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1. 发明授权

US5679533A Biotin-binding containment systems 失效
公开(公告)号：US5679533A
公开(公告)日：1997-10-21
申请号：US479390
申请日：1995-06-07
申请人： Przemyslaw Szafranski , Charlene M. Mello , Takeshi Sano , Kenneth A. Marx , Charles R. Cantor , David L. Kaplan , Cassandra L. Smith
发明人： Przemyslaw Szafranski , Charlene M. Mello , Takeshi Sano , Kenneth A. Marx , Charles R. Cantor , David L. Kaplan , Cassandra L. Smith
IPC分类号： C07K14/36 , C07K14/465 , C12N1/21 , C12N15/12 , C12N15/70 , G01N33/53
CPC分类号： C12N15/70 , C07K14/36 , C07K14/465
摘要： The present invention relates to genetic containment systems which express a biotin-binding component that can be used for selectively destroying recombinant cells such as genetically engineered microorganisms. These systems may comprise a streptavidin or an avidin gene whose expression is controlled by a regulatable promoter. The regulatory agent such as a transcriptional effector is expressed from another gene which may also be expressed and its expression controlled by the containment system. Expression of the agent can be designed to respond to physiological changes in the environment. The invention also relates to containment systems and methods for the selective detection or tracking of recombinant cells and to eukaryotic and prokaryotic cells which contain these genetic containment systems.

2. 发明授权

US5681745A Biotin-binding containment systems 失效
标题翻译：生物素结合遏制系统
公开(公告)号：US5681745A
公开(公告)日：1997-10-28
申请号：US432017
申请日：1995-05-01
申请人： Przemyslaw Szafranski , Charlene M. Mello , Takeshi Sano , Kenneth A. Marx , Charles R. Cantor , David L. Kaplan , Cassandra L. Smith
发明人： Przemyslaw Szafranski , Charlene M. Mello , Takeshi Sano , Kenneth A. Marx , Charles R. Cantor , David L. Kaplan , Cassandra L. Smith
IPC分类号： C07K14/36 , C07K14/465 , C12N1/21 , C12N15/12 , C12N15/70 , C12N5/10 , C12N1/13
CPC分类号： C12N15/70 , C07K14/36 , C07K14/465
摘要： The present invention relates to genetic containment systems which express a biotin-binding component that can be used for selectively destroying recombinant cells such as genetically engineered microorganisms. These systems may comprise a streptavidin or an avidin gene whose expression is controlled by a regulatable promoter. The regulatory agent such as a transcriptional effector is expressed from another gene which may also be expressed and its expression controlled by the containment system. Expression of the agent can be designed to respond to physiological changes in the environment. The invention also relates to containment systems and methods for the selective detection or tracking of recombinant cells and to eukaryotic and prokaryotic cells which contain these genetic containment systems.
摘要翻译：本发明涉及表达可用于选择性破坏重组细胞如遗传工程微生物的生物素结合成分的遗传容纳系统。这些系统可以包含链霉亲和素或抗生物素蛋白基因，其表达受可调节启动子控制。诸如转录效应子的调节剂由另外可能被表达的基因表达，其表达由遏制系统控制。试剂的表达可以设计成响应环境中的生理变化。本发明还涉及用于选择性检测或跟踪重组细胞和含有这些遗传包涵体系的真核和原核细胞的遏制系统和方法。

3. 发明授权

US09175422B2 Polymer-micelle complex as an aid to electrospinning 有权
公开(公告)号：US09175422B2
公开(公告)日：2015-11-03
申请号：US11998981
申请日：2007-11-05
申请人： Ramanathan Nagarajan , Christopher P. Drew , Charlene M. Mello
发明人： Ramanathan Nagarajan , Christopher P. Drew , Charlene M. Mello
IPC分类号： B29C69/00 , D01D5/00 , D01F1/10
CPC分类号： D01D5/003 , D01F1/10 , Y10T428/298
摘要： A polymer-micelle complex suitable for use as an aid to preparing fibers, particularly nanofibers, by electrospinning. The polymer-micelle complex may be designed to impart viscosity, surface tension and conductivity properties optimal for electrospinning. By incorporating the complex as a secondary ingredient, one may electrospin sparingly soluble or low molecular weight polymers. Moreover, the polymer-micelle complex can be used as a generic carrier for preparing fibers incorporating other desired materials, such as rigid or globular (hard-to-spin) polymers, enzymes, cells, viral particles and nanoparticles.

