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1. 发明授权

US07151633B2 Scanning microscope 有权
标题翻译：扫描显微镜
公开(公告)号：US07151633B2
公开(公告)日：2006-12-19
申请号：US10903139
申请日：2004-07-30
申请人： Rafael Storz , Werner Knebel
发明人： Rafael Storz , Werner Knebel
IPC分类号： G02B21/06
CPC分类号： G02B21/0076 , G02B21/002 , G02B21/0032 , G02B21/008
摘要： A detector, arranged in the detection beam path of a scanning microscope, for the detection of detected light proceeding from a sample can be used to detect other than the detected light for example of external optical experiments. The scanning microscope comprises an incoupling apparatus with which light other than the detected light can be coupled into the detection beam path and conveyed to the detector.
摘要翻译：布置在扫描显微镜的检测光束路径中的用于检测从样品进行的检测光的检测器可以用于检测例如外部光学实验的检测到的光。扫描显微镜包括一个去耦装置，除了检测到的光之外，其中的光可以被耦合到检测光束路径中并传送到检测器。

2. 发明授权

US07477380B2 Scanning microscope comprising a confocal slit scanner for imaging an object 有权
标题翻译：扫描显微镜，包括用于成像物体的共焦狭缝扫描器
公开(公告)号：US07477380B2
公开(公告)日：2009-01-13
申请号：US10537335
申请日：2003-09-23
申请人： Werner Knebel , Holger Birk , Rafael Storz
发明人： Werner Knebel , Holger Birk , Rafael Storz
IPC分类号： G01J3/28
CPC分类号： G02B21/0032 , G02B21/0076 , G02B21/0084
摘要： A scanning microscope for imaging an object includes a light source and a spectrally selective detection device. An illumination beam path extends from the light source to the object. A detection beam path extends from the object to the detection device. A spectrally selective element useable to select light from the light source is provided. The spectrally selective element is useable to mask out of the detection beam path the selected light from the light source reflected or scattered on the object. An illumination slit diaphragm is disposed in the illumination beam path and configured to generate a linear illumination pattern in a region of the object. A detection slit diaphragm is disposed in the detection beam path and configured to detect the light coming from the linear illumination region from a focal plane so as to provide a confocal slit scanner.
摘要翻译：用于对物体成像的扫描显微镜包括光源和光谱选择性检测装置。照明光束路径从光源延伸到物体。检测光束路径从物体延伸到检测装置。提供了可用于选择来自光源的光的光谱选择性元件。光谱选择元件可用于从反射或散射在物体上的光源的光源遮掩出检测光束路径。照明狭缝光阑设置在照明光束路径中并且被配置为在物体的区域中产生线性照明图案。检测狭缝光阑设置在检测光束路径中，并被配置为从焦平面检测来自线性照明区域的光，以提供共焦狭缝扫描器。

3. 发明授权

US07477449B2 Scanning microscope 有权
标题翻译：扫描显微镜
公开(公告)号：US07477449B2
公开(公告)日：2009-01-13
申请号：US10713887
申请日：2003-11-14
申请人： Werner Knebel , Rafael Storz
发明人： Werner Knebel , Rafael Storz
IPC分类号： G02B21/06
CPC分类号： G02B21/002
摘要： A scanning microscope, having a detector, arranged in a detection beam path, for receiving detection light proceeding from a sample, has between the sample and the detector an optical shutter means with which the detection beam path can be blocked. A control means for controlling the shutter means is provided. The detection light beam path can be blocked automatically outside the scanning operation and in the event of an excessive detection light power level.
摘要翻译：具有检测器的扫描显微镜，其布置在检测光束路径中，用于接收从样本进行的检测光，在样品和检测器之间具有可以阻挡检测光束路径的光学快门装置。提供一种用于控制快门装置的控制装置。检测光束路径可以自动地在扫描操作之外和在检测光功率级别过大的情况下被阻挡。

