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    • 7. 发明申请
    • Computer-assisted analysis
    • 计算机辅助分析
    • US20060154236A1
    • 2006-07-13
    • US11271215
    • 2005-11-11
    • Steven AltschulerLani WuMichael Slack
    • Steven AltschulerLani WuMichael Slack
    • C12Q1/00G06F19/00
    • G01N33/5026
    • The present invention provides methods and systems for automated morphological analysis of cells also known as phenotypic screening. The inventive methods are particularly useful in the rapid analysis of cells required in a biological screen or in the screening for agents with a particular mechanism of action. Agents which cause a particular phenotype in the cells can be identified using the inventive quantitative morphometric analysis of cells. The data gathered using the inventive method can also be quantified and analyzed later for various trends and classifications (e.g., Kolmogorov-Smirnov statistics, titration-invariant similarity scores). Characteristics of cells which can be determined using this method include number of nuclei, size of cell, size of nuclei, number of the centrosomes, shape of cells, size of centrosomes, perimeter of nucleus, shape of nucleus, staining for a particular protein, staining for an organelle, pattern of staining, and degree of staining.
    • 本发明提供了也称为表型筛选的细胞的自动形态学分析的方法和系统。 本发明的方法在生物屏幕中所需的细胞的快速分析或具有特定作用机制的试剂的筛选中特别有用。 可以使用本发明的细胞定量形态学分析来鉴定在细胞中引起特定表型的药剂。 使用本发明方法收集的数据也可以针对各种趋势和分类进行量化和分析(例如,Kolmogorov-Smirnov统计,滴定 - 不变相似性评分)。 可以使用该方法确定的细胞的特征包括细胞核的数量,细胞的大小,细胞核的大小,中心体的数量,细胞的形状,中心体的大小,细胞核的周长,细胞核的形状,特定蛋白质的染色, 染色细胞器,染色图案和染色程度。