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1. 发明申请

US20070099293A1 Use of porous membrane to support developing conifer somatic embryos 审中-公开
标题翻译：使用多孔膜支持发育中的针叶树体细胞胚
公开(公告)号：US20070099293A1
公开(公告)日：2007-05-03
申请号：US11548951
申请日：2006-10-12
申请人： Pramod Gupta , Diane Holmstrom , Bonnie Larson , Susan Rayfield , Anthony Swanda
发明人： Pramod Gupta , Diane Holmstrom , Bonnie Larson , Susan Rayfield , Anthony Swanda
IPC分类号： A01H3/00
CPC分类号： A01H4/001 , A01H4/005 , C12M25/02
摘要： The present invention provides methods for developing conifer cotyledonary somatic embryos. In some embodiments, the methods of the invention include the step of culturing conifer pre-cotyledonary somatic embryos on a porous membrane, that is at least intermittently contacted with liquid development medium, for a period of time sufficient to produce conifer, cotyledonary, somatic embryos from the pre-cotyledonary somatic embryos.
摘要翻译：本发明提供了用于开发针叶子子叶体细胞胚的方法。在一些实施方案中，本发明的方法包括在多孔膜上培养针叶树子叶前体细胞胚的步骤，其至少间断地与液体显影培养基接触一段足以产生针叶树，子叶，体细胞胚的时间从子叶前体细胞胚。

2. 发明申请

US20050188436A1 Continuous culture of conifer embryogenic tissue 有权
标题翻译：针叶胚发生组织连续培养
公开(公告)号：US20050188436A1
公开(公告)日：2005-08-25
申请号：US11063416
申请日：2005-02-22
申请人： Pramod Gupta , Diane Holmstrom , Bonnie Larson
发明人： Pramod Gupta , Diane Holmstrom , Bonnie Larson
IPC分类号： A01H20060101 , A01H1/00 , A01H4/00 , A01H7/00 , C12N5/02 , C12N5/04 , C12N15/82
CPC分类号： A01H4/005 , A01H7/00 , C12N5/04
摘要： The present invention provides methods for multiplying conifer embryogenic tissue. The methods of the present invention each include the step of continuously culturing conifer embryogenic tissue in liquid multiplication medium for a period of time sufficient for the embryogenic tissue to multiply.
摘要翻译：本发明提供了用于倍增针叶胚发生组织的方法。本发明的方法各自包括在液体繁殖培养基中连续培养针叶树胚发生组织足以使胚发生组织繁殖的时间的步骤。

3. 发明申请

US20070101463A1 Use of porous membrane to support developing conifer somatic embryos 审中-公开
公开(公告)号：US20070101463A1
公开(公告)日：2007-05-03
申请号：US11548937
申请日：2006-10-12
申请人： Pramod Gupta , Diane Holmstrom , Bonnie Larson , Susan Rayfield , Anthony Swanda
发明人： Pramod Gupta , Diane Holmstrom , Bonnie Larson , Susan Rayfield , Anthony Swanda
IPC分类号： C12N5/04 , A01H7/00
CPC分类号： A01H4/001 , A01H4/005 , C12M25/02
摘要： The present invention provides methods for developing conifer cotyledonary somatic embryos. In some embodiments, the methods of the invention include the step of culturing conifer pre-cotyledonary somatic embryos on a porous membrane, that is at least intermittently contacted with liquid development medium, for a period of time sufficient to produce conifer, cotyledonary, somatic embryos from the pre-cotyledonary somatic embryos.

4. 发明申请

US20050026282A1 Methods and compositions for regrowth of cryopreserved conifer embryos 有权
标题翻译：冷冻针叶树胚胎再生长的方法和组成
公开(公告)号：US20050026282A1
公开(公告)日：2005-02-03
申请号：US10875666
申请日：2004-06-24
申请人： Pramod Gupta , Diane Holmstrom , Bonnie Larson , Doris Budworth
发明人： Pramod Gupta , Diane Holmstrom , Bonnie Larson , Doris Budworth
IPC分类号： A01H20060101 , A01H4/00 , A01N1/02 , A01N3/00 , C12N5/04 , A01H7/00 , C12N15/82
CPC分类号： A01H4/005 , A01H4/008 , A01N1/0226 , Y10T436/108331
摘要： The present invention provides methods for regrowth of conifer embryogenic cells. The methods comprise the steps of (a) contacting cryopreserved conifer embryogenic cells with a liquid transition medium, and (b) culturing the contacted conifer embryogenic cells in a regrowth medium to generate regrowth of the conifer embryogenic cells. Generally, the cryopreserved conifer embryogenic cells are contacted with the liquid transition medium for less than about 24 hours, such as for about one hour.
摘要翻译：本发明提供针叶树胚发生细胞再生长的方法。所述方法包括以下步骤：（a）使冷冻保存的针叶树胚胎发生细胞与液体转移培养基接触，和（b）在再生培养基中培养接触的针叶树胚发生细胞以产生针叶胚胎发生细胞的再生长。通常，冷冻保存的针叶树胚胎发生细胞与液体转移培养基接触少于约24小时，例如约1小时。

