
公开(公告)号：US06258546B1
公开(公告)日：2001-07-10
申请号：US09602996
申请日：2000-06-23
申请人： Ray A. McMillian , Karen Eckert , Donald W. Copertino , Tobin J. Hellyer
发明人： Ray A. McMillian , Karen Eckert , Donald W. Copertino , Tobin J. Hellyer
IPC分类号： C12Q168
CPC分类号： C12Q1/6818 , C12Q1/6844 , C12Q2531/119 , C12Q2525/301 , C12Q2521/301 , C12Q2565/1025
摘要： For use in nucleic acid amplification reactions, the detector oligonucleotides of the invention comprise a target binding sequence which is at least partially the same as the target binding sequence of an amplification primer present in the target amplification reaction, so that the detector oligonucleotide and the amplification primer compete for hybridization to the same sequence in the target. Hybridization of the amplification primer to the target upstream from the detector oligonucleotide generates a nickable restriction endonuclease recognition site. When this site is nicked and strand displacement occurs from the nick, both the 3′ end of the amplification primer and the detector oligonucleotide are displaced. The displaced detector oligonucleotide may then be detected as an indication of the presence of the target sequence, for example by unfolding of a fluorescently labeled secondary structure present in the detector oligonucleotide to reduce fluorescence quenching.
摘要翻译：为了用于核酸扩增反应，本发明的检测寡核苷酸包含与目标扩增反应中存在的扩增引物的靶结合序列至少部分相同的靶结合序列，使得检测寡核苷酸和扩增引物竞争与目标中相同序列的杂交。扩增引物与检测寡核苷酸上游的靶的杂交产生可切口的限制性内切核酸酶识别位点。当该位点被切口并且从切口发生链置换时，扩增引物的3'末端和检测器寡核苷酸都被置换。然后可以将位移的检测器寡核苷酸作为目标序列存在的指示进行检测，例如通过解折叠存在于检测器寡核苷酸中的荧光标记的二级结构以减少荧光猝灭。

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