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1. 发明授权

US08098374B2 Highly sensitive spectroscopic unit 有权
标题翻译：高灵敏度光谱单位
公开(公告)号：US08098374B2
公开(公告)日：2012-01-17
申请号：US12444244
申请日：2007-10-02
申请人： Ralf Wolleschensky , Hans-Juergen Dobschal , Reinhard Steiner
发明人： Ralf Wolleschensky , Hans-Juergen Dobschal , Reinhard Steiner
IPC分类号： G01J3/28
CPC分类号： G01J3/1804 , G01J3/02 , G01J3/021 , G01J3/0291 , G01J3/14 , G01J3/26 , G01J3/2803
摘要： The invention is directed to a highly sensitive spectrum analysis unit with a diffraction grating, wherein a parallel light bundle having a wavelength range impinges on a diffraction grating which splits the different wavelengths into spectra by diffraction in first directions, and wavelength partial ranges of the spectrally split light bundle can be focused on a detector row by means of camera optics, and evaluation electronics are connected to the detector row and acquire the generated spectrum as information and display it. The invention is characterized in that the light bundle passes a first optical element, and then wavelength partial ranges of a spectrally split light bundle impinge on respective partial regions of a diffraction grating, the diffraction grating having the same grating constant across all partial regions and a changing profile shape, the profile shapes generating different blaze wavelengths that lie in the respective wavelength partial ranges.
摘要翻译：本发明涉及一种具有衍射光栅的高灵敏度光谱分析单元，其中具有波长范围的平行光束照射在衍射光栅上，衍射光栅通过在第一方向上的衍射将不同波长分裂成光谱，并且光谱的波长部分范围分光束可以通过相机光学器件聚焦在检测器行上，并且评估电子器件连接到检测器行并且获取所生成的光谱作为信息并显示它。本发明的特征在于，光束通过第一光学元件，然后将光谱分裂的光束的部分范围波动在衍射光栅的各个部分区域上，衍射光栅在所有部分区域具有相同的光栅常数，并且改变轮廓形状，产生位于相应波长部分范围内的不同火焰波长的轮廓形状。

2. 发明申请

US20100007878A1 HIGHLY SENSITIVE SPECTROSCOPIC UNIT 有权
标题翻译：高灵敏度光谱仪
公开(公告)号：US20100007878A1
公开(公告)日：2010-01-14
申请号：US12444244
申请日：2007-10-02
申请人： Ralf Wolleschensky , Hans-Juergen Dobschal , Reinhard Steiner
发明人： Ralf Wolleschensky , Hans-Juergen Dobschal , Reinhard Steiner
IPC分类号： G01J3/18 , G01J3/36 , G01J3/28
CPC分类号： G01J3/1804 , G01J3/02 , G01J3/021 , G01J3/0291 , G01J3/14 , G01J3/26 , G01J3/2803
摘要： The invention is directed to a highly sensitive spectrum analysis unit with a diffraction grating, wherein a parallel light bundle having a wavelength range impinges on a diffraction grating which splits the different wavelengths into spectra by diffraction in first directions, and wavelength partial ranges of the spectrally split light bundle can be focused on a detector row by means of camera optics, and evaluation electronics are connected to the detector row and acquire the generated spectrum as information and display it. The invention is characterized in that the light bundle passes a first optical element, and then wavelength partial ranges of a spectrally split light bundle impinge on respective partial regions of a diffraction grating, the diffraction grating having the same grating constant across all partial regions and a changing profile shape, the profile shapes generating different blaze wavelengths that lie in the respective wavelength partial ranges.
摘要翻译：本发明涉及一种具有衍射光栅的高灵敏度光谱分析单元，其中具有波长范围的平行光束照射在衍射光栅上，衍射光栅通过在第一方向上的衍射将不同波长分裂成光谱，并且光谱的波长部分范围分光束可以通过相机光学器件聚焦在检测器行上，并且评估电子器件连接到检测器行并且获取所生成的光谱作为信息并显示它。本发明的特征在于，光束通过第一光学元件，然后将光谱分裂的光束的部分范围波动在衍射光栅的各个部分区域上，衍射光栅在所有部分区域具有相同的光栅常数，并且改变轮廓形状，产生位于相应波长部分范围内的不同火焰波长的轮廓形状。