4. 发明申请

US20100240121A1 Electrospun nanofibrous membrane assembly for use in capturing chemical and/or biological analytes 审中-公开
标题翻译：用于捕获化学和/或生物分析物的Electrospun纳米纤维膜组件
公开(公告)号：US20100240121A1
公开(公告)日：2010-09-23
申请号：US11388175
申请日：2006-03-24
申请人： Kris J. Senecal , Andre G. Senecal , Philip E. Pivarnik , Charlene M. Mello , Jason W. Soares , Heidi L. Schreuder-Gibson
发明人： Kris J. Senecal , Andre G. Senecal , Philip E. Pivarnik , Charlene M. Mello , Jason W. Soares , Heidi L. Schreuder-Gibson
IPC分类号： C12M1/34
CPC分类号： B01D39/1623 , B01D2239/0216 , B01D2239/025 , D04H1/4291 , D04H1/4334 , D04H1/4358 , D04H1/4382
摘要： A membrane assembly adapted for use in capturing an analyte of interest, the membrane assembly comprising in one embodiment (a) an electrospun nanofibrous membrane, the electrospun nanofibrous membrane comprising a random mat of electrospun nanofibers, at least some of the electrospun nanofibers including one or more types of functional groups; and (b) at least one molecular recognition element immobilized on the random mat via a functional group, the molecular recognition element being adapted to selectively bind the analyte of interest. The membrane assembly may be incorporated into a sensor.
摘要翻译：一种适于用于捕获感兴趣分析物的膜组件，所述膜组件包括在一个实施方案中（a）电纺丝纳米纤维膜，所述电纺丝纳米纤维膜包含电纺丝纳米纤维的无规毡，至少一些所述电纺丝纳米纤维包括一个或多个更多类型的功能组; 和（b）经由官能团固定在无规毡上的至少一种分子识别元件，所述分子识别元件适于选择性地结合感兴趣的分析物。膜组件可以结合到传感器中。

5. 发明申请

US20090081457A1 Polymer-micelle complex as an aid to electrospinning 审中-公开
标题翻译：聚合物胶束复合物作为电纺丝的辅助
公开(公告)号：US20090081457A1
公开(公告)日：2009-03-26
申请号：US11998981
申请日：2007-11-05
申请人： Ramanathan Nagarajan , Christopher P. Drew , Charlene M. Mello
发明人： Ramanathan Nagarajan , Christopher P. Drew , Charlene M. Mello
IPC分类号： B32B5/02 , B29C47/00 , C08F301/00 , C12N11/08
CPC分类号： D01D5/003 , D01F1/10 , Y10T428/298
摘要： A polymer-micelle complex suitable for use as an aid to preparing fibers, particularly nanofibers, by electrospinning. The polymer-micelle complex may be designed to impart viscosity, surface tension and conductivity properties optimal for electrospinning. By incorporating the complex as a secondary ingredient, one may electrospin sparingly soluble or low molecular weight polymers. Moreover, the polymer-micelle complex can be used as a generic carrier for preparing fibers incorporating other desired materials, such as rigid or globular (hard-to-spin) polymers, enzymes, cells, viral particles and nanoparticles.
摘要翻译：一种聚合物胶束复合物，适用于通过静电纺丝制备纤维，特别是纳米纤维。聚合物 - 胶束复合物可以设计成赋予静电纺丝最佳的粘度，表面张力和导电性能。通过掺入复合物作为次要成分，可以静电纺丝微溶或低分子量聚合物。此外，聚合物 - 胶束复合物可以用作制备掺入其它所需材料的纤维的通用载体，例如刚性或球状（难以旋转的）聚合物，酶，细胞，病毒颗粒和纳米颗粒。

6. 发明授权

US07033769B2 Method for discovering one or more peptides adapted for specific binding to a microorganism of interest 失效
标题翻译：用于发现适于特异性结合感兴趣的微生物的一种或多种肽的方法
公开(公告)号：US07033769B2
公开(公告)日：2006-04-25
申请号：US10153524
申请日：2002-05-23
申请人： Charlene M. Mello , Steven Michael Arcidiacono , Jason William Soares
发明人： Charlene M. Mello , Steven Michael Arcidiacono , Jason William Soares
IPC分类号： G01N33/53
CPC分类号： C40B30/04 , C12N15/1034 , G01N33/6845
摘要： A method for discovering one or more peptides adapted for specific binding to a microorganism of interest. The method comprises (i) identifying an antimicrobial peptide having antimicrobial activity against the microorganism of interest, (ii) generating a library of first generation mutants of the antimicrobial peptide, each of the first generation mutants differing from the antimicrobial peptide by a small number of amino acid substitutions, additions or deletions, (iii) screening the library of first generation mutants for those first generation mutants that bind to the microorganism of interest, (iv) determining the peptide sequences of those first generation mutants that bind to the microorganism of interest, and (v) if necessary, repeating steps (ii) through (iv) for one or more successive generations of mutants until one or more consensus peptide sequences emerge.
摘要翻译：一种用于发现一种或多种适于与感兴趣的微生物特异性结合的肽的方法。该方法包括（i）鉴定对目标微生物具有抗微生物活性的抗微生物肽，（ii）产生抗微生物肽的第一代突变体文库，第一代突变体与抗微生物肽不同的少量氨基酸取代，添加或缺失，（iii）筛选与所关注微生物结合的第一代突变体的第一代突变体文库，（iv）测定与感兴趣的微生物结合的那些第一代突变体的肽序列，和（v）如果需要，对于一个或多个连续世代的突变体重复步骤（ii）至（iv），直到出现一个或多个共有肽序列。

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