4. 发明授权

US07041951B2 Method and apparatus for investigating layers of tissues in living animals using a microscope 有权
标题翻译：使用显微镜研究活体组织层的方法和装置
公开(公告)号：US07041951B2
公开(公告)日：2006-05-09
申请号：US10700929
申请日：2003-11-04
申请人： Martin Hoppe , Werner Knebel , Kyra Moellmann , Rafael Storz
发明人： Martin Hoppe , Werner Knebel , Kyra Moellmann , Rafael Storz
IPC分类号： G02B27/64 , A61B6/00
CPC分类号： G02B21/241
摘要： The invention concerns a method and an apparatus for investigating layers (1) of tissues in living animals using a microscope (2). The microscope (2) is focused onto a layer (1), and images of the layer (1) are acquired or optical measurements are performed on it. Positional changes of the layer (1) are brought about by movements of the animal or of its organs. The positional changes are sensed, and corresponding signals are generated. The signals are stored, together with the corresponding images or measurement results, for later evaluation; or they are processed in such a way that the positional changes are compensated for in order to investigate the layer (1). As a result, the layer (1) can be qualitatively or quantitatively investigated microscopically, irrespective of the movement of the animal or its organs.
摘要翻译：本发明涉及一种使用显微镜（2）研究活体组织的层（1）的方法和装置。显微镜（2）被聚焦到层（1）上，并且获取层（1）的图像或对其进行光学测量。层（1）的位置变化是由动物或其器官的运动引起的。检测到位置变化，并产生相应的信号。将信号与相应的图像或测量结果一起存储以供稍后评估; 或者它们以这样的方式进行处理，以便补偿位置变化以便研究层（1）。结果，无论动物或其器官的移动如何，层（1）可以在显微镜下进行定性或定量研究。

5. 发明申请

US20050024721A1 Scanning microscope 有权
标题翻译：扫描显微镜
公开(公告)号：US20050024721A1
公开(公告)日：2005-02-03
申请号：US10903139
申请日：2004-07-30
申请人： Rafael Storz , Werner Knebel
发明人： Rafael Storz , Werner Knebel
IPC分类号： G02B21/06 , G02B21/00
CPC分类号： G02B21/0076 , G02B21/002 , G02B21/0032 , G02B21/008
摘要： A detector, arranged in the detection beam path of a scanning microscope, for the detection of detected light proceeding from a sample can be used to detect other than the detected light for example of external optical experiments. The scanning microscope comprises an incoupling apparatus with which light other than the detected light can be coupled into the detection beam path and conveyed to the detector.
摘要翻译：布置在扫描显微镜的检测光束路径中的用于检测从样品进行的检测光的检测器可以用于检测除了例如外部光学实验的检测到的光。扫描显微镜包括一个去耦装置，除了检测到的光之外，其中的光可以被耦合到检测光束路径中并传送到检测器。

6. 发明申请

US20060152787A1 Scanning microscope comprising a confocal slit scanner for reproducing an object 有权
标题翻译：扫描显微镜，包括用于再现物体的共聚焦狭缝扫描器
公开(公告)号：US20060152787A1
公开(公告)日：2006-07-13
申请号：US10537335
申请日：2003-09-23
申请人： Werner Knebel , Holger Birk , Rafael Storz
发明人： Werner Knebel , Holger Birk , Rafael Storz
IPC分类号： G02B26/08
CPC分类号： G02B21/0032 , G02B21/0076 , G02B21/0084
摘要： A scanning microscope for imaging an object includes a light source and a spectrally selective detection device. An illumination beam path extends from the light source to the object. A detection beam path extends from the object to the detection device. A spectrally selective element useable to select light from the light source is provided. The spectrally selective element is useable to mask out of the detection beam path the selected light from the light source reflected or scattered on the object. An illumination slit diaphragm is disposed in the illumination beam path and configured to generate a linear illumination pattern in a region of the object. A detection slit diaphragm is disposed in the detection beam path and configured to detect the light coming from the linear illumination region from a focal plane so as to provide a confocal slit scanner.
摘要翻译：用于对物体成像的扫描显微镜包括光源和光谱选择性检测装置。照明光束路径从光源延伸到物体。检测光束路径从物体延伸到检测装置。提供了可用于选择来自光源的光的光谱选择性元件。光谱选择元件可用于从反射或散射在物体上的光源的光源遮掩出检测光束路径。照明狭缝光阑设置在照明光束路径中并且被配置为在物体的区域中产生线性照明图案。检测狭缝光阑设置在检测光束路径中，并被配置为从焦平面检测来自线性照明区域的光，以提供共焦狭缝扫描器。

7. 发明授权

US07087891B2 Scanning microscope having an acoustooptical component 有权
标题翻译：具有声光分量的扫描显微镜
公开(公告)号：US07087891B2
公开(公告)日：2006-08-08
申请号：US10762602
申请日：2004-01-22
申请人： Werner Knebel , Rafael Storz , Kyra Moellmann
发明人： Werner Knebel , Rafael Storz , Kyra Moellmann
IPC分类号： H01J3/14 , H01J5/16 , H01J40/14 , G02F1/33
CPC分类号： G02B21/008 , G02B21/0032 , G02B21/0052 , G02B21/0068 , G02F1/113 , G02F1/13 , G02F2203/07
摘要： A scanning microscope having an acoustooptical component that splits out illuminating light for illumination of a sample from the output light of at least one light source, and conveys detected light proceeding from the sample to a detector, comprises, in the beam path of the output light from which the illuminating light is split out, at least one monitoring detector which is the measuring element of a control circuit. The scanning microscope is characterized in that fluctuations over time in the illuminating light power level are largely eliminated.
摘要翻译：一种扫描显微镜，其具有从至少一个光源的输出光分离用于照射样品的照明光并将从样品进行的检测光传送到检测器的声光分量，包括在输出光的光束路径中照明光从其分出，至少一个监视检测器，其是控制电路的测量元件。扫描显微镜的特征在于，在很大程度上消除了照明光功率电平随时间的波动。