3. 发明授权

US07852474B2 Spectral analysis unit with a diffraction grating 有权
$Spectral analysis unit with a diffraction grating$
标题翻译：具有衍射光栅的光谱分析单元
公开(公告)号：US07852474B2
公开(公告)日：2010-12-14
申请号：US11785153
申请日：2007-04-16
申请人： Hans-Juergen Dobschal , Ralf Wolleschensky , Wolfgang Bathe , Joerg Steinert
发明人： Hans-Juergen Dobschal , Ralf Wolleschensky , Wolfgang Bathe , Joerg Steinert
IPC分类号： G01J3/28
CPC分类号： G02B21/0064 , G01J3/02 , G01J3/0208 , G01J3/021 , G01J3/0259 , G01J3/1804 , G02B21/16
摘要： A spectral analytical unit for acting on a parallel light bundle having different wavelengths. The spectral analytical unit includes a diffraction grating on which the light bundle falls, the diffraction grating splitting the different wavelengths through diffraction in first spectral directions defining a light bundle diffraction order 1 without recycle, and the diffraction grating bending the light bundle in second directions defining a light bundle diffraction order 0 without recycle, a detector line made up of a plurality of elements, optics for focusing the split light bundle diffraction order 1 without recycle on the detector line, evaluation electronics connected to the detector line for obtaining data related to a created spectrum, and a deflecting device wherein the diffraction order 0 light bundle without recycle meets on the deflecting device which is so directed and positioned that this light bundle falls on the diffraction grating thereby creating a reflected diffraction order 1 light bundle with first recycle and a reflected diffraction order 0 light bundle with first recycle whereby the diffraction order 1 without recycle and the reflected diffraction order 1 light bundle with first recycle each of a part wavelength range are impressed through the optics on a single element of the detector line.
摘要翻译：用于作用于具有不同波长的平行光束的光谱分析单元。光谱分析单元包括光束落在其上的衍射光栅，衍射光栅通过在限定光束衍射级数1而不进行再循环的第一光谱方向上通过衍射分离不同波长，并且衍射光栅在限定第二方向的第二方向上折射光束没有再循环的光束衍射级0，由多个元件组成的检测器线，用于将分束光束衍射级1聚焦在检测器线路上而不再循环的光学器件，连接到检测器线的评估电子设备，用于获得与以及偏转装置，其中没有再循环的衍射级0光束在偏转装置上相遇，所述偏转装置被定向和定位成使得该光束落在衍射光栅上，从而产生具有第一再循环的反射衍射级1光束和反射衍射级0光束与fi 第一次循环，其中没有再循环的衍射级1和具有部分波长范围的第一次再循环的反射衍射级1光束通过检测器线的单个元件上的光学器件施加。

