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    • 9. 发明申请
    • Methods and Compositions for Increasing the Nitrogen Storage Capacity of a Plant
    • 提高植物氮储存能力的方法和组成
    • US20110004960A1
    • 2011-01-06
    • US12828428
    • 2010-07-01
    • Kanwarpal S. DhuggaLaura M. AppenzellerRajeev GuptaHari Kishan Rao Abbaraju
    • Kanwarpal S. DhuggaLaura M. AppenzellerRajeev GuptaHari Kishan Rao Abbaraju
    • A01H1/00C12N15/82A01H5/00G01N33/68A01H5/10
    • C07K14/415C12N15/8242C12N15/8251C12N15/8261Y02A40/146
    • The present invention provides methods and compositions for making and using transgenic plants that exhibit increased nitrogen storage capacity compared to wild-type plants. Methods of the invention comprise inducing overexpression of monocot-derived vegetative storage proteins (VSPs) in plants, particularly in monocots. In some embodiments, at least one nucleotide construct comprising a nucleotide sequence encoding the ZmLox6 protein or a biologically active fragment or variant thereof is introduced into a plant. Depending upon the objective, the nucleotide construct may optionally comprise an operably linked coding sequence for a vacuolar sorting signal or plastid transit peptide in order to direct storage of the ZmLox6 protein or biologically active fragment or variant thereof into the vacuolar compartment or plastid compartment, respectively, of the cells in which the VSP is expressed. The invention further provides methods for producing plants with increased nitrogen content and/or increased nutritional value, which is desirable in commercial crops, including those used for forage, silage, and grain production.
    • 与野生型植物相比,本发明提供了制备和使用表现出增加的氮储存能力的转基因植物的方法和组合物。 本发明的方法包括在植物中特别是在单子叶植物中诱导单子叶来源的营养贮藏蛋白(VSP)的过度表达。 在一些实施方案中,将包含编码ZmLox6蛋白或其生物活性片段或变体的核苷酸序列的至少一个核苷酸构建体引入植物中。 根据目标,核苷酸构建体可以任选地包含用于液泡分选信号或质体转运肽的可操作地连接的编码序列,以便将ZmLox6蛋白或其生物活性片段或变体分别引导到液泡区或质体隔室中 ,其中表达VSP的细胞。 本发明还提供了用于生产具有增加的氮含量和/或增加的营养价值的植物的方法,其在商业作物中是需要的,包括用于饲料,青贮饲料和谷粒生产的农作物。