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1. 发明申请

US20120009575A1 INDUCIBLE NUCLEIC ACID TARGETS FOR DETECTION OF PATHOGENS, METHODS AND COMPOSITIONS THEREOF 审中-公开
标题翻译：用于检测病原体的诱导核酸目标，其方法和组合物
公开(公告)号：US20120009575A1
公开(公告)日：2012-01-12
申请号：US13174645
申请日：2011-06-30
申请人： Priya Balachandran , Craig Cummings , Olga Petrauskene , Hemant Pawar , Robert Tebbs , Manohar Furtado
发明人： Priya Balachandran , Craig Cummings , Olga Petrauskene , Hemant Pawar , Robert Tebbs , Manohar Furtado
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/158 , Y02A50/451
摘要： The present application describes compositions, methods and kits for rapid detection and identification of various microorganisms using inducible RNA. Methods for rapidly detecting microorganisms by detecting expression of inducible RNA of target genes following induction of a target gene using an inducer are described. Some embodiments describe methods and workflows for rapidly detecting microbes such as, but not limited to, Salmonella spp, Listeria spp. and Vibrio spp. Compositions and kits comprise primer nucleic acid sequences having hybridization specificity for priming amplification of genes of microorganisms (or gene fragments) that are responsive to one or more RNA-inducing agents. In some embodiments, kits and compositions further comprise probe nucleic acid sequences having hybridization specificity for genes responsive to RNA-inducing agents or fragments thereof.
摘要翻译：本申请描述了使用可诱导RNA快速检测和鉴定各种微生物的组合物，方法和试剂盒。描述了通过使用诱导剂在靶基因诱导后检测靶基因的诱导型RNA的表达来快速检测微生物的方法。一些实施方案描述了用于快速检测微生物的方法和工作流程，例如但不限于沙门氏菌属，李斯特氏菌属（Listeria spp）。和弧菌属（Vibrio spp。组合物和试剂盒包含对对一种或多种RNA诱导剂有响应的微生物（或基因片段）基因的引物扩增具有杂交特异性的引物核酸序列。在一些实施方案中，试剂盒和组合物还包含对对RNA诱导剂或其片段有响应的基因具有杂交特异性的探针核酸序列。

2. 发明授权

US09175354B2 Detection of Salmonella enterica subspecies enterica serovar Enteritidis in food and environmental samples, methods and compositions therefor 有权
标题翻译：检测食品和环境样品中肠炎沙门氏菌亚型肠炎肠炎沙门氏菌，其方法和组成
公开(公告)号：US09175354B2
公开(公告)日：2015-11-03
申请号：US13197758
申请日：2011-08-03
申请人： Robert Tebbs , Craig Cummings , Arlene Nunez , Priya Balachandran , Payman Fatemi , Olga Petrauskene
发明人： Robert Tebbs , Craig Cummings , Arlene Nunez , Priya Balachandran , Payman Fatemi , Olga Petrauskene
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2561/113 , C12Q2600/156 , Y02A50/451
摘要： Embodiments of the disclosure relate to compositions of isolated nucleic acid sequences, methods, workflows and kits of use thereof for detection of Salmonella enterica, subspecies enterica, serovar Enteritidis (S. Enteritidis) in a sample, particularly in a food (egg, poultry) or environmental sample (including poultry-related environmental samples). Embodiments of the disclosure may also be used to differentially detect S. Enteritidis without cross-reacting with S. Paratyphi C or other closely related Salmonella species. In some embodiments, methods and kits of the disclosure may comprise a TaqMan® assay. Following sample enrichment, methods of detection may be completed in approximately 3 hours.
摘要翻译：本公开的实施方案涉及分离的核酸序列的组合物，方法，工作流程和其用于检测肠炎沙门氏菌，肠亚科，血清型肠炎沙丁杆菌（肠炎沙丁杆菌）的样品中尤其是食品（鸡蛋，家禽）或环境样品（包括家禽相关环境样品）。本公开的实施方案也可用于差异检测肠杆菌，而不与副伤寒杆菌C或其他密切相关的沙门氏菌属物种交叉反应。在一些实施方案中，本公开的方法和试剂盒可以包含TaqMan测定。样品富集后，检测方法可在约3小时内完成。