4. 发明申请

US20070242268A1 Spectral analytical unit with a diffraction grating 有权
$Spectral analytical unit with a diffraction grating$
标题翻译：具有衍射光栅的光谱分析单元
公开(公告)号：US20070242268A1
公开(公告)日：2007-10-18
申请号：US11785153
申请日：2007-04-16
申请人： Hans-Juergen Dobschal , Ralf Wolleschensky , Wolfgang Bathe , Joerg Steinert
发明人： Hans-Juergen Dobschal , Ralf Wolleschensky , Wolfgang Bathe , Joerg Steinert
IPC分类号： G01J3/28
CPC分类号： G02B21/0064 , G01J3/02 , G01J3/0208 , G01J3/021 , G01J3/0259 , G01J3/1804 , G02B21/16
摘要： A spectral analytical unit for acting on a parallel light bundle having different wavelengths. The spectral analytical unit includes a diffraction grating on which the light bundle falls, the diffraction grating splitting the different wavelengths through diffraction in first spectral directions defining a light bundle diffraction order 1 without recycle, and the diffraction grating bending the light bundle in second directions defining a light bundle diffraction order 0 without recycle, a detector line made up of a plurality of elements, optics for focusing the split light bundle diffraction order 1 without recycle on the detector line, evaluation electronics connected to the detector line for obtaining data related to a created spectrum, and a deflecting device wherein the diffraction order 0 light bundle without recycle meets on the deflecting device which is so directed and positioned that this light bundle falls on the diffraction grating thereby creating a reflected diffraction order 1 light bundle with first recycle and a reflected diffraction order 0 light bundle with first recycle whereby the diffraction order 1 without recycle and the reflected diffraction order 1 light bundle with first recycle each of a part wavelength range are impressed through the optics on a single element of the detector line.
摘要翻译：用于作用于具有不同波长的平行光束的光谱分析单元。光谱分析单元包括光束落在其上的衍射光栅，衍射光栅通过在限定光束衍射级数1而不进行再循环的第一光谱方向上通过衍射分离不同波长，并且衍射光栅在限定第二方向的第二方向上折射光束没有再循环的光束衍射级0，由多个元件组成的检测器线，用于将分束光束衍射级1聚焦在检测器线路上而不再循环的光学器件，连接到检测器线的评估电子设备，用于获得与以及偏转装置，其中没有再循环的衍射级0光束在偏转装置上相遇，所述偏转装置被定向和定位成使得该光束落在衍射光栅上，从而产生具有第一再循环的反射衍射级1光束和反射衍射级0光束与fi 第一次循环，其中没有再循环的衍射级1和具有部分波长范围的第一次再循环的反射衍射级1光束通过检测器线的单个元件上的光学器件施加。

5. 发明授权

US09588328B2 Wide-field microscope and method for wide-field microscopy 有权
标题翻译：宽视场显微镜和广视野显微镜的方法
公开(公告)号：US09588328B2
公开(公告)日：2017-03-07
申请号：US14112519
申请日：2012-03-05
申请人： Thomas Kalkbrenner , Ralf Wolleschensky , Ingo Kleppe
发明人： Thomas Kalkbrenner , Ralf Wolleschensky , Ingo Kleppe
IPC分类号： G02B21/36 , G01N21/64 , G02B21/16 , G02B27/58 , H04N5/372
CPC分类号： G02B21/365 , G01N21/6408 , G01N21/6458 , G02B21/16 , G02B21/361 , G02B21/367 , G02B27/58 , H04N5/372
摘要： A method for high-resolution PAL microscopy, wherein a sample field is imaged on a detector surface of a detector, the sample field is imaged into an image field which is smaller than the detector surface, and the image field on the detector surface is shifted, so that the same sample field is imaged in different positions located adjacent to one another on the image field in order to determine information about changes in the sample field.
摘要翻译：一种用于高分辨率PAL显微镜的方法，其中将样品场成像在检测器的检测器表面上，将样本场成像为小于检测器表面的图像场，并且检测器表面上的图像场偏移，使得相同的采样场成像在图像场上彼此相邻的不同位置，以便确定关于样本场中的变化的信息。