5. 发明申请

US20050026281A1 Development and stratification of pine somatic embryos using a liquid system 失效
标题翻译：使用液体系统的松体细胞胚的发育和分层
公开(公告)号：US20050026281A1
公开(公告)日：2005-02-03
申请号：US10875656
申请日：2004-06-24
申请人： Pramod Gupta , Diane Holmstrom , Bonnie Larson , Judith Zucati
发明人： Pramod Gupta , Diane Holmstrom , Bonnie Larson , Judith Zucati
IPC分类号： A01H4/00 , A01H7/00 , C12N5/04 , C12N15/82
CPC分类号： A01H4/005
摘要： The invention provides methods for producing pine somatic embryos using a liquid development medium and/or a liquid stratification medium. In a first aspect, the methods comprise the step of culturing embryogenic cells in, or on, a liquid development medium to produce cotyledonary pine somatic embryos. In another aspect, the methods comprise the step of culturing pine cotyledonary somatic embryos in, or on, a liquid stratification medium to produce stratified cotyledonary somatic embryos. The invention also provides methods for producing pine somatic embryos in bioreactors.
摘要翻译：本发明提供使用液体显影培养基和/或液体分层培养基生产松体细胞胚的方法。在第一方面，所述方法包括在液体显影培养基中或其上培养胚发生细胞以产生子叶松体细胞胚的步骤。另一方面，所述方法包括在液体分层培养基中或其上培养松果子体细胞胚以产生分层的子叶体细胞胚的步骤。本发明还提供了在生物反应器中生产松体细胞胚的方法。

6. 发明申请

US20050003415A1 Media and methods for promoting maturation of conifer somatic embryos 有权
标题翻译：用于促进针叶树体细胞胚成熟的培养基和方法
公开(公告)号：US20050003415A1
公开(公告)日：2005-01-06
申请号：US10861168
申请日：2004-06-04
申请人： Pramod Gupta , Bonnie Larson , Diane Holmstrom
发明人： Pramod Gupta , Bonnie Larson , Diane Holmstrom
IPC分类号： A01H4/00 , C12N5/00 , C12N5/02 , C12Q1/68
CPC分类号： A01H4/005
摘要： In one aspect, the present invention provides methods for producing mature conifer somatic embryos. The methods of the invention comprise the steps of: (a) culturing pre-cotyledonary conifer somatic embryos in, or on, a development medium comprising less than 0.5% of gellan gum to form cotyledonary conifer somatic embryos; and (b) culturing cotyledonary conifer somatic embryos formed in step (a) in, or on, a maturation medium comprising between about 0.5% to about 1.5% of gellan gum to form mature conifer somatic embryos. In some embodiments, the maturation medium further comprises sucrose and/or abscisic acid.
摘要翻译：一方面，本发明提供了生产成熟针叶树体细胞胚的方法。本发明的方法包括以下步骤：（a）在包含少于0.5％吉兰糖胶的显影培养基中或上培养前叶子针叶体细胞胚，以形成子叶针叶体细胞胚; 和（b）在步骤（a）中形成的子叶针叶树体细胞胚在包含约0.5％至约1.5％的结冷糖胶的成熟培养基中或之上培养，以形成成熟的针叶树体细胞胚。在一些实施方案中，成熟培养基还包含蔗糖和/或脱落酸。

7. 发明申请

US20050198713A1 Methods for developing conifer somatic embryos 有权
标题翻译：发展针叶树体细胞胚的方法
公开(公告)号：US20050198713A1
公开(公告)日：2005-09-08
申请号：US11052361
申请日：2005-02-07
申请人： Pramod Gupta , Diane Holmstrom , Bonnie Larson
发明人： Pramod Gupta , Diane Holmstrom , Bonnie Larson
IPC分类号： A01H4/00 , A01H7/00 , C12N5/04 , C12N15/82
CPC分类号： A01H4/005
摘要： The present invention provides methods for producing conifer cotyledonary somatic embryos. The methods of the present invention each includes the step of culturing embryogenic conifer tissue in, or on, a development medium for a period of time sufficient to produce conifer cotyledonary somatic embryos from the embryogenic conifer tissue, wherein the volume of development medium is in the range of from about 35 ml to about 50 ml per 0.5 mL of embryogenic conifer tissue.
摘要翻译：本发明提供了生产针叶子子叶体细胞胚的方法。本发明的方法各自包括在显影培养基中或培养基上培养胚发生针叶树组织足以从胚性针叶树组织产生针叶树子叶体细胞胚的时间的步骤，其中显影培养基的体积在范围为每0.5mL胚性针叶树组织约35ml至约50ml。