8. 发明授权

US07259366B2 Apparatus for the detection of photons of a light beam having a three-dimensional array of detectors 有权
标题翻译：用于检测具有三维检测器阵列的光束的光子的装置
公开(公告)号：US07259366B2
公开(公告)日：2007-08-21
申请号：US10568002
申请日：2004-04-02
申请人： Holger Birk , Rafael Storz , Volker Seyfried
发明人： Holger Birk , Rafael Storz , Volker Seyfried
IPC分类号： H01L31/00
CPC分类号： G02B21/26
摘要： An apparatus for detecting photons of a light beam emanating from a spatially limited source includes a detection device having a plurality of detectors forming a three-dimensional array with semitranslucent EMCCDs disposed one behind another. A light splitting device is disposed in a path of rays of the light beam for splitting the light beam so as to distribute the photons over the detectors for detection.
摘要翻译：用于检测从空间有限的源发出的光束的光子的装置包括具有多个检测器的检测装置，所述检测器形成具有一个彼此设置的半透明EMCCD的三维阵列。光分离装置设置在光束的光线路径中，用于分离光束，以将光子分布在检测器上用于检测。

9. 发明申请

US20050122580A1 Arrangement for examining microscopic preparations with a scanning microscope, and illumination device for a scanning microscope 有权
公开(公告)号：US20050122580A1
公开(公告)日：2005-06-09
申请号：US11034888
申请日：2005-01-14
申请人： Holger Birk , Rafael Storz
发明人： Holger Birk , Rafael Storz
IPC分类号： G02B5/00 , G02B6/00 , G02B6/02 , G02B6/122 , G02B6/255 , G02B21/00 , G02B21/06 , G02B27/00 , H01S3/00 , H01S3/16 , A61B18/18
CPC分类号： G02B6/02376 , B82Y20/00 , G02B6/02366 , G02B6/02371 , G02B6/1225 , G02B6/2552 , G02B21/002 , G02B21/0024 , G02B21/0032 , G02B21/0056 , G02B21/0064 , G02B21/0076 , G02B21/008 , G02B21/06 , H01S3/005 , H01S3/1625 , H01S3/1636
摘要： The arrangement for examining microscope preparations with a scanning microscope comprises a laser (1) and an optical means (12) which images the light generated by the laser (1) onto a specimen (13) that is to be examined. Provided between the laser (1) and the optical means (12) is an optical component (3, 20) that spectrally spreads, with a single pass, the light generated by the laser (1). The optical component (3, 20) is made of photonic band-gap material. It is particularly advantageous if the photonic band-gap material is configured as a light-guiding fiber (20).

10. 发明授权

US06864989B2 Method for illuminating an object with light from a laser light source 有权
标题翻译：用于用来自激光光源的光照射物体的方法
公开(公告)号：US06864989B2
公开(公告)日：2005-03-08
申请号：US09939726
申请日：2001-08-28
申请人： Rafael Storz , Johann Engelhardt , Holger Birk
发明人： Rafael Storz , Johann Engelhardt , Holger Birk
IPC分类号： G02B26/06 , G02B21/00 , G02B21/06 , G02F1/01 , H01S3/00 , G01B11/24
CPC分类号： G02B21/0024 , G02B21/0056
摘要： A method for illuminating an object with light (2) from a laser light source (3), preferably in a confocal scanning microscope (1). With the method according to the invention, it is possible to reduce the coherence length of the laser light, so that disruptive interference phenomena can be substantially eliminated. Should interference phenomena nevertheless be formed, these are to be influenced in such a way that they have no effect on the detection. The method according to the invention is characterized in that the phase angle of the light field is varied by a modulator in such a way that interference phenomena do not occur in the optical beam path, or occur only to an undetectable extent, within a predeterminable time interval.
摘要翻译：优选在共焦扫描显微镜（1）中从激光光源（3）用光（2）照射物体的方法。利用根据本发明的方法，可以减少激光的相干长度，从而可以基本上消除破坏性干扰现象。应该如何形成干扰现象，这些影响将不会对检测产生影响。根据本发明的方法的特征在于，光场的相位角由调制器改变，使得在预定时间内在光束路径中不发生干涉现象或仅发生不可检测的程度间隔。

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