6. 发明申请

US20140133011A1 CONFOCAL INCIDENT-LIGHT SCANNING MICROSCOPE 有权
标题翻译：共焦事件光扫描显微镜
公开(公告)号：US20140133011A1
公开(公告)日：2014-05-15
申请号：US14130966
申请日：2012-07-05
申请人： Daniel Schwedt , Ralf Wolleschensky
发明人： Daniel Schwedt , Ralf Wolleschensky
IPC分类号： G02B21/00 , G02F1/29 , G02F1/35
CPC分类号： G02B21/0076 , G02B21/0032 , G02F1/29 , G02F1/353
摘要： A microscope which makes possible a spectrally-flexible excitation and detection of fluorescence in an economical manner. For this purpose, means for frequency conversion are arranged in the common beam path and a filter for excitation light is arranged in addition to the main beam splitter in the detection beam path. The frequency conversion achieves a spectral delimitation between illumination light, which is emitted by the light source, and excitation light which brings about fluorescence excitation in the specimen. Because the frequency conversion takes place in the common beam path after the main beam splitter, it is possible for both a spatial separation of illumination light, and excitation light and fluorescent light (detection light) emitted by the specimen, to be carried out in an economical manner at the main beamsplitter according to spectral bands because of the spectral difference between illumination light and excitation light.
摘要翻译：一种显微镜，可以以经济的方式进行光谱灵活的荧光激发和检测。为此，在公共光束路径中布置用于频率转换的装置，并且除了检测光束路径中的主分束器之外还布置有用于激发光的滤光器。频率转换实现了由光源发射的照明光与在样本中产生荧光激发的激发光之间的光谱定界。由于在主分束器之后的共同光束路径中发生频率转换，所以可以在照射光和激发光的空间分离以及由样本发射的荧光（检测光）两者中进行由于照明光和激发光之间的光谱差异，根据光谱带在主分束器处经济。

7. 发明授权

US08699130B2 Microscope, in particular laser scanning microscope 有权
标题翻译：显微镜，特别是激光扫描显微镜
公开(公告)号：US08699130B2
公开(公告)日：2014-04-15
申请号：US13255627
申请日：2010-03-06
申请人： Ralf Wolleschensky
发明人： Ralf Wolleschensky
IPC分类号： G02B21/36 , G02B21/00
CPC分类号： G02B21/0076 , G01J3/02 , G01J3/0237 , G01J3/26 , G01J3/36 , G01J3/4406 , G01J2003/1221 , G01N21/645 , G01N2021/6471 , G02B21/0064 , G02B27/1006
摘要： Microscope, particularly laser scanning microscope, for optical detection of light radiation excited in a specimen, having a detection beam path for detecting spectral components of the light radiation in a plurality of detection channels, wherein the light radiation arrives at a variable longpass filter or shortpass filter from which reflected and/or transmitted components are reflected back with a parallel offset, and the latter arrive at a detector after at least one back-reflection of this kind.
摘要翻译：显微镜，特别是激光扫描显微镜，用于在样本中激发的光辐射的光学检测，具有用于检测多个检测通道中的光辐射的光谱分量的检测光束路径，其中光辐射到达可变长通滤波器或短路反射和/或透射的分量由平行偏移反射回来的滤光器，后者在这种至少一次背反射之后到达检测器。

8. 发明申请

US20110176206A1 Microscope Having an Adjustment Device for the Focus Range 有权
标题翻译：具有调焦装置的显微镜用于对焦范围
公开(公告)号：US20110176206A1
公开(公告)日：2011-07-21
申请号：US13121886
申请日：2009-09-22
申请人： Ralf Wolleschensky , Michael Kempe
发明人： Ralf Wolleschensky , Michael Kempe
IPC分类号： G02B21/00
CPC分类号： G02B21/006 , G02B21/0068 , G02B21/241 , G02B2207/114
摘要： A microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of the light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted in the direction of the optical axis, with at least one second mirror for transmitting light from the first objective lens in the direction of the second objective lens and from the second objective lens to the detector being disposed in the optical path, which second mirror is a fully reflective mirror, or a microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted in the direction of the optical axis, with a polarizing beam splitter for splitting the object light into two components that are oriented perpendicular to each other being disposed between the first and the second objective lens for light transmission.
摘要翻译：一种具有用于调节焦距范围的装置的显微镜，包括用于在检测器的方向上传送被照射物体的物体的第一物镜，其中第二物镜沿检测器上游的光的方向设置，第二物镜之后是可以在光轴方向上调节的第一反射镜，具有至少一个第二反射镜，用于在第二物镜的方向上从第一物镜透射光并从第二物镜传播光所述检测器设置在所述光路中，所述第二反射镜是完全反射镜，或具有用于调节所述焦距的装置的显微镜，所述显微镜包括用于沿检测器的方向透射被照射物体的物体的第一物镜，其中第二物镜沿着检测器上游的光的方向设置，该第二物镜后面是第一镜像 r可以在光轴的方向上调整，其中偏振分束器用于将物体光分成两个彼此垂直取向的分量，这两个分量被布置在第一和第二物镜之间用于透光。