3. 发明申请

US20120034606A1 DETECTION OF SALMONELLA ENTERICA SUBSPECIES ENTERICA SEROVAR ENTERITIDIS IN FOOD AND ENVIRONMENTAL SAMPLES, METHODS AND COMPOSITIONS THEREFOR 有权
标题翻译：食品安全食品中的食品和环境样品的检测方法及其组合物
公开(公告)号：US20120034606A1
公开(公告)日：2012-02-09
申请号：US13197758
申请日：2011-08-03
申请人： Robert TEBBS , Craig Cummings , Arlene Nunez , Priya Balachandran , Payman Fatemi , Olga Petrauskene
发明人： Robert TEBBS , Craig Cummings , Arlene Nunez , Priya Balachandran , Payman Fatemi , Olga Petrauskene
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2561/113 , C12Q2600/156 , Y02A50/451
摘要： Embodiments of the disclosure relate to compositions of isolated nucleic acid sequences, methods, workflows and kits of use thereof for detection of Salmonella enterica, subspecies enterica, serovar Enteritidis (S. Enteritidis) in a sample, particularly in a food (egg, poultry) or environmental sample (including poultry-related environmental samples). Embodiments of the disclosure may also be used to differentially detect S. Enteritidis without cross-reacting with S. Paratyphi C or other closely related Salmonella species. In some embodiments, methods and kits of the disclosure may comprise a TaqMan® assay. Following sample enrichment, methods of detection may be completed in approximately 3 hours.
摘要翻译：本公开的实施方案涉及分离的核酸序列的组合物，方法，工作流程和其用于检测肠炎沙门氏菌，肠亚科，血清型肠炎沙丁杆菌（肠炎沙丁杆菌）的样品中尤其是食品（鸡蛋，家禽）或环境样品（包括家禽相关环境样品）。本公开的实施方案也可用于差异检测肠杆菌，而不与副伤寒杆菌C或其他密切相关的沙门氏菌属物种交叉反应。在一些实施方案中，本公开的方法和试剂盒可以包含TaqMan测定。样品富集后，检测方法可在约3小时内完成。

4. 发明授权

US08795969B2 Detection of Listeria species in food and environmental samples, methods and compositions thereof 有权
标题翻译：食品和环境样品中利斯特氏菌物种的检测方法及其组合物
公开(公告)号：US08795969B2
公开(公告)日：2014-08-05
申请号：US13174622
申请日：2011-06-30
申请人： Olga Petrauskene , Craig Cummings , Paolo Vatta , Robert Tebbs , Priya Balachandran , Patrick Zoder , Lily Wong
发明人： Olga Petrauskene , Craig Cummings , Paolo Vatta , Robert Tebbs , Priya Balachandran , Patrick Zoder , Lily Wong
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/689 , C12Q2600/156 , C12Q2600/16
摘要： Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp. Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi. Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.
摘要翻译：本公开的实施方案涉及分离的核酸序列，其使用方法和用于检测样品中的几种利斯特氏菌物种的工作流程，特别是在食品或环境样品中。本公开的实施方案还可以用于彼此检测李斯特氏菌的一种或多种物种或菌株，例如，可以独立于其他李斯特菌属物种检测L.crilli。一些实施方案还描述了可以检测单核细胞增生李斯特氏菌（L. innocua），威尔逊氏乳杆菌（L.welshimeri），塞贝尔氏球菌（L.ceelgeri），马氏，（L.marthii）（以前称为incertae-sedis），伊卡诺菌（L. ivanovii）和L. 还描述了用于检测李斯特菌的试剂盒。在一些实施方案中，本公开的方法和试剂盒可以包括TAQMAN测定。在一些实施方案中，0.2-2cfu的李斯特菌属在24-28小时浓缩期后使用组合物，方法和试剂盒检测。

5. 发明申请

US20120009574A1 DETECTION OF LISTERIA SPECIES IN FOOD AND ENVIRONMENTAL SAMPLES, METHODS AND COMPOSITIONS THEREOF 有权
标题翻译：食品和环境样品中的LISTERIA物种的检测方法及其组合物
公开(公告)号：US20120009574A1
公开(公告)日：2012-01-12
申请号：US13174622
申请日：2011-06-30
申请人： Olga Petrauskene , Craig Cummings , Paolo Vatta , Robert Tebbs , Priya Balachandran , Patrick Zoder , Lily Wong
发明人： Olga Petrauskene , Craig Cummings , Paolo Vatta , Robert Tebbs , Priya Balachandran , Patrick Zoder , Lily Wong
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689 , C12Q2600/156 , C12Q2600/16
摘要： Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp. Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi. Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.
摘要翻译：本公开的实施方案涉及分离的核酸序列，其使用方法和用于检测样品中的几种利斯特氏菌物种的工作流程，特别是在食品或环境样品中。本公开的实施方案还可以用于彼此检测李斯特氏菌的一种或多种物种或菌株，例如，可以独立于其他李斯特菌属物种检测L.crilli。一些实施方案还描述了可以检测单核细胞增生李斯特氏菌（L. innocua），威尔逊氏乳杆菌（L.welshimeri），塞贝尔氏球菌（L.ceelgeri），马氏，（L.marthii）（以前称为incertae-sedis），伊卡诺菌（L. ivanovii）和L. 还描述了用于检测李斯特菌的试剂盒。在一些实施方案中，本公开的方法和试剂盒可以包括TAQMAN测定。在一些实施方案中，0.2-2cfu的李斯特菌属在24-28小时浓缩期后使用组合物，方法和试剂盒检测。

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