8. 发明授权

US07888099B2 Methods for producing a synchronized population of conifer somatic embryos 有权
标题翻译：生产针叶树体细胞胚的同步群体的方法
公开(公告)号：US07888099B2
公开(公告)日：2011-02-15
申请号：US10636081
申请日：2003-08-06
申请人： Pramod K. Gupta , Diane Holmstrom , Bonnie Larson
发明人： Pramod K. Gupta , Diane Holmstrom , Bonnie Larson
IPC分类号： A01H4/00 , A01H1/00
CPC分类号： A01H4/005
摘要： In one aspect, the present invention provides methods for producing conifer somatic embryos. The methods of this aspect of the invention each include the steps of cultivating conifer embryogenic cells in, or on, a synchronization medium that comprises an absorbent composition and at least one synchronization agent selected from the group consisting of abscisic acid and a gibberellin. Some embodiments of the methods provide a synchronized population of conifer somatic embryos wherein at least about 50% of the embryos are at about the same developmental stage. Some embodiments of the methods of the invention yield at least 100% more cotyledonary somatic embryos than an identical method for producing conifer somatic embryos that does not utilize a synchronization medium comprising an absorbent composition and at least one synchronization agent selected from the group consisting of abscisic acid and a gibberellin.
摘要翻译：一方面，本发明提供了生产针叶树体细胞胚的方法。本发明的这个方面的方法各自包括在含有吸收组合物和选自脱落酸和赤霉素的至少一种同步剂的同步培养基中或其上培养针叶树胚发生细胞的步骤。所述方法的一些实施方案提供了针叶树体细胞胚的同步群体，其中至少约50％的胚胎处于大致相同的发育阶段。本发明方法的一些实施方案比用于生产针叶树体细胞胚的相同方法产生至少100％更多的子叶体细胞胚，其不利用包含吸收组合物的同步培养基和至少一种选自脱落的同步剂酸和赤霉素。

9. 发明授权

US07521237B2 Methods for promoting maturation of conifer somatic embryos 有权
标题翻译：促进针叶体细胞胚成熟的方法
公开(公告)号：US07521237B2
公开(公告)日：2009-04-21
申请号：US10861168
申请日：2004-06-04
申请人： Pramod K. Gupta , Bonnie Larson , Diane Holmstrom
发明人： Pramod K. Gupta , Bonnie Larson , Diane Holmstrom
IPC分类号： C12N5/00
CPC分类号： A01H4/005
摘要： In one aspect, the present invention provides methods for producing mature conifer somatic embryos. The methods of the invention comprise the steps of: (a) culturing pre-cotyledonary conifer somatic embryos in, or on, a development medium comprising less than 0.5% of gellan gum to form cotyledonary conifer somatic embryos; and (b) culturing cotyledonary conifer somatic embryos formed in step (a) in, or on, a maturation medium comprising between about 0.5% to about 1.5% of gellan gum to form mature conifer somatic embryos. In some embodiments, the maturation medium further comprises sucrose and/or abscisic acid.
摘要翻译：一方面，本发明提供了生产成熟针叶树体细胞胚的方法。本发明的方法包括以下步骤：（a）在包含少于0.5％吉兰糖胶的显影培养基中或上培养前叶子针叶体细胞胚，以形成子叶针叶体细胞胚; 和（b）在步骤（a）中形成的子叶针叶树体细胞胚在包含约0.5％至约1.5％的结冷糖胶的成熟培养基中或之上培养，以形成成熟的针叶树体细胞胚。在一些实施方案中，成熟培养基还包含蔗糖和/或脱落酸。

10. 发明授权

US07381562B2 Methods for producing cotyledonary pine embryos utilizing a gibberellin 有权
标题翻译：用赤霉素生产子叶松胚胎的方法
公开(公告)号：US07381562B2
公开(公告)日：2008-06-03
申请号：US10405819
申请日：2003-04-01
申请人： Pramod K. Gupta , Diane Holmstrom , Bonnie Larson
发明人： Pramod K. Gupta , Diane Holmstrom , Bonnie Larson
IPC分类号： C12N5/00 , C12N5/04 , A01H4/00
CPC分类号： A01H4/005
摘要： The present invention provides methods for producing cotyledonary pine embryos. The methods of the invention each include the step of culturing embryogenic pine tissue in, or on, a medium including at least one gibberellin to yield cotyledonary pine embryos. In some embodiments, an absorbent composition, such as activated charcoal, is included in the medium to further promote production of cotyledonary pine embryos.
摘要翻译：本发明提供了生产子叶松胚胎的方法。本发明的方法各自包括在包含至少一种赤霉素的培养基中培养胚发生松树组织以产生子叶松胚胎的步骤。在一些实施方案中，介质中包含吸收剂组合物，例如活性炭，以进一步促进子叶松胚胎的产生。

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