9. 发明申请

US20110036996A1 APPARATUS AND METHOD FOR HIGH SPATIAL RESOLUTION IMAGING OF A STRUCTURE OF A SAMPLE 有权
标题翻译：用于样品结构的高空间分辨率成像的装置和方法
公开(公告)号：US20110036996A1
公开(公告)日：2011-02-17
申请号：US12867291
申请日：2009-02-03
申请人： Ralf Wolleschensky , Helmut Lippert , Christopher Power , Benno Radt
发明人： Ralf Wolleschensky , Helmut Lippert , Christopher Power , Benno Radt
IPC分类号： G01J1/58
CPC分类号： G02B21/002 , G01N21/6458 , G02B21/10 , G02B27/58
摘要： Apparatus and method for high spatial resolution imaging of a sample's structure, including a diffraction-limited resolution volume with a plurality of dye molecules which can be switched between different states and have a distribution density which is greater than the inverse of the diffraction-limited resolution volume, where at least one state is fluorescing, the fluorescence being collected by an objective lens and imaged on a spatially resolving detector by an optical system. At least one light source provided for emitting a switching radiation and for emitting an excitation radiation. At least one of the light sources is arranged to radiate through the sample, and a switching and/or fluorescence excitation of the dye molecules is carried out. The switching is a photoactivation or a photodeactivation of the dye molecules. A focusing arrangement is provided for switching and/or for excitation to generate a line-like illumination region extending in a direction of illumination.
摘要翻译：用于样品结构的高空间分辨率成像的装置和方法，包括具有多个染料分子的衍射限制分辨率体积，其可以在不同状态之间切换并且具有大于衍射受限分辨率的反比的分布密度体积，其中至少一个状态是荧光的，荧光由物镜收集并通过光学系统在空间分辨检测器上成像。提供用于发射切换辐射并用于发射激发辐射的至少一个光源。至少一个光源布置成通过样品辐射，并且进行染料分子的切换和/或荧光激发。切换是染料分子的光活化或光激活。提供了用于切换和/或激发的聚焦装置，以产生在照明方向上延伸的线状照明区域。

10. 发明授权

US07872799B2 Device for controlling light radiation 有权
标题翻译：用于控制光辐射的装置
公开(公告)号：US07872799B2
公开(公告)日：2011-01-18
申请号：US12318287
申请日：2008-12-23
申请人： Ralf Wolleschensky
发明人： Ralf Wolleschensky
IPC分类号： G02B21/06
CPC分类号： G02B27/283 , G01J3/0224 , G01J3/1256 , G01J3/4406 , G01J3/447 , G01N21/6445 , G01N21/6458 , G02B21/002 , G02B21/0068
摘要： Device for controlling light radiation, which is excited in a specimen and/or which is backscattered and/or reflected and which contains one or more wavelengths, at a plurality of light outlets, wherein a separation of the light radiation into differently polarized components is carried out; and the components of the excitation radiation and/or detection radiation are affected in their polarization by means of a preferably birefringent, preferably acousto-optic or electro-optic medium, which changes the ordinary and extraordinary refractive index.
摘要翻译：用于控制在多个光出口处在样本中激发并且/或背散射和/或反射并且包含一个或多个波长的光辐射的装置，其中将光辐射分离成不同偏振的分量出来并且激发辐射和/或检测辐射的分量通过改变普通和非凡折射率的优选双折射，优选声光或电光介质在它们的极化中受到影响。

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