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1. 发明申请

US20020028457A1 Single target counting assays using semiconductor nanocrystals 审中-公开
标题翻译：使用半导体纳米晶体的单目标计数测定
公开(公告)号：US20020028457A1
公开(公告)日：2002-03-07
申请号：US09882193
申请日：2001-06-13
申请人： Quantum Dot Corporation
发明人： Stephen Alexander Empedocles , Andrew R. Watson , Vince Phillips , Edith Wong
IPC分类号： C12Q001/68
CPC分类号： B82Y15/00 , G01N33/588
摘要： The present invention provides assays that allow for the detection of a single copy of a target of interest. The target species is either directly or indirectly labeled with a semiconductor nanocrytal, nullquantum dot.null The bright and tunable fluorescence of the quantum dot is readily detected using methods described herein. Also provided are assays that are based on the colocalization of two or more differently colored quantum dots on a single target species, which provides superbly sensitive assays in which the decrease in assay sensitivity caused by non-specific binding of assay mixture components to the assay substrate is minimized. The assays are of use to detect target species including, but are not limited to, nucleic acids, polypeptides, small organic bioactive agents (e.g., drugs, agents of war, herbicides, pesticides, etc.) and organisms.
摘要翻译：本发明提供允许检测感兴趣的靶标的单拷贝的测定。目标物种直接或间接用半导体纳米碳“”量子点“标记。使用本文所述的方法容易地检测量子点的明亮和可调谐的荧光。还提供了基于在单个靶物种上两种或更多种不同颜色的量子点共定位的测定法，其提供了非常敏感的测定，其中由测定混合物组分非特异性结合到测定底物引起的测定灵敏度降低被最小化。该测定法用于检测靶物种，包括但不限于核酸，多肽，小有机生物活性剂（例如药物，战争剂，除草剂，农药等）和生物体。

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